水体中Cu2+和Cd2+污染对育珠蚌肝脏中3种酶的影响 |
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引用本文: | 魏炜,刘克武,赵欣平,石安静,喻东. 水体中Cu2+和Cd2+污染对育珠蚌肝脏中3种酶的影响[J]. 应用与环境生物学报, 2004, 10(2): 170-173 |
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作者姓名: | 魏炜 刘克武 赵欣平 石安静 喻东 |
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作者单位: | 四川大学生命科学学院,成都,610064 |
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基金项目: | 国家自然科学基金(No.39970577)资助项目~~ |
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摘 要: | 选取两种水体中常见的重金属污染离子(Cu^2 和Cd^2 ),按一定的浓度梯度配制成溶液,并以此溶液饲养育珠蚌一定时间后,测定两种离子对育珠蚌肝脏中的酯酶(EST)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性的影响.结果表明,低浓度的重金属离子可以诱导3种酶活性的升高,而高浓度的离子则抑制酶的活性.同工酶电泳检测显示,高浓度的离子导致EST同工酶谱减少一条谱带,低浓度的离子则诱导产生新的SOD同工酶类型,但两种离子均未对POD同工酶谱产生影响.图6参10
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关 键 词: | 水体 Cu^2+ Cd^2+ 污染 育珠蚌 肝脏 同工酶 酯酶 过氧化物酶 超氧化物歧化酶 重金属 |
修稿时间: | 2003-09-19 |
EFFECT OF Cu2+ AND Cd2+ ON THREE ENZYMES IN OYSTER LIVER |
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Abstract: | The effects of two heavy metal ions (Gu2 and Cd2 ) on the activities of EST, POD , SOD in oyster liver were studied. The results showed that low concentrations (0. 25 mg L-1 and 0. 5 mg L-1) of the ions could improve the activities of the three enzymes, but high concentrations (0.75 mg L-1 and 1 mg L-1 ) could inhibit the activities. Research of the two ions' effect on the three enzymes 'isoenzyme implied that high concentration of ions could make EST-isoenzyme lose one band, but low concentration had no effect. The effect on SOD-isoenzyme was opposite. High concentration made no effect, while low one could induce new kind of SOD-isoenzyme. But the two heavy ions could not affect POD-isoenzyme. These results revealed that both the enzyme activities and the kinds of isoenzymes would obviously change following the alteration of the ion concentration. Fig 6, Ref 10 |
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Keywords: | oyster Cu2 Cd2 isoenzyme EST POD SOD |
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