Affiliation: | (1) Dpto Bioquímica, Universidad de la Habana, Calle 25 entre J e I #455, Vedado Plaza, P.C. 10400 Havana, Cuba;(2) Departamento de Biología Funcional, Laboratorio de Genética Acuícola, Universidad de Oviedo, 33071 Oviedo, Spain |
Abstract: | Genetic variability within the Cuban population of the white shrimp Litopenaeus schmitti (Burkenroad, 1936) was assessed using five microsatellite loci and samples collected from four sites (Batabanó, Cienfuegos, Tunas de Zaza, and Manzanillo) from November 1999 to March 2000. All loci were polymorphic, and a total of 80 alleles were found, 13 of them private alleles occurring only in a single population and always in low frequencies. The Cienfuegos sample had the highest observed heterozygosity (Ho=0.653), and the Tunas de Zaza sample had the lowest values (Ho=0.605), but there were no significant differences among samples in heterozygosity or in the mean numbers of alleles per locus (ranging from 11.0 to 11.6). Significant differentiation among populations was detected (FST=0.012, P<0.001). Low but significant FST values were revealed in pairwise comparisons between populations. Assignation tests correctly assigned high percentages of individuals to their original populations (74.5%) using a Bayesian approach. The significant differentiation among populations could be due to the restriction of gene flow among populations of L. schmitti and is concordant with previous allozyme studies on Cuban populations.Communicated by J.P. Grassle, New Brunswick |