Atrazine,chlorpyrifos, and iprodione effect on the biodiversity of bacteria,actinomycetes, and fungi in a pilot biopurification system with a green cover |
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| Authors: | Sebastian Elgueta Arturo Correa Marco Campo Felipe Gallardo Dimitrios Karpouzas Maria Cristina Diez |
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| Institution: | 1. Biotechnological Research Center Applied to the Environment (CIBAMA), Universidad de La Frontera, Temuco, Chile;2. Department of Environment and Sustainability, Instituto de Investigaciones Agropecuarias, CRI La Platina, Santiago, Chile;3. Department of Environment and Sustainability, Instituto de Investigaciones Agropecuarias, CRI La Platina, Santiago, Chile;4. Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Viopolis, Greece;5. Chemical Engineering Department, University of La Frontera, Temuco, Chile |
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| Abstract: | The use of biopurification systems can mitigate the effects of pesticide contamination on farms. The primary aim of this study was to evaluate the effect of pesticide dissipation on microbial communities in a pilot biopurification system. The pesticide dissipation of atrazine, chlorpyrifos and iprodione (35 mg kg?1 active ingredient a.i.]) and biological activity were determined for 40 days. The microbial communities (bacteria, actinomycetes and fungi) were analyzed using denaturing gradient gel electrophoresis (DGGE). In general, pesticide dissipation was the highest by day 5 and reached 95%. The pesticides did not affect biological activity during the experiment. The structure of the actinomycete and bacterial communities in the rhizosphere was more stable during the evaluation than that in the communities in the control without pesticides. The rhizosphere fungal communities, detected using DGGE, showed small and transitory shifts with time. To conclude, rhizosphere microbial communities were not affected during pesticide dissipation in a pilot biopurification system. |
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| Keywords: | Biopurification system pesticide dissipation biological activity DGGE |
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