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| 铜绿假单胞菌胞内酶粗提液对十溴联苯醚的降解 | |
| 引用本文： | 史广宇,尹华,叶锦韶,彭辉,张娜,何宝燕.铜绿假单胞菌胞内酶粗提液对十溴联苯醚的降解[J].环境科学,2013,34(4):1517-1523. |
| 作者姓名： | 史广宇 尹华 叶锦韶 彭辉 张娜 何宝燕 |
| 作者单位： | 1. 中国科学院广州地球化学研究所,广州,510640 2. 华南理工大学环境科学与工程学院,广州 510006;工业聚集区污染控制与生态修复教育部重点实验室,广州 510006 3. 暨南大学环境工程系,广州,510632 |
| 基金项目： | 国家自然科学基金委-广东省联合基金重点项目(U0933002); 国家自然科学基金项目(50978122); 广州市科技计划项目(2011J4300075) |
| 摘 要： | 探讨了铜绿假单胞菌粗酶液对十溴联苯醚(BDE-209)的降解特性,结果表明,该菌株胞内酶在12 h内对1 mg.L-1的BDE-209降解率达到了69.22%,温度、pH值、酶蛋白浓度及底物BDE-209浓度均会影响胞内酶粗提液对BDE-209的降解效率.当BDE-209浓度为1 mg.L-1时,BDE-209适宜的酶促降解条件为:温度30℃,反应pH值7.5,且降解率随着酶浓度的增加而增大.胞内酶对BDE-209的降解过程符合一级动力学反应模型,底物浓度为1 mg.L-1时降解速率最快,半衰期为6.9 h.胞内酶降解BDE-209符合高浓度底物抑制的酶促反应类型,其基本降解动力学参数为rmax=0.133 mg.(L.h)-1,Km=0.642mg.L-1,Ki=1.558 mg.L-1. |
| 关 键 词： | 铜绿假单胞菌 胞内酶 十溴联苯醚 酶降解 动力学 |
| 收稿时间： | 2012/7/29 0:00:00 |
| 修稿时间： | 2012/9/10 0:00:00 |
Biodegradation of Decabromodiphenyl Ether by Intracellular Enzyme Obtained from Pseudomonas aeruginosa |
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| SHI Guang-yu,YIN Hu,YE Jin-shao,PENG Hui,ZHANG Na and HE Bao-yan.Biodegradation of Decabromodiphenyl Ether by Intracellular Enzyme Obtained from Pseudomonas aeruginosa[J].Chinese Journal of Environmental Science,2013,34(4):1517-1523. | |
| Authors: | SHI Guang-yu YIN Hu YE Jin-shao PENG Hui ZHANG Na and HE Bao-yan |
| Institution: | Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China;College of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006, China;The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education,Guangzhou 510006, China;Department of Environmental Engineering, Jinan University, Guangzhou 510632, China;Department of Environmental Engineering, Jinan University, Guangzhou 510632, China;Department of Environmental Engineering, Jinan University, Guangzhou 510632, China;Department of Environmental Engineering, Jinan University, Guangzhou 510632, China |
| Abstract: | |
| Keywords: | Pseudomonas aeruginosa intracellular enzyme decabromodiphenyl ether (BDE-209) enzymatic degradation kinetics |
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