| 酸改性壳聚糖载体对漆酶的固定化 |
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| 引用本文： | 朱启忠,杨万芹.酸改性壳聚糖载体对漆酶的固定化[J].资源开发与市场,2010,26(5):388-390,394. |
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| 作者姓名： | 朱启忠 杨万芹 |
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| 作者单位： | 山东大学威海分校,海洋学院,山东,威海,264209 |
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| 基金项目： | 威海市海洋研究院课题 |
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| 摘 要： | 利用有机酸改性壳聚糖和交联法制备酸化壳聚糖载体,然后用改性壳聚糖载体固定漆酶。结果表明,甲酸、酒石酸改性壳聚糖的最适条件是壳聚糖与酸的量比分别为100/1(g/mol)、100/0.5(g/mol),戊二醛的浓度分别为1%、2%,缓冲溶液的pH分别为4.4、3.6,反应时间分别为3h、4.5h。两种酸改性的壳聚糖用于漆酶的固定,其酶活都有所提高,尤其用酒石酸改性的壳聚糖载体的效果最好,其酶活提高了57%。
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| 关 键 词： | 壳聚糖 甲酸 酒石酸 漆酶 固定化 |
Modification of Chitosan Carrier for lmmobilization of Laccase |
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| ZHU Qi-zhong,YANG Wan-qin.Modification of Chitosan Carrier for lmmobilization of Laccase[J].Resource Development & Market,2010,26(5):388-390,394. |
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| Authors: | ZHU Qi-zhong YANG Wan-qin |
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| Institution: | ZHU Qi-zhong,YANG Wan-qin(Marine College,Sh,ong University at Weihai,Weihai 264209,China) |
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| Abstract: | The use of organic acid-modified chitosan,and use of cross-linked chitosan prepared by acidification of the carrier,and then modified chitosan immobilized laccase.Results as following:Formic acid,tartaric acid modified chitosan optimum conditions were that the molar ratio of chitosan and acid were 100 / 1(g/mol),100/0.5(g/mol);the concentration of glutaraldehyde were 1%,2%;the pH of buffer solution were 4.4,3.6;reaction time were 3h,4.5h.Modification of chitosan of two kinds of acid used in the fixed laccas... |
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| Keywords: | chitosan methane acid tartaric acid laccase immobilization |
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