PBNA及其同分异构体PANA在TA-1系小鼠切除一侧肾脏后的致癌性研究

对切除一侧肾脏的TA-1系雄性小鼠,多次皮下注射PBNA或它的同分异构体PANA的二甲基亚砜溶液,总剂量达304—328mg,实验周期在9个月左右时,均能引起显著高于对照小鼠的肾血管内皮肉瘤.实验再次证明.工业防老剂A及其主要成份PANA对小鼠有致癌性.国内外的橡胶行业对工业防老剂A还从未怀疑过有致癌作用,迄今尚未见这类报道,因此值得有关方面引起重视.切除一侧肾脏后引癌,不仅能缩短实验周期,还能减少致癌物的用量,为今后研究整体动物引癌实验提供了改良的线索.

A COMPARATIVE STUDY ON CARCINOGENIC ACTIVITY OF PBNA AND PANA IN UNILATERALLY NEPHRECTOMIZED TA-l MICE

Repeated subcutaneous injections of DMSO-dissolved technical or chemically pure PBNA and its corresponding isomer PANA all induced in male young adult TA-1 mice significantly higher incidence of malignancy than controls. Total doses given amounted to 304-328mg. At the end of the 9th month malignant tumors were induced in all of four groups. Incidence of hemangiosarcoma of the kidney was as follows: nephrectomy + technical PBNA: 76.2%; nephrectomy+pure PBNA: 47.1%; nephrectomy+technical PANA: 100%; nephrectomy+pure PANA:75%. More pulmonary carcinoma were induced in both PBNA groups than in PANA groups. Technical PBNA in DMSO injected repeatedly to TA-1 mice induced pulmonary carcinoma in 21.1% of mice. Technical PANA induced hemangiosarcoma of the kidney in 36.8% of mice. It was shown that both technical PBNA (neoZone D) and technical PANA (neozone A) were carcinogenic to TA-1 mice with different orgnotropism while previous unilateral nephrectomy increased the susceptibility of the another kidney to the induction of hemangiosacoma.