| MTT法测定大肠杆菌活菌数实验研究 |
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| 引用本文： | 汪志荣,高琼,马传鑫,李燕妮.MTT法测定大肠杆菌活菌数实验研究[J].环境科学学报,2011,31(12):2642-2650. |
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| 作者姓名： | 汪志荣 高琼 马传鑫 李燕妮 |
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| 作者单位： | 天津理工大学环境科学与安全工程学院,天津,300384 |
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| 摘 要： | 实验分析MTT比色法中的工作波长、反应时间、MTT用量、菌龄、二甲基亚砜（DMSO）等因素对大肠杆菌活细菌计数的影响.同时,通过与平板计数对比,确定了MTT细菌计数的可行性.结果表明,当细菌数在107～109cell·mL-1范围内时,双波长MTT法可用于检测大肠杆菌的活菌数量,最佳反应时间为60min,MTT溶液用量...
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| 关 键 词： | MTT 双波长测定 大肠杆菌 活菌计数 |
| 收稿时间： | 2/25/2011 9:48:05 PM |
| 修稿时间： | 6/13/2011 7:55:42 PM |
Experimental study on the application of MTT colorimetric method in counting live Escherichia coli |
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| WANG Zhirong,GAO Qiong,MA Chuanxin and LI Yanni.Experimental study on the application of MTT colorimetric method in counting live Escherichia coli[J].Acta Scientiae Circumstantiae,2011,31(12):2642-2650. |
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| Authors: | WANG Zhirong GAO Qiong MA Chuanxin and LI Yanni |
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| Institution: | School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384,School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384,School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384 and School of Environmental Science and Safety Engineering, Tianjin University of Technology, Tianjin 300384 |
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| Abstract: | |
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| Keywords: | MTT Dual wavelength measurement Escherichia coli Live germ counting |
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