Identification of protein biomarkers of mercury toxicity in fish |
| |
Authors: | José Cavalcante Souza Vieira Camila Pereira Braga Grasieli de Oliveira Aline de Lima Leite João Vitor de Queiroz Bruna Cavecci Alis Correia Bittarello Marília Afonso Rabelo Buzalaf Luiz Fabricio Zara Pedro de Magalhães Padilha |
| |
Affiliation: | 1.Institute of Biosciences,S?o Paulo State University (UNESP),Botucatu,Brazil;2.University of Nebraska-Lincoln,Lincoln,USA;3.School of Veterinary Medicine and Animal Science,S?o Paulo State University (UNESP),Botucatu,Brazil;4.University of S?o Paulo (USP),Bauru,Brazil;5.College of Planaltina,University of Brasília (UnB),Federal District,Brazil |
| |
Abstract: | Bioaccumulative metals such as mercury are found in increasing amounts in fish and their consumers. In the region of the Madeira River, in the Brazilian Amazon, mercury (Hg) is a predominant contaminant in the aquatic ecosystem. There is therefore a need to find specific biomarkers of mercury toxicity in fish to monitor contaminations. Here, mercury-bound proteins were identified in the liver tissues of fishes Mylossoma duriventre and Brachyplatystoma rousseauxii. Mercury was quantified in liver tissue, pellets and protein spots by graphite furnace atomic absorption. Proteins were fractionated by two-dimensional polyacrylamide gel electrophoresis and identified by mass spectrometry with electrospray ionization. We identified nine proteins linked to mercury and that presented biomarker characteristics of mercury. Among the proteins identified, isoforms of parvalbumin, ubiquitin-40S ribosomal protein S27a, brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2 and betaine–homocysteine S-methyltransferase 1 are notable for having the molecular function of binding to metallic ions. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|