Screening of agonistic activities against four nuclear receptors in wastewater
treatment plants in Japan using a yeast two-hybrid assay |
| |
Authors: | Daisuke Inoue Koki Nakam Kazuko Sawad Taro Watanabe Hisae Matsui Kazunari Sei Tsuyoshi Nakanishi and Michihiko Ike |
| |
Institution: | 1. Division of Sustainable Energy and Environmental Engineering,Osaka University,2-1 Yamadaoka,Suita,Osaka 565-0871,Japan 2. Laboratory of Hygienic Chemistry and Molecular Toxicology,Gifu Pharmaceutical University,5-6-1 Mitahora-higashi,Gifu 502-8585,Japan |
| |
Abstract: | To assess the potential endocrine disruptive e ects through multiple nuclear receptors (NRs), especially non-steroidal NRs, in
municipal wastewater, we examined the agonistic activities on four NRs (estrogen receptor , thyroid hormone receptor , retinoic acid
receptor and retinoid X receptor ) of untreated and treated wastewater from municipal wastewater treatment plants (WWTPs) in
Japan using a yeast two-hybrid assay. Investigation of the influent and e uent of seven WWTPs revealed that agonistic activities against
steroidal and non-steroidal NRs were always detected in the influents and partially remained in the e uents. Further investigation of
four WWTPs employing conventional activated sludge, pseudo-anoxic-oxic, anoxic-oxic and anaerobic-anoxic-oxic processes revealed
that the ability to reduce the agonistic activity against each of the four NRs varies depending on the treatment process. These results
indicated that municipal wastewater in Japan commonly contains endocrine disrupting chemicals that exert agonistic activities on
steroidal and non-steroidal NRs, and that some of these chemicals are released into the natural aquatic environment. Although the
results obtained in yeast assays suggested that measured levels of non-steroidal NR agonists in the e uent of WWTPs were not likely
to cause any biological e ect, further study is required to assess their possible risks in detail. |
| |
Keywords: | agonistic activity endocrine disrupting chemicals non-steroidal nuclear receptor wastewater treatment pla |
本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录! |
| 点击此处可从《环境科学学报(英文版)》浏览原始摘要信息 |
| 点击此处可从《环境科学学报(英文版)》下载免费的PDF全文 |
|