Primary co-culture as a complementary approach to explore the diversity of bacterial associations in marine invertebrates: the example of <Emphasis Type="Italic">Nautilus macromphalus</Emphasis> (Cephalopoda: Nautiloidea) |
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Authors: | Mathieu Pernice Delphine Pichon Isabelle Domart-Coulon Jocelyne Favet Renata Boucher-Rodoni |
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Institution: | (1) Muséum National d’Histoire Naturelle, Département Peuplements et Milieux Aquatiques, Biologie des Organismes Marins et Ecosystèmes (UMR BOME 5178 CNRS), 55 rue Buffon, 75005 Paris, France;(2) Laboratory of Molecular Biology of Higher Plants (LBMPS), University of Geneva, Quai Ernest-Ansermet 30, 1211 Geneva, Switzerland |
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Abstract: | The recent application of molecular tools to address associations between bacteria and marine invertebrates has provided access
to an immense diversity of unidentified microbes resistant to cultivation. However, the role of bacteria as partners in animal
physiology remains unclear and in most cases difficult to investigate in the absence of adequate condition of cell growth
and proliferation. In this work, we studied the reservoir of microbes associated with the excretory organs of Nautilus macromphalus as a model. Using the bacterial 16S RNA gene as a marker, we compared three complementary approaches for bacterial detection:
bacterial DNA extraction from N. macromphalus tissues (“molecular approach”), strain isolation to provide a bacterial culture collection (“microbiological approach”) and
finally, maintenance of N. macromphalus excretory organ cells with associated bacteria (“cellular approach”). Our results stress the potential of the “cellular approach”
as a promising new tool as it promotes the detection of as yet uncultured β-proteobacteria and spirochaetes associated with
N. macromphalus, and serves as a foundation for future studies describing potential roles that these bacteria may play in Nautilus. |
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