| 应用黑褐新糠虾测试织纹螺毒性的初步研究 |
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| 引用本文： | 李爱峰,于仁成,李钧,王云峰,颜天,周名江.应用黑褐新糠虾测试织纹螺毒性的初步研究[J].海洋环境科学,2007,26(2):112-115,120. |
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| 作者姓名： | 李爱峰 于仁成 李钧 王云峰 颜天 周名江 |
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| 作者单位： | 1. 中国海洋大学,海洋环境与生态教育部重点实验室,山东,青岛,266100;中科院海洋研究所海洋生态与环境科学重点实验室,山东,青岛,266071
2. 中国海洋大学,海洋环境与生态教育部重点实验室,山东,青岛,266100 |
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| 基金项目： | 国家重点基础研究发展计划(973计划)
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国家科技攻关项目 |
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| 摘 要： | 尝试以黑褐新糠虾作为实验生物,研究了投喂不同毒性的织纹螺对糠虾存活的影响,并探讨了这一方法在织纹螺毒性测试中的应用.实验结果表明:有毒织纹螺能够导致黑褐新糠虾中毒死亡,糠虾的半致死时间与织纹螺的毒性之间有显著的相关性(R2=0.9943),在96 h内织纹螺对糠虾的半致死毒性为40.11 MU/g.利用黑褐新糠虾作为实验生物可以测试织纹螺样品毒性的高低,方法的检出限约为10.44 MU/g,与日本法定的河豚鱼食用安全标准(10 MU/g)相当.
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| 关 键 词： | 织纹螺 黑褐新糠虾 河豚毒素(TTX) 毒性测试 应用 黑褐新糠虾 毒性测试 织纹螺 致死毒性 研究 snail bioassay toxicity study primary 安全标准 河豚鱼 日本 样品 利用 相关性 致死时间 中毒死亡 结果 |
| 文章编号： | 1007-6336（2007）02-0112-04 |
| 收稿时间： | 2005-08-16 |
| 修稿时间： | 2005-08-162005-10-13 |
A primary study on toxicity bioassay of snail (Nassarius sp.) samples using mysis shrimp (Neomysis awatschensis) |
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| LI Ai-feng,YU Ren-cheng,LI Jun,WANG Yun-feng,YAN Tian,ZHOU Ming-jiang.A primary study on toxicity bioassay of snail (Nassarius sp.) samples using mysis shrimp (Neomysis awatschensis)[J].Marine Environmental Science,2007,26(2):112-115,120. |
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| Authors: | LI Ai-feng YU Ren-cheng LI Jun WANG Yun-feng YAN Tian ZHOU Ming-jiang |
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| Institution: | 1. Key Laboratory of Marine Environmental Sciences and Ecology, Ocean University of China, Qingdao, 266100, China;2. Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, CAS, Qingdao 266071, China |
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| Abstract: | In this paper,a bioassay method using mysis shrimp Neomysis awatschensis was used to test its potential in monitoring toxicity of the snails Nassarius sp..The shrimps were fed with homogenized Nassarius tissues with different toxicity to find out the relationship between the toxicity of snail samples and the mortality of shrimps.The results showed that toxic Nassarius samples could lead to the mortality of Neomysis awatschensis,and there was a good linearity between the observed LT50 and the toxicity of Nassarius samples(R2=0.9943).Therefore,the Neomysis awatschensis could be used as the test organism to evaluate the toxicity of Nassarius samples based on the procedure developed,and the detection limit was about 10.44 MU/g,which is comparable to the safety criteria for consumption of puffer fishes in Japan(10 MU/g). |
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| Keywords: | Nassarius sp Neomysis awatschensis tetrodotoxin(TTX) toxicity bioassay |
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