| 现代分子生物学技术在动物肠道微生物多样性研究中的应用 |
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| 引用本文： | 冯霞,殷幼平,王中康. 现代分子生物学技术在动物肠道微生物多样性研究中的应用[J]. 应用与环境生物学报, 2005, 11(3): 381-387 |
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| 作者姓名： | 冯霞 殷幼平 王中康 |
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| 作者单位： | 重庆大学生物工程学院,教育部生物力学与组织工程重点实验室,重庆,400044 |
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| 基金项目： | 重庆市自然科学基金资助(渝科发计字2002-17号)~~ |
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| 摘 要： | 现代分子生物技术的蓬勃发展解决了不可培养微生物研究的难题,使得肠道微生物的研究进入一个新的阶段.本文主要介绍了基于16SrRNA的分子分析技术,包括DGGE(变性梯度凝胶电泳),TGGE(温度梯度凝胶电泳),SSCP(单链构象多态性),RFLP(限制性片段长度多态性)等肠道微生物研究工作中常用的分子生物学技术方法、适用范围及可能的发展方向,为动物肠道微生物区系的进一步研究及开发应用提供帮助.表1参42
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| 关 键 词： | 16S rRNA 16S-23S rDNA间区 遗传指纹图谱技术 肠道微生物多样性 |
| 修稿时间： | 2004-08-11 |
APPLICATION OF MOLECULAR BIOLOGICAL TECHNIQUES IN RESEARCH OF MICROBIAL DIVERSITY IN ANIMAL INTESTINES |
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| FENG Xia,YIN Youping,WANG Zhongkang. APPLICATION OF MOLECULAR BIOLOGICAL TECHNIQUES IN RESEARCH OF MICROBIAL DIVERSITY IN ANIMAL INTESTINES[J]. Chinese Journal of Applied and Environmental Biology, 2005, 11(3): 381-387 |
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| Authors: | FENG Xia YIN Youping WANG Zhongkang |
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| Abstract: | Molecular biological techniques were applied in micro-ecological research and resolved lots of difficulties in studying the microorganisms, which could not be cultured. So the research of intestine microbes gained a new phase. This paper presents a mini-review on the molecular ecological techniques used for microbial diversity research in animal guts based on 16S rRNA, as well as 16S-23S rDNA Intergenic Spacer Region, RAPD, LMW RNA, fluorescence in situ hybridization, including a series of genetic fingerprint profile techniques, such as DGGE (denaturing gradient gel electrophoresis), TGGE (temperature gradient gel electrophoresis), SSCP (single-stranded-conformation polymorphism ) and RFLP (restriction fragment length polymorphisms). Tab 1, Ref 42 |
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| Keywords: | 16S rRNA 16S-23S rDNA ISR genetic fingerprint profile technique intestine microbial diversity |
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