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| 环境内分泌干扰物对河川沙塘鳢的雌激素效应检测 |
| Detecting ofthe Estrogen Effectin Gobbid Fish (O dontobutis potam ophila) in Responseto Endocrine-D isrupting Contam inants |
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| DOI： |
| 中文关键词: 环境内分泌干扰物 河川沙塘鳢 ELLSA 雌二醇 壬基酚 太湖 |
| 英文关键词: Endocrine-Disrupting Contaminants(EDCs) Gobbid Fish (Odontobutis potam ophila) ELL SA Estradiol NonlyPhenol Taihu Lake |
| 基金项目:中国水产科学研究院内陆渔业生态环境和资源重点开放实验室开放基金资助项目 |
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| 中文摘要: |
| 阐述了从河川沙塘鳢 (Odontobutis potam ophila) 卵巢中提取卵黄磷脂蛋白, 以及制备兔抗卵黄磷脂蛋白抗血清的过程, 介绍了用此血清建立的河川沙塘鳢血清卵黄蛋白原间接竞争ELISA检测方法。以卵黄磷脂蛋白为参照, 此法的最低检测质量浓度为40 μ g/L, 最佳工作范围为160 μ g/L ～ 1 280 μ g/L 。使用此法检测雌二醇 (E 2 ) 、壬基酚 (NP) 对雄性河川沙塘鳢的卵黄蛋白原诱导效果时, 前者的最低有效质量浓度是 0.001 mg/kg(E 2 / 鱼质量 |
| 英文摘要: |
| Lipovitellin extracted from the ovariesofgobbid fish (Odontobutis potam ophila) isused to pre parerabbitantiserum. The method ofindirectcompetitiveenzyme- linkedimmunosorbentassay (ELISA) wases tablishedto detecttheserum vitellogenin. Lipovitellin wasused asacomparativestandard. Thelowestdetectable concentration was40 μ g/L andtheoptimaldetermination rangeisbetween160 μ g/L ～ 1280 μ g/L. Thelowest effectualconcentration forE 2 is0.001 mg/kg(E 2 /fish mass) and50 mg/kg(NP/fish mass) forNP, valuesob tained throughthetestingoftheinduced effectofvitellogenin ofE 2 and NPin male gobbid fish. Experimentalre sultsshow, when fish are exposed to NP contaminants,theconcentration ofNP in theirbodiesreaches200 mg/kg (NP/fish mass) and asharp vitellogeninincreasein serum isdetected. During a yearlongecologicalinvesti gation in Dong Shan estuary of Taihu Lake,it wasdiscovered that50% ofthe samplescollected in March and 35% ofthe samplescollectedin Aprilweretested positive forNP contaminants. |
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