特别选题
曾薇,杨庆,张树军,马勇,刘秀红,彭永臻,李军.采用FISH、DGGE和Cloning对短程脱氮系统中硝化菌群的比较分析[J].环境科学学报,2006,26(5):734-739
采用FISH、DGGE和Cloning对短程脱氮系统中硝化菌群的比较分析
- Analysis of nitrifying bacteria in short-cut nitrification-denitrification processes by using FISH, PCR-DGGE and Cloning
- 基金项目：北京市科技新星计划项目(No.2003A06);国家自然科学基金重大国际合作项目(No.50521140075);北京市重点实验室开放基金
- 作者
- 单位
- 曾薇
- 北京工业大学, 环境与能源工程学院, 北京 100022
- 杨庆
- 北京工业大学, 环境与能源工程学院, 北京 100022
- 张树军
- 北京工业大学, 环境与能源工程学院, 北京 100022
- 马勇
- 北京工业大学, 环境与能源工程学院, 北京 100022
- 刘秀红
- 哈尔滨工业大学, 市政环境工程学院, 哈尔滨 150090
- 彭永臻
- 北京工业大学, 环境与能源工程学院, 北京 100022
- 李军
- 浙江工业大学建筑工程学院, 杭州 310014
- 摘要：针对4种不同的实际污水短程生物脱氮系统(SBR大型中试反应器、UASB-A/O小型反应器、A/O中试反应器和SBR小型反应器),采用Fish、PCR-DGGE和PCR-Cloning-Sequencing分子生物学方法对系统中硝化菌群AOB和NOB进行定性与定量化分析.Fish结果表明,在4种短程脱氮系统中,AOB相比于NOB已成为明显的优势菌群,占总菌群的3%～12%;在SBR中试和小试反应器中没有检测出NOB;A/O中试反应器中存在极少量的Nitrospira(<0.2%),而UASB-A/O小型反应器中存在极少量的Nitrobacteria(<0.2%).PCR-DGGE结果表明SBR中试、A/O和UASB-A/O3种短程脱氮系统中的AOB均以Nitrosomonas-like为主.SBR大型中试反应器中污泥样品的PCR-Cloning-Sequencing结果表明,所有的克隆相似于Nitrosomonas,其中60%以上的克隆相似于Nitrosomonas europaea.
- Abstract：Molecular biology techniques of Fish, PCR-DGGE and PCR-Cloning-Sequencing were used to qualitatively and quantitatively analyze ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in SBR large pilot-scale plant, UASB-A/O lab-scale reactor, A/O pilot-scale plant and SBR lab-scale reactor with the performance of short-cut nitrification-denitrification treating actual wastewater. Fish results showed that in the above 4 processes, AOB became dominant with compared to NOB and accounted for 3%～12% of total biomass. No NOB was detected in both SBR pilot-scale and lab-scale reactors. A very small amount of Nitrospira and Nitrobacteria below 0.2% was found in A/O pilot-scale plant and UASB-A/O lab-scale reactor, respectively. PCR-DGGE results showed that the AOB in SBR pilot-scale, A/O pilot-scale and UASB-A/O lab-scale processes were phylogenetically related to Nitrosomonas-like species with a melting point in the range 30% to 50%. PCR-Cloning-Sequencing results showed that all clones from the sludge sample of the SBR large pilot-scale plant were affiliated with Nitrosomonas and 60% of the clones were affiliated with Nitrosomonas europaea.
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