| 正十六烷微生物降解酶的定域和酶促降解性 |
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| 引用本文： | 陈延君,王红旗,熊 樱.正十六烷微生物降解酶的定域和酶促降解性[J].环境科学研究,2007,20(6):120-125. |
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| 作者姓名： | 陈延君 王红旗 熊 樱 |
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| 作者单位： | 北京师范大学 水科学研究院,水沙科学教育部重点实验室,北京 100875 |
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| 摘 要： | 以正十六烷为研究对象，通过室内实验，利用细胞静息技术提取了2株菌种的胞外酶、膜周酶及膜内酶对石油烃类污染物质的微生物降解酶定域，并研究了菌株受环境影响的产酶条件和酶的一般性质. 结果发现:蜡状芽孢杆菌DQ01能降解正十六烷的关键酶位于膜周和膜内，芽孢杆菌DQ02降解正十六烷的关键酶是胞外酶和膜内酶. 通过GC-MS对代谢产物进行测定发现，关键酶对十六烷的代谢途径是常见的单末端氧化. 2株菌种产酶的最佳环境条件:ρ(正十六烷)为100 mg/L，c(鼠李糖脂)为2 mmol/L. 另外，关键酶在pH为6.5～8.0的环境中活性较高，在pH为7.0左右时的活性最高. 酶促降解性的最适温度为30 ℃.
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| 关 键 词： | 微生物 酶促降解性 正十六烷 静息细胞技术 |
| 文章编号： | 1001-6929（2007）06-0120-06 |
| 收稿时间： | 2007-01-29 |
| 修稿时间： | 2007-05-25 |
Study on Localization of Degradation Enzyme for n-Hexadecane and Enzymatic Degradability |
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| CHEN Yan-jun,WANG Hong-qi and XIONG Ying.Study on Localization of Degradation Enzyme for n-Hexadecane and Enzymatic Degradability[J].Research of Environmental Sciences,2007,20(6):120-125. |
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| Authors: | CHEN Yan-jun WANG Hong-qi and XIONG Ying |
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| Affiliation: | Key Laboratory for Water and Sediment Sciences of Ministry of Education, School of Water Sciences, Beijing Normal University, Beijing 100875, China |
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| Abstract: | Localization of degradation enzyme in two bacteria for n-Hexadecane, as well as production and characteristics of n-Hexadecane-degrading enzyme were studied. The cellular extracts of Bacillus cereus DQ01 were fractionated intoextra-cellular, periplasmic and cytoplasmic fractions after osmotic shock. The results showed that the main enzymes, in the Bacillus cereus DQ01, which are cytoplasmic and membrane-associated enzymes, decomposed mainly n-Hexadecane. And most of the enzyme activities in Bacillus sp. DQ02 were detected in the cytoplasmic and extra-cellular extracts. The detection of metabolites revealed that the periplasmic and cytoplasmic enzymes can degrade n-Hexadecane by the most common pathway. Moreover, culture conditions have a significant effect on the enzyme activityproduced in hexadecane cultures of Bacillus cereus DQ01 and Bacillus sp. DQ02, and the optimal concentration of n-Hexadecane and rhamnolipid is respectively 100 mg/L and 2 mmol/L. The enzyme is active when the pH is in the range of 6.5～8.0, and the most suitable pH is near 7.0. The enzyme is active at temperatures up to 30 ℃. |
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| Keywords: | microorganism enzymatic degradation n-Hexadecane Osmotic shock |
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