Brain modulation of Dufour’s gland ester biosynthesis in vitro in the honeybee (<Emphasis Type="Italic">Apis mellifera</Emphasis>)

Caste-specific pheromone biosynthesis is a prerequisite for reproductive skew in the honeybee. Nonetheless, this process is not hardwired but plastic, in that egg-laying workers produce a queen-like pheromone. Studies with Dufour’s gland pheromone revealed that, in vivo, workers’ gland biosynthesis matches the social status of the worker, i.e., sterile workers showed a worker-like pattern whereas fertile workers showed a queen-like pattern (production of the queen-specific esters). However, when incubated in vitro, the gland spontaneously exhibits the queen-like pattern, irrespective of its original worker type, prompting the notion that ester production in workers is under inhibitory control that is queen-dependent. We tested this hypothesis by exposing queen or worker Dufour’s glands in vitro to brain extracts of queens, queenright (sterile) workers and males. Unexpectedly, worker brain extracts activated the queen-like esters biosynthesis in workers’ Dufour’s gland. This stimulation was gender-specific; queen or worker brains demonstrated a stimulatory activity, but male brains did not. Queen gland could not be further stimulated. Bioassays with heated and filtered extracts indicate that the stimulatory brain factor is below 3,000 Da. We suggest that pheromone production in Dufour’s gland is under dual, negative–positive control. Under queenright conditions, the inhibitor is released and blocks ester biosynthesis, whereas under queenless conditions, the activator is released, activating ester biosynthesis in the gland. This is consistent with the hypothesis that queenright workers are unequivocally recognized as non-fertile, whereas queenless workers try to become “false queens” as part of the reproductive competition.     

Within-population variation in ejaculate characteristics in a prolonged breeder,Peron’s tree frog, <Emphasis Type="Italic">Litoria peronii</Emphasis>

Sperm number is often a good predictor of success in sperm competition; however, it has become increasingly clear that, for some species, variation in probability of paternity cannot be explained by sperm number alone. Intraspecific variation in ejaculate characteristics, such as the number of viable sperm and sperm longevity, may play an equally important role in determining fertilization success. Here, we assess variation among ejaculates in three factors that may contribute to fertilization success (number of sperm per ejaculate, viability, and longevity), in a population of Peron’s tree frog (Litoria peronii). We detected large variation among males in the number of sperm per ejaculate and the proportion of viable sperm within ejaculates, which could not be explained by variation in either male size or body condition. However, the proportion of viable sperm released by males increased over the season. Finally, we assessed sperm longevity (proportion viable sperm determined using a dual-fluorochrome vital dye) at two different temperatures. At 23°C, on average, 75% of sperm remained viable after 2 h, but there were significant differences amongst males with the percentage of viable sperm ranging from 43% to 95%. For sperm incubated at 4°C, ejaculates varied fivefold in sperm longevity with some males having 50% viable sperm after 5 days. Our data suggest that ejaculate characteristics (sperm number, viability, and longevity) vary widely in Peron’s tree frog and may therefore play an important role in determining siring success both in the presence and absence of sperm competition. We discuss the results in relation to selection on ejaculate traits via natural and sexual selection in this and other amphibians.     

Individual protein balance strongly influences δ<Superscript>15</Superscript>N and δ<Superscript>13</Superscript>C values in Nile tilapia,<Emphasis Type="Italic"> Oreochromis niloticus</Emphasis>

Although stable isotope ratios in animals have often been used as indicators of the trophic level and for the back-calculation of diets, few experiments have been done under standardized laboratory conditions to investigate factors influencing ¹⁵N and ¹³C values. An experiment using Nile tilapia [Oreochromis niloticus (L.)] was therefore carried out to test the effect of different dietary protein contents (35.4, 42.3, and 50.9%) on ¹⁵N and ¹³C values of the whole tilapia. The fish were fed the isoenergetic and isolipidic semi-synthetic diets at a relatively low level. ¹⁵N and ¹³C values of the lipid-free body did not differ between the fish fed the diets with different protein contents, but the trophic shift for N and C isotopes decreased with increasing protein accretion in the individual fish, for N from 6.5 to 4 and for C in the lipid-free body from 4 to 2.5. This is the first study showing the strong influence of the individual protein balance to the degree to which the isotopic signature of dietary protein was modified in tissue protein of fish. The extrapolation of the trophic level or the reconstruction of the diet of an animal from stable isotope ratios without knowledge of the individual physiological condition and the feeding rate may lead to erroneous results.     

Urban CO<Subscript>2</Subscript> emissions in Xi’an and Bangalore by commuters: implications for controlling urban transportation carbon dioxide emissions in developing countries

China and India together have more than one third of the world population and are two emerging economic giants of the developing world now experiencing rapid economic growth, urbanization, and motorization. The urban transportation sector is a major source of carbon dioxide (CO₂) emissions in China and India. The goal of this study is to analyze the characteristics and factors of CO₂ emissions produced by commuters in Chinese and Indian cities and thus to identify strategies for reducing transportation CO₂ emissions and mitigating global climate change. Xi’an in China and Bangalore in India were chosen as two case study cities for their representativeness of major cities in China and India. The trends of CO₂ emissions produced by major traffic modes (electric motors, buses, and cars) in major cities of China and India were predicted and analyzed. The spatial distributions of CO₂ emissions produced by commuters in both cities were assessed using spatial analysis module in ArcGIS (Geographic Information System) software. Tobit models were then developed to investigate the impact factors of the emissions. The study has several findings. Firstly, in both cities, the increase of vehicle occupancy could reduce commuting CO₂ emissions by 20 to 50 % or conversely, if vehicle occupancy reduces, an increase by 33.33 to 66.67 %. It is estimated that, with the current increasing speed of CO₂ emissions in Xi’an, the total CO₂ emissions from electric motors, buses, and cars in major cities of China and India will be increased from 135?×?10⁶ t in 2012 to 961?×?10⁶ t in 2030, accounting for 0.37 to 2.67 % of the total global CO₂ emissions of 2013, which is significant for global climate change. Secondly, households and individuals in the outer areas of both cities produce higher emissions than those in the inner areas. Thirdly, the lower emissions in Xi’an are due to the higher density and more compact urban pattern, shorter commuting distances, higher transit shares, and more clean energy vehicles. The more dispersed and extensive urban sprawl and the prevalence of two-wheeler motorbikes (two-wheeler motorbike is abbreviated as “two-wheeler” in the following sections) fueled by gasoline cause higher emissions in Bangalore. Fourthly, car availability, higher household income, living outside the 2nd or Outer Ring Road, distance from the bus stop, and working in the foreign companies in Bangalore are significant and positive factors of commuting CO₂ emissions. Fifthly, “70-20” and “50-20” (this means that generally, 20 % of commuters and households produce 70 % of total emissions in Xi’an and 20 % of commuters and households produce 50 % of total emissions in Bangalore) emission patterns exist in Xi’an and Bangalore, respectively. Several strategies have been proposed to reduce urban CO₂ emissions produced by commuters and further to mitigate global climate change. Firstly, in the early stage of fast urbanization, enough monetary and land investment should be ensured to develop rail transit or rapid bus routes from outer areas to inner areas in the cities to avoid high dependency on cars, thus to implement the transit-oriented development (TOD), which is the key for Chinese and Indian cities to mitigate the impact on global climate change caused by CO₂ emissions. Secondly, in Bangalore, it is necessary to improve public transit service and increase the bus stop coverage combined with car demand controls along the ring roads, in the outer areas, and in the industry areas where Indian foreign companies and the governments are located. Thirdly, Indian should put more efforts to provide alternative cleaner transport modes while China should put more efforts to reduce CO₂ emissions from high emitters.     

海伦&#183;伊丽莎白&#183;克拉克

海伦&#183;伊丽莎白&#183;克拉克（Helen Elizabeth Clark）,1950年2月26日出生于新西兰哈密尔顿市。她早年就读于提帕胡小学、奥克兰爱普松女子语言学校,后在奥克兰大学攻读文学学士和文学硕士学位,于1976年获得大学格兰特委员会奖学金,随后出国留学,之后在奥克兰大学政治系执教8年。     

Females’ sampling strategy to comparatively evaluate prospective mates in the peacock blenny <Emphasis Type="Italic">Salaria pavo</Emphasis>

Emerging evidence suggests the occurrence of comparative decision-making processes in mate choice, questioning the traditional idea of female choice based on rules of absolute preference. In such a scenario, females are expected to use a typical best-of-n sampling strategy, being able to recall previous sampled males based on memory of their quality and location. Accordingly, the quality of preferred mate is expected to be unrelated to both the number and the sequence of female visits. We found support for these predictions in the peacock blenny, Salaria pavo, a fish where females have the opportunity to evaluate the attractiveness of many males in a short time period and in a restricted spatial range. Indeed, even considering the variability in preference among females, most of them returned to previous sampled males for further evaluations; thus, the preferred male did not represent the last one in the sequence of visited males. Moreover, there was no relationship between the attractiveness of the preferred male and the number of further visits assigned to the other males. Our results suggest the occurrence of a best-of-n mate sampling strategy in the peacock blenny.     

Does exaggerated morphology preclude plasticity to cuckoldry in the midshipman fish (<Emphasis Type="Italic">Porichthys notatus</Emphasis>)?

In species with more than one male reproductive morph, there typically exists a larger morph with exaggerated secondary sexual characters, and a smaller morph with reduced secondary sexual characters. These exaggerated and reduced morphologies are commonly thought to represent specializations to alternative behavioral reproductive tactics—large body size and exaggerated secondary sexual characters should both facilitate territoriality, courtship, and pair-spawning; while small body size and reduced secondary sexual characters should facilitate sneaky cuckoldry. Given this postulated relationship between morphology and behavior, we examined the relationship between the morphology of exaggerated males and cuckoldry. In a field and aquarium study of the midshipman fish, a fish with both exaggerated and reduced morphs, we demonstrated cuckoldry in some males of the exaggerated morph. Since the reduced morphology is thought to be an adaptation towards sneaky cuckoldry, we predicted that, of males with the exaggerated morph, less-exaggerated (smaller) males would be better able to gain proximity to the spawning pair during cuckoldry. In contrast to that prediction, access to the spawning pair during cuckoldry increased with the body size of the cuckolding exaggerated-morph males. This may be related to our observation that exaggerated males often cuckolded aggressively. Thus the exaggerated morphology need not preclude adaptive plasticity to cuckoldry, and may even aid it.     

胡利娅&#183;卡拉维亚斯&#183;利略

胡利娅&#183;卡拉维亚斯&#183;利略。墨西哥前环境、资源和渔业部部长,墨西哥国立自治大学生物学教授。卡拉维亚斯教授1954年出生于墨西哥城,在墨西哥国立自治大学获得学位后留校教授环境科学并从事热带雨林保护、自然资源管理和环境保护方面的研究。     

Exchange of hosts: can agaonid fig wasps reproduce successfully in the figs of non-host <Emphasis Type="BoldItalic">Ficus</Emphasis>?

In the obligate mutualism between figs (Ficus) and their specific pollinators (Chalcidoidea, Agaonidae), each species of fig wasp typically reproduces in figs of a single host species. Host specificity is maintained largely because pollinators are attracted to tree-specific volatiles released from their host figs, but whether the wasps can reproduce if they enter figs of non-host species is unclear. We investigated the reproductive success of Ceratosolen emarginatus (associated with Ficus auriculata) and Ceratosolen sp. (associated with F. hainanensis) in atypical hosts by experimentally introducing foundresses into host and non-host figs. F. auriculata figs entered by Ceratosolen sp. were more likely to abort than if entered by C. emarginatus, but abortion of F. hainanensis figs was not affected by pollinator species. Single C. emarginatus foundresses produced more but smaller offspring in F. hainanensis than in their normal host. Conversely Ceratosolen sp. produced fewer but larger offspring in F. auriculata than in their normal host, probably as a result of having longer to develop. Mean style length differences, relative to the lengths of the wasps’ ovipositors, may have dictated the number of offspring produced, with oviposition made easier by the shorter styles in F. hainanensis figs. Our results imply that, in addition to morphological constraints and tree-specific volatiles, reduced reproductive success in atypical hosts can be another factor maintaining host specificity, but for other species only behavioural changes are required for host switching to occur.     

Why do <Emphasis Type="Italic">Manduca sexta</Emphasis> feed from white flowers? Innate and learnt colour preferences in a hawkmoth

Flower colour is an important signal used by flowering plants to attract pollinators. Many anthophilous insects have an innate colour preference that is displayed during their first foraging bouts and which could help them locate their first nectar reward. Nevertheless, learning capabilities allow insects to switch their colour preferences with experience and thus, to track variation in floral nectar availability. Manduca sexta, a crepuscular hawkmoth widely studied as a model system for sensory physiology and behaviour, visits mostly white, night-blooming flowers lacking UV reflectance throughout its range in the Americas. Nevertheless, the spectral sensitivity of the feeding behaviour of naïve moths shows a narrow peak around 450 nm wavelengths, suggesting an innate preference for the colour blue. Under more natural conditions (i.e. broader wavelength reflectance) than in previous studies, we used dual choice experiments with blue- and white-coloured feeders to investigate the innate preference of naïve moths and trained different groups to each colour to evaluate their learning capabilities. We confirmed the innate preference of M. sexta for blue and found that these moths were able to switch colour preferences after training experience. These results unequivocally demonstrate that M. sexta moths innately prefer blue when presented against white flower models and offer novel experimental evidence supporting the hypothesis that learning capabilities could be involved in their foraging preferences, including their widely observed attraction to white flowers in nature.     

Small bite,large impact–saliva and salivary molecules in the medicinal leech, <Emphasis Type="Italic">Hirudo medicinalis</Emphasis>

Blood-sucking leeches have been used for medical purposes in humans for hundreds of years. Accordingly, one of the most prominent species has been named Hirudo medicinalis by Carl Linne in 1758. Feeding on vertebrate blood poses some serious problems to blood-sucking ectoparasites, as they have to penetrate the body surface of the host and to suppress the normal reactions of the host to such injuries (swelling, pain, inflammation) to remain undetected during the feeding period. Furthermore, the parasites have to take measures to inhibit the normal reactions in host tissues to blood vessel damage, namely hemostasis and blood coagulation (platelet aggregation and activation, activation of thrombin and formation of fibrin clots). During evolution, leeches have acquired the ability to control these processes in their hosts by transferring various bioactive substances to the host. These substances are supposedly produced in unicellular salivary gland cells and injected into the wound at the feeding site through tiny salivary ductule openings in the jaws that the leech uses to slice open the host body surface and to cut blood vessels in the depth of the wound. This review summarizes current knowledge about the salivary gland cells and the biological effects of individual saliva components as well as hints to the potential usefulness of some of these compounds for medical purposes.     

A male gift to its partner? Cyanogenic glycosides in the spermatophore of longwing butterflies (<Emphasis Type="Italic">Heliconius</Emphasis>)

Males of several insect species transfer nuptial gifts to females during mating, typically in the form of a protein-rich spermatophore. In chemically defended species, males could potentially enhance such a gift with chemicals that help protect the female, her eggs, or both. This was shown for lepidopteran species that accumulate pyrrolizidine alkaloids. Most Heliconius butterflies are presumably protected from predators by virtue of de novo synthesized and/or sequestered cyanogenic glycosides. Males of Heliconius species are known to transfer nutritional gifts to the females but whether defensive chemicals could also be transferred is not known. To ascertain whether transfer of cyanogens occurs, we dissected freshly mated females from nine different Heliconius species and analyzed spermatophores for cyanogenic glycosides. We found cyanogens in the spermatophores of all nine species. This is the first time cyanogenic glycosides are reported in the spermatophores of arthropods. We discuss the implications of these findings for Heliconius biology and for other cyanogenic insects as well. We suggest that chemically defended species commonly lace their nuptial gifts with defensive chemicals to improve gift quality.     

让废电池"回家"

河南新乡的个体户田桂荣从1999年开始发起了回收废电池的活动,几百个废电池回收箱,几万份传单,随着坚持不懈的努力和人们环保意识的增强,她得到了越来越多的理解和和支持,废电池回收量日渐增长.然而,麻烦也来了:"这么多废电池到底咋办?真是难死我了!谁能告诉我处理废电池的好办法?"她这样说. 其实受此网扰的绝不只是田桂荣,废电池的回收利用一直是个难题.废电池有毒,不能乱扔,这大家都知道.于是很多学校、机关、商店都开展了废电池回收活动.但没过多久,各地的活动就陷入困境,因为收上来的废电池找不到出路,堆积如山,反成了负担……     

比尔&#183;金

比尔&#183;金先生（通常大家叫他Bill）现在是TNC（大自然保护协会）副总裁、全球首席生态保护执行官,统管TNC的全球保护事务（下设9大部门,涉及32个国家）,并负责推动机构最重要的保护项目和保护策略。比尔&#183;金在TNC任职已经超过12年,曾在机构内担任过多个重要的领导职务。     

斯塔夫罗&#183;迪马斯

斯塔夫罗&#183;迪马斯，欧盟环境专员，生于1941年4月，早年就读于雅典大学并取得法律与经济学学士学位，后在美国纽约大学取得法律硕士学位。1977年至2004年间，他曾作为新民主党代表连续十次被选为国会议员并且在希腊政坛处于领导地位，先后出任贸易部长、新民主党国会发言人、农业部长、工业能源及科技部长和新民主党秘书长等重要职位。     

洗涤之"伤"

毫无疑问，洗涤剂为个人保健和家居保洁提供了帮助，成为许多家庭的必备品。但这些洗涤剂潜在的化学危害却常常被人忽略。     

自然·水·人

人类社会向前发展,人向自然界的索取不断增加,对自然的干扰也逐步加剧,致使自然界的承受能力受到影响,人与自然的关系出现紧张。为此,人必须处理好与自然的关系,以免人的生存环境受到威胁。水是自然界的重要因素,和人的生存与人类社会的发展关系密切。由于用水量不断增加和用水不当,一些地区出现水资源危机,人在和水旱灾害进行抗争中,也出现了由于措施不当带来的人为加大灾害的问题。因此,人必须在处理好与自然关系的框架中,认真处理好与水的关系,以保障水资源的永续利用和人类社会的可持续发展。     

Developing long-term strategies to reduce energy use and CO<Subscript>2</Subscript> emissions—analysis of three mitigation scenarios for iron and steel production in China

We perform a scenario analysis of three strategies for long-term energy savings and carbon dioxide (CO₂) emission reductions in iron and steel production in China, using a linear optimization modeling framework industry sector energy efficiency modeling (ISEEM). The modeling includes annual projections for one base scenario representing business-as-usual (BAU) and three additional scenarios representing different strategies to reduce annual energy use and CO₂ emissions from 2010 to 2050. Specifically, the three scenarios for cost-optimization modeling include changing the production share (PS), predefining emission reduction (ER) target, and stipulating carbon emission pricing (CP), respectively. While the three strategies are projected to result in similar annual energy savings by approximately 15 % compared to that of the BAU scenario in year 2050, the carbon emission pricing strategy brings about the highest annual energy savings in the medium term (e.g., 2025). In addition, adopting carbon emission pricing strategy will result in the highest emission reduction from BAU with much higher costs, i.e., by 20 % in 2025 and 41 % in 2050, while adopting either PS or ER strategies will result in a moderate level of emission reduction from BAU, i.e., by approximately 4 % in 2025 and 14 % in 2050. The analysis of China’s national strategies to reduce energy use and emissions provides important implications for global mitigation strategies.     

Prospective thinking in a mustelid? <Emphasis Type="Italic">Eira barbara</Emphasis> (Carnivora) cache unripe fruits to consume them once ripened

The ability of nonhuman animals to project individual actions into the future is a hotly debated topic. We describe the caching behaviour of tayras (Eira barbara) based on direct observations in the field, pictures from camera traps and radio telemetry, providing evidence that these mustelids pick and cache unripe fruit for future consumption. This is the first reported case of harvesting of unripe fruits by a nonhuman animal. Ripe fruits are readily taken by a variety of animals, and tayras might benefit by securing a food source before strong competition takes place. Unripe climacteric fruits need to be harvested when mature to ensure that they continue their ripening process, and tayras accurately choose mature stages of these fruits for caching. Tayras cache both native (sapote) and non-native (plantain) fruits that differ in morphology and developmental timeframes, showing sophisticated cognitive ability that might involve highly developed learning abilities and/or prospective thinking.