Pollution correlated modifications of liver antioxidant systems and histopathology of fish (Cyprinidae) living in Seyhan Dam Lake, Turkey

Specimen of freshwater fish (Cyprinidae) was collected from polluted and clean areas in dam Lake of Seyhan. Ten (n=10) fish were taken from each site. Liver preparations of fish from the two sources were comparatively assayed for glutathione-S-transferase (GST), lactic dehydrogenase (LDH), catalase (CAT), glucose-6-phosphate dehydrogenase (G6PDH), superoxide dismutase (SOD) and malondialdehyde (MDA). All systems were significantly (p<0.1) affected at the polluted site. The microscopic examination of the liver tissues of the fish living in the polluted area showed mononuclear inflammatory cell infiltration, cloudy degenerations, congestions and also micro- and macrovesicular fatty degenerations. These results provide evidence that both biochemical parameters and histopathological observations can be assumed as sensitive indicators of fish liver metabolism for polluted areas.     

Species Specificity of the Cytogenetic Stability of Fish in a Eutrophic Water Body

The micronucleus test of peripheral blood erythrocytes was used to study cytogenetic stability in bream (Abramis brama L.), perch (Perca fluviatilis L.), roach (Rutilus rutilus L.), and pike (Esox lucius L.) from a eutrophic water body not affected by anthropogenic factors. The dependence of the rate of spontaneous micronucleus formation on fish age was estimated. The effect of natural variation in abiotic environmental factors on the cytogenetic stability of species was analyzed.     

Copper and zinc in four freshwater fish species from Lake Pamvotis (Greece)

Lake ecosystems are vulnerable to heavy-metal pollution. Fish samples are considered as one of the most indicative factors, in freshwater systems, for the estimation of trace metals pollution potential. Lake Pamvotis (NW Greece) is a typical Mediterranean ecosystem of great importance in regard to biodiversity and to aesthetic value. The fish species found most commonly in the lake are Cyprinus carpio, Silurus aristotelis, Rutilus ylikiensis, and Carassius gibelio. The aim of the present study is to evaluate the level of contamination of two essential heavy metals (copper and zinc) appearing at high concentrations in lake water in the above four fish species. Metal concentrations were measured by atomic absorption spectroscopy in three different tissues (muscle, liver, gonads) in order to assess the fish contamination. A two-factor analysis of variance, based on the procedure of general linear models, was employed in which fish species (four levels) and fish tissue (three levels) were examined for potential influence on Cu and Zn concentrations. Differences between level means per factor were treated using Tukey's multiple comparisons of means. The study showed that C. carpio and R. ylikiensis presented the highest metal content. Tissues analysis revealed that liver and gonads accumulated the highest levels of Cu and Zn. Metal concentration in the edible part of the examined fish (muscle) were in the safety-permissible levels for human consumption.     

Faunal diversity and dynamics of species richness and dominance of parasite communities in age series of the perch (<Emphasis Type="Italic">Perca fluviatilis</Emphasis>)

Changes in parameters of species richness and dominance in parasite communities of perch have been analyzed in the age series of hosts from two water bodies, Lake Gusinoe and Chivyrkui Bay (Lake Baikal), at the levels of a host individual (infracommunity) and a host age group (set of infracommunities). The species richness of parasite infracommunities has been found to increase in both age series due to decreasing proportions of fish affected by no more than three to four parasite species and increasing proportion of fish parasitized by more than four to five species.     

Effect of anthropogenic pollution on oxidative processes in the liver of fish from the Rybinsk Reservoir

The levels of total lipids, peroxidation products, and antioxidant activity in the liver were studied in fish species living in unequally polluted areas of the Rybinsk Reservoir and differing in their ecology and taxonomic position: the bream (Abramis brama L.), roach (Rutilus rutilus L.), burbot (Lota lota L.), and zander (Stizostedion lucioperca L.). The fish from polluted water areas were characterized by higher levels of total lipids and malonic dialdehyde and a deficiency of antioxidants. It is shown that the test parameters depend on the type of feeding and mode of life of the fish species.     

太湖水域鮈亚科鱼类的时空分布

为了研究太湖水域鮈亚科鱼类的时空分布特征,对2013年度太湖水域8个监测点的渔获物进行统计分析,进一步推测太湖水域鱼类种群结构的变化趋势。结果表明太湖水域鮈亚科鱼类主要分布于近岸区的平台山、贡湖及鲤山湾等监测水域。麦穗鱼、花、棒花鱼及黑鳍鳈为太湖水域鮈亚科鱼类的优势种群,8个监测位点鮈亚科鱼类的渔获数量与总渔获量的比例介于1.84%~27.7%。以渔获数量为基础获得的鮈亚科鱼类多样性指数H′在0.1679~1.6860之间,且太湖水域的鱼类优势种群逐渐趋于单一化、小型化,太湖水域的生态环境演化和过渡捕捞是形成这一趋势的主要原因。 关键词： 太湖;鮈亚科鱼类;时空分布;多样性指数     

Perfluorinated compounds in common carp (Cyprinus carpio) fillets from the Upper Mississippi River

Ten different perfluoroalkyl acids (PFAAs), including perfluooctane sulfonate (PFOS), were measured in 30 common carp (Cyprinus carpio) fillets collected from three sites on the Upper Mississippi River in Minnesota in an effort to evaluate the potential impact of PFAA emissions in this area. Samples upstream of the city of St. Cloud (reference site) had median PFOS concentrations of 8.1 ng/g wet weight (ng/g wet wt), but median levels increased significantly downstream in the Minneapolis-St. Paul urban area, with concentrations from the Pig's Eye Lake site at 26 ng/g wet wt (p=0.0015) and the Spring Lake site at 40 ng/g wet wt (p=0.0004). This latter PFOS concentration is within the advisory range for limiting fish consumption to one meal a week according to the Minnesota Department of Health. Other PFAAs were also found to increase significantly between the reference site and the Minneapolis-St. Paul area, but maximal concentrations remained below 2.0 ng/g wet wt. This study demonstrates the bioaccumulation of PFAAs in a ubiquitous fish species in a major urban area known to have historical inputs of various PFAA compounds. The full extent of this contamination and the potential for accumulation in other species remain to be evaluated.     

Levels of organochlorine pesticides, polychlorinated biphenyls and polybrominated diphenyl ethers in fish species from Kahramanmaras, Turkey

The levels of organohalogenated contaminants, such as organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ethers (PBDEs) were measured in four fish species (Acanthobrama marmid (kalashpa), Cyprinus carpio (carp), Chondrostoma regium (nose-carp), and Silurus glanis (wels)) from the Sir Dam Lake, Kahramanmaras, Turkey. These species were selected for their characteristic feeding behaviour and their importance to local human fish consumption. DDTs were the predominant organohalogenated contaminants in all species, with the p,p'-DDE contributing to more than 90% to the total DDTs. Other OCPs, such as hexachlorocyclohexane (HCH) isomers, chlordanes and hexachlorobenzene (HCB) were found at much lower levels in all five species. The levels of PCBs and PBDEs (on wet weight basis) were lower than in similar species from European or American freshwater systems. PBDE data were measured for the first time in fish species from Turkish environment. Lipid-based concentrations of OCPs, PCBs and PBDEs were higher in wels than in the other species and this was related to its piscivorous feeding mode and to its higher lipid content. Contrarily, concentrations of pollutants in nose-carp were the lowest, in agreement with its more herbivorous diet. A preferential accumulation in muscle compared to liver was observed for all OCPs, PCBs, and PBDEs in wels and carp, while in nose-carp, a preferential accumulation in liver was observed only for PBDEs, p,p'-DDT and PCBs. Racemic amounts for alpha-HCH were measured in all investigated muscle and liver samples, except for carp muscle.     

Fly ash leachate induces oxidative stress in freshwater fish Channa punctata (Bloch)

Oxidative stress inducing potential of fly ash leachate (FAL) was studied in a freshwater fish, Channa punctata (Bloch). Fish were exposed to fly ash leachate for 24 h and lipid peroxidation (LPO) was studied as a marker of oxidative stress. Catalase (CAT), glutathione S-transferase (GST) activities and levels of reduced glutathione (GSH) were also estimated in the exposed fish. FAL (1 ml/l) induced LPO in all the organs and most prominent response was in the gill. It also caused induction of enzymes and glutathione. Liver showed highest level of induction of enzyme activities. The results of this study demonstrate that fly ash constituents have potential to induce oxidative stress in fish and gills are the most vulnerable organs. It is also suggested that in case of exposure to FAL, along with LPO antioxidant defense is also activated to counteract the reactive oxygen species (ROS) at least partly in the initial stages of exposure.     

Intrapopulation spatial segregation in bream (<Emphasis Type="Italic">Abramis brama</Emphasis>) and roach (<Emphasis Type="Italic">Rutilus rutilus</Emphasis>) in the Rybinsk Reservoir

In the course of studies on mass fish species (bream and roach) in water bodies of the Upper Volga basin, intraspecific groups of individuals have been distinguished. Conditions and possible mechanisms of spatial differentiation of these groups are considered. The intrapopulation divergence of individuals by some adaptive characters, primarily behavioral, enables them to utilize alternative resources, which provides for more efficient use of the environment by populations.     

Heavy metals in fish (Solea vulgaris, Anguilla anguilla and Liza aurata) from salt marshes on the southern Atlantic coast of Spain

Comparisons were made of metal concentrations in water and sediment with those in the muscle and livers of three species of fish caught in four seawater reservoirs (two in the Odiel estuary and two in the Bay of Cádiz). The concentrations of a large number of metals in the water, sediment and fish were higher in the Odiel reservoirs than in those in Cádiz. We found high metal-enrichment factors in the livers as compared to muscle (over 100 for Fe and Cu and around 5 for Zn, Ni, Cd and Pb). The three fish species showed differences in metal content. For example, eels accumulated more metals in muscle and grey mullet in the liver. The metal levels found in muscle in the three species were below the legal limits for human consumption, although Cu in the liver was above the limit in the three species taken from the Odiel reservoirs, and Zn levels in liver were over the maximum in eels from one of the Odiel marshes. Significant correlations (p<0.05) were obtained for the levels of numerous metals in water, sediment and fish.     

长湖鱼类群落结构的时空变化

为系统阐明长湖鱼类群落结构特征,2014年3~11月对长湖鱼类群落结构的空间差异和季节动态进行了较为全面的调查。结果表明,长湖共调查到鱼类43种,隶属于7目14科37属,主要为湖泊定居型鱼类;大湖区和海子湖区鱼类优势种组成虽存在一定的差异,但鲫、鰐、鲤和达氏鲌为两个湖区的共同优势种;长湖鱼类群落的Margalef丰富度指数、Shannon-Wiener多样性指数和Pielou均匀度指数分别为0.53~2.30、0.52~2.02和0.24~0.66,除丰富度指数外,均表现为5月或8月相对较高;聚类和MDS排序分析结果表明长湖鱼类群落分为两大类群,呈现显著的时空异质性;ABC曲线显示长湖优势鱼类以小型鱼类或大型鱼类的幼体为主,且已受到不同程度的干扰。针对长湖鱼类资源已出现衰退,建议在拆除围网/拦网养殖、取缔有害渔具的同时,考虑增殖放流、栽种水生植物等措施来达到长湖鱼类资源保护和可持续利用的目的。     

鄱阳湖子湖“堑秋湖”渔业资源结构特征分析

"堑秋湖"是广泛存在于鄱阳湖湖区且与鄱阳湖水文节律特征相顺应的一种渔业方式。为了解"堑秋湖"渔获物组成特征及其产量与水位之间的关系,于2011~2013年"堑秋湖"期间对江西鄱阳湖国家级自然保护区的子湖大湖池和沙湖进行了连续3年的渔业调查。2011年,大湖池和沙湖与修河分别相通23d和10d,鄱阳湖属旱涝急转气候;2012年鄱阳湖水势较大,大湖池和沙湖与修河分别相通124d和108d。2013年鄱阳湖再现旱情,大湖池和沙湖均未能与修河连通。2011年渔业调查开展7次,2012年调查9次,2013年调查5次。3a调查累计获得鱼类59种隶属于6目12科43属。3年研究结果表明渔获物以鲤科鱼类为主,在种类上变化不大但优势物种有所改变且渔获物产量呈下降趋势,无度量多维排序结果(MSD)显示鱼类群落在不同年度和子湖间分离明显。经济鱼类的单位捕捞努力量(CPUE)与水位变化的线性回归分析结果表明,2011年经济鱼类产量与当日水位呈正相关性,但2012年和2013年两者的相关性却不明显。     

Influence of fish size and sex on mercury/PCB concentration: importance for fish consumption advisories

Fish advisories for polychlorinated biphenyls (total-PCBs) and mercury are often given on a length-specific basis and fish sex is usually not considered. The relationship between concentration and length is well established for mercury, however its suitability for total-PCBs and the influence of sex over the large scale covered by most monitoring programs is not well known. Here we use what is perhaps the largest consistent sport fish contaminant dataset to evaluate the relationship between total-PCB/mercury and length and sex. We evaluated seven of the most commonly consumed fishes from the Canadian Great Lakes and two Ontario (Canada) inland lakes. For mercury, the relationship between concentration and length was significant (p<0.05) in most fish species and locations. For total-PCB, this relationship was also generally significant in Chinook salmon and lake trout, which are the species with the most advisories for this compound. In contrast, significant relationships were found less often for whitefish, northern pike, smallmouth bass, walleye, and especially yellow perch. However, mercury usually drives fish consumption advisories for these species. Overall, our results support the protocol of issuing contaminant advice on a length-specific basis in fish monitoring programs with reasonable confidence when at least a moderate number of samples over the natural size range of a species are included in the analysis. Sex differences were common for walleye (males>females, p<0.05) but not other species, suggesting that an equal number of male and female walleye should be used in deriving fish advisories for walleye.     

PCDD/Fs and DL-PCBs intake from fish caught in Polish fishing grounds in the Baltic Sea — Characterizing the risk for consumers

Fish and fishery products are among the primary sources of dietary exposure to dioxins. It is known that some fish species caught in the Baltic Sea contain elevated level of those compounds. Levels of dioxins and DL-PCBs in 236 Baltic fish samples (including 65 salmon, 14 sea trout, 63 sprat, 63 herring, 31 cod), and 20 cod liver samples from the Polish fishing grounds (the ICES zones 24–27), collected in the time frame of 2006–2011 as part of Polish monitoring survey have been used for risk assessment. To characterize potential health risk associated with dioxins intake, doses ingested in a single portion of fish and cod liver by adults (200 g for fish, 125 g for cod liver), and children (100 g for fish, 25 g for cod liver) were expressed as percent of Tolerable Weekly Intake (TWI) and Provisional Tolerable Monthly Intake (PTMI).Average dioxins intake estimated for fatty fish species was about 250% TWI for children, and about 170% TWI for adults, with maximum values of 436.3 and 288.0% TWI, respectively. Maximum exposure expressed as percent of PTMI was below 90% for children and below 60% in adults. For sprat and herring, mean dioxins intakes were lower, but still not at “safe” level: 100–150% TWI for children and about 70–100% for adults, with the maximum values of about 250 and 180%, respectively. Maximum exposure expressed as percent of PTMI was approximately 50% for children and 35% for adults.Intakes values calculated for practically “dioxin-free” cod are just theoretical because in calculating toxic equivalents (TEQs) an upperbound approach was applied, and vast majority of TEQs originates from the limit of quantification (LOQ) values of all non-quantified congeners.Frequent consumption of cod liver seems to be a health risk as, according to assumed scenario, dioxins intake of 100% PTMI for adults would be achieved by the 65th percentile, while for children by approximately 90th percentile of results.Serving sizes of salmonids, cod liver, and even sprat, and herring that lead to total dioxins intake equal to TWI, and PTMI were relatively small. Thus, one can easily exceed those toxicological reference values consuming above Baltic fish species available on the Polish market.Taking into account low fish consumption in Poland and, additionally, low share of Baltic fish in total consumption of marine fish, potential risk of high dioxins intake does not apply to general population. Occasionally elevated dioxins intake above TWI or PTMI is not necessarily related to health risk, because of uncertainty factors embedded in these toxicological reference values. However, some sub-populations in Poland that habitually consume fatty fish originating from the Baltic Sea or Baltic cod liver may be at an elevated health risk of potential consequences of chronic exposure to dioxins. Dietary recommendations based on risk-benefit analysis for consumers of such fish and fishery products from the Baltic Sea would be the most effective tool for risk management.     

三峡库区小江鱼类群落特征及其历史变化

为了解三峡库区蓄水后小江流域鱼类群落现状及其历史变化,于2016年春季(5月)、秋季(9月)和冬季(12月)开展了小江鱼类调查。共调查到鱼类37种,隶属于6目11科;种类组成以鲤形目为主,占总数的64.86%,其中黑尾■Hemiculer tchangi和岩原鲤Procypris rabaudi为长江上游特有种类;草鱼Ctenopharyngodon idellus、赤眼鳟Squaliobarbus curriculus、■Elopichthys bambusa、鲫Carassius auratus、鲤Cyprinus carpio、鲢Hypophthalmichthys molitrix、鳙Aristichthys nobilis和黑尾■构成小江主要经济鱼类。对比历史资料分析显示:两次调查结果相似性指数为0.45(为中等不相似);特有种鱼类退化明显:主要经济鱼类组成发生了改变;土著鱼类物种多样性和资源量均呈明显下降趋势,而适应于静水或缓流水环境的鱼类呈上升趋势。推测水坝建设、过度捕捞及不合理的采砂活动等因素是导致变化的主要原因。结果建议,合理设置水坝(汉丰湖)调水机制,对于保护该水域土著鱼类资源具有重要意义。     

Heavy metals in mullet, Liza abu, and catfish, Silurus triostegus, from the Atatürk Dam Lake (Euphrates), Turkey

The distribution of some heavy metals in three different organs of mullet, Liza abu, and catfish, Silurus triostegus, from Atatürk Dam Lake located on Euphrates (Turkey) was studied. Co and Mo concentrations were below limits of detection in all fish organs, whereas Ni was also below limits in organs of mullet. The metal accumulation in the liver and gill of L. abu and S. triostegus was found to be quite high in comparison with the muscle. In general, the concentrations are similar to those previously observed on other fish studied in Atatürk Dam Lake and lower than those determined in Tigris River. The analysed metals (Co, Cu, Fe, Mn, Mo, Ni and Zn) were found in fish muscle at mean concentrations under the permissible limits proposed by FAO.     

西洞庭湖鱼类物种多样性及其时空变化

洞庭湖是长江中游现存仅有的两大通江湖泊之一,研究其鱼类资源对了解湖泊通江的生态作用有着重要意义。为此,在2002年9月~2004年6月每月定期定点在西洞庭湖区开展鱼类调查,结果如下：① 实地调查到鱼类111种,隶属9目20科,包括中华鲟、胭脂鱼等珍稀物种;②鱼类物种数年动态波动较大,最高月份达69种,最低为26种,月均50±13种;③物种多样性指数年动态与物种数年动态大体一致,最高达3.34,最低为1.89,月均2.84±0.38;④空间分布上,位于入湖口的调查点物种数和多样性指数高于内湖,且差异有显著性(p<0.05);⑤在数量上,湖泊定居型鱼类占50%以上,其中,鲫数量最多,占16%左右。可见：西洞庭湖作为通江湖泊在鱼类多样性的保护上起到避难所和交流场所的作用;目前,西洞庭湖鱼类在生态组成上,湖泊定居型鱼类比重有逐渐增加的趋势。     

肖四海不同渔业发展阶段的水环境特征分析

2006～2008年,对长江中游的一个浅水湖泊--肖四海进行了水质和水生植被研究,并结合历史资料分析了肖四海1987～2008年不同渔业发展阶段的水环境特征。研究结果表明：1987～2008年期间,该湖营养状况经历了中营养→富营养→中营养→富营养→中营养的变化过程,沉水植被经历了消亡→恢复→旺盛→衰退的演替过程,这种变化主要受不同阶段的渔业方式的影响。草食性鱼类和河蟹的过量放养、化肥的大量使用以及饵料的大量投放等不合理的渔业方式导致了水生植被的消亡和水体的富营养化,而合理的渔业方式不会对水体环境产生消极影响,并据此提出了长江中下游湖泊渔业可持续发展的建议     

江湖阻隔对涨渡湖区鱼类资源的生态影响

长江中游两岸绝大多数通江湖泊自20世纪50年代以来因水利工程阻隔先后失去与长江的联系而产生一系列的生态问题。我们以涨渡湖为例开展江湖阻隔后对单个湖泊鱼类资源所产生的生态影响进行研究,结果表明：江湖阻隔导致湖泊生产经营方式改变,使得野生渔业产量的比例由1949年的95%,降低到2002年的不足5%;江湖阻隔后湖泊鱼类种类由50年代约80种下降到现在的52种,其中,洄游性和流水型鱼类比重由50%下降至不足30%;迷魂阵渔获物在单位产量、个体大小上极显著(p < 0.01)地高于通江湖泊,但在物种数和多样性指数上则要低得多(p < 0.01)。因此,江湖阻隔对湖泊渔业具明显影响,且正导致野生鱼类资源的进一步衰退。