Mate quality,not aggressive spillover,explains sexual cannibalism in a size-dimorphic spider

Sexual cannibalism particularly before mating is costly for the male victim but also for the female aggressor if she risks remaining unmated. The aggressive spillover hypothesis explains the persistence of this behavior as a maladaptive side effect of positive selection on aggressiveness in a foraging context. The hypothesis predicts that the occurrence of sexual cannibalism is explained by female aggressiveness but is not related to male phenotype or behavioral type. An alternative hypothesis invokes sexual selection and makes the opposite prediction namely that sexual cannibalism is an expression of female choice and should hence mainly target males of low quality. We tested the above hypotheses on a sexually dimorphic nephilid spider Nephilengys livida, known for male monopolization of females via genital damage, female genital plugging, and mate guarding, by staging mating trials during which we recorded mating behaviors and occurrences of pre- and postcopulatory cannibalism. We did not restrict assessment of aggressiveness to the mating and foraging context but also included aggression against same sex conspecifics. To assess female personalities, i.e., consistent individual differences in behavior including aggressiveness, we repeatedly tested them for intra-sex aggression, voracity towards prey, locomotory activity, and boldness. Females exhibited consistent differences in intra-sex aggressiveness, latency to attack prey, and boldness. Aggressive females had shorter latencies to attack prey and were more active than non-aggressive ones. In contrast to the predictions of the aggressive spillover hypothesis, females that were aggressive towards prey and towards other females were not more likely to attack a male than non-aggressive females. In support of the mate choice hypothesis, less aggressive males were more likely attacked and cannibalized than more aggressive ones. This hints at sexual selection for aggressiveness in males and raises the question of mechanisms that maintain variation in male aggressiveness.     

Cheetah mothers' vigilance: looking out for prey or for predators?

Summary Free-living cheetah (Acinonyx jubatus) cubs are killed by a number of predators, thus vigilance in cheetah mothers may be a form of anti-predator behaviour as well as a means of locating prey. Mothers' vigilance during the day was closely associated with measures of hunting but not with measures of anti-predator behaviour. In contrast, mothers' vigilance at kills was not related to hunting but was related to anti-predator behaviour. Both forms of vigilance decreased as cubs grew older. Vigilance during the day increased with litter size which supports a model of shared parental investment (Lazarus and Inglis 1986) because after prey had been located and caught by mothers, cubs shared the prey between them. Vigilance at kills did not increase with litter size when cubs were young; in these situations predators stole cheetahs' prey and rarely chased cubs so, at most, only a single cub would be taken. Mothers' anti-predator behaviour away from kills did increase with litter size at young cub ages however; more cubs are killed in these circumstances the greater is the size of the litter. When cubs were older and could outrun predators, neither vigilance at kills nor anti-predator behaviour increased with litter size. These results strongly support two models of unshared investment (Lazarus and Inglis 1986) and demonstrate, not only that superficially similar behaviour has different functions in different contexts, but that parental investment is shaped by the type of benefits accrued from it.     

An advantage to mate choice in the seaweed fly,Coelopa frigida

Summary All natural populations studied of the seaweed fly, Coelopa frigida, are polymorphic for the inversion system on chromosome I. It has previously been shown that heterokaryotypes have superior egg-to-adult survival than either homokaryotype, and that there is non-random mating with respect to inversion genotypes. We have inquired whether the production of fitter progeny in larger numbers is the possible reason for the existence of non-random mating behaviour. We measured the fertility, fecundity and progeny survival of adults that have either been allowed to exercise mate preference, or been given a single, randomly chosen, mate. The fertility (% of females producing progeny) and progeny survival (% of larvae surviving to the late 3rd instar stage) are both significantly greater in choice than nochoice females. There are also significant differences in fertility and progeny survival depending on whether the parents were of similar or dissimilar inversion genotypes. An effect of relative adult size on fertility was also observed. There appear to be no effects of mate choice on fecundity. Indirect evidence is presented that adults are choosing their mates not only on the basis of genotypes but on other criteria as well. It is suggested that the superior reproductive performance of animals allowed a choice of mates may account for the evolutionary origin and continued existence of mate discrimination. Furthermore, since over 25% of the genome of Coelopa is located within inversions, the observed pattern of negative assortative mating may maintain a substantial fraction of the genes in a polymorphic state.     

Optimal foraging: The influence of intraspecific competition on diet selection

Summary Three-spined sticklebacks (Gasterosteus aculeatus) showed a relative preference for a familiar prey size when hunting for two sizes of Daphnia magna in high density. This result is not compatible with the apparent size hypothesis. Ten groups were investigated, each consisting of two stickle-backs tested under three consecutive experimental conditions, to establish whether the function of the preference for a familiar prey size could be avoidance of competition.First, the relative competitive ability of each fish was determined by the proportion it consumed of three series of 60 medium-sized daphnia, offered pairwise. Second, during the consumption of five series of 30 pairs, each consisting of a large and a small daphnia, it was determined how many items each fish caught of each prey size. As handling times were equal for both prey types, the larger prey size was more profitable. There was a significant correlation between relative competitive ability and mean proportion of large daphnia in the diet. In the last series the less successful competitiors caught a higher proportion of small prey than in the first series. Finally, each fish was given the choice between large and small daphnia in the absence of its competitor. The sticklebacks chose a diet similar to the one they had been allowed to select previously with competition. The previously more successful competitors concentrated on large daphnia, whereas the poorer competitors fed as generalists but not unselectively.The fish probably learned the distance from which they had recently attacked familiar prey successfully. This sure attack distance depends on the fish's competitive ability.     

Feeding mechanism of Pelagia noctiluca (Scyphozoa: Semaeostomeae); laboratory and open sea observations

The behaviour of the oral arms of Pelagia noctiluca (Forsskål) has been investigated in open sea predation and in predation induced both in the laboratory and the natural environment. Specimens were first studied in the field and then collected from coastal aggregations in the Gulf of Trieste, North Adriatic Sea, in December 1985 (Lat. 13°40 E, Long. 45°42 N) and in June 1986 (Lat. 13°39 E, Long. 45°43 N). The results of laboratory experiments and in situ observations, recorded on videotapes and photographs, show that the marginal tentacles are utilized to: (1) paralyze the prey; (2) contract and bend inward towards the nearest oral arm. Occasionally the tentacle does not contract and the prey is released; thus prey selection may occur. The oral arms are therefore involved in the: (1) transport of prey from the tentacle to the gastric cavity; (2) catching of motionless prey; (3) anchoring the medusa to the substratum. A similar feeding pattern can explain the survival of several specimens of P. noctiluca near the bottom during the winter of 1985/1986 in the Gulf of Trieste (North Adriatic Sea) in spite of the severe climatic conditions.     

Mating success of male hermit crabs in shell generalist and shell specialist species

Summary The reproductive behavior of two species of diogenid hermit crabs was studied in Hawaii. In the shell generalist, Clibanarius zebra, male reproductive success varied little with size, although the largest males were less successful in obtaining copulations than were medium-large males. Male and female size were positively correlated, in successful pairs, thus larger males had the potential to fertilize more eggs when they were successful in obtaining a copulation. Female fecundity in C. zebra was not affected by species of gastropod shell inhabited once female size was taken into account. Male copulatory success was very strongly influenced by the species of gastropod shell inhabited. Males in Trochus or Nerita shells had greatly reduced reproductive success compared to males in Turbo or Nassarius shells. This result was due both to (1) males in Trochus especially dropping and otherwise poorly handling females during precopulatory behavior and (2) females not responding to precopulatory behavior patterns executed by males in Trochus and Nerita. Transferring males from good to bad shapes of shells and vice versa showed that male success was a function of shell type inhabited and not some correlated feature of the crabs. In the shell specialist, Calcinus seurati, which is found primarily in Nerita shells as an adult, males in Nerita shells were quite successful in obtaining copulations.     

Dependence of consumers on macroalgal (Laminaria solidungula) carbon in an arctic kelp community: δ13C evidence

Stable carbon isotope measurements (¹³C) were used to assess the importance of kelp carbon (-13.6 to-16.5) versus phytoplankton carbon (-25.5 to-26.5) to resident fauna of an isolated kelp bed community on Alaska's north arctic coast from 1979 to 1983. The predominant kelp, Laminaria solidungula, showed some seasonal variation in ¹³C which was correlated with changes in the carbon content of the tissue. Animals that showed the greatest assimilation of kelp carbon (>=50%) included macroalgal herbivores (gastropods and chitons,-16.9 to-18.2), a nonselective suspension feeder (an ascidian,-19.0) and a predatory gastropod (-17.6). Animals that showed the least incorporation of kelp carbon into body tissues (<=7%) included selective suspension-feeders (hydroids, soft corals and bryozoans,-22.8 to-25.1). Sponges, and polychaete, gastropod and crustacean omnivores exhibited an intermediate dependence on kelp carbon (15 to 40%). Within some taxonomic groups, species exhibited a broad range in isotopic composition which was related to differences in feeding strategies. In the polychaete group alone, ¹³C values identified four major feeding habits: deposit-feeders (-18.0), omnivores (-20.4), predators (-22.2) and microalgal herbivores (-23.0). Distinct seasonal changes in the ¹³C values of several animals indicated an increased dependence on kelp carbon during the dark winter period when phytoplankton were absent. Up to 50% of the body carbon of mysid crustaceans, which are key prey species for birds, fishes and marine mammals, was composed of carbon derived from kelp detritus during the ice-covered period.     

Response to perceived predation threat in fiddler crabs: trust thy neighbor as thyself?

Prey require information if they are to respond to predation threat in a risk-sensitive manner. One way that individuals can obtain this information is through the predator-mediated, threat-induced behavior of conspecifics. We examined such a possibility in a refuge-seeking species, the sand fiddler crab (Uca pugilator). Crabs were either exposed directly to a simulated predation threat (a moving cylinder) or the threat-induced response of a near neighbor. We found that fiddler crabs responded to the flight of their neighbors even when they, themselves, were not privy to the stimulus that induced their neighbors response. However, the wider range of behaviors exhibited by these crabs—which included no reaction, freezing, running back to the burrow entrance, and burrow retreat—suggest that non-threatened crabs either (1) perceived the gravity of the predation threat differently from their directly threatened neighbors and/or (2) engaged in behaviors that allowed them to acquire further information in the face of uncertainty. Conspecific behaviors also had an effect on the hiding duration of crabs, with individuals hiding longer if they saw both the predation threat and the flight of their neighbor. Our results suggest that cues provided by conspecifics can play an important role in guiding the antipredator response of refuge-seeking prey.     

Dietary constraints on reproductive periodicity in two sympatric deep-sea astropectinid seastars

Examination of the diet of two sympatric species of seastar, Bathybiaster vexillifer and Plutonaster bifrons from 2200 m depth in the Rockall Trough, NE Atlantic Ocean (57°18'N; 10°28'W), suggested that diet may determine the different reproductive patterns found between these two species. In the non-seasonally breeding B. vexillifier, the diet showed a high Shannon-Wiener prey diversity index, the dominant prey being the irregular echinoid Hemiaster expergitus together with a variety of prosobranch gastropods and protobranch bivalves. By contrast, the prey diversity in the seasonally breeding P. bifrons was significantly lower than that of B. vexillifer. In addition, organic carbon content in the sediment residue in the stomachs of P. bifrons displayed a seasonal cycle, while no such seasonality was detected in B. vexillifer. The stomachs of P. bifrons also contained a higher proportion of scavenged material, including the seasonally available remains of mesopelagic blue whiting. These data, together with Bathysnap (time-lapse camera) observations of feeding behaviour in both species, suggest that B. vexillifer is a predator feeding deep in the sediment, whereas P. bifrons feeds close to the sediment surface where it is affected by the seasonal availability of phytodetritus and fish carcasses.     

Primary production as influenced by diel periodicity of phytoplankton photosynthesis

Diel periodicity in parameters of photosynthesis-irradiance (P-I) curves was incorporated into calculations of integral daily phytoplankton production for the Santa Barbara Channel off southern California (USA). Model equations of the relationship between photosynthesis and light were used in combination with observed slope () and asymptote (P _max) values presented in the preceding paper. Primary production was always 19 to 39% less than comparable estimates obtained with the assumption of constant maximum daily and P _max values. Regardless of which P-I formulation was used of 6 tested, observed production (using a temporal series of simulated in situ incubations) ranged from 13% less to 25% more than estimates from constant midday and P _max values. The amplitude and timing of diel oscillations differed somewhat among 3 field stations. Maximum to minimum ratios ranged from approximately 3 to 5 for , and 4 to 6 for P _max. The differences in amplitude and timing of oscillations in P-I curves both contributed to errors in calculating phytoplankton production. Thus, photosynthetic periodicity in the upwelling area of the Santa Barbara Channel influences phytoplankton production. There were oscillations in both and P _max, and the time-dependence of these parameters should be considered to improve the accuracy of predictive models of primary productivity.     

Problems in the assessment of the package effect in five small phytoplankters

The magnitude of the package effect in five small phytoplankters [Thalassiosira sp., Clone 2601 (an unidentified eucaryote), Nannochloris atomus, Synechococcus Syn and Synechococcus WH 7803] was assessed by comparison of the absorption spectra of intact and disrupted cells. The package effect was considerably reduced with reductions in cell size and this was broadly in agreement with theoretical predictions based on Mie theory. However, the quantitative assessment of the package effect is confounded by an inability to assign attenuation (apparent absorption) measurements at =750 nm to either scattering or absorption. The magnitude of the apparent absorption at =750 nm was greatest with the smallest picoplankton species examined, and was reduced, but not eliminated, after cell disruption. Whilst the apparent absorption at =750 nm is commonly thought to be due to residual scattering losses, the available evidence does not exclude the possibility that this may be due in part to absorption by cells or cell constituents and this requires further examination. Although these difficulties are particularly evident with the small picoplankton species, there is no reason to expect that they will not complicate the assessment of the package effect in larger phytoplankton cells.     

Migrational orientation in two species of littoral gastropods (Littorina angulifera and Nerita senegalensis)

Littorina angulifera (Lamark) and Nerita senegalensis Gmelin, both intertidal gastropods, appear to have a preference for specific levels of the shore. In some experiments conducted both in the laboratory and in the field, displaced snails exhibited a general homing behaviour when displaced upshore or downshore. Cues involved in this migrational behaviour have not clearly been identified.     

β-glucanase activity in the seastar Pisaster ochraceus

Specimens of Pisaster ochraceus, an intertidal carnivorous seastar, were collected in northern Puget Sound at Post Point, near Bellingham, Washington, USA, in November 1993, and used in experimentation through September 1994. Ammonium sulfate fractions (60% precipitates) from excised pyloric caeca (digestive glands) possessed -glucanase activity directed toward such -linked substrates as carboxymethylcellulose (CMC), partially-digested cellulose (cellodextrins) and the synthetic substrate p-nitrophenyl--D-glucoside (PNDG). pH-activity and pH-stability maxima were at pH 6 and 5, respectively. No hydrolytic activity was detectable on native cellulose, native chitin or on cationic-modified celluloses such as DEAE-cellulose. Gel filtration (Sephadex G-100) of this 60% precipitate followed by DEAE-Sephadex column chromatography revealed heterogeneity of -glucanase activity, with an indication of up to 11 distinct fractions with variable activity directed toward the substrates indicated above (CMC, cellodextrins and PNDG). These fractions displayed differing ratios of hydrolytic activity on these substrates. None of the isolated fractions displayed activity toward either native cellulose or native chitin. Gut extracts from the edible mussel (Mytilus edulis), a common prey species of P. ochraceus, were shown to possess partially digested cellulose (cellodextrins). These cellodextrins were further degraded by seastar -glucanases in vitro. The digestion of the gut contents of ingested prey provides a possible adaptive explanation for the presence of -glucanase activity in a carnivore.     

Capillary supply and mitochondrial volume density in the axial muscles of the mesopelagic teleost Argyropelecus hemigymnus

Quantitative measurements have been made on the ultra-structure and capillary supply to the axial muscles of the mesopelagic hatchet fish Argyropelecus hemigymnus (Cocco, 1829). Fish were collected at Eastern North Atlantic Ocean Station 10244, 32°48N; 31°15W during November 1980, from a depth of 480 to 550 m. Mitochondria with densely packed cristae occupy 44.3% of slow-fibre volume. Each myofibril is in direct contact with a mitochondrion. Compared with other fishes studied, the capillary supply to A. hemigymnus slow fibres is poorly developed. The average number of capillaries per fibre is 0.9, such that each m of capillary contact supplies 0.011 m² of fibre cross-sectional area. The capillary surface area (m²) supplying 1 m³ of slow-fibre mitochondria is 0.17 in anchovy (Engraulis encrasicolus), 0.14 in rat-fish (Chimaera monstrosa), 0.14 in tench (Tinca tinca), 0.16 in catfish (Clarias mossambica), and only 0.025 for A. hemigymnus. It is suggested that, relative to the former species, some modifications in factors determining tissue oxygenation (e.g. myoglobin concentrations, blood flow, perfusion distribution or haemoglobin) and/or mitochondrial respiration rate are required in order to match oxygen supply and demand to the slow muscle in A. hemigymnus.     

Studies on the nutrition of marine flatfish. The effect of dietary fatty acids on the tissue fatty-acids of the plaice Pleuronectes platessa

The ratios of polyunsaturated fatty acids in the lipids of hatchery-reared plaice [Pleuronectes platessa (L.)] maintained on a diet containing corn oil and freeze-dried cod muscle accurately reflect ratios of polyunsaturated fatty acids in the diet. Such plaice have much greater levels of triglycerides in both their livers and extrahepatic tissues than wild plaice of comparable size; the total amount of 22:6 3 acid in triglycerides of the two groups is, however, very similar. Transfer of experimental plaice to a fat-free diet caused a marked depletion of triglycerides from both livers and extrahepatic tissues with almost all fatty acids being reduced in amount. Addition of 12:0 and 14:0 ethyl esters (4% of each) to the fat-free diet caused some accumulation of triglycerides in extrahepatic tissues. Further addition of 18:2 6 and 18:3 3 acids (both at levels of 0.4%) to the diet caused an accumulation of triglycerides in the liver. These triglycerides had increased levels of saturated and monounsaturated fatty acids up to C 18 but the levels of higher polyunsaturated fatty acids were not inereased. There was relatively little change in the fatty acids of phospholipids throughout these experimental dietary regimes. The levels of phospholipids were constant and very similar to those of wild plaice. The results indicate: (1) plaice fed a diet relatively low in 22:6 3 acid may accumulate relatively large amounts of triglyceride in order to store adequate amounts of 22:6 3 acid; (2) plaice are capable of synthesising saturated and mono-unsaturated fatty acids up to C 18 in the presence of dietary 12:0 and 14:0 acids; (3) 18:2 6 and 18:3 3 acids in the diet do not undergo chain elongation and further desaturation in plaice with the dietary regimes we used.     

Response of the predatorRhizophagus grandis to host plant derived chemicals inDendroctonus micans larval frass in wind tunnel experiments (Coleoptera: Rhizophagidae,Scolytidae)

Summary Adults of the predatory beetleRhizophagus grandis are strongly attracted to both adult and larval frass of its specific prey,Dendroctonus micans, in walking bioassays. Spruce bark and resin are relatively unattractive. Solvent extracts of larval frass that were attractive toR. grandis adults in a flight wind tunnel contained a mixture of monoterpenes of host plant origin. A synthetic mixture of these monoterpenes, (+)—-pinene, (–)—-pinene, -phellandrene,dl limonene and 3-carene, was responsible for 70–80% of the activity of the most attractive extract. We suggest that a blend of monoterpenes in frass acts as a kairomone forR. grandis but that attraction to monoterpenes is only the first of a sequence of behavioural responses that ensures successful host location and identification.     

Predation on penaeid prawns by fishes in Albatross Bay,Gulf of Carpentaria

Fishes were trawled from Albatross Bay, on the west coast of Cape York, north Queensland (12°45S; 141°30E) during 4 yr, from August 1986 to April 1989. Penaeids were the first or second most important prey item by dry weight in 14 of the 34 penaeid-eating fish species, and in 12 of the species by frequency of occurrence. Eighteen species of Penaeidae were identified in fish stomachs. The five commercially important species comprised over 70% by dry weight of all the penaeids eaten by all the fishes;Metapenaeus ensis, Penaeus semisulcatus andP. merguiensis comprised 22, 28 and 11%, respectively. Commercially unimportant penaeids comprised 85% by numbers of all penaeids eaten. Larger fishes ate larger penaeids, mainly commercially important species, while smaller fishes ate smaller penaeids, mainly commercially unimportant species. All penaeid-eating fishes also ate some teleost prey and many were primarily piscivorous. Most penaeid-eating fish species took more benthic prey than bentho-pelagic and pelagic prey combined. The fishes with the strongest predation impact on commercially important penaeids wereCaranx bucculentus and four species of elasmobranchs. The highest impact on commercially unimportant penaeids was made by several species of smaller but abundant fishes. An overall annual estimate of 2950 t yr^–1 of commercially important penaeids is eaten by all fishes, a much higher figure than the average 870 t yr^–1 taken by the fishery. This study highlights the need for accurate measurement of the abundance of penaeid predators as well as analyses of their diets when assessing the impact of predators on prawn stocks.     

Aggression in female Red-Winged Blackbirds: A strategy to ensure male parental investment

Summary Female Red-winged Blackbirds (Agelaius phoeniceus) are often aggressive towards conspecific females during the breeding season. We hypothesize that the function of female-female aggression in this species is to guard the nonshareable portion of the male's parental investment.The investment-guarding hypothesis predicts that a female should be more aggressive toward another female evincing interest in mating with the territory-owning male than toward a female simply perching within the male's territory. Results of mount presentations to females with active nests confirmed this prediction. Nesting females attacked a stuffed conspecific female mounted in a precopulatory, soliciting posture significantly more often than a mount in a normal, perched posture.The male's nonshereable parental care consists of provisioning his young, and most of this care is invested in the brood of his primary (first-to-nest) female. It is therefore predicted that primary females should be more aggressive than secondary (later-nesting) females. Female mount presentations also confirmed this prediction. Primary females attacked the soliciting mount significantly more often than secondary females.     

Prey capture and structure of the visual space of an insect that hunts by sight on the litter layer (Notiophilus biguttatus F., Carabidae,Coleoptera)

Summary The carabid beetle Notiophilus biguttatus hunts springtails and mites by visual cues. The preycapture behaviour of the beetle and the escape behaviour of the springtails were analysed by means of highspeed films. N. biguttatus has between 900 and 1250 ommatidia in each compound eye. The visual space covers ca. 200° in the horizontal plane, with a binocular overlap of no more than 74°. The fovea, the part of the eye where the pseudopupil is largest, points straight ahead of a beetle in its normal posture.The structure of the visual space was determined from measurements of the optical axes in the horizontal plane (plane of fixation) over the middle of the eye. Because of the slanted position of the ommatidia under the cornea, the optical axes point more towards the front or the back of the animal than do the anatomical axes.The optical axes were used to construct the binocular visual space in the horizontal plane. The point E , to which an estimation of distance is possible, lies on the midline 42.6 mm away from the front edges of the eyes. Resolution rapidly decreases with increasing distance, particularly depth resolution.At a distance corresponding to that from which the beetle attacks its prey, depth and width resolution correspond roughly to the dimensions of the smallest prey animals. The smallest measured directional corrections made by the beetle prior to attack (2°–3°) correspond approximately to the divergence angles in the fovea ( _h=2.2°), and the smallest measured distance correction prior to attack (0.2 mm) corresponds approximately to the depth resolution at attack distance.Supported by the Deutsche Forschungsgemeinschaft (SFB 4)     

Feeding factors for the sea anemone Anthopleura midorii

The carnivorous feeding behavior of the sea anemone Anthopleura midorii was found to be a sequence of successive feeding motions, which consisted of several separate actions: (1) tentaculation on any solid matter; (2) retention of prey by tentacles; (3) mouth opening; (4) ingestion of food; (5) digestion of food; (6) extrusion of indigestible waste material. Hot water extract of a natural prey induced the entire feeding sequence when extract was given in the form of an artificial food by mixing with -potato starch and water. The fractionated extract induced only a limited feeding action. Several amino acids induced feeding. Alanine, glycine or histidine stimulated the retention of artificial food by tentacles, and proline evoked mouth opening. Cysteine or reduced glutathione (GSH) induced food ingestion. Unnatural food stuff such as -potato starch was ingestible, if an appropriate amount of a chemical substance was added.