白腐真菌反应器处理废水的研究进展

介绍了白腐真菌的生物学特性及其降解污染物的优势，综述了生物转盘反应器、搅拌釜生物反应器、气升式生物反应器、流化床生物反应器、填充床生物反应器、转管生物反应器、空心纤维反应器等白腐真菌反应器的结构特点和在废水处理方面的研究进展，指出了目前白腐真菌反应器应用中存在的问题。     

Infrared and nuclear magnetic resonance evidence of degradation in thermoplastics based on forest products

The degradation of lignin-(1-phenylethylene) graft copolymers (lignin-styrene graft copolymers) by white rot basidiomycete fungi was followed by monitoring aromatic absorption bands by Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance (NMR) spectroscopy. The FTIR of the graft copolymers shows a series of characteristic absorbance peaks from multi-substituted aromatic rings and a strong poly(1-phenylethylene) (polystyrene) absorbance peak from monosubstituted aromatic rings. Subtraction of copolymer spectra taken before incubation from spectra taken after 50 days of incubation with the four tested fungi shows the loss of functional groups from the copolymer. NMR spectra also show reduction of aromatic ring resonances from the copolymer and incorporation of peaks from fungi as a result of incubation with fungi. The biodegradation tests were run on lignin-(1-phenylethylene) graft copolymers which contained 10.3, 32.2, and 50.4% of lignin. The polymer samples were incubated with the white rot fungiPleurotus ostreatus, Phanerochaete chrysosporium, andTrametes versicolor, and the brown rot fungusGleophyllum trabeum. White rot fungi degraded the plastic samples at a rate that increased with increasing lignin content in the copolymer sample. Both poly(1-phenylethylene) and lignin components of the copolymer were readily degraded. Observation by scanning electron microscopy of incubated copolymers showed a deterioration of the plastic surface. The brown rot fungus did not affect any of these plastics, nor did any of the fungi degrade pure poly(1-phenylethylene).Paper presented at the Bio/Environmentally Degradable Polymer Society—Second National Meeting, August 19–21, 1993, Chicago, Illinois.     

白腐真菌用于有机废水处理的研究

介绍了对污染物有独特降解作用的白腐真菌,阐述了其降解污染物的机理和优势。利用该菌对几种难以用一般生物方法处理的工业废水进行了处理试验,试验结果表明,这种真菌对多种污染物有良好的降解性能,显示出广阔的应用前景。     

Evaluation of white‐rot fungi for the remediation of creosote‐contaminated soil

Application of fungal‐based bioaugmentation was evaluated for the remediation of creosote‐contaminated soil at a wood‐preserving site in West Virginia. Soil at the site contained creosote‐range polycyclic aromatic hydrocarbons (PAHs) at concentrations in some areas that exceed industrial risk‐based levels. Two white‐rot fungi (Pleurotus ostreatus and Irpex lacteus) were evaluated for remediation effectiveness in a two‐month bench‐scale treatability test. Both fungi produced similar results, with up to 67.3 percent degradation of total PAHs in 56 days. Pilot‐scale testing was performed at the site using Pleurotus ostreatus grown on two local substrate mixtures. During the 276‐day field trial, total PAHs were degraded by up to 93.2 percent, with all individual PAHs except one achieving industrial risk‐based concentrations. It was recommended that fungal‐based remediation be applied to all contaminated soil at the site. © 2002 Wiley Periodicals, Inc.     

Use of fungal technology in soil remediation: A Case Study

Two white rot fungi Irpex lacteus and Pleurotus ostreatus and a PAH-degrading bacterial strain of Pseudomonas putida were used as inoculum for bioremediation of petroleum hydrocarbon-contaminated soil from a manufactured-gas-plant-area. Also two cocultures comprising a fungus with Pseudomonas putida were applied. After 10-week treatment out of 12 different PAHs, concentration of phenanthrene, anthracene, fluoranthene and pyrene decreased up to 66%. The ecotoxicity of the soil after bioremediation did not reveal any effect on the survival of Daphnia magna, a crustacian. However, the toxic effect on seed germination of plant Brassica alba and oxidoreductase activity of bacterium Bacillus cereus decreased after 5 and 10 weeks of treatment.     

稻草末固定白腐菌处理染料废水的研究

对采用微电解－白腐菌生物降解－石炭絮凝系统处理活性染料废水进行了研究。试验结果表明，废水的COD去除率和色度除率均达到95％以上，出水水质符合废水排放标准。     

Biodegradation of low-density polyethylene (LDPE) by isolated fungi in solid waste medium

In this study, biodegradation of low-density polyethylene (LDPE) by isolated landfill-source fungi was evaluated in a controlled solid waste medium. The fungi, including Aspergillus fumigatus, Aspergillus terreus and Fusarium solani, were isolated from samples taken from an aerobic aged municipal landfill in Tehran. These fungi could degrade LDPE via the formation of a biofilm in a submerged medium. In the sterilized solid waste medium, LPDE films were buried for 100 days in a 1-L flask containing 400 g sterile solid waste raw materials at 28 °C. Each fungus was added to a separate flask. The moisture content and pH of the media were maintained at the optimal levels for each fungus. Photo-oxidation (25 days under UV-irradiation) was used as a pretreatment of the LDPE samples. The progress of the process was monitored by measurement of total organic carbon (TOC), pH, temperature and moisture. The results obtained from monitoring the process using isolated fungi under sterile conditions indicate that these fungi are able to grow in solid waste medium. The results of FT-IR and SEM analyses show that A. terreus and A. fumigatus, despite the availability of other organic carbon of materials, could utilize LDPE as carbon source. While there has been much research in the field of LDPE biodegradation under solid conditions, this is the first report of degradation of LDPE by A. fumigatus.     

Degradation of Poly(acrylic acid)s with Phenolic Side-Chains by Manganese Peroxidase from White Rot Fungi

Poly(acrylic acid)s (PAAs) with various functional groups, such as phenolic hydroxyl, amino, and aldehyde groups, in the side-chains were treated with manganese peroxidase (MnP) prepared from the culture of lignin-degrading white rot fungi. While no change in the M_w of PAA without a functional group was observed after a 24-h MnP treatment, the M_w␣of␣PAA␣with p-aminophenol as side-chains decreased from 90,000 to 59,000, and that with␣o-aminophenol from 70,000 to 26,000. MnP treatment also decreased the M_w of PAA with a p-aminoaniline or aldehyde group. Furthermore, the MnP treatment generated a significant depolymerization of the cross-linked PAA with p-aminophenol from an insoluble polymer to water soluble products. These results suggest that functional groups generating radicals can act as elemental devices and induce degradation of the PAA main chain.     

Laboratory validation study of new vapor‐phase‐based approach for groundwater monitoring

Recent improvements in field‐portable analytical equipment allow accurate on‐site measurement of VOCs present in air at concentrations of less than 0.1 parts per million volume (ppmv). The objective of this project is to determine if the use of these instruments for vapor‐phase measurements of headspace in a monitoring well can serve as a reliable and accurate method for monitoring volatile organic compound (VOC) concentrations in groundwater under equilibrium conditions. As part of a comprehensive research project investigating the utility of this proposed monitoring method, the authors have completed a laboratory validation study to identify instruments and sample‐collection methods that will provide accurate measurement of VOC concentrations in groundwater. This laboratory validation study identified two field‐portable instruments (a gas chromatograph and a photoionization detector) with sufficient sensitivity to measure VOCs in groundwater at concentrations below typical monitoring standards (i.e., 1 to 5 μg/L). The accuracy and precision of these field instruments was sufficient to satisfy typical data‐quality objectives for laboratory‐based analysis. In addition, two sample‐collection methods were identified that yield vapor‐phase samples in equilibrium with water: direct headspace sampling and passive diffusion samplers. These sample‐collection methods allow the field instruments (which measure VOC concentrations in vapor‐phase samples) to be used to measure VOC concentrations in water. After further validation of these sample‐collection methods in the field, this monitoring method will provide a simple way to obtain accurate real‐time measurements of VOC concentrations in groundwater using inexpensive field‐portable analytical instruments. © 2009 Wiley Periodicals, Inc.     

A New Perspective on Sustainable Soil Remediation—Case Study Suggests Novel Fungal Genera Could Facilitate in situ Biodegradation of Hazardous Contaminants

Deciding upon a cost effective and sustainable method to address soil pollution is a challenge for many remedial project managers. High pressure to quickly achieve cleanup goals pushes for energy‐intensive remedies that rapidly address the contaminants of concern with established technologies, often leaving little room for research and development especially for slower treatment technologies, such as bioremediation, for the more heavily polluted sites. In this case study, new genomic approaches have been leveraged to assess fungal biostimulation potential in soils polluted with particularly persistent hydrophobic contaminants. This new approach provides insights into the genetic functions available at a given site in a way never before possible. In particular, this article presents a case study where next‐generation sequencing has been used to categorize fungi in soils from the Atlantic Wood Industries Superfund site in Portsmouth, Virginia. Data suggest that original attempts to harness fungi for bioremediation may have focused on fungal genera poorly suited to survive under heavily polluted site conditions, and that more targeted approaches relying on native indigenous fungi which are better equipped to survive under site‐specific conditions may be more appropriate. ©2016 Wiley Periodicals, Inc.     

Norwegian Testing of Emulsion Properties at Sea--The Importance of Oil Type and Release Conditions

This paper is a review of the major findings from laboratory studies and field trials conducted in Norway in recent years on the emulsification of oils spilled at sea. Controlled bench-scale and meso-scale basin experiments using a wide spectrum of oils have revealed that both the physico-chemical properties of the oils and the release conditions are fundamental determinants of the rate of emulsion formation, for the rheological properties of the emulsion formed and for the rate of natural dispersion at sea.During the last decade, several series of full-scale field trials with experimental releases of various crude oils have been undertaken in the North Sea and the Norwegian Sea. These have involved both sea surface releases, underwater pipeline leak simulations (release of oil under low pressure and no gas) and underwater blowout simulations (pressurized oil with gas) from 100 and 850 m depth. The field trials have been performed in co-operation with NOFO (Norwegian Clean Seas Association for Operating Companies), individual oil companies, the Norwegian Pollution Control Authority (SFT) and Minerals Management Services (MMS). SINTEF has been responsible for the scientific design and monitoring during these field experiments. The main objectives of the trials have been to study the behaviour of different crude oils spilled under various conditions and to identify the operational and logistical factors associated with different countermeasure techniques.The paper gives examples of data obtained on the emulsification of spilled oil during these field experiments. The empirical data generated from the experimental field trials have been invaluable for the validation and development of numerical models at SINTEF for predicting the spreading, weathering and behaviour of oil released under various conditions. These models are extensively used in contingency planning and contingency analysis of spill scenarios and as operational tools during spill situations and combat operations.     

改进型气升式反应器中白腐菌降解碱木素

设计了一种白腐菌糙皮侧耳高效降解废水中碱木素的改进型气升式反应器,研究了内套管的开孔高度与脱色率的关系,并采用分批进料方式考察了改进后反应器中白腐菌对碱木素的降解效果。实验结果表明：反应器内套管的最佳开孔高度为45 cm;改进后反应器的漆酶酶活、COD去除率、脱色率分别最高达到了89.33 U/L、87.3%和81.39%,较改进前分别相对提高了17.5%、43.3%和39.6%。     

Fouling and Degradation of Polycarbonate in Seawater: Field and Lab Studies

Biofouling and ensuing microbial mediated degradation of Bisphenol A polycarbonate was studied by immersing the samples in sea water of Bay of Bengal (Chennai, India) for 3 months and also under controlled laboratory conditions with marine mixed microbial consortia for 12 months. A 9% weight loss in the sample was observed after 1 year of incubation under in vitro laboratory conditions. A 5% reduction in number average molecular weight and an additional oligomer with a molecular weight of 930 was observed in the same sample. Contact angle decreased by 11% indicating an increase in the surface hydrophilicity. The specific heat decreased by 44% and glass transition temperature decreased by 3 °C with respect to the control indicating chain scission. Formation of new hydroxyl groups and cleavage of carbonate bonds in polycarbonate suggested biodegradation. About 9 μg mL⁻¹ of Bisphenol A, a monomer of polycarbonate, as well as its oxidized products were detected in the supernatant. The nature of degradation in field and in vitro was different. It was predominantly oxidation in the former and hydrolysis in the later environment. A strain exhibiting hydrolase activity was isolated at the end of the 12 months from the in vitro mixed consortia and was identified, based on biochemical and 16S rDNA tests, as Pseudomonas sp. BP2 (GenBank accession no. EU920674).     

Modelling of Flow and Pollution Dispersion in a Two Dimensional Urban Street Canyon

The wind-driven flow patterns and the dispersion of vehicle exhaust pollutants released at street level has been simulated with the three-dimensional (3-D) dispersion model ADREA-HF (Andronopoulos et al., 1993), for idealised two-dimensional urban fetches occupied by buildings with slanted roofs. The simulation used oncoming atmospheric boundary layer characteristics corresponding to realistic above-town wind characteristics, as measured in reference wind tunnel experiments (Rafailidis, 1997). At that stage, analysis was limited to neutral stability conditions only. Firstly, the quality assurance of the numerical model was investigated in terms of the sensitivity to different grid allocations. The modelling results were corroborated by comparison with wind tunnel measurements in a similar two-dimensional domain (Pavageau et al., 1997). The numerical modelling replicated well the high degree of non-uniformity in the dispersion field in the test street, and the results agreed satisfactorily with the experimental measurements. The reasons for the differences observed have been investigated. With the model thus validated, three different exhaust release scenarios have been tested, keeping the same overall emission rate but different spatial patterns of street-release. The effect of the different street-release scenarios was found to be only marginal, with the dispersion patterns on the sidewalls affected only locally, close to the street level.     

Hype or Hope: The Potential of Phytoremediation as an Emerging Green Technology

Phytoremediation is defined as the use of green plants and their associated microorganisms, soil amendments, and agronomic techniques to remove, contain, or render harmless environmental pollutants. At the present time, phytoremediation is an emerging technology and there is still a significant need to pursue both fundamental and applied research to fully exploit the metabolic and growth habits of higher plants. It is precisely the purpose of the European COST Action 837 to stimulate the development and evaluate the potential of plant biotechnology for the removal of organic pollutants and toxic metals from wastewater and contaminated sites. However, green plants grow under nonsterile conditions and thus strongly interact with many microorganisms, like bacteria and mycorrhizal fungi. In this context, an Inter‐COST Workshop on bioremediation was recently organized to address the significance of soil microorganisms for plants, and the importance of their interactions, with regard to their potential for phytoremediation. Based on the outcomes of this workshop, the potential use of phytoremediation is presented in this article. © 2001 John Wiley & Sons, Inc.     

Coupling Flow Analysis With Geochemical and Microbiological Analyses to Assess the Bioremediation Feasibility of a Contaminated Aquifer

An integrated approach combining classic and molecular microbiological methods, “in vitro” bioremediation assays and groundwater numerical modeling, has been established to identify optimized solutions for remediating aquifers contaminated with organic pollutants. Bacteria have been isolated from an aquifer contaminated with toluene and methyl tert‐butyl ether (MTBE), selected for their growth with contaminants as a sole carbon source and identified through 16S rDNA partial sequencing. Successive biodegradation laboratory tests have been performed to determine which chemical conditions were more appropriate for the isolated bacteria to more efficiently oxidize toluene and MTBE. A groundwater model was created using FEFLOW code first to determine the movement of the plume front and second to simulate the impact of the biodegradation processes along the groundwater flow directions based on the bioremediation rates obtained in the laboratory. The results show that this innovative and interdisciplinary model can be used to assist in developing monitoring and remediation plans for cleaning up complex contaminated groundwater sites. This approach successfully combines the identification of the optimum biogeochemical conditions for bacterial biodegradation to occur with the predictability of the development of the process over time, ensuring decisive support in the management of contaminated sites. ©2016 Wiley Periodicals, Inc.     

Uptake and Metabolism of TNT and GTN by Plants Expressing Bacterial Pentaerythritol Tetranitrate Reductase

The manufacture and improper disposal of explosives has resulted in a significant amount of land requiring remediation. The compound 2,4,6-trinitrotoluene (TNT) is the most persistent and toxic of the explosive pollutants with current treatment methods being energy intensive and costly. Bacterial enzymes such as pentaerythritol tetranitrate reductase (PETNR) from Enterobacter cloacae PB2 have been found to have activity against TNT; however, microbes often lack the biomassnecessary for remediation applications. The PETNRgene (onr1) was transformed into tobacco plants in an attempt to combine the metabolic diversity of microbes with the sequestering properties of plants. The resulting transgenic plants were shown to have enhanced tolerance to TNT during germination and as seedlings. Phytoremediation applications with these plants may provide an alternative treatment of TNT contamination.     

Scaling methane oxidation: from laboratory incubation experiments to landfill cover field conditions

Evaluating field-scale methane oxidation in landfill cover soils using numerical models is gaining interest in the solid waste industry as research has made it clear that methane oxidation in the field is a complex function of climatic conditions, soil type, cover design, and incoming flux of landfill gas from the waste mass. Numerical models can account for these parameters as they change with time and space under field conditions. In this study, we developed temperature, and water content correction factors for methane oxidation parameters. We also introduced a possible correction to account for the different soil structure under field conditions. These parameters were defined in laboratory incubation experiments performed on homogenized soil specimens and were used to predict the actual methane oxidation rates to be expected under field conditions. Water content and temperature corrections factors were obtained for the methane oxidation rate parameter to be used when modeling methane oxidation in the field. To predict in situ measured rates of methane with the model it was necessary to set the half saturation constant of methane and oxygen, K_m, to 5%, approximately five times larger than laboratory measured values. We hypothesize that this discrepancy reflects differences in soil structure between homogenized soil conditions in the lab and actual aggregated soil structure in the field. When all of these correction factors were re-introduced into the oxidation module of our model, it was able to reproduce surface emissions (as measured by static flux chambers) and percent oxidation (as measured by stable isotope techniques) within the range measured in the field.     

Water Quality Assessment by Ecotoxicological and Chemical Methods in Magnesia,Greece

The fresh- and seawater quality in Magnesia, Greece, was evaluated in this work, using a combination of bioassays and chemical analytical techniques. The crustacean and the invertebrate saline water shrimp Artemia franciscana were used as test species for monitoring the quality of fresh- and seawater samples respectively. Furthermore, chemical analysis was performed in the samples, for the assessment of pollutants loading. In general, both fresh- and seawater toxicity was low, indicating the good environmental conditions in the area. However, two seawater samples and one freshwater sample, presented high toxicity values, which is in agreement with the chemical measurements.     

Factors affecting polycyclic aromatic hydrocarbon biodegradation by Aspergillus flavus

This study investigated the ability of fungi isolated from highly contaminated soil to biodegrade polycyclic aromatic hydrocarbon (PAH) compounds, as well as the effect of several parameters on the biodegradation ability of these fungi. The isolated fungi were identified using ITS rDNA sequencing and tested using 2,6‐dichlorophinolendophenol to determine their preliminary ability to degrade crude oil. The top‐performing fungi, Aspergillus flavus and Aspergillus fumigatus, were selected to test their ability to biodegrade PAH compounds as single isolates. After 15 days of incubation, A. flavus degraded 82.7% of the total PAH compounds, with the complete degradation of six compounds, whereas A. fumigatus degraded 68.9% of the total PAHs, with four aromatic compounds completely degraded. We also tested whether different temperatures, pH, and nitrogen sources influenced the growth of A. flavus and the degradation rate. The degradation process was optimal at a temperature of 30°C, pH of 5.5, and with nitrogen in the form of yeast extract. Finally, the ability of the fungal candidate, A. flavus, to degrade PAH compounds under these optimum conditions was studied. The results showed that 95.87% of the total PAHs, including 11 aromatic compounds, were completely degraded after 15 days of incubation. This suggests that A. flavus is a potential microorganism for the degradation of PAH compounds in aqueous cultures.