Sludge-grown algae for culturing aquatic organisms: Part I. Algal growth in sludge extracts

This project is aimed at studying the feasibility of using sewage sludge to prepare culture media for microalgae (Chlorella-HKBU) and the use of the sludge-grown algae as a feed for some aquatic organisms. Part I of the project included results on preparing sludge extracts and their use on algal culture. By comparing two culturing techniques, aeration and shaking, it was noted that both lag and log phases were shortened in the aeration system. A subsequent experiment noted that algal growth subject to aeration rates of 1.0 and 1.5 liters/min had similar lag and log phases. In addition, both aeration rates had a significantly higher (P < 0.05) final cell density than that of 0.5 liters/min. A detailed study on the variation of growth conditions on the algal growth was done. The results indicated that pH values of all the cultures declined below 5 at day 12. The removal rates of ammonia N ranged from 62% to 70%. The sludge-grown algae contained a rather substantial amount of heavy metals (µg/g): Zn 289–581, Cu 443–682, Ni 310–963, Mn 96–126, Cr 25–118, and Fe 438–653. This implied that the rather high levels of heavy metals may impose adverse effects on higher trophic organisms.     

Anaerobic/aerobic treatment of meat processing wastewater

The meat processing industry is believed to produce highly polluted wastewater. Analysis of such wastewater indicated that the waste was highly contaminated with organic compounds as indicated by COD (1544mgO2l–1), BOD (646mgO2l–1), and TSS (1155 mgl–1). Moreover, oil and grease concentrations reached 144mgl–1 treatment of raw wastewater using Upflow Anaerobic Sludge Blanket (UASB) followed by Rotating Biological Contactors (RBC) was studied. Efficiency of the UASB for the removal of CODtotal, BODtotal, TSS, and oil and grease was 56%, 56%, 85%, and 58%, respectively. The quality of the UASB effluent barely complies with the regulatory standards for discharging wastewater into the sewerage network. UASB effluent was subjected for further treatment using a RBC unit to improve the quality of the treated effluent for reuse in irrigation purposes. Residual COD, BOD, TSS, and oil and grease, following RBC, was 132mgO2l–1, 40mgO2l–1, 44mgl–1, and 10mgl–1 respectively. The overall efficiency of the treatment units provided good quality effluent. The overall percentage removal of COD, BOD, TSS, and oil and grease was 91.5%, 94%, 96%, and 91%, respectively. Based on the quality of the treated effluent and guidelines recommended for wastewater reuse, it may be concluded that a slight to moderate restricted irrigation is applicable to reuse the treated effluent in the green belt around the factory. Disinfection should be applied to ensure the safety of such a process.     

Use of constructed wetland for the removal of heavy metals from industrial wastewater

This study was conducted to investigate the effectiveness of a continuous free surface flow wetland for removal of heavy metals from industrial wastewater, in Gadoon Amazai Industrial Estate (GAIE), Swabi, Pakistan. Industrial wastewater samples were collected from the in-let, out-let and all cells of the constructed wetland (CW) and analyzed for heavy metals such as lead (Pb), cadmium (Cd), iron (Fe), nickel (Ni), chromium (Cr) and copper (Cu) using standard methods. Similarly, samples of aquatic macrophytes and sediments were also analyzed for selected heavy metals. Results indicate that the removal efficiencies of the CW for Pb, Cd, Fe, Ni, Cr, and Cu were 50%, 91.9%, 74.1%, 40.9%, 89%, and 48.3%, respectively. Furthermore, the performance of the CW was efficient enough to remove the heavy metals, particularly Cd, Fe, and Cu, from the industrial wastewater fed to it. However, it is suggested that the metal removal efficiency of the CW can be further enhanced by using proper management of vegetation and area expansion of the present CW.     

Utilization of pulp and paper industrial wastes to remove heavy metals from metal finishing wastewater

Two pulp and paper industrial wastes, lime mud (LM) and recovery boiler ash (RB), have low moisture contents, low heavy metal contaminations and contain various carbonate compounds which contribute to a high pH. Metal finishing wastewater (MF-WW) has a low pH, high levels of TDS and high contaminations from Cr, Cu, Pb and Zn. The heavy metals from MF-WW were removed by sorption and precipitation mechanisms. LM gave better results in removing heavy metals from MF-WW than RB. At a reaction time of 45 min, the maximum removal efficiencies for Cr (93%) and Cu (99%) were obtained at 110 g L⁻¹ of LM, but at 80 g L⁻¹ for Pb (96%) and Zn (99%). Treatment with LM gives a higher sludge volume than with RB. However, the leachability of heavy metals from LM is lower. Leachability of heavy metals in the sediment for all selected treatment conditions is within government standards.     

Assessment of the Microbiological Quality of Clarias gariepinus Exposed to an Industrial Effluent in Nigeria

The microbiological impact of a detergent and soap industries effluent on Clarias gariepinus was assessed under laboratory conditions. The heterotrophic bacterial count obtained from fish surfaces ranged from 1.2 × 10²−2.0 × 10²cfu/ml amongst the control, while values of 4.8× 10⁶−8.6 × 10⁶ cfu/ml were obtained for the experimental fish exposed to the industrial effluent (0.025 ppm). The fungal count for the controls ranged from 1.2× 10²−1.2 × 10³ cfu/ml; while a range of 1.0 × 10⁶−2.0 × 10⁶ was obtained for the fish exposed to the industrial effluent. While twelve bacterial species were isolated from the fish exposed to the industrial effluent, only two were isolated from the parts of the control fish used in the study. The bacterial species are those in the genera Staphylococcus, Proteus, Streptococcus, Micrococcus, Bacillus, Pseudomonas, Serratia, Enterobacter, and Escherichia. The fungal isolates include Saccharomyces, Aspergillus, Rhodosporium, Candida, Alternaria, and Fusarium. The resistance of the bacterial isolates to the commonly used antibiotics showed that 100% were resistant to Augmentin, Amoxycillin and Cloxacillin, 85.71% to Tetracycline, 80.95% to Cotrimoxazole, 71.43% to Erythromycin, 33.33% to Chloramphenicol, and 28.57% to Gentamicin. Among the eight antibiotics tested, five patterns of multiple drug resistance were obtained, with the number of the antibiotics ranging from 4–8. The public health implications of these observations are discussed.     

Biosorption of Cu and Zn from aqueous solutions by dried marine green macroalga Chaetomorpha linum

The biosorption of the heavy metals Cu²⁺ and Zn²⁺ by dried marine green macroalga (Chaetomorpha linum) was investigated. The biosorption capacities of the dried alga for copper and zinc were studied at different solution pH values (2–6), different algal particle sizes (100–800 μm) and different initial metal solution concentrations (0.5–10 mM). An optimum pH value of 5 was found suitable for both metal ions biosorption for both metal ions. At the optimum particle size (100–315 μm), biosorbent dosage (20 g/l) and initial solution pH (pH 5), the dried alga produced maximum copper and zinc uptakes values (q_max) of 1.46 and 1.97 mmol/g respectively (according to the Langmuir model). The kinetic data obtained at different initial metal concentrations indicated that the biosorption rate was fast and most of the process was completed within 120 min. This study illustrated an alternative technique for the management of unwanted biological materials using processed algal material. C. linum is one of the fast-growing marine algae in the lake of Tunis and could be utilized as a biosorbent for the treatment of Cu²⁺ and Zn²⁺ contaminated wastewater streams.     

Destruction of Wetlands and Waterbird Populations by Dams and Irrigation on the Murrumbidgee River in Arid Australia

The Lowbidgee floodplain is the Murrumbidgee Rivers major wetland in southeastern Australia. From more than 300,000 ha in the early 1900s, at least 76.5% was destroyed (58%) or degraded (18%) by dams (26 major storages), subsequent diversions and floodplain development. Diversions of about 2,144,000 ML year^–1 from the Murrumbidgee River come from a natural median flow of about 3,380,000 ML year^–1 providing water for Australias capital, hydroelectricity, and 273,000 ha of irrigation. Diversions have reduced the amount of water reaching the Lowbidgee floodplain by at least 60%, from 1888 to 1998. About 97,000 ha of Lowbidgee wetland was destroyed by development of the floodplain for an irrigation area (1975–1998), including building of 394 km of channels and 2,145 km of levee banks. Over 19 years (1983–2001), waterbird numbers estimated during annual aerial surveys collapsed by 90%, from an average of 139,939 (1983–1986) to 14,170 (1998–2001). Similar declines occurred across all functional groups: piscivores (82%), herbivores (87%), ducks and small grebe species (90%), large wading birds (91%), and small wading birds (95%), indicating a similar decline in the aquatic biota that formed their food base. Numbers of species also declined significantly by 21%. The Lowbidgee floodplain is an example of the ecological consequences of water resource development. Yanga Nature Reserve, within the Lowbidgee floodplain, conserved for its floodplain vegetation communities, will lose these communities because of insufficient water. Until conservation policies adequately protect river flows to important wetland areas, examples such as the Lowbidgee will continue to occur around the world.     

Performance assessment of a wastewater treatment plant producing effluent for irrigation in Egypt

A conventional activated sludge treatment facility was the subject of this study. The assessment was directed at determining the characteristics of the raw wastewater, the quality of the treated effluent and the efficiency of the various treatment units. Furthermore, the water quality along the effluent irrigation canal was monitored. The assessment of the quality of the treated effluent for irrigation is based on the guidelines established by the World Health Organization and the Egyptian decree 9/89 for the use of wastewater in agriculture. The results of the study indicated that the concentration of the raw wastewater was considered moderate. The mean values of the COD (Chemical Oxygen Demand), BOD (Biological Oxygen Demand) and total suspended solids (TSS) were around 250, 102 and 142 mg l–1, respectively. This was attributed to the high quantities of wastewater from industrial sources. The overall efficiency of the treatment facility was good. The mean residual COD, BOD and TSS were 25, 8 and 21 mg l–1 and the corresponding percentage removal values were 90, 92 and 85%, respectively. The maximum percentage removal of oil and grease was 84% with a mean residual concentration of 24 mg l–1. The total viable count (22°C and 37°C), faecal coliform and aecal streptococci were reduced by 99.9% compared to only 99.5% for Salmonella. Bacteriological examination of the dried sludge indicated a reduction of nine logs of faecal coliform and faecal streptococci, as compared to thickened sludge. Analysis of the Ni, Cu, Pb and Cr in the dried sludge indicated that their concentrations are within the permissible limits. Zinc exceeded the consent standards by 50%. The results of the analyses of samples collected at the beginning of the irrigation canal indicated insignificant changes from the characteristics of the final effluent. Samples collected at a distance of 2km along the irrigation canal showed mean reductions in the COD and BOD of 28.6 and 47%, respectively, which could be attributed to sedimentation and/or a self-purification effect. An increase in the total nitrogen, total phosphorus and total viable count was also recorded, which could be due to seepage from the agricultural land. From the data available it is evident that the treated wastewater could be used for restricted irrigation. The design and implementation of a monitoring programme is recommended.     

人工湿地-氧化塘工艺组合对氮和磷去除效果研究

本文在小试规模上，研究了下行流湿地、推流床湿地、氧化塘和兼性塘四种处理单元的四种工艺组合对氮、磷的去除效果，研究结果表明：下行流湿地 氧化塘工艺组合具有较好的充氧效果，推流床湿地后置也可以提高出水的溶解氧。四种工艺组合对离子和TP、IP的去除无显著差异。人工湿地中硝化作用的发生有利于NH^ 4f-N的去除，增加氧化塘可以提高系统的硝化能力，但同时也会增加出水中的N0^-3-N浓度。     

Removal and recycling of copper from aqueous solutions using treated Indian barks

Removal of copper from aqueous solutions containing 100–1000 ppm, using different Indian bark species, was performed on laboratory scale. The percentage removal of metal ions depends on the solution pH, bark species and time. The efficiency of copper removal by the used raw barks increases with a rise of solution pH and reaches a maximum of about 65–78% around pH 4–5. However, the decontaminated aqueous solutions were colored due to the dissolution of soluble organic compounds contained in the raw bark. This increases the biological and chemical oxygen demand (BOD and COD) of the solutions as well as the total organic carbon content (TOC). For this reason, raw bark should be treated either by chemical or biological means. Such treatment will allow the extraction of the soluble organic compounds and increase the chelating capacity and efficiency of the treated bark. Depending on the pH value, the chelating efficiency of treated barks is about 1.2–2.2 times that of the raw ones. Moreover, the retention capacity of the Indian treated bark varies from about 42–51 mg/g of dry bark. It is equal to or higher than that of common European species. About 1.8 mols of H₃O⁺ are released, by the treated barks, for every mol of chelated copper ions. Moreover, scanning electron microscopy (SEM) observations show uniform distribution of metal ions throughout the copper saturated bark. Infra red (IR) spectra suggest that the copper ions are chelated to hydroxyl and/or carboxyl functional groups of organic compounds contained in the treated bark. It seems that the interaction of the copper ions with the bark follows a cation exchange mechanism. This hypothesis is supported by elution experiments that allow recovery of about 99% of the contained copper. The retention capacity of the treated bark is almost constant after five cycles of chelation–elution, suggesting that the ‘life time cycle' is sufficiently long for continuous industrial application. The spent copper loaded barks can either be incinerated or pyrolysed. It generates solids containing either ≈80% of CuO or ≈14% of Cu°, respectively. Such materials can be used either in the secondary or primary copper production, thus offering a friendly environmental solution of effluents' treatment. The suggested process can be used as an alternative to the classical technologies for effluent decontamination. It is also efficient for polishing effluents treated by other methods.     

Heavy metal removal and cyanide destruction in the metal plating industry: an integrated approach from egypt

Wastewater produced from a metal plating is a major environmental problem. Industrial auditing revealed that the main source of pollution mainly originated from rinsing water. The characterization of final effluent showed that it is highly contaminated with hazardous heavy metals and cyanide. The concentration of copper, hexavalent chromium, nickel, and cyanide in the rinsing water of metal plating department was 14.8, 40.9, 13.3, and 19 mg/l, respectively. The concentration of cyanide and zinc from the galvanizing department reached 60 and 80 mg/l. The remediation scheme included the application of in-plant control measures via changing the rinsing process followed by the destruction of cyanide and reduction of hexavalent chromium bearing wastes. The pretreated wastes were then mixed with other industrial wastes prior to a combined chemical coagulation-sedimentation using lime and/or lime in combination with ferric chloride. The results indicated that, after applying the waste minimization measures alone at the source, prior to final treatment of industrial waste, removal rates of cyanide, copper, nickel, and chromium concentrations were 23.2%, 14.9%, 32.3%, and 55.3%, respectively in the rinse water from metal plating department. Furthermore, the removal rates of cyanide and zinc in the galvanizing department reached 59.7% and 24.3. The integrated control measures and treatment scheme led to more than 99% removal of copper, nickel, chromium, and zinc, while the complete removal of cyanide was achieved in the final effluent.     

Removal of heavy metals in rinsing wastewater from plating factory by adsorption with economical viable materials

The removal of heavy metals from plating factory wastewater with economical materials was investigated by the column method. Montmorillonite, kaolin, tobermorite, magnetite, silica gel and alumina were used as the economical adsorbents to wastewater containing Cd(II), Cr(VI), Cu(II) and Pb(II). This removal method of heavy metals proved highly effective as removal efficiency tended to increase with increasing pH and decrease with increasing metal concentration. The removal percentages by adsorption onto montmorillonite, tobermorite, magnetite, and silica gel showed high values for all metals. From the results for the heat of adsorption, the adsorption process in the present study might be chemisorption. The proposed method was successfully applied to the removal of Cd(II), Cr(VI) and Cu(II) in rinsing wastewater from plating factory in Nagoya City, Aichi Prefecture, Japan. Since the economical adsorbents used can be obtained commercially because they are easily synthesized, the wastewater treatment system developed is rapid, simple and cheap for the removal of heavy metals.     

Biological nutrient removal from pre-treated landfill leachate in a sequencing batch reactor

Biological treatment of landfill leachate usually results in low nutrient removals because of high chemical oxygen demand (COD), high ammonium-N content and the presence of toxic compounds such as heavy metals. Landfill leachate with high COD content was pre-treated by coagulation-flocculation with lime followed by air stripping of ammonia at pH=12. Nutrient removal from pre-treated leachate was carried out using a lab-scale sequencing batch reactor (SBR). Three different operations consisting of different numbers of steps were tested and their performances were compared. These operations were the three-step anaerobic (An)/anoxic (Ax)/oxic (Ox); the four-step (An/Ox/Ax/Ox), and the five-step (An/Ax/Ox/Ax/Ox) operations with total residence time of seven hours each. Experiments were carried out using three consecutive operations with a total cycle time of 21 h at a constant sludge age of 10 days. The lowest effluent nutrient levels were realized by using the five-step operation which resulted in effluent COD, NH4-N and PO4-P contents of 1,400, 107 and 65 mg l(-1), respectively, at the end of 21 h. Addition of domestic wastewater (1/1, v/v) and powdered activated carbon (PAC, 1 g l(-1)) to the pre-treated leachate improved nutrient removals in the five-step SBR operation, resulting in 75% COD, 44% NH4-N and 44% PO4-P removals after 21 hours of operation.     

Response of Organic and Inorganic Carbon and Nitrogen to Long-Term Grazing of the Shortgrass Steppe

We investigated the influence of long-term (56 years) grazing on organic and inorganic carbon (C) and nitrogen (N) contents of the plant–soil system (to 90 cm depth) in shortgrass steppe of northeastern Colorado. Grazing treatments included continuous season-long (May–October) grazing by yearling heifers at heavy (60–75% utilization) and light (20–35% utilization) stocking rates, and nongrazed exclosures. The heavy stocking rate resulted in a plant community that was dominated (75% of biomass production) by the C₄ grass blue grama (Bouteloua gracilis), whereas excluding livestock grazing increased the production of C₃ grasses and prickly pear cactus (Opuntia polycantha). Soil organic C (SOC) and organic N were not significantly different between the light grazing and nongrazed treatments, whereas the heavy grazing treatment was 7.5 Mg ha^–1 higher in SOC than the nongrazed treatment. Lower ratios of net mineralized N to total organic N in both grazed compared to nongrazed treatments suggest that long-term grazing decreased the readily mineralizable fraction of soil organic matter. Heavy grazing affected soil inorganic C (SIC) more than the SOC. The heavy grazing treatment was 23.8 Mg ha^–1 higher in total soil C (0–90 cm) than the nongrazed treatment, with 68% (16.3 Mg ha^–1) attributable to higher SIC, and 32% (7.5 Mg ha^–1) to higher SOC. These results emphasize the importance in semiarid and arid ecosystems of including inorganic C in assessments of the mass and distribution of plant–soil C and in evaluations of the impacts of grazing management on C sequestration.     

Renovation of food-processing wastewater by a Riparian wetland

Treated wastewater from a food-processing plant, together with intermittent outflow from a hypereutrophic pond, were discharged over a 20-year period to a cattail-dominated wetland and hence to a small stream. Organics and nutriet levels in the effluent were comparable to levels in domestic wastewater. Fifteen variables were monitored upstream and downstream from the plant over 18 months. Means for most variables were slightly higher downstream, but differences between stations were not statistically significant. Wetland processing of nitrogen was markedly affected by a change from drought to flood conditions. After accounting for dilution, the overall effect of the wetland on the effluent was to reduce biological oxygen demand 43.7%, ammonia N 46.3%, nitrate/nitrite N 17.4%, and conductivity 15.6%. However, total suspended solids were increased 41.4%, total organic nitrogen 28.8%, and total phosphorus 24.7%. It was concluded that the wetland effectively renovated the effluent but the removal efficiency would be improved if the effluent were pretreated to reduce phosphorus and dispersed to increase residence time in the wetland.     

Three Egyptian industrial wastewater management programmes

A pre-treatment programme for wastewater from factories, representing three main industrial sectors in Egypt, has been developed. The first case study was a factory producing potato-chips. Wastewater discharged from this factory was characterized by high values of BOD, SS and oil and grease (6000 mgO₂ l^–1, 6577 mg l^–1 and 119 mg l^–1 respectively). Chemical treatment using lime and lime aided by polyelectrolyte achieved good results. Residual values of BOD and SS after treatment were 97 mg l^–1 and 49 mg l^–1, respectively. Oil and grease concentrations were reduced by 91 percent. Treatment via activated sludge at a detention time of 4 hrs produced good quality effluent. The second case study was an automobile company, representing the metal finishing industry. Analyses of wastewater samples from the degreasing, phosphating and painting departments, as well as the end-of-pipe effluent were conducted. The end-of-pipe effluent contained high concentrations of oil and grease (366 mg l^–1), phosphorous (111 mg l^–1) and zinc (81 mg l^–1). Chemical treatment of end-of-pipe wastewater using ferric chloride aided by lime, produced high quality effluent. The third sector was the chemical industry. For this purpose a paint factory was selected. Characteristics of raw wastewater varied widely according to the production rate. Average values of COD and BOD were 1950 mg l^–1 and 683 mg l^–1. Oil and grease ranged from 63 to 1624 mg l^–1. Chemical treatment using ferric chloride in combination with lime at the optimum operating conditions achieved good results. Residual values after treatment of COD, BOD and oil and grease reached 120, 36 and 8.6 mg l^–1, respectively. An engineering design for each case study has been prepared.     

Evaluation of biogas production from seaweed in batch tests and in UASB reactors combined with the removal of heavy metals

Seaweed can be anaerobically digested for the production of energy-rich methane. However, the use of seaweed digestate as a fertilizer may be restricted because of the high heavy metal content especially cadmium. Reducing the concentration of heavy metals in the digestate will enable its use as a fertilizer. In this laboratory-scale study, the potential of seaweed and its leachate in the production of methane were evaluated in batch tests. The effect of removing the heavy metals from seaweed leachate was evaluated in both batch test and treatment in an upflow anaerobic sludge blanket (UASB) reactor. The heavy metals were removed from seaweed leachate using an imminodiacetic acid (IDA) polyacrylamide cryogel carrier. The methane yield obtained in the anaerobic digestion of seaweed was 0.12 N l CH₄/g VS_added. The same methane yield was obtained when the seaweed leachate was used for methane production. The IDA-cryogel carrier was efficient in removing Cd²⁺, Cu²⁺, Ni²⁺ and Zn²⁺ ions from seaweed leachate. The removal of heavy metals in the seaweed leachate led to a decrease in the methane yield. The maximum sustainable organic loading rate (OLR) attained in the UASB reactor was 20.6 g tCOD/l/day corresponding to a hydraulic retention time (HRT) of 12 h and with a total COD removal efficiency of about 81%. Hydrolysis and treatment with IDA cryogel reduced the heavy metals content in the seaweed leachate before methane production. This study also demonstrated the suitability of the treatment of seaweed leachate in a UASB reactor.     

Regeneration of industrial district wastewater using a combination of Fenton process and ion exchange—A case study

Regeneration studies of wastewater effluent from an organized industrial district (OID) for possible reuse in textile industry as process water was investigated. Advanced treatment methods including Fenton process, polyaluminium chloride (PAC) coagulation and ion exchange were applied on OID effluent. In Fenton process removal efficiencies for suspended solids (SS), chemical oxygen demand (COD), SAC₄₃₆ (spectral absorption coefficient), SAC₅₂₅ and SAC₆₂₀ were determined 61%, 36%, 35%, 49% and 67%, respectively. After Fenton process, wastewater samples were coagulated with PAC. Optimum removal efficiencies for SS, COD, Fe ion, SAC₄₃₆, SAC₅₂₅ and SAC₆₂₀ were determined 83%, 18%, 93%, 32%, 36% and 58%, respectively. Ion exchange experiments were conducted on chemically coagulated wastewater samples to improve the quality of wastewater. Optimum dosage of resins was determined. The experiments revealed that 1:1 resin ratio (20 mL H-type resin:20 mL OH-type resin) gave the best removal rates for the parameters considered in this study. Study results indicated that quality of the wastewater was suitable for the process water characteristics of textile industry and unit wastewater treatment cost was determined as 2.54 €/m³.     

人工湿地控制非点源污染的应用

随着点源污染的有效管理和控制，非点源污染已成为水环境污染的主要原因。人工湿地作为一种控制水环境非点源污染的有效工具，已被世界上很多国家所认可。本文首先简述了非点源污染的危害，其次对人工湿地的概念和类型进行了介绍，论述了人工湿地对非点源污染中氮、磷、重金属和农药等主要污染物的去除机理，最后对人工湿地处理系统的附属设施、水力因素、表土层以及植物收割等应用问题进行了探讨。     

Effects of spent engine oil on the growth parameters, chlorophyll and protein levels of Amaranthus hybridus L.

The susceptibility of Amaranthus hybridus L. seedlings to spent engine oil was investigated in soil supplemented with concentrations of oil ranging from 1–5 percent v/w. Parameters considered were relative growth rate (RGR), leaf area ratio (LAR), whole plant height, leaf area, leaf number, chlorophyll and protein levels. A relationship was found to exist between the inhibitory effects and the treatment concentrations. After seventy days growth in the treated soils, the mean height and leaf area of plants in soils treated with 5 percent spent engine oil were 27.0±1.25 cm and 5.63±0.36 cm². These were significantly different (at p=0.05) from the respective values of 41.4±0.8 cm and 13.44±0.22 cm² for the control plants. Levels of total chlorophyll (per gram fresh weight of leaves) and protein (per gram dry weight of whole plant) were higher in the control plants compared with those grown in oil treated soil. Results obtained from the growth analysis showed the inhibitory effects of spent engine oil on Amaranthus hybridus L.