Biomarkers of environmental contaminants in the coastal waters of Estonia (Baltic Sea): effects on eelpouts (Zoarces viviparus)

The eastern Baltic Sea near the Estonian coast is heavily navigated by numerous cargo ships and oil tankers. Hundreds of accidents and oil spills happen yearly in this area. Yet, there is a lack of data concerning the distribution and effects of the environmental contaminants, especially polycyclic aromatic hydrocarbons (PAHs). Different parts of the Baltic Sea have different levels of contamination; therefore a wide range of monitoring stations in coastal areas in the Gulf of Finland and Gulf of Riga were chosen. The aim of the present research was to document the responses of chosen biomarkers of environmental contaminants in different unstudied areas of the Estonian coastal sea. During 2009 and 2010 we measured PAH metabolites, EROD activities, geno- and cytotoxicity, histology, parasites and other biomarkers from the eelpout (Zoarces viviparus), a resident benthic fish species. The results showed that fish from the Gulf of Riga emitted lower levels of fluorescence in fixed wavelength analyses (representing equivalents of PAH metabolites in bile and urine), and consistently, showed less geno- and cytotoxicity and parasite infection, higher liver somatic index (LSI) and a higher condition factor (CF) than fish inhabiting areas close to the Baltic proper and in the Gulf of Finland. The results point to the effect of long-range contaminant transportation, whether atmospheric or hydrodynamic, and also to the intensive shipping activity in international routes. This study fills the gap of knowledge in this area that has persisted until now. Nevertheless, more studies in this area on the different groups of contaminants are necessary, to specify the factors that are responsible for observed biological effects.     

Applying a Large,Statewide Database to the Assessment,Stressor Diagnosis,and Restoration of Stream Fish Communities

In this report, predictions of the species that were expected to occur at stream sites were generated and probable stressors to fish species that were predicted to occur but were absent were diagnosed. Predictions were generated based on the hierarchical screening method of Smith and Powell (1971, Am. Mus. Novit. 2458, 1–30), using fish abundance in conjunction with 25 environmental variables at 895 sites. The sites were sampled throughout Maryland and represent the entire range of environmental quality from severely degraded to minimally degraded. Stressor variable values that exceeded tolerance thresholds for species that were expected to occur, but were absent, were considered to be probable stressors. This method was tested for efficacy in stream site assessments and stressor diagnosis using an independent data set. Sites that were classified as degraded according to the IBI and to non-biological criteria had fewer predicted species present compared to minimally influenced sites, indicating that the proportion of predicted species present accurately represents the biological integrity of a stream site. The nine stressors that were applied to the test data set accounted for species absences in 43.7% of degraded sites. Impervious land cover was the most common stressor identified. In addition to assessing stream biological integrity and identifying stressors to fish species, this approach also provides tolerance thresholds for predicted fish species that are useful endpoints necessary to plan effective restoration of fish species in Maryland.     

A Review of the Northern Ecosystem Initiative in Arctic Canada: Facilitating Arctic Ecosystem Research Through Traditional and Novel Approaches

The Canadian Arctic is undergoing considerable social and environmental change. Anthropogenic stressors on this sensitive environment include climate change, contaminants, resource extraction, tourism and increasing human populations. The Northern Ecosystem Initiative (NEI) is a program aimed at supporting the sustainability of northern communities, and at improving our understanding of how northern ecosystems respond to these environmental stressors. A key element of the NEI is to establish partnerships between all levels of government, non-governmental environmental agencies, and northern residents. The NEI is an important source of financial support critical for social and environmental scientists as well as northern residents and their community and regional organizations. Initiated in 1998, the NEI has supported numerous northern scientific and capacity-building projects, and has evaluated the information gained from this work to refine and focus its future support to address key information gaps and northern needs.     

Integrated survey of water pollution in the Suquía River basin (Córdoba, Argentina)

We report a combined two-year seasonal monitoring of Suquía River basin using both chemical parameters and biomarkers measured in Jenynsia multidentata, aiming to correlate external levels of contaminants with the response of oxidative stress biomarkers in this fish. Identified pollution sources correspond to city sewage as well as agricultural and small industry activities downstream from Córdoba city. Physicochemical parameters integrated into a water quality index (WQI) were measured in Suquía River during dry and wet seasons. Ag, Mn, Cu, Cr, Ni, Fe, Pb and Zn were also monitored in water and sediment samples. Biomarkers include detoxication and antioxidant enzymes: catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathione reductase (GR). Enzymes showed a pollution dependent response, with increased activities in fish collected close to the sewage exit and progressive drop further downstream, matching changes in the Water Quality index. The combined use of biomarkers with water quality parameters allowed both the identification of pollution sources and the evaluation of effects of contaminants on the aquatic biota.     

Evaluating the power of surface attendance counts to detect long-term trends in populations of crevice-nesting auklets

This study assessed metals in water and different tissues of Labeo rohita and the impact of these metals on DNA and proteins as biomarkers of gills and muscles of these fish from three different polluted sites (reference or low = KW, medium = CH and high = SK) of the Indus River, Pakistan. The Mn, Pb, Cu, Zn, Hg, and Cr levels in water, gills, liver, muscles, and skin of these fish were compared with the international permissible levels. All metals except Pb and Hg in water were within the acceptable limits of drinking water. In contrast, the Mn, Hg, and Cr levels in the fish tissues were higher than their permissible limits for fish as a human food. Here, the gills contained higher metals than the other tissues. Different patterns of biomarkers were found in fish from these sites. While the gills did not show four protein bands (55, 30, 18.4, and 16.4 kDa), the muscles showed four new protein bands (100, 85, 45, and 20 kDa) for fish from the medium and high polluted sites as compared to the reference or low polluted site. The fish from the CH and SK sites of the Indus River contained low molecular weight DNA in their gills but high molecular weight DNA in their muscles when compared with the KW site. This study suggests that the proteins and DNA profiles of L. rohita could be used as biomarkers to assess the impact of potential environmental stressors such as metals on the freshwater systems.     

Combined use of rapid bioassessment protocols and sediment quality triad to assess stream quality

Physical, chemical and biological conditions at five stations on a small southeastern stream were evaluated using the Rapid Bioassessment Protocols (RBP) and the Sediment Quality Triad (SQT) to assess potential biological impacts of a municipal wastewater treatment facility (WWTF) on downstream resources. Physical habitat, benthic macroinvertebrates and fish assemblages were impaired at Stations 1 and 2 (upstream of the WWTF), suggesting that the degraded physical habitat was adversely impacting the fish and benthic populations. The SQT also demonstrated that Stations 1 and 2 were degraded, but the factors responsible for the impaired conditions were attributed to the elevated concentrations of polycylclic aromatic hydrocarbons (PAHs) and metals (Mn, Pb) in the sediments. The source of contaminants to the upper reaches of the stream appears to be storm-water runoff from the city center. Increased discharge and stabilized base flow contributed by the WWTF appeared to benefit the physically-altered stream system. Although the two assessment procedures demonstrated biological impairment at the upstream stations, the environmental factors identified as being responsible for the impairment were different: the RBP provided insight into contributions associated with the physical habitat and the SQT contributed information on contaminants and sediment quality. Both procedures are important in the identification of physical and chemical factors responsible for environmental impairment and together they provide information critical to the development of appropriate management options for mitigation.     

Assessment of the potential of the rock gunnel (Pholis gunnellus) along the Atlantic coast of Canada as a species for monitoring the reproductive impacts of contaminant exposures

Evaluating the impacts of point source discharges on fish species in estuarine environments can be challenging because of a paucity of resident species. We evaluated the biology of rock gunnel (Pholis gunnellus) at three relatively uncontaminated sites in the Bay of Fundy, along the Atlantic coast of Canada. Rock gunnel are seasonally resident (April to November) in tide pools, but little was known about their life history in Atlantic Canada or their potential for use for monitoring environmental quality. Fish were collected between April and November, and ranged from 2.46 g--15.2~g in weight and 97 mm-170 mm in length, with a maximum age of 7 years. Both males and females were similar in size, and both reached sexual maturity at a size of 5.5 g. Organ weights and condition indices of fish were stable from spring when they returned from offshore (April to May) until late summer (August to September), but fall fish (October to November) had slightly larger gonads, livers and condition indices. Rock gunnel may be a useful indicator to provide insight into local impacts of point sources over a short time period. However, they do not provide adequate information on reproductive development and performance since they are not exposed to onshore contaminants during the periods of gonadal development that have most commonly found to be sensitive to anthropogenic stressors.     

The responses of the hepatosomatic index (HSI), 7-ethoxyresorufin-O-deethylase (EROD) activity and glutathione-S-transferase (GST) activity in sea bass (Dicentrarchus labrax, Linnaeus 1758) caged at a polluted site: implications for their use in environmental risk assessment

The present study investigates the response of three hepatic biomarkers in adult sea bass (Dicentrarchus labrax, Linnaeus 1758) caged at a wastewater outlet of an oil refinery with fish caged at a pristine site used as controls. The biomarkers that were investigated were the hepatosomatic index (HSI), 7-ethoxyresorufin-O-deethylase (EROD) activity and glutathione-S-transferase (GST) activity. In addition, we have measured the levels of polycyclic aromatic hydrocarbons (PAHs) and selected heavy metals (lead, cadmium, mercury, copper and zinc) in sediment samples at the polluted site. Although the polluted site had high environmental levels of PAHs and heavy metals, there was no difference in hepatic EROD activity and HSI between fish caged at the polluted site and controls. On the other hand, GST activity was significantly lower in fish caged at the polluted site compared to controls. Our results point out that the studied biomarkers have limited use in environmental risk assessment studies, at least when caged adult sea bass is used as the sentinel species and complex toxicant mixtures are involved.     

The Use of Mytilus Galloprovincialis Acetylcholinesterase and Glutathione S-Transferases Activities as Biomarkers of Environmental Contamination Along the Northwest Portuguese Coast

With the aim of using Mytilus galloprovincialis acetylcholinesterase (AChE) and glutathione S-transferases (GST) activities as biomarkers of environmental contamination in risk assessment studies along the northwest Portuguese coast, the objective of this study was to provide background information related to: (1) baseline values of these enzymatic activities both in reference and contaminated areas and their responsiveness as indicators of exposure to environmental contaminants; (2) the importance of seasonal variations of such biomarkers in M. galloprovincialis along this area. For this, the activity of these enzymes was seasonally determined in mussels collected from four sites, including a reference and three stations with different contamination sources along the referred area. Statistically significant differences on both enzymatic activities were found among the four sampling stations, at the four sampling periods. In comparison to the reference station, lower AChE and higher GST activity values were found in mussels collected in stations potentially contaminated by pesticides and domestic/industrial effluents and in mussels collected in the vicinity of an oil refinery and an industrial/mercantile harbour, respectively. The results obtained in this work highlighted the potential suitability of these biomarkers to be used as components of environmental monitoring programs in risk assessment studies along the northwest Portuguese coast. Since a seasonal variation in both enzymatic activities was found, the possible implications of such variability in the use of these enzymes as environmental biomarkers are also discussed.     

Relationships between water mass characteristics and estimates of fish population abundance from trawl surveys

The Canadian Department of Fisheries and Oceans conducts annual bottom trawl surveys to monitor changes in the abundance of the major commercially important groundfish populations. Some of these surveys have been in operation for almost 20 yr. The estimates from these surveys often indicate rapid changes in abundance over time beyond that expected from the population dynamics of the fish. Much of this interannual change has been interpreted as variation, the magnitude of which has often made it difficult to measure anything but the most severe effects of fishing, pollution or any other intervention on the population. Recent studies have shown that some of this variation may be attributed to changes in catchability of fish due to the effects of environmental variables on fish distribution. Annual changes in abundance as estimated from such field surveys may be confounded by changes in catchability due to annual changes in environmental conditions. In this study, trawl catches of age 4 Atlantic cod (Gadus morhua) from surveys conducted during March 1979–1988 were compared with concurrent measurements of bottom salinity, temperature and depth. Large catches of age 4 cod are more likely to occur in water characterized as the intermediate cold layer defined by salinities of 32–33.5 and temperatures<5°C. This relationship also appears to be modified by depth. We further show that internnual changes in the estimated abundance from the surveys were, in a number of cases, coincident with changes in the proportion of the bottom water composed of the intermediate cold water layer. The implications that these patterns may have on interpreting trends in the estimates of abundance from trawl surveys are discussed.     

Toxicity assessment of individual ingredients of synthetic-based drilling muds (SBMs)

Synthetic-based drilling muds (SBMs) offer excellent technical characteristics while providing improved environmental performance over other drilling muds. The low acute toxicity and high biodegradability of SBMs suggest their discharge at sea would cause minimal impacts on marine ecosystems, however, chronic toxicity testing has demonstrated adverse effects of SBMs on fish health. Sparse environmental monitoring data indicate effects of SBMs on bottom invertebrates. However, no environmental toxicity assessment has been performed on fish attracted to the cutting piles. SBM formulations are mostly composed of synthetic base oils, weighting agents, and drilling additives such as emulsifiers, fluid loss agents, wetting agents, and brine. The present study aimed to evaluate the impact of exposure to individual ingredients of SBMs on fish health. To do so, a suite of biomarkers [ethoxyresorufin-O-deethylase (EROD) activity, biliary metabolites, sorbitol dehydrogenase (SDH) activity, DNA damage, and heat shock protein] have been measured in pink snapper (Pagrus auratus) exposed for 21 days to individual ingredients of SBMs. The primary emulsifier (Emul S50) followed by the fluid loss agent (LSL 50) caused the strongest biochemical responses in fish. The synthetic base oil (Rheosyn) caused the least response in juvenile fish. The results suggest that the impact of Syndrill 80:20 on fish health might be reduced by replacement of the primary emulsifier Emul S50 with an alternative ingredient of less toxicity to aquatic biota. The research provides a basis for improving the environmental performance of SBMs by reducing the environmental risk of their discharge and providing environmental managers with information regarding the potential toxicity of individual ingredients.     

Using Multiple Taxonomic Groups to Index the Ecological Condition of Lakes

Biological indicators of communitiestypically reflect a common environmental signalreflecting the general condition of the ecosystem, as well asindividual signals by indicators differentiallysensitive to particular environmental conditions. Wedescribe here a method of integrating and interpretingsuch indicators from 19 New England lakes for fivetaxonomic groups (diatoms, benthos, zooplankton, fish,and birds). Our approach provides a systematicstandardized way to integrate multiple metrics fromdifferent taxonomic groups by addressing four elementscrucial to analyzing data from multiple indicators: covariate control, re-scaling of data, standardizing the sign of responses, and dimensional reduction. We evaluated the biologicalmetrics against individual environmental stressors andagainst multivariate physicochemical metricscharacterizing general anthropogenic stress among thelakes. The method detected a response to variationin the gross environmental condition of the lakes thatwas correlated across taxa and metrics. In addition,a differential response to near shore conditions wasdemonstrated for fish. The success of the approach inthis study lends support to its general application toecological monitoring involving complex data sets.     

Monitoring fish distributions along electrofishing segments

Electrofishing is widely used to monitor fish species composition and relative abundance in streams and lakes. According to standard protocols, multiple segments are selected in a body of water to monitor population relative abundance as the ratio of total catch to total sampling effort. The standard protocol provides an assessment of fish distribution at a macrohabitat scale among segments, but not within segments. An ancillary protocol was developed for assessing fish distribution at a finer scale within electrofishing segments. The ancillary protocol was used to estimate spacing, dispersion, and association of two species along shore segments in two local reservoirs. The added information provided by the ancillary protocol may be useful for assessing fish distribution relative to fish of the same species, to fish of different species, and to environmental or habitat characteristics.     

Biological effects monitoring of the North Sea employing fish embryological data

Using the reproductive capacity of fish appears to be a suitable approach for risk assessment in the aquatic environment since fish are a typical representative thereof and in addition they are of considerable societal value. Generally the early embryonic stages are considered to be one of the most sensitive parts of a fish's life cycle. A method has been developed to use the state of health of live, naturally spawned fish embryos from plankton samples for biological effects monitoring. During the years 1985–1987 in the southern North Sea and in 1991–1992 in the whole of the North Sea fish eggs were sampled from surface waters and examined for developmental abnormalities. Elevated embryo malformation rates were detected in the plume of the major rivers Elbe and Rhine as well as along the eastern coast of England. Occurring malformations are thought to be pollution-related and may be used to define areas of environmental deterioration. The method is discussed in view of its suitability for biological effects monitoring using malformations in fish embryos as biomarkers.     

Influence of feeding procedure on biomarkers in caged rainbow trout (Oncorhynchus mykiss) used in environmental monitoring

Biomarkers in feral fish can be a useful tool for environmental monitoring of aquatic ecosystems. Drawbacks, however, are that suitable fish species are not always available and that natural variations can bias the results. An alternative strategy is to use farmed fish placed in cages. There is, however, still a risk that factors other than pollution level could have an impact on the biomarkers and the observed responses in the fish. The present study evaluates the effects of feeding procedure on biomarkers in caged fish. Two feeding rations (2% and 8% weekly feeding) have been examined for a large number of biomarkers in caged rainbow trout (Oncorhynchus mykiss). Significant effects of feeding rations were found on hepatic ethoxyresorufin-O-deethylase (EROD) and catalase activity, PAH metabolites in bile, plasma ion concentrations and the presence of immature red blood cells. The influence on EROD activity and PAH metabolites seems to be caused by elevated uptake of pollutants when feeding ratios are higher. The effects on other biochemical and physiological variables are more likely caused by stress due to insufficient feeding. According to these results, valid comparison of fish groups in environmental monitoring requires standardized feeding levels.     

Kinetic of Biomarker Responses in Juveniles of the Fish Sparus aurata Exposed to Contaminated Sediments

Sediments in the National Park of the Atlantic Islands (Galicia, Spain) were affected by the spill of the tanker Prestige (November, 2002) and still present high levels of Polycyclic aromatic hydrocarbons. The adverse effects associated with the contaminants in sediments were tested using a chronic bioassay, exposing juveniles of the fish Sparus aurata (seabream). A toxicokinetic approach is proposed to evaluate sediment quality by linking chemical and ecotoxicological data along the time. Sediment samples were physicochemically characterized and the concentration of contaminants (Polycyclic aromatic hydrocarbons – PAHs – and metals) was measured. Fishes were exposed to contaminated sediments, and samples from different tissues were collected every 15 days throughout the 60 days that lasted the experiment. A biomarker of exposure (ethoxyresorufin O-deethylase activity – EROD activity) and a biomarker of effect (histopathology) were analyzed during the exposure period. Results show a relationship between the biomarkers and the concentrations in sediments of polycyclic aromatic hydrocarbons—PAHs. Besides, the toxicokinetic approach links biomarkers response providing information about the relationship between the detoxification process and the damages observed in the different tissues. The frequency of the histological damage is highest when the EROD activity slightly decreases in accordance with the mechanism of detoxification of this enzymatic system against PAHs and other organic contaminants.     

Ecotoxicological implications of aquatic disposal of coal combustion residues in the United States: a review

We provide an overview of research related to environmental effects of disposal of coal combustion residues (CCR) in sites in the United States. Our focus is on aspects of CCR that have the potential to negatively influence aquatic organisms and thehealth of aquatic ecosystems. We identify major issues of concern, as well as areas in need of further investigation.Intentional or accidental release of CCR into aquatic systemshas generally been associated with deleterious environmental effects. A large number of metals and trace elements are presentin CCR, some of which are rapidly accumulated to high concentrations by aquatic organisms. Moreover, a variety of biological responses have been observed in organisms following exposure to and accumulation of CCR-related contaminants. In some vertebrates and invertebrates, CCR exposure has led to numerous histopathological, behavioral, and physiological (reproductive, energetic, and endocrinological) effects. Fish kills and extirpation of some fish species have been associatedwith CCR release, as have indirect effects on survival and growth of aquatic animals mediated by changes in resource abundance or quality. Recovery of CCR-impacted sites can be extremely slow due to continued cycling of contaminants withinthe system, even in sites that only received CCR effluents forshort periods of time. The literature synthesis reveals important considerations for future investigations of CCR-impacted sites. Many studies have examined biological responses to CCR with respect to Se concentrations and accumulation because of teratogenic andreproductively toxic effects known to be associated with thiselement. However, the complex mixture of metals and traceelements characteristic of CCR suggests that biologicalassessments of many CCR-contaminated habitats should examine avariety of inorganic compounds in sediments, water, and tissuesbefore causation can be linked to individual CCR components. Most evaluations of effects of CCR in aquatic environments havefocused on lentic systems and the populations of animalsoccupying them. Much less is known about CCR effects in loticsystems, in which the contaminants may be transported downstream,diluted or concentrated in downstream areas, and accumulated bymore transient species. Although some research has examinedaccumulation and effects of contaminants on terrestrial and avianspecies that visit CCR-impacted aquatic sites, more extensiveresearch is also needed in this area. Effects in terrestrial orsemiaquatic species range from accumulation and maternal transferof elements to complete recruitment failure, suggesting that CCReffects need to be examined both within and outside of theaquatic habitats into which CCR is released. Requiring specialattention are waterfowl and amphibians that use CCR-contaminatedsites during specific seasons or life stages and are highlydependent on aquatic habitat quality during those periods.Whether accidentally discharged into natural aquatic systems or present in impoundments that attract wildlife, CCR appears topresent significant risks to aquatic and semiaquatic organisms. Effects may be as subtle as changes in physiology or as drasticas extirpation of entire populations. When examined as a whole,research on responses of aquatic organisms to CCR suggests thatreducing the use of disposal methods that include an aquaticslurry phase may alleviate some environmental risks associatedwith the waste products.     

The effect of different holding conditions for environmental monitoring with caged rainbow trout (Oncorhynchus mykiss)

Biomarkers in fish can be a useful tool for environmental monitoring of aquatic ecosystems when diffuse pollution is becoming more important and new chemicals are being created continuously. There are, however, a number of drawbacks with this method. Because of environmental variability, health status of wild fish populations may differ between years, leading to unrepresentative results in long term studies. Furthermore, genetic or adaptive differences between populations complicate the interpretation of studies on different sites. The use of farmed fish, placed in cages, can reduce these problems. However, experimental conditions are likely to differ between sites. For practical reasons it may, e.g., be neccesary to use different types of caging. Here, the use of net cages and flow through tanks has been compared for a number of biomarkers. Rainbow trout (Oncorhynchus mykiss) were placed in net cages and flow through tanks in the river G?ta Alv, in western Sweden, during three different periods in 2004 and 2005. No differences between types of caging were found for any biomarker. Therefore, the results suggest that net cages and flow through tanks can be used and compared in environmental monitoring using biomarkers in caged rainbow trout. However, efforts should be taken to reduce differences in experimental conditions, e.g., light intensity and feeding levels.     

Use of Small Forage Fish for Regional Streams Wildlife Risk Assessment: Relative Bioaccumulation of Contaminants

Selection of fish species for sampling to assess extent and potential effects of fish tissue contaminants is a criticalconsideration in the design of regional probability-designsurveys. The ideal species would be ubiquitous, bioaccumulatetoxic chemicals, and be prey of consumers of concern(piscivorous wildlife, humans). In first to third order streams,small short-lived forage fish (minnows (F. Cyprinidae), darters (F. Percidae), and sculpins (F. Cottidae), are more frequently found and therefore likely to be prey to more species ofwildlife than those of sportfish whose adults grow to a largesize (suckers (F. Catostomidae), trout (F. Salmonidae), bass, andsunfish (F. Centrarchidae), and carp). Targeting smaller foragefish should also produce a larger number of individuals persample on average than may be achieved with the larger species.An analysis of fish collected in 1993 and 1994 as part of theMid-Atlantic Highlands Assessment (MAHA) showed that, asexpected, forage fish were more ubiquitous than sportfishspecies. Analysis also revealed that, on a regional basis, forage fish bioaccumulated comparable levels to sportfish, ofsome widely occurring contaminants such as DDT, MeHg, and PCBs.Results indicated that smaller forage fish can be used asindicator species for a regional assessment for mostcontaminants (Zn being the one clear exception), that was notsignificantly different from one based on the larger species.Forage fish may therefore be an excellent choice as indicatorspecies for regional streams ecological risk assessment studies.     

Reproductive and Physiological Effects of Environmental Contaminants in Fish-Eating Birds of the Great Lakes: A Review of Historical Trends

During the 1950s and 1960s, reproductive failures and population declines were observed in fish-eating birds such as gulls, terns, cormorants, herons, and eagles in the Great Lakes. DDE-induced eggshell thinning contributed to these declines, but other factors such as embryo toxicity also were implicated. With reduced releases of many pollutants, reproduction recovered in some species. However, biomonitoring during the 1980s and 1990s indicates continuing effects at highly contaminated sites. Improved analytical techniques and bioassays have allowed the assessment of the total dioxin-like toxicity of complex mixtures of organochlorines (TCDD-equivalents). Developmental defects such as embryo mortality, deformities, and edema have been associated with dioxin-like PCBs in several avian species. Improved biochemical techniques have allowed the measurement of biomarkers that detect physiological alterations associated with contaminants. Specific biomarkers studied in Great Lakes birds include cytochrome P-450 monooxygenases, highly carboxylated porphyrins, thyroxine, vitamin A, and immune function. Reproductive and physiological alterations are associated with population-level effects in Caspian terns and bald eagles that feed on highly contaminated fish. Biomonitoring using biomarkers and population-level measures in fish-eating birds will continue to be important for assessing the effects of contaminants on the Great Lakes ecosystem.