Protected‐Area Boundaries as Filters of Plant Invasions

Abstract: Human land uses surrounding protected areas provide propagules for colonization of these areas by non‐native species, and corridors between protected‐area networks and drainage systems of rivers provide pathways for long‐distance dispersal of non‐native species. Nevertheless, the influence of protected‐area boundaries on colonization of protected areas by invasive non‐native species is unknown. We drew on a spatially explicit data set of more than 27,000 non‐native plant presence records for South Africa's Kruger National Park to examine the role of boundaries in preventing colonization of protected areas by non‐native species. The number of records of non‐native invasive plants declined rapidly beyond 1500 m inside the park; thus, we believe that the park boundary limited the spread of non‐native plants. The number of non‐native invasive plants inside the park was a function of the amount of water runoff, density of major roads, and the presence of natural vegetation outside the park. Of the types of human‐induced disturbance, only the density of major roads outside the protected area significantly increased the number of non‐native plant records. Our findings suggest that the probability of incursion of invasive plants into protected areas can be quantified reliably.     

Differing Effects of Cattle Grazing on Native and Alien Plants

Abstract:   Habitat managers use cattle grazing to reduce alien plant cover and promote native species in California grasslands and elsewhere in the western United States. We tested the effectiveness of grazing as a restoration method by examining the effects of herbivory on native and alien plants. At Carrizo Plain National Monument, California, we surveyed native and alien species cover in adjacent grazed and ungrazed areas. We also established experimental plots in which plants were clipped or mulch (dead biomass) was removed. In addition, we clipped plants grown in pots and plants in the field that grew with and without competitors. Native species were negatively affected by clipping in 1999, 2000, and 2001, whereas alien species were unaffected. In the experimental field plots, the European annual forb Erodium cicutarium compensated in growth and reproduction following simulated herbivory. In contrast, growth and reproduction of the native perennial bunchgrass Poa secunda were reduced 1 year after clipping. In pots, E. cicutarium overcompensated and grasses undercompensated. In the field, European grasses were unaffected by the removal of competitors. It is unclear by what mechanism E. cicutarium was able to compensate, but the ability may be related to its basal rosette growth form and indeterminately growing inflorescences. The native California grassland community assembled in the absence of grazing herds, whereas invasive European species have been exposed to grazing for centuries. It may be that these invaders have adaptations that better enable them to recover from grazing. In the grassland we studied, the strategy of livestock grazing for restoration is counterproductive. It harms native species and promotes alien plant growth.     

Terrestrial Reserve Networks Do Not Adequately Represent Aquatic Ecosystems

Abstract: Protected areas are a cornerstone of conservation and have been designed largely around terrestrial features. Freshwater species and ecosystems are highly imperiled, but the effectiveness of existing protected areas in representing freshwater features is poorly known. Using the inland waters of Michigan as a test case, we quantified the coverage of four key freshwater features (wetlands, riparian zones, groundwater recharge, rare species) within conservation lands and compared these with representation of terrestrial features. Wetlands were included within protected areas more often than expected by chance, but riparian zones were underrepresented across all (GAP 1–3) protected lands, particularly for headwater streams and large rivers. Nevertheless, within strictly protected lands (GAP 1–2), riparian zones were highly represented because of the contribution of the national Wild and Scenic Rivers Program. Representation of areas of groundwater recharge was generally proportional to area of the reserve network within watersheds, although a recharge hotspot associated with some of Michigan's most valued rivers is almost entirely unprotected. Species representation in protected areas differed significantly among obligate aquatic, wetland, and terrestrial species, with representation generally highest for terrestrial species and lowest for aquatic species. Our results illustrate the need to further evaluate and address the representation of freshwater features within protected areas and the value of broadening gap analysis and other protected‐areas assessments to include key ecosystem processes that are requisite to long‐term conservation of species and ecosystems. We conclude that terrestrially oriented protected‐area networks provide a weak safety net for aquatic features, which means complementary planning and management for both freshwater and terrestrial conservation targets is needed.     

The Control of Biological Invasions in the World's Oceans

Abstract: The introduction of alien, or nonindigenous, animals and plants has been identified by scientists and policy makers as a major threat to biodiversity in marine ecosystems. Although government agencies have struggled to control alien species on land and freshwater for decades with mixed success, the control of alien marine species is in its infancy. Prevention of introduction and establishment must be the first priority, but many populations of alien marine species are already well established worldwide. National and international policies leave loopholes for additional invasions to occur and provide only general guidance on how to control alien species once they are established. To address this issue, a multinational group of 25 scientists and attorneys convened in 1998 to examine options for controlling established populations of alien marine species. The discussions resulted in a framework for control of alien marine species to provide decision-making guidance to policymakers, managers, scientists, and other stakeholders. The framework consists of seven basic steps: (1) establish the nature and magnitude of the problem, (2) set objectives, (3) consider the full range of alternatives, (4) determine risk, (5) reduce risk, (6) assess benefits versus risks, and ( 7) monitor the situation. This framework can provide guidance for control efforts under the existing patchwork of national laws and can help provide a foundation for international cooperation.     

Pricing Policy for Tourism in Protected Areas: Lessons from Komodo National Park, Indonesia

Abstract: Protected areas are under increasing pressure to provide economic justification for their existence, particularly in developing countries where demand for land and natural resources is high. Nature-based tourism offers a mechanism to generate substantial benefits from protected areas for both governments and local communities, and ecotourism is increasingly promoted as a sustainable use of protected areas. The extent to which ecotourism offsets the costs of a protected area has rarely been examined. We used financial data from Komodo National Park, Indonesia, and a willingness-to-pay questionnaire of independent visitors to (1) examine the financial contribution of tourism in offsetting the costs of tourism and wider management and (2) assess the effect of hypothetical fee increases on park revenues, visitation patterns, and local economies. Although only 6.9% of park management costs were recovered, visitors were willing to pay over 10 times the current entrance fee, indicating a substantial potential for increased revenue. The potential negative effect of large fee increases on visitor numbers and the resultant effect on local economic benefits from tourism may limit the extent to which greater financial benefits from Komodo National Park (KNP) can be realized. Our results suggest that a moderate, tiered increase in entrance fees is most appropriate, and that partial revenue retention by KNP would help demonstrate the conservation value of tourism to both visitors and managers and has the potential to increase visitors' willingness to pay.     

Invasibility of Species-Rich Communities in Riparian Zones

Invasibility of riparian plant communities was estimated by the percentage of alien species found along the Adour River (Southwest France) and along Lockout Creek, McKenzie River, and Willamette River (Central Cascades, Oregon, U.S.A.). At the patch scale, the invasibilities of riparian plant communities were compared between one exceptionally rich site of the Adour River and patches selected in the Hoh and Dungeness watersheds (Olympic Peninsula, Washington, U.S.A.). Alien species represented 24% of 1396 species for the Adour and 30% of 851 species for the McKenzie. They represented 24% of 148 species for the Hoh drainage and 28% of 200 species for the Dungeness drainage. Similar trends were found along the Adour River and along the McKenzie River for changes in total number of species per site and in percentages of alien species per site. These trends may be related to the intermediate disturbance regimes and to the physical structure of the riparian corridors. Climatic and human factors are also involved in these longitudinal changes. Positive linear relationships were found between the total number of species and the percentage of aliens observed in each site. At the patch scale, most of the sampled communities contained alien species. Although mature vegetative patches appeared to be invasible, young communities contained more alien species than older ones. For entire corridors, a positive linear relationship was found between total species richness and percentage of alien species in each patch type for the richest site of the Adour River. This may be partially explained by landscape features considered in a successional context. We suggest the use of empirical rules, and stress the importance of riparian systems for monitoring the conservation of local and regional species pools are suggested.     

Rank aggregation of local expert knowledge for conservation planning of the critically endangered saola

There has been much recent interest in using local knowledge and expert opinion for conservation planning, particularly for hard‐to‐detect species. Although it is possible to ask for direct estimation of quantities such as population size, relative abundance is easier to estimate. However, an expert's knowledge is often geographically restricted relative to the area of interest. Combining (or aggregating) experts’ assessments of relative abundance is difficult when each expert only knows a part of the area of interest. We used Google's PageRank algorithm to aggregate ranked abundance scores elicited from local experts through a rapid rural‐appraisal method. We applied this technique to conservation planning for the saola (Pseudoryx nghetinhensis), a poorly known bovid. Near a priority landscape for the species, composed of 3 contiguous protected areas, we asked groups of local people to indicate relative abundances of saola and other species by placing beans on community maps. For each village, we used this information to rank areas within the knowledge area of that village for saola abundance. We used simulations to compare alternative methods to aggregate the rankings from the different villages. The best‐performing method was then used to produce a single map of relative abundance across the entire landscape, an area larger than that known to any one village. This map has informed prioritization of surveys and conservation action in the continued absence of direct information about the saola.     

A comparative analysis of alien plant species along the Romanian Black Sea coastal area. The role of harbours

Previous studies have found a higher proportion of alien plant species along the coastal area of the Black Sea. The goals of the present study were to assess the role of two harbours as gateways and reservoirs for alien plant species, to compare the structure and invasion pattern of the alien plants recorded there, and test methods useful for effective monitoring programs. We inventoried 12 sites along the western Black Sea coast from the harbour of Sulina in the north to Cape Kaliakra in the south. Each site was visited at least three times each. A more intensive survey was done in the two harbours targeted by our study: Constanţa and Sulina. The proportion of neophytes was higher in the harbours (representing about one third of the total plant species) and lower in coastal protected areas (with an average proportion of 6.7%). Species accumulation curves and estimators of species richness indicated that while the plant inventory was not complete, invasive alien species (IAS) were adequately inventoried. Harbours act not only as gateways for IAS but also as reservoirs, facilitating their acclimatization and naturalization. The use of species accumulation curves and estimators of species richness are useful tools in designing and evaluating simple monitoring programs based on repeated inventories. Our study has stressed the importance of monitoring not only coastal waters but also green areas in harbours for the early detection of IAS.     

The human footprint in the west: a large-scale analysis of anthropogenic impacts

Anthropogenic features such as urbanization, roads, and power lines, are increasing in western United States landscapes in response to rapidly growing human populations. However, their spatial effects have not been evaluated. Our goal was to model the human footprint across the western United States. We first delineated the actual area occupied by anthropogenic features, the physical effect area. Next, we developed the human footprint model based on the ecological effect area, the zone influenced by features beyond their physical presence, by combining seven input models: three models quantified top-down anthropogenic influences of synanthropic predators (avian predators, domestic dog and cat presence risk), and four models quantified bottom-up anthropogenic influences on habitat (invasion of exotic plants, human-caused fires, energy extraction, and anthropogenic wildland fragmentation). Using independent bird population data, we found bird abundance of four synanthropic species to correlate positively with human footprint intensity and negatively for three of the six species influenced by habitat fragmentation. We then evaluated the extent of the human footprint in relation to terrestrial (ecoregions) and aquatic systems (major rivers and lakes), regional management and conservation status, physical environment, and temporal changes in human actions. The physical effect area of anthropogenic features covered 13% of the western United States with agricultural land (9.8%) being most dominant. High-intensity human footprint areas (class 8-10) overlapped highly productive low-elevation private landholdings and covered 7% of the western United States compared to 48% for low-intensity areas (class 1-3), which were confined to low-productivity high-elevation federal landholdings. Areas within 1 km of rivers were more affected by the human footprint compared to lakes. Percentage human population growth was higher in low-intensity human footprint areas. The disproportional regional effects of the human footprint on landscapes in the western United States create a challenge to management of ecosystems and wildlife populations. Using footprint models, managers can plan land use actions, develop restoration scenarios, and identify areas of high conservation value at local landscapes within a regional context. Moreover, human footprint models serve as a tool to stratify landscapes for studies investigating floral and faunal response to human disturbance intensity gradients.     

Invasional meltdown: invader-invader mutualism facilitates a secondary invasion

In multiply invaded ecosystems, introduced species should interact with each other as well as with native species. Invader-invader interactions may affect the success of further invaders by altering attributes of recipient communities and propagule pressure. The invasional meltdown hypothesis (IMH) posits that positive interactions among invaders initiate positive population-level feedback that intensifies impacts and promotes secondary invasions. IMH remains controversial: few studies show feedback between invaders that amplifies their effects, and none yet demonstrate facilitation of entry and spread of secondary invaders. Our results show that supercolonies of an alien ant, promoted by mutualism with introduced honeydew-secreting scale insects, permitted invasion by an exotic land snail on Christmas Island, Indian Ocean. Modeling of land snail spread over 750 sites across 135 km2 over seven years showed that the probability of land snail invasion was facilitated 253-fold in ant supercolonies but impeded in intact forest where predaceous native land crabs remained abundant. Land snail occurrence at neighboring sites, a measure of propagule pressure, also promoted land snail spread. Site comparisons and experiments revealed that ant supercolonies, by killing land crabs but not land snails, disrupted biotic resistance and provided enemy-free space. Predation pressure on land snails was lower (28.6%), survival 115 times longer, and abundance 20-fold greater in supercolonies than in intact forest. Whole-ecosystem suppression of supercolonies reversed the probability of land snail invasion by allowing recolonization of land crabs; land snails were much less likely (0.79%) to invade sites where supercolonies were suppressed than where they remained intact. Our results provide strong empirical evidence for IMH by demonstrating that mutualism between invaders reconfigures key interactions in the recipient community. This facilitates entry of secondary invaders and elevates propagule pressure, propagating their spread at the whole-ecosystem level. We show that identification and management of key facilitative interactions in invaded ecosystems can be used to reverse impacts and restore resistance to further invasions.     

Conservation Planning in Forest Landscapes of Fennoscandia and an Approach to the Challenge of Countdown 2010

Abstract:  Effective management of biodiversity in production landscapes requires a conservation approach that acknowledges the complexity of ecological and cultural systems in time and space. Fennoscandia has experienced major loss of forest biodiversity caused by intensive forestry. Therefore, the Countdown 2010 initiative to halt the loss of biodiversity in Europe is highly relevant to forest management in this part of the continent. As a contribution to meeting the challenge posed by Countdown 2010, we developed a spatially explicit conservation-planning exercise that used regional knowledge on forest biodiversity to provide support for managers attempting to halt further loss of biological diversity in the region. We used current data on the distribution of 169 species (including 68 red-listed species) representing different forest habitats and ecologies along with forest data within the frame of modern conservation software to devise a map of priority areas for conservation. The top 10% of priority areas contained over 75% of red-listed species locations and 41% of existing protected forest areas, but only 58% of these top priorities overlapped with core areas identified previously in a regional strategy that used more qualitative methods. We argue for aggregating present and future habitat value of single management units to landscape and regional scales to identify potential bottlenecks in habitat availability linked to landscape dynamics. To address the challenge of Countdown 2010, a general framework for forest conservation planning in Fennoscandia needs to cover different conservation issues, tools, and data needs.     

Potential Interference Between a Threatened Endemic Thistle and an Invasive Nonnative Plant

Although the establishment of nonnative plants is recognized as a threat to native ecosystems, there are few documented examples of an invasive species directly influencing a rare native plant. The Eurasian biennial Dipsacus sylvestris (teasel) is invading the central New Mexico habitat of Cirsium vinaceum , an endemic thistle that is federally listed as threatened. We documented changes in teasel distribution and abundance between 1989 and 1993 that suggest the potential for direct interactions with the native thistle. We then compared habitat characteristics, germination behavior, and performance in greenhouse and field competition trials to evaluate the potential outcome of interference between these two species. There were no significant differences in measured habitat characteristics between sites supporting C. vinaceum and those with D. sylvestris. Dipsacus was better able to germinate in low light than the thistle, suggesting that D. sylvestris might invade C. vinaceum populations but that thistle recruitment would be unlikely in dense stands of the nonnative plants. In the greenhouse growth of C. vinaceum rosettes was significantly reduced by the presence of Dipsacus , but the invader was unaffected by the thistle; results of a short-term field experiment were equivocal but suggestive of interference between the two. We suggest criteria for managers to use in determining whether invading species pose problems for specific rare native taxa, and we discuss the constraints on experimental work where protected taxa are involved.     

Boosting freshwater fish conservation with high-resolution distribution mapping across a large territory

The lack of high-resolution distribution maps for freshwater species across large extents fundamentally challenges biodiversity conservation worldwide. We devised a simple framework to delineate the distributions of freshwater fishes in a high-resolution drainage map based on stacked species distribution models and expert information. We applied this framework to the entire Chinese freshwater fish fauna (>1600 species) to examine high-resolution biodiversity patterns and reveal potential conflicts between freshwater biodiversity and anthropogenic disturbances. The correlations between spatial patterns of biodiversity facets (species richness, endemicity, and phylogenetic diversity) were all significant (r = 0.43–0.98, p < 0.001). Areas with high values of different biodiversity facets overlapped with anthropogenic disturbances. Existing protected areas (PAs), covering 22% of China's territory, protected 25–29% of fish habitats, 16–23% of species, and 30–31% of priority conservation areas. Moreover, 6–21% of the species were completely unprotected. These results suggest the need for extending the network of PAs to ensure the conservation of China's freshwater fishes and the goods and services they provide. Specifically, middle to low reaches of large rivers and their associated lakes from northeast to southwest China hosted the most diverse species assemblages and thus should be the target of future expansions of the network of PAs. More generally, our framework, which can be used to draw high-resolution freshwater biodiversity maps combining species occurrence data and expert knowledge on species distribution, provides an efficient way to design PAs regardless of the ecosystem, taxonomic group, or region considered.     

Spatial variation in caddisfly grazing regimes within a northern California watershed

Ecologists seek better understanding of why species interactions change across space and time in natural communities. In streams, species effects on resources and community structure may change as physical characteristics of the stream environment change along drainage networks. We examined spatial and seasonal effects of armored grazers using a small-scale exclusion experiment that was replicated in streams of different drainage areas. Effects of grazing varied with stream size and were related to variation in grazer abundance and phenology. We identified three distinct grazing regimes and a stream size (drainage area [DA]) threshold corresponding to a shift from one to two functional trophic levels. In streams with DA < 1 km2, armored grazers did not reduce biomass of algal biofilms. In slightly larger streams (2-3 km2 DA), the armored grazer guild was dominated by bivoltine Glossosoma. These caddisflies persisted and limited algal biofilms throughout the summer in one of these streams. In the largest tributaries (DA > 10 km2), the grazer guild was dominated by univoltine caddisflies, and grazing limited algal biofilms in early summer, but not late summer, after caddisflies pupated. Drainage area is a useful predictor of spatial transitions in food web interactions within and among watersheds. Quantifying the drainage area threshold at which interactions change in catchments with differing geology, vegetation, hydrology, climate, land use, or species pools should help build the understanding we need to forecast ecological responses to environmental change.     

Abiotic constraints eclipse biotic resistance in determining invasibility along experimental vernal pool gradients.

Effective management of invasive species requires that we understand the mechanisms determining community invasibility. Successful invaders must tolerate abiotic conditions and overcome resistance from native species in invaded habitats. Biotic resistance to invasions may reflect the diversity, abundance, or identity of species in a community. Few studies, however, have examined the relative importance of abiotic and biotic factors determining community invasibility. In a greenhouse experiment, we simulated the abiotic and biotic gradients typically found in vernal pools to better understand their impacts on invasibility. Specifically, we invaded plant communities differing in richness, identity, and abundance of native plants (the "plant neighborhood") and depth of inundation to measure their effects on growth, reproduction, and survival of five exotic plant species. Inundation reduced growth, reproduction, and survival of the five exotic species more than did plant neighborhood. Inundation reduced survival of three species and growth and reproduction of all five species. Neighboring plants reduced growth and reproduction of three species but generally did not affect survival. Brassica rapa, Centaurea solstitialis, and Vicia villosa all suffered high mortality due to inundation but were generally unaffected by neighboring plants. In contrast, Hordeum marinum and Lolium multiflorum, whose survival was unaffected by inundation, were more impacted by neighboring plants. However, the four measures describing plant neighborhood differed in their effects. Neighbor abundance impacted growth and reproduction more than did neighbor richness or identity, with growth and reproduction generally decreasing with increasing density and mass of neighbors. Collectively, these results suggest that abiotic constraints play the dominant role in determining invasibility along vernal pool and similar gradients. By reducing survival, abiotic constraints allow only species with the appropriate morphological and physiological traits to invade. In contrast, biotic resistance reduces invasibility only in more benign environments and is best predicted by the abundance, rather than diversity, of neighbors. These results suggest that stressful environments are not likely to be invaded by most exotic species. However, species, such as H. marinum, that are able to invade these habitats require careful management, especially since these environments often harbor rare species and communities.     

中国生物多样性重点保护区评价标准探讨

从基于景观大尺度的评价和基于保护区域小尺度的评价2个层次,综述了国内外研究者在生物多样性重点区域评价方面开展的工作.尝试构建一般标准和鉴定标准的双重中国生物多样性重点保护区评价标准.其中,一般标准是作为重点保护区的基本条件,必须全部满足,而鉴定标准是依据相应指标进行判断,凡具有任意1项或多项指标的区域可被确认为重点保护区.并就指标体系和评价标准的具体内涵作了阐述.     

Predicting abundance of desert riparian birds: validation and calibration of the Effective Area Model.

Reliable prediction of the effects of landscape change on species abundance is critical to land managers who must make frequent, rapid decisions with long-term consequences. However, due to inherent temporal and spatial variability in ecological systems, previous attempts to predict species abundance in novel locations and/or time frames have been largely unsuccessful. The Effective Area Model (EAM) uses change in habitat composition and geometry coupled with response of animals to habitat edges to predict change in species abundance at a landscape scale. Our research goals were to validate EAM abundance predictions in new locations and to develop a calibration framework that enables absolute abundance predictions in novel regions or time frames. For model validation, we compared the EAM to a null model excluding edge effects in terms of accurate prediction of species abundance. The EAM outperformed the null model for 83.3% of species (N=12) for which it was possible to discern a difference when considering 50 validation sites. Likewise, the EAM outperformed the null model when considering subsets of validation sites categorized on the basis of four variables (isolation, presence of water, region, and focal habitat). Additionally, we explored a framework for producing calibrated models to decrease prediction error given inherent temporal and spatial variability in abundance. We calibrated the EAM to new locations using linear regression between observed and predicted abundance with and without additional habitat covariates. We found that model adjustments for unexplained variability in time and space, as well as variability that can be explained by incorporating additional covariates, improved EAM predictions. Calibrated EAM abundance estimates with additional site-level variables explained a significant amount of variability (P < 0.05) in observed abundance for 17 of 20 species, with R2 values >25% for 12 species, >48% for six species, and >60% for four species when considering all predictive models. The calibration framework described in this paper can be used to predict absolute abundance in sites different from those in which data were collected if the target population of sites to which one would like to statistically infer is sampled in a probabilistic way.     

Value of protected areas to avian persistence across 20 years of climate and land-use change

Establishing protected areas, where human activities and land cover changes are restricted, is among the most widely used strategies for biodiversity conservation. This practice is based on the assumption that protected areas buffer species from processes that drive extinction. However, protected areas can maintain biodiversity in the face of climate change and subsequent shifts in distributions have been questioned. We evaluated the degree to which protected areas influenced colonization and extinction patterns of 97 avian species over 20 years in the northeastern United States. We fitted single-visit dynamic occupancy models to data from Breeding Bird Atlases to quantify the magnitude of the effect of drivers of local colonization and extinction (e.g., climate, land cover, and amount of protected area) in heterogeneous landscapes that varied in the amount of area under protection. Colonization and extinction probabilities improved as the amount of protected area increased, but these effects were conditional on landscape context and species characteristics. In this forest-dominated region, benefits of additional land protection were greatest when both forest cover in a grid square and amount of protected area in neighboring grid squares were low. Effects did not vary with species’ migratory habit or conservation status. Increasing the amounts of land protection benefitted the range margins species but not the core range species. The greatest improvements in colonization and extinction rates accrued for forest birds relative to open-habitat or generalist species. Overall, protected areas stemmed extinction more than they promoted colonization. Our results indicate that land protection remains a viable conservation strategy despite changing habitat and climate, as protected areas both reduce the risk of local extinction and facilitate movement into new areas. Our findings suggest conservation in the face of climate change favors creation of new protected areas over enlarging existing ones as the optimal strategy to reduce extinction and provide stepping stones for the greatest number of species.     

Indigenous Uses, Population Density, and Conservation of Threatened Medicinal Plants in Protected Areas of the Indian Himalayas

Abstract:  For 10 years I monitored the population density of threatened medicinal plant species in seven protected areas in the Indian Himalayas. I also documented the indigenous uses of threatened medicinal plants through interviews with 138 herbal healers (83 Tibetan healers and 55 Ayurvedic healers) residing in the buffer zone villages of these protected areas. To assess the population status of threatened medicinal plant species, I sampled the 10 major habitat types in the protected areas. In all, I found 60 threatened medicinal plant species during the study period, of which 54 species occurred in the sampling plots. Twenty-two percent of threatened medicinal plant species were critically endangered, 16% were endangered, and 27% were vulnerable. Thirty-two threatened medicinal plant species were endemic to the Himalayan region. The density of threatened medicinal plant species varied with protected areas. The Valley of Flowers protected area had the highest number of threatened medicinal plant species. The "moist" habitat type was richest in these species among all 10 habitat types sampled. Arnebia euchroma (Royle ex Benth.) Johnston and Ephedra gerardiana Wall. ex Stapf. were the most common threatened medicinal plant species. The indigenous groups of healers used these threatened species in curing about 45 different ailments. Based on my findings, I believe that to ensure the long-term sustainability of threatened medicinal plants, medicinal-plant conservation areas should be established.