Is tree growth reduction related to direct foliar injuries or soil chemistry modifications?

In the context of intense emissions causing atmospheric pollution, tree growth reductions could be related to soil chemistry modifications or direct foliar injuries. To verify these hypotheses, mineral soils were sampled in an area (Murdochville, Canada) where previous studies had demonstrated that tree growth was impacted by smelter emissions and that forest floor lead concentrations could be used as a proxy for atmospheric pollutant depositions. Samples were analysed for Al, Pb (concentrations and isotope ratios), basic cations (Ca, K, P, and Mg) and Zr. Mass balance calculations were performed on soil profiles to assess vertical migration of elements. Pb concentrations in litter diminished gradually with distance from the smelter. The Pb isotope ratios in these organic soil layers were close to those measured in the Murdochville ores. These patterns were not encountered in mineral soil layers. Pb isotope ratios in these layers were close to those measured in uncontaminated geological materials, and Pb concentrations and basic cation depletions were not related to the proximity of the smelter. Growth reduction was closely associated with litter Pb concentrations, which were used as a proxy for atmospheric deposition, but was not correlated with any elemental concentration or cation depletion measured in mineral soil layers. Our overall results suggest that trees responded mainly to direct atmospheric emissions, which caused foliar damage, rather than to soil chemistry modifications.     

Heavy metal pollution and forest health in the Ukrainian Carpathians

The Ukrainian Carpathians are characterized by high air pollution caused by emissions from numerous industries. We have been monitoring the state of forests in this region since 1989. The highest levels of tree defoliation (>30%) are found close to industrial emission sources and in the upper mountain forests of the Ivano-Frankivsk and Chernivtsi regions. This is caused by a combination of strong anthropogenic influences (pollution, illegal uses, recreation) as well as poor site and climatic conditions. In the Ivano-Frankivsk region, Cd and Mo accumulate in forest soils; Cr, Mo and Zn soil concentrations are higher than their limit levels; and Pb concentrations exceed toxic levels close to industrial areas (10% of the region territory). Local background levels of heavy metals are greatly exceeded in snow close to industrial regions. Analysis of correlation matrices shows that the chemical elements Ba, Cd, Co, Cr, Cu, Mo, Ni, Pb, V and Zn occur at pollution levels in natural ecosystems in the Ukrainian Carpathians. Maximum concentrations of toxic elements occur in the oak forest zone; the most industrially developed area of the region. Toxic heavy metals in the Ukrainian Carpathians forests enter with precipitation and dustfall, then become fixed in soil and accumulate in leaves, needles of vascular plants and mosses. Concentrations of these metals decrease with altitude: highest in the oak forests, less in beech, and lowest in the spruce forest zones. However, some chemical elements have the highest concentrations in spruce forests; V in needles, As in snow, and Ba and Al in soils.     

Effects of heavy metal contamination on macronutrient availability and acidification parameters in forest soil in the vicinity of the Harjavalta Cu-Ni smelter, SW Finland

Four experiments were established (1992) in Scots pine stands at distances of 0.5, 2, 4 and 8 km along a line running to the SE of the Cu-Ni smelter at Harjavalta, SW Finland, in order to investigate the effects of Cu and Ni emissions on macronutrient availability and estimates of cation exchange capacity (CEC) and base saturation (BS). The accumulation of Cu and Ni (total, exchangeable) in forest soil close to the smelter has resulted in a deficit of base cations (exchangeable Ca, Mg, K and BS) in the organic layer caused by inhibition of mineralisation and the displacement of base cations from cation exchange sites by Cu and Ni cations. No signs of soil acidification were found in the topmost layers of the soil measured as a change in pH, exchangeable acidity and Al. The determination of CEC by the summation method in heavy-metal polluted forest soils is not recommended unless heavy metal cations are also included in the calculations.     

Spatio-temporal variation of element accumulation by Moehringia trinervia in a polluted forest ecosystem (South Poland)

The aim of this study was to identify factors affecting accumulation of elements by Moehringia trinervia (L.) Clairv. in a forest ecosystem impacted by long-term inflow of air pollution. The concentrations of N, S, Ca, K, Mg, Cd, Cu, Fe, Pb and Zn were determined in plants collected in 1999. In addition, Cd, Cu, Pb and Zn content was compared with measurements from 1984. Soil properties and bulk precipitation chemistry were used as explaining variables. The amount of heavy metals accumulated by M. trinervia depended on the magnitude of industrial emissions, but only Cu and Pb concentrations were clearly related to the distance from the pollution sources. The spatial distribution of Fe content was shaped by habitat conditions, whereas the distributions of Cd and Zn content were unexplained. Among the macronutrients, only Mg uptake was affected by environmental properties: it was lower under high concentrations of soil Cd and Zn.     

Sulphur dioxide adsorption in Scots pine canopies exposed to high ammonia emissions near a Cu-Ni smelter in SW Finland

Since 1994 the nickel-processing plant at the Cu-Ni smelter at Harjavalta, south-west Finland, has emitted considerable amounts of NH(3) into the atmosphere. The effects of NH(3) emissions on nitrogen and sulphur deposition in throughfall and the foliar nutrient status were investigated in a Scots pine stand at 0.5 km distance. Bulk deposition, stand throughfall and percolation water (20 cm depth) samples were collected at 4-week intervals during 1992-1998. pH and the Ca, Mg, K, NH(4) and SO(4) concentrations were determined on the samples. NH(3) emissions have strongly increased the scavenging of SO(2) from the air in the pine stand, and the increased levels of N and S deposition were clearly evident as increased foliar N and S concentrations and larger needle size. The increased input of SO(4) into the forest floor was not associated with an increase in the leaching of Ca and Mg from the surface soil layers.     

Total vs. internal element concentrations in Scots pine needles along a sulphur and metal pollution gradient

Analysis of foliar elements is a commonly used method for studying tree nutrition and for monitoring the impacts of air pollutants on forest ecosystems. Interpretations based on the results of foliar element analysis may, however, be different in nutrition vs. monitoring studies. We studied the impacts of severe sulphur and metal (mainly Cu and Ni) pollution on the element concentrations (Al, Ca, Cu, Fe, K, Mg, Mn, Ni, P, Pb, S and Zn) in Scots pine (Pinus sylvestris L.) foliage along an airborne sulphur and metal pollution gradient. Emphasis was put on determining the contribution of air-borne particles that have accumulated on needle surfaces to the total foliage concentrations. A comparison of two soil extraction methods was carried out in order to obtain a reliable estimate of plant-available element concentrations in the soil. Element concentrations in the soil showed only a weak relationship with internal foliar concentrations. There were no clear differences between the total and internal needle S concentrations along the gradient, whereas at the plot closest to the metal smelter complex the total Cu concentrations in the youngest needles were 1.3-fold and Ni concentrations over 1.6-fold higher than the internal needle concentrations. Chloroform-extracted surface wax was found to have Ni and Cu concentrations of as high as 3000 and 600 microg/g of wax, respectively. Our results suggest that bioindicator studies (e.g. monitoring studies) may require different foliar analysis techniques from those used in studies on the nutritional status of trees.     

Soil acidity reconstruction based on tree ring information of a dominant species Abies fabri in the subalpine forest ecosystems in southwest China

To assess the suitability of dendrochemistry as an indicator of soil acidification, soil chemistry and tree ring information of Abies fabri were measured at two distinct sites (severe acid deposition site-Emei Mountain and clean site-Gongga Mountain) of the subalpine forest ecosystems of western Sichuan, southwest China. The actual soil acidity (pH) was significantly correlated with some of the recent xylem cation (Ca, Mg, Mn, Al, Sr and Ba) concentrations and their molar ratios. Xylem Ca/Mg and Ca/Mn of A. fabri were ultimately selected to reconstruct the historical changes of soil pH in Emei Mountain and Gongga Mountain, respectively. The validity of those rebuild was also verified to a certain extent. We conclude that xylem cation molar ratios of A. fabri were superior to the single cation concentrations in soil acidity rebuild at the study sites due to normalizing for concentration fluctuations.     

Simulated Effects of Acidic Solutions on Element Dynamics in Monsoon Evergreen Broad-leaved Forest at Dinghushan,China - Part 1: Dynamics of K,Na, Ca,Mg and P (7 pp)

Background, Aim and Scope Acid deposition has become a concern in south China in recent years. This phenomenon has increased to a dramatic extent with the large use of cars and coal- fueled power plants. As a consequence, soils are becoming acidified and their element dynamics will change. A decrease in the nutrient availability will lead to slower plant growth and maybe to a change in the forest type with current species being replaced by new ones with less nutrient requirements. Because of these reasons, it is important to understand how the dynamics of elements will change and what mechanism is part of the process. This knowledge is important for modeling the acidification process and either finding ways to counter it or to predict its consequences. The primary purpose of this study was to provide information about how the dynamics of K, Na, Ca, Mg and P are affected by acid deposition in a typical forest in southern China. Materials and Methods: Experimental soils and saplings were collected directly from the monsoon evergreen broad-leaved forest in Dinghushan. All saplings were transplanted individually into ceramic pots in August 2000 and placed in an open area near their origin site. Pot soils were treated weekly from October, 2000 to July, 2002 with an acidic solution at pH 3.05, pH 3.52, pH 4.00 or pH 4.40, or with tap water as a control. The concentrations of SO42-, NO3-, K+, Na+, Ca2+, Mg2+ and available P and the pH were measured in soil and leachate samples taken at different times. The sapling leaves were collected and their element concentrations were measured at the end of the experiment. Results: Concentrations of soil exchangeable Ca and Mg decreased quickly over time, although only Ca showed changes with the acidic solution treatment and soil exchangeable K was stable because of soil weathering. Leaching of K, Mg and Ca was dependent upon the treatment acidity. Soil available P decreased slowly without any correlation with the acidity of the treatment. All the NO3- added by the treatment was taken up by the plants, but the SO42- added accumulated in the soil. Discussion: Amongst the plant species, Schima superba was little affected by the treatment, the leaf P content was affected in Acmena acuminatissima plants and Cryptocarya concinna was the most susceptible species to soil acidification, with a marked decrease of the leaf K, Ca and Mg concentrations when the treatment acidity increased. Conclusions: Simulated acid deposition affected the dynamics of K, Ca and Mg in the monsoon evergreen broad-leaved forest. The dynamics of Ca in the soil and of K, Mg and Ca in the soil leachates were affected by the acidic solution treatment. If such a soil acidification occurs, Cryptocarya concinna will be amongst the first affected species, but Schima superba will be able to sustain a good growth and mineral nutrition. Recommendations and Perspectives: Acid deposition will lead to imbalance the nutrient elements in the evergreen broad-leaved forest because of accelerated leaching losses of soil exchangeable Ca and Mg. Measures should be developed to slow down soil acidification or nutrient decrease.     

Chemical and morphological characteristics of key tree species of the Carpathian Mountains

Concentrations of Al, B, Ca, Cu, Fe, K, Mg, Mn, N, Na, P, S and Zn in the foliage of white fir (Abies alba), Norway spruce (Picea abies) and common beech (Fagus sylvatica) from 25 sites of the Carpathian Mts. forests (Czech Republic, Poland, Romania, Slovakia and Ukraine) are discussed in a context of their limit values. S/N ratio was different from optimum in 90% of localities when compared with the European limit values. Likewise we found increase of Fe and Cu concentrations compared with their background levels in 100% of locations. Mn concentrations were increased in 76% of localities. Mn mobilization values indicate the disturbance of physiological balance leading to the change of the ratio with Fe. SEM-investigation of foliage waxes from 25 sites in the Carpathian Mts. showed, that there is a statistically significant difference in mean wax quality. Epistomatal waxes were damaged as indicated by increased development of net and amorphous waxes. The most damaged stomata in spruce needles were from Yablunitsa, Synevir and Brenna; in fir needles from Stoliky, and in beech leaves from Malá Fatra, Morské Oko and Beregomet. Spruce needles in the Carpathian Mts. had more damaged stomata than fir needles and beech leaves. Spruce seems to be the most sensitive tree species to environmental stresses including air pollution in forests of the Carpathian Mountains. Foliage surfaces of three forest tree species contained Al, Si, Ca, Fe, Mg, K, Cl, Mn, Na, Ni and Ti in all studied localities. Presence of nutrition elements (Ca, Fe, Mg, K and Mn) on foliage surface hinders opening and closing stomata and it is not physiologically usable for tree species.     

Understorey vegetation along a heavy-metal pollution gradient in SW Finland

Understorey vegetation of Scots pine forests was studied along a 8-km transect running SE from a Cu-Ni smelter at Harjavalta, SW Finland. Long-term accumulation of heavy metals and sulphur in the forest ecosystem has drastically changed plant communities. Vegetation was almost absent up to a distance of 0.5 km from the smelter. The total coverage and the number of plant species increased with increasing distance from the smelter. Ordination by global non-metric multidimensional scaling (GNMDS) indicated that the floristic composition was differentiated in response to the pollution level. The main compositional gradient of GNMDS was correlated with the heavy metal concentrations in the organic soil layer and with the size of the overstorey trees. Vascular plants were more pollution-resistant than ground lichens, whereas mosses were the most sensitive plant group. In addition to heavy metals, nutrient imbalances and the considerably reduced water-holding capacity of the surface soil also restrict plant recolonisation on the degraded sites.     

Element concentrations in the forest moss Hylocomium splendens: variation associated with altitude, net primary production and soil chemistry.

Net primary production (NPP) of the forest moss Hylocomium splendens increased significantly along an elevational gradient in the southern Alps of Italy. Extracellularly bound metals (Al, Ca, Co, Cr, Fe, Ni, Mo, Ni, Pb) showed declining concentrations in moss tissue with increasing altitude, presumably because the amount of exchange sites on the cell wall increases less than total biomass. Concentrations of intracellular elements did not vary (Cd, Cu, Mg, Na, Zn), or even increased (K) with altitude. The observed patterns were always independent of precipitation amount and soil concentrations of exchangeable elements. A higher soil nutrient status only enhanced K uptake by the moss. We concluded that variations in moss NPP, associated with elevational gradients, may significantly affect estimates of atmospheric deposition based on moss analysis in mountainous regions.     

Sources of variation in concentrations of nickel and copper in mountain birch foliage near a nickel-copper smelter at Monchegorsk, north-western Russia: results of long-term monitoring

Concentrations of nickel and copper, two principal metal pollutants of the 'Severonikel' smelter at Monchegorsk, NW Russia, were measured in unwashed leaves of mountain birch, Betula pubescens subsp. czerepanovii, collected in eight study sites along the pollution gradient during 1991-2003. In spite of significant decline in metal emissions, concentrations of foliar metals in most of the study sites did not decrease, indicating that soil contamination remains extremely high. Multiyear mean values peaked at 6.6 km S of the smelter, where they were 20-25 times higher than in the most distant study site. Concentrations of both metals demonstrated pronounced annual variation, which was explained by the meteorological conditions of early summer: higher precipitation in May increased foliar concentrations of both metals, whereas higher precipitation in June resulted in lower foliar concentrations of nickel. These data suggest that ecotoxicological situation in metal-contaminated areas can be modified by the expected climate change. In heavily polluted sites individual birch trees generally retained their ranks in terms of metal contamination during 1995-2003, demonstrating that the use of the same set of trees can significantly increase the accuracy of the monitoring data.     

Degradability of ethylenediaminedisuccinic acid (EDDS) in metal contaminated soils: implications for its use soil remediation

Previous research has identified ethylenediaminedisuccinate (EDDS) as a promising biodegradable alternative for persistent compounds such as EDTA for application in soil washing or enhanced phytoextraction of heavy metals. This study examines heavy metal mobilization in three polluted soils varying in soil composition, with specific attention for competitive behaviour for complexation between the various metals and major elements, such as Al, Fe, Mn, Ca and Mg. In addition, amendment biodegradability was compared between the different soil types. The selected soils included a moderately contaminated calcareous clayey soil, a dredged sediment derived surface soil with similar soil characteristics yet more heavily polluted with Cd, Cr and Zn, and a sandy soil moderately contaminated by historical smelter activity (atmospheric deposition). Biodegradability of EDDS in the three soils varied distinctly. This was mainly expressed in the duration of the lag phase prior to metal complex degradation, and not so much in the half life when degradation effectively did set in. Differences in the lag phase were attributed to differences in soil pollution. However, EDDS was fully degraded within a period of 54 d in all soils regardless of initial delay. Assessment of the cation mobilisation patterns in the three soils under study revealed that mainly Ca, Fe and Al can reduce effectiveness of heavy metal mobilisation by competition for complexation.     

Bioindication of air pollution effects near a copper smelter in Brazil using mango trees and soil microbiological properties

A field study near the copper smelter of a large industrial complex examined air pollution effects on vegetation and soil parameters in Cama?ari (northeast Brazil). Close to the smelter, soil pH-value was lower and total acidity as well as organic carbon contents were higher compared with a site far from the source and two reference sites. The acidification of top soil particularly and the drastically enhanced plant-available copper concentrations were caused by atmospheric deposition. High sulphur and copper deposition significantly reduced microbial biomass and altered functional diversity of soil microorganisms (arylsulphatase and xylanase). Large accumulations of sulphur, arsenic and copper were detected in mango leaves (Mangifera indica) growing downwind from the smelter suggesting potential food chain-mediated risk.     

Wetfall Deposition and Precipitation Chemistry for a Central Appalachian Forest

Abstract

Although extensive research on acidic deposition has been directed toward spruce-fir forests, less research has been done on the impacts of air pollution on eastern montane hardwood forests. The purpose of this study was to describe precipitation chemistry for several Appalachian hardwood forest sites at or near the Fernow Experimental Forest (FEF) to assess the potential for problems associated with acidic deposition. Emphasis was placed on seasonal patterns of ionic concentrations (H+, Ca++, NH4+; NO3-, and SO4=) and spatial variability of ionic concentrations and deposition among sites. Seasonal patterns of most ions showed highest concentrations during the summer months and deposition of H+ was especially pronounced during this time. Deposition of all ions was generally greater (related to greater precipitation) at three montane forested sample sites compared to a nonforested riverbottom site. Precipitation chemistry at FEF was similar to other sites throughout the eastern United States and contrasted sharply with mid-western and western sites. Eastern sites, including means for FEF sites, were uniformly 3-4 times higher in H+ and SO4= concentration than the mid-western and western sites. Precipitation at FEF was chronically acidic, more so during the growing season, and highest at higher elevations where environmental stresses can be most severe. Furthermore, there were occasional large discrepancies between the low-elevation site and the higher-elevation forested sites for precipitation chemistry and acidic deposition. These results suggest that synoptic-scale (network) data may greatly underestimate the pollutant conditions to which highelevation forest trees are exposed, since network data rarely take elevation into account and typically are based on annual ionic concentration and deposition means that may be considerably lower than those of the growing season.     

Direct damage to vegetation caused by acid rain and polluted cloud: definition of critical levels for forest trees

The concept of critical levels was developed in order to define short-term and long-term average concentrations of gaseous pollutants above which plants may be damaged. Although the usual way in which pollutants in precipitation (wet deposition) influence vegetation is by affecting soil processes, plant foliage exposed to fog and cloud, which often contain much greater concentrations of pollutant ions than rain, may be damaged directly. The idea of a critical level has been extended to define concentrations of pollutants in wet deposition above which direct damage to plants is likely. Concentrations of acidity and sulphate measured in mountain and coastal cloud are summarised. Vegetation at risk of injury is identified as montane forest growing close to the cloud base, where ion concentrations are highest. The direct effects of acidic precipitation on trees are reviewed, based on experimental exposure of plants to simulated acidic rain, fog or mist. Although most experiments have reported results in terms of pH (H(+) concentration), the accompanying anion is important, with sulphate being more damaging than nitrate. Both conifers and broadleaved tree seedlings showing subtle changes in the structural characteristics of leaf surfaces after exposure to mist or rain at or about pH 3.5, or sulphate concentration of 150 micromol litre(-1). Visible lesions on leaf surfaces occur at around pH 3 (500 micromol litre(-1) sulphate), broadleaved species tending to be more sensitive than conifers. Effects on photosynthesis and water relations, and interactions with other stresses (e.g. frost), have usually been observed only for treatments which have also caused visible injury to the leaf surface. Few experiments on the direct effects of polluted cloud have been conducted under field conditions with mature trees, which unlike seedlings in controlled conditions, may suffer a growth reduction in the absence of visible injury. Although leaching of cations (Ca(2+), Mg(2+), K(+)) is stimulated by acidic precipitation, amounts leached are small compared with root uptake, unless soils have been impoverished. This aspect of the potential effects of acidic precipitation is best considered in terms of the long-term critical-load of pollutants to the soil. Given the practical difficulties in monitoring cloud water composition, a method for defining critical levels is proposed, which uses climatological average data to identify the duration and frequency of hill cloud, and combines this information with measured or modelled concentrations of particulate sulphate in the atmosphere, to derive cloud water concentrations as a function of cloud liquid water content. For forests within 100 m of the cloud base the critical levels of particulate sulphate, corresponding to solution concentrations in the range 150-500 micromol litre(-1), are in the range 1-3.3 microg S m(-3). These concentrations are observed over much of central Europe, suggesting that many montane forests are at risk of direct effects of fossil-fuel-derived pollutants in cloud.     

应用地质累积指数评价抚顺市PM10中元素的污染

2006-2007年采暖季、风沙季和非采暖季分别在抚顺市的6个采样点采集PM10样品,用等离子体原子发射光谱(ICP-AES)法测定样品中Ti、Al、Mn、Mg、Ca、Na、K、Cu、Zn、As、Pb、Cr、Ni、Co、Cd、Fe、V等17种元素的含量,并用地质累积指数对其污染状况进行初步评价。结果表明:(1)从PM10中元素在不同采样点的含量看,抚顺市PM10中Ti、Mn、Mg、Cu、Zn、Pb、Cr、Ni、Co这9种元素在各采样点间的差别较大;Al、Ca、Na、K、As、Cd、Fe、V这8种元素差别较小。(2)从PM10中元素在不同采样季的含量看,抚顺市PM10中Mn、Mg含量的季间差别较大,其余15种元素季间差别较小。(3)Zn、Cd污染较重;Ti、Al、Mg、Ca、Na、K、As、Fe和V污染较轻;其他6种元素在6个采样点和3个采样季污染程度差别较大。(4)水库采样点各元素污染级别均不是最高;新华采样点PM10中Cu、Zn、Pb、Cr、Ni、Co、Cd污染级别均较高。(5)3个采样季PM10中Cd、Zn污染均较重,属于重度或严重污染;在采暖季PM10中Cu、Pb、Cr的地质累积指数较风沙季、非采暖季大;在非采暖季PM10中Mn、Co受到的污染比采暖季和风沙季稍严重。     

Composition of wet and bulk deposition in Erzurum, Turkey

Seasonal variations in the chemical characteristics of wet and bulk deposition samples collected in Erzurum were investigated for the period March 2002-January 2003. Major cations (Ca2+, K+, Mg2+) and major anions (SO4(2-),NO3-) were determined in bulk and wet deposition samples; pH was also measured in wet deposition. The average pH of the wet deposition at Erzurum was 6.6 due to extensive neutralization of the acidity. A strong relationship between pH and SO4(2-) concentrations was observed in all seasons; however, only a weak relationship was found between pH and NO3-. On a seasonal basis, the correlation between Ca2+ and SO4(2-) concentrations was stronger in winter than in summer. Seasonal variations of ions were examined in both wet and bulk deposition samples. Although maximum concentrations of anions generally occurred during winter and spring, cation concentrations peaked in summer for both wet and bulk deposition. Results indicated that Ca2+ was the dominant cation and SO4(2-) the dominant anion in all deposition samples at Erzurum. Even though correlations among the crustal ions (calcium, magnesium and potassium) were high, the relationship between anthropogenic ions (sulfate and nitrate) was less clear in bulk deposition.     

A multivariate statistical analysis of the composition of rainwater near Cubatão, SP, Brazil

With the aim of estimating the chemical composition of bulk precipitation (wet + dry) in Cubat?o, rainwater samples were collected at several localities in the Cubat?o region from May 1984 to October 1985. The levels of some inorganic ions (Na+, Ca2+, K+, Mg2+, Cl-, SO4(2-), NH4+, PO4(3-)) were measured by atomic absorption spectrophotometry, turbidimetry and titration analysis. Correlation and Fisher discrimination indices, principal component and varimax loading and scores were determined in the multivariate statistical data treatment. The results showed that ionic concentrations in rainwater from Vila Parisi are significantly higher than those of Cubat?o Centro, Santos and Serra do Mar. The ionic compositions of the Vila Parisi rainwater seem to be mainly determined by local anthropogenic activities (industrial pollution). Besides the influence of this factor on water quality, there is also a natural oceanic contribution involving Cl- and Na+ at the other locations.     

The combined effects of urea application and simulated acid rain on soil acidification and microbial community structure

Our aim was to test the effects of simulated acid rain (SAR) at different pHs, when applied to fertilized and unfertilized soils, on the leaching of soil cations (K, Ca, Mg, Na) and Al. Their effects on soil pH, exchangeable H⁺ and Al³⁺ and microbial community structure were also determined. A Paleudalfs soil was incubated for 30 days, with and without an initial application of urea (200 mg N kg^?1soil) as nitrogen (N) fertilizer. The soil was held in columns and leached with SAR at three pH levels. Six treatments were tested: SAR of pH 2.5, 4.0 and 5.6 leaching on unfertilized soil (T1, T2 and T3), and on soils fertilized with urea (T4, T5 and T6). Increasing acid inputs proportionally increased cation leaching in both unfertilized and fertilized soils. Urea application increased the initial Ca and Mg leaching, but had no effect on the total concentrations of Ca, Mg and K leached. There was no significant difference for the amount of Na leached between the different treatments. The SAR pH and urea application had significant effects on soil pH, exchangeable H⁺ and Al³⁺. Urea application, SAR treated with various pH, and the interactions between them all had significant impacts on total phospholipid fatty acids (PLFAs). The highest concentration of total PLFAs occurred in fertilized soils with SAR pH5.6 and the lowest in soils leached with the lowest SAR pH. Soils pretreated with urea then leached with SARs of pH 4.0 and 5.6 had larger total PLFA concentrations than soil without urea. Bacterial, fungal, actinomycete, Gram-negative and Gram-positive bacterial PLFAs had generally similar trends to total PLFAs.