Estimate of sea loading by pollutants originating from the littoral counties in the Republic of Croatia

The paper reviews the data of 10 yr of following up sewage (waste water) inflows of anthropological origin into the sea from all the coastal settlements in Croatia. In particular, we report the sea loading by biochemical oxygen demand (BOD₅), expressed by the inhabitant equivalent (I.E.), for seven littoral counties. We estimated the loading of a coastal sea area for each county separately. The objective was to avoid any over- or underestimation of amounts and significance of sewage inflows into the seawater. For this purpose, a precisely determined sea volume up to the 10 m isobath has been chosen. Because of the inadequate treatment of wastewater, the total loading of that maritime territory expressed in terms of i.e. was found to be 26% higher than expected from the actual number of inhabitants. However, daily input represents only 12‰ of the considered volume of the sea. Recipient spent only 0.7% of the disposable oxygen quantity in the sea.     

Solar-induced generation of singlet oxygen and hydroxyl radical in sewage wastewaters

Singlet oxygen (¹O₂) and hydroxyl radical (·OH) play an important role in the degradation of pollutants in surface waters. However, the mechanism underlying the photochemical generation of ¹O₂ and ·OH in wastewaters is poorly known. Here we studied the photo-induced generation of ¹O₂ and ·OH in different sewage treatment plant units. The correlation between the generation of ¹O₂ and ·OH and the water constituents was discussed. Our results show that in sewage units the ¹O₂ formation rate ranges from 2.19 × 10^?8 to 6.74 × 10^?8 mol L^?1 s^?1, and the ·OH formation rate ranges from 1.7 × 10^?11 to 3.06 × 10^?10 mol L^?1 s^?1. The average ¹O₂ formation rates in the various sewage units are similar to those in wetland and estuarine waters containing rich dissolved organic matter and 2–4 times higher than those in lake and seawater samples. The average ·OH formation rates of the sewage units are 5–50 times higher than for other water samples reported. The ·OH generation rate increased with the iron content with a correlation coefficient of 0.85, which indicates that the photo-Fenton reaction plays a dominant role in ·OH generation in sewage wastewater.     

Awareness of sea-level response under climate change on the coast of Ghana

In response to climate change, coastal communities are expected to experience increasing coastal impacts of sea-level rise (SLR). Strategies formulated and implemented to curb these impacts can thus be more effective if scientific findings on the response to climate change and SLR impacts on coastal communities are taken into consideration and not based merely on the need for coastal protection due to physical coastal erosion. There is also the need to determine the level of awareness of sea-level rise and responses in coastal communities to improve adaptation planning. This study assesses the impact of future erosion on the coastal land cover of Ghana. This assessment estimates approximately 2.66 km², 2.77 km², and 3.24 km² of coastal settlements, 2.10 km², 2.20 km² and 2.58 km² of lagoons, 1.39 km², 1.46 km² and 1.71 km² of wetlands to be at risk of inundation by the year 2050 based on coastal erosion estimates for the 2.6, 4.5 and 8.5 Representative Concentration Pathways (RCPs) used in the Fifth Assessment Report (AR5) of the Intergovernmental Panel on Climate Change (IPCC). This study also assesses the level of awareness of respondents to SLR on the coast of Ghana and explores the availability and level of integration of scientific knowledge of SLR into coastal adaptation strategies in Ghana. Assessment of the awareness of SLR responses to the changing climate in Ghana is made through semi-structured interviews at national, municipal/district and coastal community scales. Although settlements may be inundated based on the coastal erosion estimates, coastal dwellers interviewed cherish their proximity to the sea and are determined to maintain their occupancy close to the sea as spatial location influences their source of livelihood (fishing). Respondents lack knowledge/understanding of SLR, as the majority of household interviewees attributed the rise or fall in sea level to God. Respondents from Ngiresia alleged that the ongoing coastal sea defence project in their community has led to increased malaria cases.     

Future ocean acidification will be amplified by hypoxia in coastal habitats

Ocean acidification is elicited by anthropogenic carbon dioxide emissions and resulting oceanic uptake of excess CO₂ and might constitute an abiotic stressor powerful enough to alter marine ecosystem structures. For surface waters in gas-exchange equilibrium with the atmosphere, models suggest increases in CO₂ partial pressure (pCO₂) from current values of ca. 390 μatm to ca. 700–1,000 μatm by the end of the century. However, in typically unequilibrated coastal hypoxic regions, much higher pCO₂ values can be expected, as heterotrophic degradation of organic material is necessarily related to the production of CO₂ (i.e., dissolved inorganic carbon). Here, we provide data and estimates that, even under current conditions, maximum pCO₂ values of 1,700–3,200 μatm can easily be reached when all oxygen is consumed at salinities between 35 and 20, respectively. Due to the nonlinear nature of the carbonate system, the approximate doubling of seawater pCO₂ in surface waters due to ocean acidification will most strongly affect coastal hypoxic zones as pCO₂ during hypoxia will increase proportionally: we calculate maximum pCO₂ values of ca. 4,500 μatm at a salinity of 20 (T = 10 °C) and ca. 3,400 μatm at a salinity of 35 (T = 10 °C) when all oxygen is consumed. Upwelling processes can bring these CO₂-enriched waters in contact with shallow water ecosystems and may then affect species performance there as well. We conclude that (1) combined stressor experiments (pCO₂ and pO₂) are largely missing at the moment and that (2) coastal ocean acidification experimental designs need to be closely adjusted to carbonate system variability within the specific habitat. In general, the worldwide spread of coastal hypoxic zones also simultaneously is a spread of CO₂-enriched zones. The magnitude of expected changes in pCO₂ in these regions indicates that coastal systems may be more endangered by future global climate change than previously thought.     

Hitchhikers reveal cryptic host behavior: new insights from the association between Planes major and sea turtles in the Pacific Ocean

Studies that incorporate information from habitat-specific ecological interactions (e.g., epibiotic associations) can reveal valuable insights into the cryptic habitat-use patterns and behavior of marine vertebrates. Sea turtles, like other large, highly mobile marine vertebrates, are inherently difficult to study, and such information can inform the implementation of conservation measures. The presence of epipelagic epibionts, such as the flotsam crab Planes major, on sea turtles strongly suggests that neritic turtles have recently occupied epipelagic habitats (upper 200 m in areas with >200 m depth) and that epipelagic turtles spend time at or near the surface. We quantified the effects of turtle species, turtle size, and habitat (neritic or epipelagic) on the frequency of epibiosis (F ₀) by P. major on sea turtles in the Pacific Ocean. In neritic habitats, we found that loggerhead (F ₀ = 27.6 %) and olive ridley turtles (F ₀ = 26.2 %) host crabs frequently across a wide range of body sizes, and green turtles almost never host crabs (F ₀ = 0.7 %). These results suggest that loggerheads and olive ridleys display variable/flexible epipelagic-neritic transitions, while green turtles tend to transition unidirectionally at small body sizes. In epipelagic habitats, we found that loggerheads host crabs (F ₀ = 92.9 %) more frequently than olive ridleys (F ₀ = 50 %) and green turtles (F ₀ = 38.5 %). These results suggest that epipelagic loggerheads tend to spend more time at or near the surface than epipelagic olive ridleys and green turtles. Results of this study reveal new insights into habitat-use patterns and behavior of sea turtles and display how epibiont data can supplement data from more advanced technologies to gain a better understanding of the ecology of marine vertebrates during cryptic life stages.     

Suitability of the coastal waters of Sri Lanka for offshore sand mining: a case study on environmental considerations

In Sri Lanka, the total demand for sand is about 12,000,000 m³ per year with a demand growth projected to increase by 10 % every year. However, Sri Lanka’s construction industry seems to face a shortage of sand if offshore sand mining is not promoted as a viable alternative and over-exploitation of river sand may lead to more significant damage to rivers (which is presently a serious issue). This article discusses the suitability or otherwise of the unexplored south-eastern, east and north-western offshore areas for exploration and mining works. This study was conducted by consulting several government organizations and universities dealing with coastal resources management, literature reviews and Key Informants’ Interviews held with Fisher Folk Societies and Divers’ Organizations in the study areas. The east and north-western offshore locations are not ideal considering the bathymetry (most locations in the east coast have water depths >20 m, hence mining is not commercially viable; in the north-western offshore areas depth is <15 m; mining is prohibited in Sri Lanka at depths ≤15 m and <2 km offshore) and the occurrence of critical habitats. In the south-eastern offshore areas the complex wave climate resulting in significant coastal/shoreline stability variations is a concern and the sea is very deep (>20 m beyond 2 km offshore). Therefore, by considering the views expressed by the Divers’ Organizations and Fisher Folk societies it would be ideal to undertake exploration studies in the offshore areas in the north-eastern stretch.     

Exploring the possibilities of seaward migrating juvenile European sturgeon Acipenser sturio L., in the Dutch part of the River Rhine

The water quality of the River Rhine has improved and might again suit the critically endangered European sturgeon A. sturio L, which was extirpated from the river by 1950. This study describes the tracking of 43 juvenile hatchery-reared A. sturio, in the Dutch part of the Lower Rhine and Delta, originating from an ex situ measure of the River Gironde population. Observing in situ juvenile downstream migration could help to identify essential habitats and potential threats, before actual stocking. Fish were implanted with transponders of the NEDAP Trail® system and released in two batches, in May (n?=?13) and June 2012 (n?=?30). Detections collected (n?=?26) exhibited no upstream movement. Test-fish moved downstream with the flow. Because the historic estuary is disconnected from the North Sea by a sea lock “Haringvlietdam”, the migration of the fish followed the re-directed river discharge into the Port of Rotterdam (161 km). 96 % (n?=?19) of the detections was collected from the harbor in brackish water, where fish presumably acclimatized to higher salinities. 14 % (n?=?6) of the sturgeons were recaptured in coastal waters by beam trawlers, five within 1 month after release. It is concluded that sustainable coastal fisheries is a key-condition for rehabilitation of the European sturgeon. Adapted management of the sea lock will reconnect the estuary to the North Sea and create more favorable conditions for the species in the Lower Rhine and Delta.     

Evidence of massive river pollution in the tropical megacity Jakarta as indicated by faecal steroid occurrence and the seasonal flushing out into the coastal ecosystem

In the rapidly growing coastal megacities of developing and emerging countries, the implementation of new sewage treatment facilities is often not keeping pace with the enormous population growth, leading to a deterioration of the urban water resources. Only very few studies discuss faecal water contamination in these urban areas. Our study area Jakarta, the capital of Indonesia, is such a megacity. We investigated chemical markers of faecal contamination in water and sediments from the rivers and canals flowing through Jakarta. Moreover, also the spatial distribution of faecal markers in Jakarta Bay, the coastal ecosystem that receives all urban river discharges, was assessed. The concentrations of the faecal steroid coprostanol in river water ranged from 0.45 to 24.2 µg L^?1, and in sediments from 0.3 to 400 µg g^?1, reflecting the problem of inadequate sewage treatment capacities in the city. The steroid distribution in Jakarta Bay in May 2013 as compared to dry season data indicates a flushing out of particle-associated pollutants from the urban rivers far offshore during the precedent rainy season, where the city experienced a severe flood. This flushing out of particle-associated pollutants during times of heavy rainfall as observed in our study is a pollutant transport mechanism that is important for all tropical coastal systems. The associated impacts on sensitive coastal habitats might become more severe in the future, as a consequence of the expected climate change effects on monsoon variability.     

The potential of marine live-bait introductions into oceanic islands

The likely introduction of non-indigenous species into the coastal ecosystems of oceanic islands through live-bait utilizations was studied. A store survey identified the species that are traded as live fishing-bait. Only Polychaeta species were identified as imported live-bait on São Miguel Island and all specimens were identified as Perinereis linea. Reponses to surveys of 77 anglers showed that: 44 % harvest from native sources, 44 % buy live-bait and 12 % do both; 56 % of the anglers’ fish on a weekly basis; 31 % dispose of the bait into the sea. A field survey at five sites where fishing rods are routinely utilized was developed to investigate if the non-indigenous polychaetes sold as fishing-bait are already established in the natural environment of the Azores archipelago. Three species of Nereididae were identified: the native Neanthes nubila and P. oliveirae and the cryptogenic P. cultrifera. The escalating utilization of live-bait argues for a change in the habits of fishermen with respect to the discarding of leftover bait, as the higher the volume of discards the higher becomes the probability of non-indigenous species establishment successes, especially on oceanic islands characterized by vulnerable ecosystems with simpler trophic webs.     

Soil particle-size composition and coastal erosion and accretion study in Soc Trang mangrove forests

Coastal erosion results in loss of land, which impacts the economy, coastal residents and settlement plans, especially in the context of rising sea levels caused by climate change. Studying soil particle-size fractions in mangrove forests will help provide a better understanding of the relationship between soil proportions and coastal processes as well as the role of mangrove forests to support coastal planning and management. Soil samples were collected at 26 sampling plots at depths of 10 cm and 40 cm in the Cu Lao Dung (CLD), Long Phu (LP) and Vinh Chau (VC) mangrove forests in Viet Nam’s Soc Trang Province. The soil proportions based on six different particle-sizes (<0.074 mm, 0.074–0.1 mm, 0.1–0.25 mm, 0.25–0.5 mm, 0.5–1.0 mm, and >1.0 mm) were measured using a dry sieving method. Analysis showed that soil particle-sizes ranging between 0.074 and 0.5 mm made up 75–95 % of the soil sample weight at both depths. The high standard deviation values of soil proportions of each given particle-size among sampling plots indicated the soil proportions by particle-size varied widely across the sampling plots. Cluster analysis found similar pattern of soil particle-size proportions for samples collected in CLD and VC, and different pattern of soil particle-size proportions in samples collected at LP, which is more impacted by the Mekong River flow and has a thin mangrove forest belt.?Non-metric dimension scaling (NMDS) analysis showed that sampling plots across the landward sites of the three mangrove forest areas were distributed in the nearby locations (Stress?=?0.11). This indicated that soil proportions of particle-sizes of samples collected from areas of the natural forest composed of different species were more similar. Such similarities were not found, however, in samples collected from middle and seaward plots dominated by single-species plantations.     

A very unusual ‘groin’, the MV River Princess,Goa, India and its impacts upon tourism

On June 6, 2000, the Merchant Vessel River Princess – dead weight 114,645 tonnes and 261 m in length, grounded on the beach off the Candolim-Sinquerim coast, Goa, India, spilling some 40 tonnes of oil. In the past 10 years it has settled some 10 m into the sea bed and taken in circa 40,000 tonnes of sand. Until the ‘60’s the Goan economy centered on agriculture and fishing with very low key recreation. Over the last three decades it has become heavily reliant upon recreation, with 13 % of GDP arising from this sector. Nine million cubic metres of littoral drift occurs off this beach per annum, with the longshore current broken by rips into a series of circulating cells. The vessel acts as a massive groin so much so that downdrift, erosion of approximately 5 to 10 m/annum (dependent on the monsoon strength) is occurring at the Taj hotel site. Satellite imagery has shown that some 0.13 km² beach loss has occurred since 2001. Downdrift, the beach is circa 20 m wide compared to updrift areas where it exceeds 100 m. Embryo tombola growth has now commenced between shore and ship. In addition to this, the dune system is now seriously out of equilibrium and sand bags, geotextiles etc. are currently in use to combat dune foot erosion. Questionnaires (n?=?111) showed that 37 % of beach users ranked erosion as the top coastal problem. Sixty seven percent of beach users rated scenery as the most important quality of a beach and due to the grounded ship, >60 % rated the scenery as below average, as well as viewing the ship as a source of danger for swimming purposes.     

Impact of ocean acidification in the metabolism and swimming behavior of the dolphinfish (Coryphaena hippurus) early larvae

Since the industrial revolution, [CO₂]_atm has increased from 280 μatm to levels now exceeding 380 μatm and is expected to rise to 730–1,020 μatm by the end of this century. The consequent changes in the ocean’s chemistry (e.g., lower pH and availability of the carbonate ions) are expected to pose particular problems for marine organisms, especially in the more vulnerable early life stages. The aim of this study was to investigate how the future predictions of ocean acidification may compromise the metabolism and swimming capabilities of the recently hatched larvae of the tropical dolphinfish (Coryphaena hippurus). Here, we show that the future environmental hypercapnia (ΔpH 0.5; 0.16 % CO₂, ~1,600 μatm) significantly (p < 0.05) reduced oxygen consumption rate up to 17 %. Moreover, the swimming duration and orientation frequency also decreased with increasing pCO₂ (50 and 62.5 %, respectively). We argue that these hypercapnia-driven metabolic and locomotory challenges may potentially influence recruitment, dispersal success, and the population dynamics of this circumtropical oceanic top predator.     

GIS-based risk assessment for the Nile Delta coastal zone under different sea level rise scenarios case study: Kafr EL Sheikh Governorate, Egypt

Sea level changes are caused by several natural phenomena, including mainly ocean thermal expansion, glacial melt from Greenland and Antarctica. It was estimated, in this respect, that global average sea level rose, during the 20th Century, by at least 10 cm. This trend is expected to continue and most likely accelerated during the 21st Century due to human-induced global warming. Global average sea level is expected to rise, by the year 2100, due to global warming between 0.18 and 0.59 cm. Such a rise in sea-level will significantly impact coastal areas due to the high concentration of natural and socioeconomic activities and assets located along the coast. The northern coastal zone of the Nile Delta is generally low land, and is consequently vulnerable to direct and indirect impacts of sea level rise (SLR) due to climate changes, particularly inundation. Despite the uncertainty associated with developed scenarios for climate change and expected SLR, there is a need, according to precautionary approach, to assess and analyze the impacts of SLR. Such an assessment, on one hand, can assist in formulating effective adaptation options to specific, sometimes localized, impacts of SLR. On the other hand, such an analysis can contribute significantly to the development of integrated approach to deal with the impacts of SLR. The objective of this paper is to assess and spatially analyze the risks of expected sea level rise (SLR), in particular inundation, and its implications up to the year 2100 in Kafr El Sheikh Governorate, Egypt, using GIS techniques. For that purpose, a GIS was developed for the study area and then utilized to identify the spatial extent of those areas that would be vulnerable to inundation by SLR. Moreover, various land uses/land covers susceptible to such inundation were identified. Results indicate that more than 22.59 % and 24.50 % of the total area of Kafr El Sheikh Governorate would be vulnerable to inundation under B1 and A1FI (IPCC most optimistic and pessimistic scenarios), respectively. No significant difference was noticed between the two scenarios in terms of spatial extent of SLR impacts. It was also found that a significant proportion of these areas were found to be currently either undeveloped or wetlands. Moreover, it was found that about 90.13 % of the vulnerable areas are actually less exposed to the risks of SLR due to the existence of a number of man-made features, not intended as protection measures, e.g. International Coastal Highway, that can be used to limit the areas vulnerable to inundations by SLR.     

A combined coastal protection,realignment and wetland restoration scheme in the southern Baltic: planning process,public information and participation

Markgrafenheide-Hütelmoor covers a total area of 1000 ha (about 490 ha are coastal moor) and a coastline of about 6 km. This touristy area belongs to the city of Rostock in Germany. As response to sea level rise and heavy coastal erosion, the small seaside resort Markgrafenheide received a comprehensive storm surge protection until 2006. Subsequently, the adjacent Hütelmoor was flooded with the aim to restore it as a brackish coastal moor. Coastal protection measures at the Baltic Sea coastline were abandoned to enable natural dynamics, a coastal realignment and salt water intrusions. The entire process until full implementation took 14 years and was associated with very problematic public participation and a strong local polarization. Based on a literature and media review, two surveys, and expert interviews we retrospectively document and analyse the planning process with focus on public information, perception and participation. The local population and holidaymakers did not perceive coastal changes and if, did not associate them with climate change. Interviewees remembered single storm surges, but felt save from it and sea level rise was not perceived as a threat. 89% said that they feel insufficiently informed about the combined coastal protection wetland restoration measure, but did not use the offered information possibilities. 81% had their information from newspapers and freely distributed advertisers. It seems that insufficient information was the major reason for the problems with local acceptance and public participation. The media played a dominating role. The decline of traditional newspapers and the growths of free advertisers seemed to have a negative impact on quality of information and favoured a polarization. Additionally, we discuss local specifics like the cultural background (GDR history), traditions, frustration and the relatively old population and their role in public participation. We strongly promote a pro-active and long-term information and public relation strategy.     

Die Bedeutung in Meerwasser gelöster Glucose für die Ernährung von Anemonia sulcata (Coelenterata: Anthozoa)

Anemonia sulcata Pennant absorbs and accumulates tritiated D-glucose dissolved in sea water in natural concentrations (80 μg/l). The rate of decrease of glucose concentration in the sea water can best be described by an exponential function, when the concentration of added glucose exceeds 20 to 25 μg/l. The glucose, absorbed through the ectoderm, is immediately metabolized. This is determinable experimentally by tritiated water which is emitted into the sea water. The rate of uptake estimated experimentally was in the range 3 to 4 μg glucose/g wet weight/h, whilst the rate calculated by V _max (maximum absorption rate) was 20 μg/g wet weight/h. The concentration of free glucose in the tentacle tissue is greater by a factor of 10⁵ than that of the medium in which absorption takes place. Absorption is temperature-dependent; the Q₁₀ between 15° and 25°C exceeds 2; translocation is, therefore, energy-dependent. Absorption was diminished by inhibitors, especially by phlorizin. The actinians increase their energy reserves by absorption of glucose (and other dissolved organic substances). The energetic net profit by taking up glucose comprises up to 50% of the energy amount which is equivalent to the oxygen consumption.     

苏北盐城海岸带陆源氮磷污染负荷估算初探

海岸带陆源氮磷污染输入是导致近海赤潮的主要原因,但目前尚缺乏系统的调查研究。文章采用野外实地调查和文献调研相结合的方法,从农田径流、养殖废水排放、居民生活污水和工业废水排放等几个方面,初步估算了盐城海岸带陆源氮磷污染负荷的分配情况。结果表明,盐城海岸带主要陆源污染源中,养殖水域氮磷排放总量为7641t和480t,分别占排放总量的75.1%、63.5%;其次为居民工矿的生活生产污水,其氮磷排放量为2083t和232t,占总排放量的20.5%、30.7%;农田目前看来不是盐城海岸带主要的陆源污染源,排放量仅占4.4%和5.8%。在当前海岸带陆源污染源治理中,除采取有力措施控制养殖水域养分流失外,农村和乡镇生活生产污水的排放也应引起重视。     

Impact of ocean acidification on escape performance of the king scallop, Pecten maximus, from Norway

The ongoing process of ocean acidification already affects marine life, and according to the concept of oxygen and capacity limitation of thermal tolerance, these effects may be intensified at the borders of the thermal tolerance window. We studied the effects of elevated CO₂ concentrations on clapping performance and energy metabolism of the commercially important scallop Pecten maximus. Individuals were exposed for at least 30 days to 4 °C (winter) or to 10 °C (spring/summer) at either ambient (0.04 kPa, normocapnia) or predicted future PCO₂ levels (0.11 kPa, hypercapnia). Cold-exposed (4 °C) groups revealed thermal stress exacerbated by PCO₂ indicated by a high mortality overall and its increase from 55 % under normocapnia to 90 % under hypercapnia. We therefore excluded the 4 °C groups from further experimentation. Scallops at 10 °C showed impaired clapping performance following hypercapnic exposure. Force production was significantly reduced although the number of claps was unchanged between normocapnia- and hypercapnia-exposed scallops. The difference between maximal and resting metabolic rate (aerobic scope) of the hypercapnic scallops was significantly reduced compared with normocapnic animals, indicating a reduction in net aerobic scope. Our data confirm that ocean acidification narrows the thermal tolerance range of scallops resulting in elevated vulnerability to temperature extremes and impairs the animal’s performance capacity with potentially detrimental consequences for its fitness and survival in the ocean of tomorrow.     

Long-term and trans-life-cycle effects of exposure to ocean acidification in the green sea urchin Strongylocentrotus droebachiensis

Anthropogenic CO₂ emissions are acidifying the world’s oceans. A growing body of evidence demonstrates that ocean acidification can impact survival, growth, development and physiology of marine invertebrates. Here, we tested the impact of long-term (up to 16 months) and trans-life-cycle (adult, embryo/larvae and juvenile) exposure to elevated pCO₂ (1,200 μatm, compared to control 400 μatm) on the green sea urchin Strongylocentrotus droebachiensis. Female fecundity was decreased 4.5-fold when acclimated to elevated pCO₂ for 4 months during reproductive conditioning, while no difference was observed in females acclimated for 16 months. Moreover, adult pre-exposure for 4 months to elevated pCO₂ had a direct negative impact on subsequent larval settlement success. Five to nine times fewer offspring reached the juvenile stage in cultures using gametes collected from adults previously acclimated to high pCO₂ for 4 months. However, no difference in larval survival was observed when adults were pre-exposed for 16 months to elevated pCO₂. pCO₂ had no direct negative impact on juvenile survival except when both larvae and juveniles were raised in elevated pCO₂. These negative effects on settlement success and juvenile survival can be attributed to carry-over effects from adults to larvae and from larvae to juveniles. Our results support the contention that adult sea urchins can acclimate to moderately elevated pCO₂ in a matter of a few months and that carry-over effects can exacerbate the negative impact of ocean acidification on larvae and juveniles.     

Effect of humic acids,nitrate and oxygen on the photodegradation of the flubendiamide insecticide: identification of products

Flubendiamide is a ryanodine insecticide that shows a strong insecticidal activity and is relatively safe for non-target organisms. Actually only flubendiamide and its product desiodo-flubendiamide have been studied during catalytic degradation using TiO₂ and ZnO. Therefore, here we tested the photocatalytic removal of flubendiamide in the presence of nitrates or humic acids. Degradation kinetics were monitored using high-performance liquid chromatography ultraviolet–visible detector. Product identification was done using a high-resolution time-of-flight mass spectrometer coupled to a gas chromatograph (GC-HRMS). Results show that the addition of humic acids at 10 mg l^?1 increased the removal of flubendiamide more than five times. The addition of nitrate ions at 10 mg l^?1 had no influence. The removal of flubendiamide was more than ten times faster in experiments with oxygen purging. Fourteen degradation products were identified, which can be classified into three groups: phthalimide and related phthalic acid derivatives, fluorinated species related to the second amide moiety, and advanced transformation products.     

Hypoxia-driven selective degradation of cellular proteins in jumbo squids during diel migration to the oxygen minimum zones

The jumbo squid, Dosidicus gigas, is an oceanic top predator in the eastern tropical Pacific that undergoes diel vertical migrations into mesopelagic oxygen minimum zones (OMZs). Besides glycogen breakdown, the pathways of the squid’s metabolic (suppression) strategy are poorly understood. Here, juvenile D. gigas were exposed to oxygen levels found in the OMZ off Gulf of California (1 % O₂, 1 kPa at 10 °C) with the aim to identify, via proteomic tools, eventual anaerobic protein degradation as potential energy source at such depths. Under hypoxia, total protein concentration decreased nonsignificantly from 79.2 ± 12.4 mg g^?1 wet mass to 74.7 ± 11.7 mg g^?1 wet mass (p > 0.05). Yet, there was a significant decrease in heat-shock protein (Hsp) 90 and α-actinin contents (p < 0.05). The lower α-actinin concentration at late hypoxia was probably related to decreased protection of the Hsp90 chaperon machinery resulting in increased ubiquitination (p < 0.05) and subsequent degradation. Thus, the present findings indicate that D. gigas might degrade, at least under progressing hypoxia, specific mantle proteins anaerobically to increase/maintain anaerobic ATP production and extend hypoxia exposure time. Moreover, the ubiquitin–proteasome system seems to play an important role in hypoxia tolerance, but further investigations are necessary to discover its full potential and pathways.