中药复方对锦鲤诱食活性及促生长的影响

以锦鲤为试验对象,研究中药复方制剂对锦鲤的诱食活性及促生长的影响。实验分为4组,Ⅰ为对照组,饲喂基础饲料;Ⅱ、Ⅲ、Ⅳ为实验组,分别在基础饲料中添加150mL/kg、200mL/kg、250mL/kg的中药复方。喂食20d后的结果表明,各实验组均有较强的诱食活性(P<0.05),Ⅱ组效果最显著(P<0.01);Ⅱ组和Ⅲ组质量增加率和肥满度均显著高于对照组(P<0.05),Ⅱ组效果最显著(P<0.01),各实验组均能明显降低饲料系数(P<0.05)。     

中药复合饲料对锦鲤诱食效果与生长影响的研究

选择3种中药配方,以其水提液对体长3-5cm锦鲤进行诱食活性(触球法)试验.每组实验重复5次,实验结果采用Excel和SPSS软件分析.结果表明,配方1对锦鲤的诱食有抑制作用,配方2与配方3对锦鲤有诱食反应,抑制或诱食的强弱与中药的浓度有关.其中,配方1 P=0.004＜ 0.05,配方2 P=0.0005＜ 0.05,配方3 P=0.0003＜ 0.0,经复合饵料饲喂后,实验鱼体长和体重都比基础饵料增长显著,说明3种饵料对锦鲤的生长都有促进作用.其中,配方2降低饵料系数0.2801,增重率达17.66％.     

4种实用配方饲料对锦鲤诱食活性和生长的影响

试验用鱼选择锦鲤.根据动物摄食行为学原理,采用迷宫法和摄食率法为试验方法,配制4种添加甘氨酸的实用饵料,研究其对锦鲤诱食活性和生长的影响,实验结果用Excel和SPSS17.0软件进行分析.结果表明,4种实用饵料都对锦鲤具有诱食反应.其中,火棘(35m甘氨酸)P=0.003＜ 0.05,浒苔(35mg甘氨酸)P=0.001＜0.05,诱食效果有极显著意义.与对照组相比,喂食实用饵料的锦鲤增重率都有所提高,其中浒苔+50mg甘氨酸最为明显,达到8.44％,饵料系数降低到4.123.     

19种动植物粗提物对锦鲤诱食活性的研究

以16种植物和3种动物提取物作为实验材料,以体长为3—5cm的锦鲤为实验对象,采用触球法进行诱食活性研究,5次重复试验,并利用SPSS软件进行分析。结果表明,大蒜、浒苔、洋葱、西红柿、江蓠、陈皮、橙子、山楂、紫菜提取物和海星、海蜇、牡蛎提取物对锦鲤显示出诱食反应。其中,大蒜P=0.0010.05、浒苔P=0.0020.05、江蓠P=0.0040.05、陈皮P=0.0370.05、海星P=0.0030.05,说明大蒜、浒苔、江蓠、陈皮、海星对锦鲤的诱食反应有显著意义。     

氨基酸强化饵料对锦鲤诱食活性的影响

选择极具观赏性质的杂食性鱼类——锦鲤作为实验研究对象,以12种氨基酸分别配成浓度为0.05mol/L的氨基酸溶液为诱食剂,采用摄食行为学方法(触球法和迷宫法)作为试验方法,记录实验数据,并用Excel和SPSS软件进行分析。结果表明,甘氨酸P=0.0110.05、组氨酸P=0.0080.05、精氨酸P=0.0280.05,说明甘氨酸、组氨酸、精氨酸对锦鲤的引诱效果具有显著的统计意义。     

不同中药复方对锦鲤免疫功能的影响

以锦鲤为实验研究对象,研究3种中药复方制剂对锦鲤免疫功能的影响。结果表明,3种中药复方制剂对锦鲤血清谷丙转氨酶、谷草转氨酶活性和尿素氮含量均有一定的降低诱导作用,1号（黄芪、当归、益母草等）复方组的尿素氮含量降低显著（P〈0.05）;对锦鲤肝脏免疫能力均有增强作用,1号（黄芪、当归、益母草等）、3号（灵芝、大黄、黄柏等）复方组可显著提高肝脏中超氧化物歧化酶（SOD）活性和总抗氧化能力（P〈0.05）,2号（红景天、刺五加、香菇等）复方组可使肝脏溶菌酶活性显著升高（P〈0.05）。     

水产饲料中浒苔添加量与加工工艺研究

研究了浒苔在水产饲料中的添加量和最适加工工艺。以海洋经济鱼类梭鱼为实验对象，做了两个阶段性实验：第一阶段在饲料中按不同比例添加浒苔研究对梭鱼生长的影响。1组为对照组，添加量为0；2—4组为实验组，浒苔添加量分别为10％、15％、20％。保持各组的营养水平基本一致，实验10d，采取摄食率法作为实验方法，记录实验数据，经Excel分析表明，与对照组相比，实验组的各项生长指标高于对照组，其中第3组最优，增重率提高了14．29％，饵料系数降低了0．6。第二阶段研究不同工艺的浒苔饲料对海洋梭鱼生长性能的影响。以新鲜浒苔为基础工艺组，以浒苔干粉、添加柠檬酸沸煮、冷冻处理浒苔为实验组，即工艺一、工艺二、工艺三。同样以摄食率法作为实验方法，实验期10d，记录实验数据，经Excel分析表明，工艺一和工艺二增重率分别提高了6．37％、7．37％，工艺二饵料系数为2．83。     

海星多糖对小鼠酒精性脂肪肝损伤的预防研究

以多棘海盘车体壁为材料提取海星多糖,探讨多糖对小鼠酒精肝损伤的预防作用。采用水提法提取多糖,硫酸—苯酚法进行测定。以正常小鼠作空白对照,将酒精肝小鼠分为酒精肝模型组,海星多糖低、中、高剂量组(20mg/kg、40mg/kg、80mg/kg)进行灌胃,测定小鼠生理指标。结果显示,与模型组相比,海星多糖组小鼠血清中ALT、AST含量降低(P<0.05),TG、MDA含量也降低(P<0.05),GSH-PX、SOD活性升高(P<0.05),肝脏脂肪变性明显减轻,说明海星多糖对酒精性肝脏有较明显的保护作用。     

湛江市主要城市集中式饮用水水源地基础环境状况与保护对策

对湛江市主要城市集中式饮用水水源地基础环境状况的进行的调查,采用单因子法对选取的9个饮用水水源地的水质进行了评价。结果表明,2012～2016年南渡河、鉴江2个水源地年均水质均不达标,水质类别为《地表水环境质量标准》(GB3838-2002)Ⅳ类标准,超标项目均为粪大肠菌群;其余7个水源地监测断面水质良好,均达到Ⅲ类标准,其中雷州青年运河水质最好,为Ⅱ类;各水源地水质总体保持稳定。分析了该饮用水水源地存在的主要环境问题和管理问题,并提出了加强水污染防治、完善管理政策和应急预案等对策及建议。     

土壤锑污染对桑树的影响初探

运用盆栽实验和实验分析方法,研究了土壤锑(Sb)污染对桑叶品质的影响,揭示了桑树对土壤锑的耐性机制。在土壤中分别添加三价锑15mg/kg、40mg/kg、80mg/kg、120mg/kg、160mg/kg和300mg/kg,以植物生长指标及生理指标为测试指标,实验周期60d。实验结果表明,低浓度Sb处理(<40mg/kg)对桑树生长有促进作用;随着土壤Sb浓度增高(40—300mg/kg),它对桑树产生抑制效应。但当土壤锑浓度不超过160mg/kg时,桑树对土壤锑污染有一定的耐性,其耐性指数>0.8。土壤锑污染对桑叶叶绿素含量、淀粉含量和可溶性糖含量影响不显著,桑叶中的锑含量随土壤锑含量的增加而增多。     

Nitrogen excretion and ammonia emissions from pigs fed modified diets

Two swine feeding trials were conducted (initial body weight = 47 +/- 2 and 41 +/- 3 kg for Trials 1 and 2, respectively) to evaluate reduced crude protein (CP) and yucca (Yucca schidigera Roezl ex Ortgies) extract-supplemented diets on NH3 emissions. In Trial 1, nine pigs were offered a corn-soybean meal diet (C, 174 g kg(-1) CP), a Lys-supplemented diet (L, 170 g kg(-1) CP), or a 145 g kg(-1) CP diet supplemented with Lys, Met, Thr, and Trp (LMTT). In Trial 2, nine pigs were fed diet L supplemented with 0, 62.5, or 125 mg of yucca extract per kg diet. Each feeding period consisted of a 4-d dietary adjustment followed by 72 h of continuous NH3 measurement. Urine and fecal samples were collected each period. Feeding the LMTT diet reduced (P < 0.05) average daily gain (ADG) and feed efficiency (G:F) compared to diet L. Fecal N concentration decreased with a reduction in dietary CP, but urinary ammonium increased from pigs fed diet LMTT (2.0 g kg(-1), wet basis) compared to those fed diet C (1.1 g kg(-1)) or L (1.0 g kg(-1)). When pigs were fed reduced CP diets NH3 emission rates decreased (2.46, 2.16, and 1.05 mg min(-1) for diets C, L, and LMTT). Yucca had no effect on feed intake, ADG, or G:F. Ammonium and N concentrations of manure and NH3 emission rates did not differ with yucca content. Caution must be executed to maintain animal performance when strategies are implemented to reduce NH3 emissions.     

Manipulation of dietary protein and nonstarch polysaccharide to control swine manure emissions

Odor and greenhouse gas (GHG) emissions from stored pig (Sus scrofa) manure were monitored for response to changes in the crude protein level (168 or 139 g kg(-1), as-fed basis) and nonstarch polysaccharide (NSP) content [i.e., control, or modified with beet pulp (Beta vulgaris L.), cornstarch, or xylanase] of diets fed to pigs in a production setting. Each diet was fed to one of eight pens of pigs according to a 2 x 4, full-factorial design, replicated over three time blocks with different groups of animals and random assignment of diets. Manure from each treatment was characterized and stored in a separate, ventilated, 200-L vessel. Repeated measurements of odor, carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) emissions from the vessels were taken every two weeks for eight weeks. Manure from high-protein diets had higher sulfur concentration and pH (P < or = 0.05). High-NSP (beet pulp) diets resulted in lower manure nitrogen and ammonia concentrations and pH (P < or = 0.05). Odor level and hedonic tone of exhaust air from the storage vessel headspaces were unaffected by the dietary treatments. Mean CO2 and CH4 emissions (1400 and 42 g d(-1) m(-3) manure, respectively) increased with lower dietary protein (P < or = 0.05). The addition of xylanase to high-protein diets caused a decrease in manure CO2 emissions, but an increase when added to low-protein diets (P < or = 0.05). Nitrous oxide emissions were negligible. Contrary to other studies, these results do not support the use of dietary protein reduction to reduce emissions from stored swine manure.     

Phytase,high-available-phosphorus corn,and storage effects on phosphorus levels in pig excreta

Phosphorus-based land application limits for manure have increased the importance of optimizing diet P management and accurately characterizing the bioavailability of manure P. We examined the effects of pig (Sus scrofa) diets formulated with high-available-P corn and phytase on P levels in excreta and slurry stored for 30, 60, 90, 120, and 150 d. Twenty-four pigs (approximately 14 kg each) were fed one of four low-P diets: (i) normal corn, no phytase (control); (ii) normal corn with 600 phytase units kg(-1) (PHY); (iii) high-available-P corn, no phytase (HAP); and (iv) high-available-P corn with 600 phytase units kg(-1) (HAP + PHY). Fresh fecal and stored slurry dry matter (DM) was analyzed for total phosphorus (TP), dissolved molybdate-reactive phosphorus (DRP), dissolved organic phosphorus (DOP), acid-soluble reactive phosphorus (ASRP), acid-soluble organic phosphorus (ASOP), and phytate phosphorus (PAP). The PHY, HAP, and HAP + PHY diets significantly (alpha = 0.05) decreased fecal TP 19, 17, and 40%, respectively, compared with the control. Dissolved reactive P was 36% lower in the HAP + PHY diet compared with the other diets. Relative fractions (percent of TP) of DRP, DOP, ASOP, and PAP in slurry generally decreased with storage time up to 150 d, with the largest decreases occurring within 60 to 90 d. Diet-induced differences in relative fractions of DRP, DOP, ASRP, and PAP were significant when averaged across storage times, simulating a mixed-age slurry. Relative fractions of DRP in simulated mixed-age slurries were higher in HAP and HAP + PHY diets, indicating that diet may affect P losses under certain P-based application scenarios.     

Using electromagnetic induction technology to predict volatile fatty acid, source area differences

Subsurface measures have been adapted to identify manure accumulation on feedlot surfaces. Understanding where manure accumulates can be useful to develop management practices that mitigate air emissions from manure, such as odor or greenhouse gases. Objectives were to determine if electromagnetic induction could be used to predict differences in volatile fatty acids (VFA) and other volatiles produced in vitro from feedlot surface material following a simulated rain event. Twenty soil samples per pen were collected from eight pens with cattle fed two different diets using a predictive sampling approach. These samples were incubated at room temperature for 3 d to determine fermentation products formed. Fermentation products were categorized into acetate, straight-, branched-chained, and total VFAs. These data were used to develop calibration prediction models on the basis of properties measured by electromagnetic induction (EMI). Diet had no significant effect on mean volatile solids (VS) concentration of accumulated manure. However, manure from cattle fed a corn (Zea mays L.)-based diet had significantly ( P ≤ 0.1) greater mean straight-chained and total VFA generation than pens where wet distillers grain with solubles (WDGS) were fed. Alternately, pens with cattle fed a WDGS-based diet had significantly (P ≤ 0.05) greater branched-chained VFAs than pens with cattle fed a corn-based diet. Many branched-chain VFAs have a lower odor threshold than straight-chained VFAs; therefore, emissions from WDGS-based diet manure would probably have a lower odor threshold. We concluded that diets can affect the types and quantities of VFAs produced following a rain event. Understanding odorant accumulation patterns and the ability to predict generation can be used to develop precision management practices to mitigate odor emissions.     

Phytase supplementation and reduced-phosphorus turkey diets reduce phosphorus loss in runoff following litter application

Concerns about regional surpluses of manure phosphorus (P) leading to increased P losses in runoff have led to interest in diet modification to reduce P concentrations in diets. The objectives of this study were to investigate how dietary P amendment affected P concentrations in litters and P losses in runoff following land application. We grew two flocks of turkeys on the same bed of litter using diets with two levels of non-phytate phosphorus (NPP), with and without phytase. The litters were incorporated into three soils in runoff boxes at a plant-available nitrogen (PAN) rate of 168 kg PAN/ha, with runoff generated on Days 1 and 7 under simulated rainfall and analyzed for dissolved reactive phosphorus (DRP) and total P. Litters were analyzed for water-soluble phosphorus (WSP) and total P, while soils in the runoff boxes were analyzed for WSP and Mehlich-3 phosphorus (M3-P). Formulating diets with lower NPP and phytase both decreased litter total P. Phytase had no significant effect on litter WSP at a 1:200 litter to water extraction ratio, but decreased WSP at a 1:10 extraction ratio. Using a combination of reducing NPP fed and phytase decreased the total P application rate by up to 38% and the P in surplus of crop removal by approximately 48%. Reducing the NPP fed reduced DRP in runoff from litter-amended soils at Day 1, while phytase had no effect on DRP concentrations. Increase in soil M3-P was dependent on total P applied, irrespective of diet. Reducing overfeeding of NPP and utilizing phytase in diets for turkeys should decrease the buildup of P in soils in areas of intensive poultry production, without increasing short-term concerns about dissolved P losses.