Management of microbial contamination in storm runoff from California coastal dairy pastures

A survey of storm runoff fecal coliform bacteria (FCB) from working farm and ranch pastures is presented in conjunction with a survey of FCB in manure management systems (MMS). The cross-sectional survey of pasture runoff was conducted on 34 pastures on five different dairies over 2 yr under varying conditions of precipitation, slope, manure management, and use of conservation practices such as vegetative filter strips. The MMS cross-sectional survey consisted of samples collected during 1 yr on nine different dairies from six loafing barns, nine primary lagoons, 12 secondary lagoons, and six irrigation sample points. Pasture runoff samples were additionally analyzed for Cryptosporidium sp. and Giardia duodenalis, whereby detectable concentrations occurred sporadically at higher FCB concentrations resulting in poor correlations with FCB. Prevalence of both parasites was lower relative to high-use areas studied simultaneously on these same farms. Application of manure to pastures more than 2 wk in advance of storm-associated runoff was related to a > or =80% reduction in FCB concentration and load compared to applications within 2 wk before a runoff event. For every 10 m of buffer length, a 24% reduction in FCB concentration was documented. A one-half (75%), one (90%), and two (99%) log10 reduction in manure FCB concentration was observed for manure holding times in MMS of approximately 20, 66, and 133 d, respectively. These results suggest that there are several management and conservation practices for working farms that may result in reduced FCB fluxes from agricultural operations.     

Modeling watershed-scale effectiveness of agricultural best management practices to reduce phosphorus loading

Planners advocate best management practices (BMPs) to reduce loss of sediment and nutrients in agricultural areas. However, the scientific community lacks tools that use readily available data to investigate the relationships between BMPs and their spatial locations and water quality. In rural, humid regions where runoff is associated with saturation-excess processes from variable source areas (VSAs), BMPs are potentially most effective when they are located in areas that produce the majority of the runoff. Thus, two critical elements necessary to predict the water quality impact of BMPs include correct identification of VSAs and accurate predictions of nutrient reduction due to particular BMPs. The objective of this research was to determine the effectiveness of BMPs using the Variable Source Loading Function (VSLF) model, which captures the spatial and temporal evolutions of VSAs in the landscape. Data from a long-term monitoring campaign on a 164-ha farm in the New York City source watersheds in the Catskills Mountains of New York state were used to evaluate the effectiveness of a range of BMPs. The data spanned an 11-year period over which a suite of BMPs, including a nutrient management plan, riparian buffers, filter strips and fencing, was installed to reduce phosphorus (P) loading. Despite its simplicity, VSLF predicted the spatial distribution of runoff producing areas well. Dissolved P reductions were simulated well by using calibrated reduction factors for various BMPs in the VSLF model. Total P losses decreased only after cattle crossings were installed in the creek. The results demonstrated that BMPs, when sited with respect to VSAs, reduce P loss from agricultural watersheds, providing useful information for targeted water quality management.     

Environmental load of Cryptosporidium parvum oocysts from cattle manure in feedlots from the central and western United States

The first step in assessing the risk of water contamination by Cryptosporidium parvum oocysts from feedlot cattle (Bos taurus) production systems is to quantify the number of C. parvum oocysts present in the fecal material deposited by feedlot cattle. Our primary objective for this project was to estimate the daily environmental load of C. parvum oocysts in fecal material deposited by feedlot cattle from across the central and western USA. Our secondary goal was to genotype isolates of C. parvum from feedlot cattle to help facilitate proper identification of mammalian sources of waterborne C. parvum. Based on 5274 fecal samples from 22 feedlots in seven states (California, Washington, Colorado, Oklahoma, Texas, Nebraska, and South Dakota), we estimated a point prevalence of C. parvum of 0.99 to 1.08% in fecal material from feedlot pens from a wide range of climates and a diverse range of feedlot management systems. On average, fresh fecal material from throughout feedlot systems (recent arrivals to nearing slaughter) contained about 1.3 to 3.6 oocysts/g feces, which roughly translates to about 2.8 x 10(4) to 1.4 x 10(5) oocysts/animal per day.     

STORM WATER RUNOFF QUALITY FROM THREE LAND-USE AREAS IN SOUTH FLORIDA1

ABSTRACT: Storm water runoff studies of three small basins (20, 40, and 58 acres) in the Fort Lauderdale area of Florida were conducted by the U.S. Geological Survey in 1974–78. The basins were homogeneously developed with land uses being: commercial, single family residential, and high traffic volume highway. Synchronized data were collected for rainfall, storm water discharge, storm water quality, and bulk precipitation (rainfall plus dry fallout) quality. Analysis of the storm water discharge data showed that most runoff was from impervious areas hydraulically connected to drain inlets. Regression analyses of the storm water discharge and water quality data indicated that storm loads from the single family residential area correlated strongly with peak discharge and length of antecedent dry periods. Storm loads from the highway area correlated strongly with rainfall and less strongly with peak discharge and antecedent dry periods. Storm loads from the commercial area correlated strongly with peak discharge and rainfall, and less strongly with antecedent dry periods. On a unit area basis, the single family residential area yielded the largest loads of nitrogen, phosphorus, and dissolved solids. The commercial area yielded the largest loads of lead, zinc, and chemical oxygen demand. Yields of carbon were about the same for the three areas. Constituent loadings derived directly from the atmosphere were estimated on the basis of bulk precipitation samples and compared with storm runoff loads from the highway and commercial areas.     

Combined monitoring and modeling indicate the most effective agricultural best management practices

Although water quality problems associated with agricultural nonpoint source (NPS) pollution have prompted the rapid and widespread adoption of a variety of so called "best management practices" (BMPs), there have been few realistic efforts to assess their combined effectiveness in reducing NPS pollution. This study used the Variable Source Loading Function (VSLF) model, a distributed watershed model, to simulate phosphorus (P) loading from an upstate New York dairy farm before and after the implementation of a suite of BMPs. With minimal calibration, the model calculates the dissolved P (DP) losses from impervious surfaces (e.g., barnyards), the plant/soil complex, field-applied manure, and loads associated with baseflow conditions. The simulated DP loads agreed well with measured loads for both the pre-BMP and post-BMP periods. More importantly, results showed that BMPs reduced DP loads by 35%, which is over half of the expected reduction if all manure was removed from the watershed, i.e., approximately 50% reduction. The model results indicate that had no BMPs been installed DP loads would be approximately 37% greater than observed at the watershed outlet. The most effective BMPs were those that disassociated pollutant loading areas from areas prone to generating runoff, i.e., hydrologically sensitive areas. By contrast, attempts to reduce P content in manure were somewhat less effective. This study demonstrates that a combination of distributed, mechanistic modeling and long-term monitoring provides better insights into the effectiveness of water quality protection efforts than either individually.     

Dry Weather Water Quality Loadings in Arid,Urban Watersheds of the Los Angeles Basin,California, USA1

Abstract: Dry weather runoff in arid, urban watersheds may consist entirely of treated wastewater effluent and/or urban nonpoint source runoff, which can be a source of bacteria, nutrients, and metals to receiving waters. Most studies of urban runoff focus on stormwater, and few have evaluated the relative contribution and sources of dry weather pollutant loading for a range of constituents across multiple watersheds. This study assessed dry weather loading of nutrients, metals, and bacteria in six urban watersheds in the Los Angeles region of southern California to estimate relative sources of each constituent class and the proportion of total annual load that can be attributed to dry weather discharge. In each watershed, flow and water quality were sampled from storm drain and treated wastewater inputs, as well as from in‐stream locations during at least two time periods. Data were used to calculate mean concentrations and loads for various sources. Dry weather loads were compared with modeled wet weather loads under a range of annual rainfall volumes to estimate the relative contribution of dry weather load. Mean storm drain flows were comparable between all watersheds, and in all cases, approximately 20% of the flowing storm drains accounted for 80% of the daily volume. Wastewater reclamation plants (WRP) were the main source of nutrients, storm drains accounted for almost all the bacteria, and metals sources varied by constituent. In‐stream concentrations reflected major sources, for example nutrient concentrations were highest downstream of WRP discharges, while in‐stream metals concentrations were highest downstream of the storm drains with high metals loads. Comparison of wet vs. dry weather loading indicates that dry weather loading can be a significant source of metals, ranging from less than 20% during wet years to greater than 50% during dry years.     

Multivariate analysis of paired watershed data to evaluate agricultural best management practice effects on stream water phosphorus

Quantification of the effects of management programs on water quality is critical to agencies responsible for water resource protection. This research documents reductions in stream water phosphorus (P) loads resulting from agricultural best management practices (BMPs) implemented as part of an effort to control eutrophication of Cannonsville Reservoir, a drinking water supply for New York City. Dairy farms in the upstate New York reservoir basin were the target of BMPs designed to reduce P losses. A paired watershed study was established on one of these farms in 1993 to evaluate changes in P loading attributable to implementation of BMPs that included manure management, rotational grazing, and improved infrastructure. Intensive stream water monitoring provided data to calculate P loads from the 160-ha farm watershed for all runoff events during a two-year pre-treatment period and a four-year post-treatment period. Statistical control for inter-annual climatic variability was provided by matched P loads from a nearby 86-ha forested watershed, and by several event flow variables measured at the farm. A sophisticated multivariate analysis of covariance (ANCOVA) provided estimates of both seasonal and overall load reductions. Statistical power and the minimum detectable treatment effect (MDTE) were also calculated. The results demonstrated overall event load reductions of 43% for total dissolved phosphorus (TDP) and 29% for particulate phosphorus (PP). Changes in farm management practices and physical infrastructure clearly produced decreases in event P losses measurable at the small watershed scale.     

STORM AND SEASONAL DISTRIBUTIONS OF FECAL COLIFORMS AND CRYPTOSPORIDIUM IN A SPRING1

ABSTRACT: The transmission of disease in ground water is a topic of great concern to government agencies, ground water specialists, and the general public. The purpose of this study was to compare the temporal variability in storm flow of fecal coliform bacteria densities and Cryptosporidium parvum oocyst densities in agriculturally impacted karst ground water. Cryptosporidium parvum oocyst densities ranged from 0 to 1,050 oocysts/1, and mean storm densities ranged from 3.5 to 156.8 oocysts/1. Fecal coliform densities ranged from less than 1 CFU/100ml to more than 40,000 CFU/100ml, and geometric mean storm densities ranged from 1.7 CFU/100ml to more than 7,000 CFU/100ml. Fecal coliform densities correlated well with flow during storms, but Cryptosporidium oocyst densities exhibited a great deal of sample to sample variability and were not correlated with flow. Fecal coliform densities did not correlate positively with Cryptosporidium oocyst densities. Fecal coliform densities were greatest at storm peaks, when sediment loads were also greatest. Multiple transport mechanisms for fecal coliform bacteria and C. parvum oocysts may necessitate various agricultural land management and livestock health maintenance practices to control movement of pathogens to karst ground water.     

Efficacy of vegetated buffer strips for retaining Cryptosporidium parvum

Overland and shallow subsurface hydrologic transport of pathogenic Cryptosporidium parvum oocysts from cattle feces into surface drinking water supplies is a major concern on annual grasslands in California's central and southern Sierra Nevada foothills. Soil boxes (0.5 m wide x 1.1 m long x 0.3 m deep) were used to evaluate the ability of grass vegetated buffer strips to retain 2 x 10(8) spiked C. parvum oocysts in 200-g fecal deposits during simulated rainfall intensities of 30 to 47.5 mm/h over 2 h. Buffers were comprised of Ahwahnee sandy loam (coarse-loamy, mixed, active, thermic Mollic Haploxeralfs; 78:18:4 sand to silt to clay ratio; dry bulk density = 1.4 g/cm(3)) set at 5 to 20% land slope, and >/=95% grass cover (grass stubble height = 10 cm; biomass = 900 kg/ha dry weight). Total number of oocysts discharged from each soil box (combined overland and subsurface flow) during the 120-min simulation ranged from 1.5 x 10(6) to 23.9 x 10(6) oocysts. Observed overall mean log(10) reduction of total C. parvum flux per meter of vegetated buffer was 1.44, 1.19, and 1.18 for buffers at 5, 12, and 20% land slope, respectively. Rainfall application rate (mm/h) was strongly associated with oocyst flux from these vegetated buffers, resulting in a decrease of 2 to 4% in the log(10) reduction per meter buffer for every additional mm/h applied to the soil box. These results support the use of strategically placed vegetated buffers as one of several management strategies that can reduce the risk of waterborne C. parvum attributable to extensive cattle grazing on annual grassland watersheds.     

Incidence and persistence of zoonotic bacterial and protozoan pathogens in a beef cattle feedlot runoff control vegetative treatment system

Determining the survival of zoonotic pathogens in livestock manure and runoff is critical for understanding the environmental and public health risks associated with these wastes. The occurrence and persistence of the bacterial pathogens Escherichia coli O157:H7 and Campylobacter spp. in a passive beef cattle feedlot runoff control-vegetative treatment system were examined over a 26-mo period. Incidence of the protozoans Cryptosporidium spp. and Giardia spp. was also assessed. The control system utilizes a shallow basin to collect liquid runoff and accumulate eroded solids from the pen surfaces; when an adequate liquid volume is attained, the liquid is discharged from the basin onto a 4.5-ha vegetative treatment area (VTA) of bromegrass which is harvested as hay. Basin discharge transported E. coli O157, Campylobacter spp., and generic E. coli into the VTA soil, but without additional discharge from the basin, the pathogen prevalences decreased over time. Similarly, the VTA soil concentrations of generic E. coli initially decreased rapidly, but lower residual populations persisted. Isolation of Cryptosporidium oocysts and Giardia cysts from VTA samples was infrequent, indicating differences in sedimentation and/or transport in comparison to bacteria. Isolation of generic E. coli from freshly cut hay from VTA regions that received basin discharge (12 of 30 vs. 1 of 30 control samples) provided evidence for the risk of contamination; however, neither E. coli O157 or Campylobacter spp. were recovered from the hay following baling. This work indicates that the runoff control system is effective for reducing environmental risk by containing and removing pathogens from feedlot runoff.     

CONTRIBUTIONS OF RAINFALL TO CONSTITUENT LOADS IN STORM RUNOFF FROM URBAN CATCHMENTS1

ABSTRACT: Rainfall is a significant source of some constituents, particularly nitrogen species, in storm runoff from urban catchments. Median contributions of rainfall to storm runoff loads of 12 constituents from 31 urban catchments, representing eight geographic locations within the United States, ranged from 2 percent for suspended solids to 74 percent for total nitrite plus nitrate nitrogen. The median contribution of total nitrogen in rainfall to runoff loads was 41 percent. Median contributions of total-recoverable lead in rainfall to runoff loads varied by as much as an order of magnitude between catchments in the same geographic location. This indicates that average estimates of rainfall contributions to constituent loading in storm runoff may not be suitable in studies requiring accurate constituent mass-balance computations.     

Management of Urban Road Runoff Containing PAHs: Probabilistic Modeling and Its Application in Beijing,China1

Abstract: Pollutant loading from storm runoff is considered to be an important component of nonpoint source pollution in urban areas. In developing countries, because of the accelerated urbanization and motorization, storm runoff pollution has become a challenge for improving aquatic environmental quality. An effective storm runoff management plan needs to be developed, and questions concerning how much and which proportion of a storm should be treated need to be answered. In this study, a model is developed to determine the fraction of storm runoff that needs to be treated to meet the discharge standard within a given probability. The model considers that the pollutants can be mobilized during the early stage of a storm. The model is applied to a field study of polycyclic aromatic hydrocarbons (PAHs) in road runoff in Beijing, China. In this case, the probability that the PAH load will be mobilized with suspended sediments by the earlier portion of the flush is 73%. Given the high PAH loading in the study area and the referenced discharge standard, the probability that the entire runoff should be captured and treated is 94%. Thus, urban planners need to consider treatment systems for the majority of the storms in this area, whether the PAH load is in the first flush or not. This methodology can be applied to other regions where PAH loads may result in different management outcomes.     

Recently planted vegetation strips reduce Giardia runoff reaching waterways

Current methods for tracking pathogens across farmland and into surrounding waterways via runoff are limited and typically have been developed using artificially created landscapes. No studies have investigated how Giardia in farm runoff moves across the landscape, despite high prevalence rates in dairy cattle (Bos taurus) worldwide. Here, we report the development of a field-based tracking method specific for Giardia movement in runoff and use this technique to compare the pathogen reduction capability of recently planted vegetation strips with bare soil strips cleared of vegetation. Such scenarios represent typical events in schemes to plant vegetation barriers aimed at reducing waterway contamination. A significant treatment effect was identified, with 26% fewer Giardia detected in runoff collected from the planted strip (P = 0.006). These results highlight the immediate benefit of pathogen removal to be gained from vegetation planting. The successful discrimination of treatment effects by this new technique will enable the assessment of different vegetation types on runoff reduction and the effects of plant development over time.     

EFFECTS OF PRECIPITATION AND LAND USE ON STORM RUNOFF1

ABSTRACT: Storm-runoff quantity and quality were studied in three watersheds located near St. Paul in Ramsey County, Minnesota, from April 15 through September 15 of 1984, 1985, and 1986 to qualitatively determine the effects of precipitation and selected land uses on storm runoff. In respect to precipitation effects, differences in stormrunoff quantity between years in an urban watershed that lacks wetlands appear to be related to the average storm size (amount of precipitation) during the study period of each year. In contrast, the differences in storm-runoff quantity from watersheds that contain wetlands appear to be related to total precipitation during study period of each year. In respect to land use, the differences in storm-runoff quantity appear to be related to the amounts of impervious and wetland area. The watershed that contains the largest amount of impervious area and smallest amount of wetland area has the largest amount of storm runoff. Differences in storm-runoff quality appear to be related to the amounts of wetland and lake area. The watershed that contains the largest amounts of wetland and lake area has the smallest storm-runoff loading of suspended solids, phosphorus, and nitrogen. The wetland and lake areas likely retain the loading and, subsequently, lower the amount of storm-runoff loading exported from a watershed.     

Economic analysis of best management practices to reduce watershed phosphorus losses

In phosphorus-limited freshwater systems, small increases in phosphorus (P) concentrations can lead to eutrophication. To reduce P inputs to these systems, various environmental and agricultural agencies provide producers with incentives to implement best management practices (BMPs). In this study, we examine both the water quality and economic consequences of systematically protecting saturated, runoff-generating areas from active agriculture with selected BMPs. We also examine the joint water quality/economic impacts of these BMPs-specifically BMPs focusing on barnyards and buffer areas. Using the Variable Source Loading Function model (a modified Generalized Watershed Loading Function model) and net present value analysis (NPV), the results indicate that converting runoff-prone agricultural land to buffers and installing barnyard BMPs are both highly effective in decreasing dissolved P loss from a single-farm watershed, but are also costly for the producer. On average, including barnyard BMPs decreases the nutrient loading by about 5.5% compared with only implementing buffers. The annualized NPV for installing both buffers on only the wettest areas of the landscape and implementing barnyard BMPs becomes positive only if the BMPs lifetime exceeds 15 yr. The spatial location of the BMPs in relation to runoff producing areas, the time frame over which the BMPs are implemented, and the marginal costs of increasing buffer size were found to be the most critical considerations for water quality and profitability. The framework presented here incorporates estimations of nutrient loading reductions in the economic analysis, and is applicable to farms facing BMP adoption decisions.     

Hydrological variability and agricultural drainage ditch inorganic nitrogen reduction capacity

The application of inorganic nitrogen fertilizers on agricultural landscapes has the potential to generate concerns of environmental degradation at fine to coarse scales across the catchment and landscape. Inorganic nitrogen species (NO3*, NO2*, and NH3) are typically associated with subsurface flow processes; however, surface runoff from rainfall events in no-till agriculture with inorganic surface fertilizers might contribute to downstream eutrophication. Inorganic nitrogen reduction capacity of agricultural drainage ditches under no-till cotton was determined under natural, variable rainfall conditions in northern Mississippi. Monthly grab baseflow samples and storm-generated flow samples were variably sampled temporally within two experimental farm ditches over 2 yr. Inorganic nitrogen concentrations, in conjunction with Manning's equation and Natural Resources Conservation Service dimensionless hydrographs, provided individual water volumes per storm event and thus maximum effluent and outflow nitrogen loads. Base and stormflow regression results indicate drainage ditches reducing NO3* and NH3 over the length of the ditch for growing and dormant seasons. Overall, maximum storm loads of dissolved inorganic nitrogen (DIN) from the farm over the 2-yr sampling period accounted for 2.2% of the initial fertilizer application, of which 1.1% left the ditch (0.84 kg ha(-1) yr(-1)) (a 57% ditch reduction of DIN load over 2 yr). Long-term sampling incorporating data on application and loss of fertilizers and farm management will provide critical information for farmers and scientists on the potential of economic gains and downstream ecosystem eutrophication, respectively.     

TRANSPORT OF SEDIMENT-BOUND ORGANOCHLORINE PESTICIDES TO THE SAN JOAQUIN RWER,CALIFORNIA1

ABSTRACT: Suspended sediment samples were collected in west-side tributaries and the main stem of the San Joaquin River, California, in June 1994 during the irrigation season and in January 1995 during a winter storm. These samples were analyzed for 15 organochiorine pesticides to determine their occurrence and their concentrations on suspended sediment and to compare transport during the irrigation season (April to September) to transport during winter storm runoff (October to March). Ten organochiorine pesticides were detected during the winter storm runoff; seven during the irrigation season. The most frequently detected organochlorine pesticides during both sampling periods were p,p'-DDE, p,p'-DDT, p,p'-DDD, dieldrin, toxaphene, and chiordane. Dissolved samples were analyzed for three organochiorine pesticides during the irrigation season and for 15 during the winter storm. Most calculated total concentrations of p,p-DDT, chlordane, dieldrin, and toxaphene exceeded chronic criteria for the protection of freshwater aquatic life. At eight sites in common between sampling periods, suspended sediment concentrations and streamfiow were greater during the winter storm runoff - median concentration of 3,590 mg/L versus 489 mg(L and median streamfiow of 162 ft³/s versus 11 ft³/s. Median concentrations of total DDT (sum of p,p'-DDD, p,p-DDE, and p,p'-DDT), chlordane, dieldrin, and toxaphene on suspended sediment were slightly greater during the irrigation season, but instantaneous loads of organochlorine pesticides at the time of sampling were substantially greater during the winter storm. Estimated loads for the entire irrigation season exceeded estimated loads for the January 1995 storm by about 2 to 4 times for suspended transport and about 3 to 11 times for total transport. However, because the mean annual winter runoff is about 2 to 4 times greater than the runoff during the January 1995 storm, mean winter transport may be similar to irrigation season transport. This conclusion is tentative primarily because of insufficient information on long-term seasonal variations in suspended sediment and organochlorine concentrations. Nevertheless, runoff from infrequent winter storms will continue to deliver a significant load of sediment-bound organochiorine pesticides to the San Joaquin River even if irrigation-induced sediment transport is reduced. As a result, concentrations of organochlorine pesticides in San Joaquin River biota will continue to be relatively high compared to other regions of the United States.     

Results of a program to control phosphorus discharges from dairy operations in south-central Florida,USA

During 1987–1992, a mandatory program to control phosphorus discharges was implemented at dairy operations located to the north of Lake Okeechobee, Florida, USA. Thirty of 48 dairies participated in this program and implemented best management practices (BMPs), which included the construction of intensive animal waste management systems. Eighteen dairies closed their milkproducing operations under a government-funded buyout program. In this paper, we compare trends in runoff total phosphorus (TP) concentrations among the dairies that remained active and implemented BMPs. A central feature of the dairy waste management system is the high intensity area (HIA), defined as the milking barn and adjacent vegetation-free land, encircled by a drainage ditch and dike. Animal waste from the HIA is diverted into anaerobic lagoons and storage ponds, from which water is periodically removed and used for irrigation of field crops. The impacts of BMP construction on runoff TP concentrations were immediate and, in most cases, dramatic. Average TP concentrations declined significantly (P < 0.001), from 9.0 to 1.2 mg TP liter^–1 at dairies in one basin (Lower Kissimmee River), and from 2.6 to 1.0 mg TP liter^–1 in another (Taylor Creek/Nubbin Slough). Some sites experienced greater declines in TP than others. To elucidate possible causes for the difference in response, a multivariate statistical model was utilized. Independent variables included soil pH, soil drainage characteristics, spodic horizon depth, and the areas of different BMP components (pasture, HIA, spray fields). The analysis significantly separated dairies with the highest and lowest runoff TP concentrations. Lowest TP occurred at dairies having particular soil characteristic (shallow spodic horizon) and certain BMP features (large HIA and small heard pastures).     

Phosphorus, sediment, and Escherichia coli loads in unfenced streams of the Georgia Piedmont, USA

Contamination of unfenced streams with P, sediments, and pathogenic bacteria from cattle (Bos taurus) activity may be affected by the availability of shade and alternative water sources. The objectives of this study were to evaluate water quality in two streams draining tall fescue (Festuca arundinacea Schreb.)-common bermudagrass (Cynodon dactylon L.) pastures with different shade distribution, and to quantify the effects of alternative water sources on stream water quality. For 3 yr, loads of dissolved reactive phosphorus (DRP), total phosphorus (TP), and total suspended solids (TSS) were measured during storm flow, and loads of DRP, TP, TSS, and Escherichia coli were measured every 14 d during base flow. We also used GPS collars to determine amount of time cattle spent in riparian areas. Our results showed that cattle-grazed pastures with unfenced streams contributed significant loads of DRP, TP, TSS, and E. coli to surface waters (p < 0.01). Time spent by cattle in riparian areas as well as storm flow loads of DRP, TP, and TSS were larger (p < 0.08) in the pasture with the smaller amount of nonriparian shade. Water trough availability decreased base flow loads of TSS and E. coli in both streams, and decreased time cattle spent in riparian areas in the pasture with the smaller amount of nonriparian shade (p < 0.08). Our results indicate that possible BMPs to reduce contamination from cattle-grazed pastures would be to develop or encourage nonriparian shade and to provide cattle with alternative water sources away from the stream.