Optimisation of pressurised liquid extraction for elimination of sulphur interferences during determination of organotin compounds in sulphur-rich sediments by gas chromatography with flame photometric detection

A simple method for species-selective analysis of organotin compounds (OTCs) (butyl and phenyl) in sediments was developed. The sample preparation procedure was specifically optimised for sulphur-rich sediments to eliminate interferences from elemental sulphur and organosulphur compounds. Tin species were extracted from sediment samples using pressurised liquid extraction technique (PLE), ethylated - with simultaneous extraction to isooctane - in aqueous phase with sodium tetraethylborate (NaBEt(4)) and separated/detected by gas chromatography with flame photometric detection (GC-FPD). PLE operational variables (extraction temperature and pressure, solvent composition and number of static extraction steps) and extract handling routine were fine-tuned to minimise the amount of extracted interferents while keeping OTCs recovery at an acceptable level. Best results were obtained after extraction of sediment samples with methanol/water (75% v/v methanol) solution of acetic acid/sodium acetate with tropolone addition (0.6 g l(-1)). Derivatisation of low temperature, high-pressure (50 degrees C, 13.8 MPa) extracts gives isooctane extracts which are clean enough to be directly analysed by GC-FPD without any further cleanup. Interferences from elemental sulphur were completely eliminated while concentrations of other interferents were reduced to the level not impairing quantitation of OTCs under the study. No negative effects in terms of chromatographic column deterioration were observed after repeated injections of such extracts. Two certified reference materials, BCR646 and PACS-2, were analysed to assess performance of the method. Recoveries of all OTCs under the study, except MBT, were in the range of 91-114%. MBT extraction efficiency was low (34-47%) therefore the method is unsuitable for precise determinations of this compound.     

Effects of three pesticides on the avoidance behavior of earthworms in laboratory tests performed under temperate and tropical conditions

Little research has been performed on the impact of pesticides on earthworms under tropical conditions. Taking into consideration the often-limited resources in tropical countries, simple screening tests are needed. Therefore, it was investigated whether three pesticides relevant for the Brazilian Amazon (benomyl, carbendazim, lambda-cyhalothrin) affect the avoidance behavior of the earthworm Eisenia fetida. The tests were performed for two days according to ISO guideline 17512 but were adapted to tropical conditions (i.e. test substrate, test organism and temperature). The results indicate that this test gives reproducible and reliable results. Toxicity values (NOEC, EC50) are lower than those determined in 14 day-acute mortality tests and are approximately in the same range such as those found in 56 day-chronic reproduction tests with the same earthworm species, which were performed in parallel. Therefore, the use of the earthworm avoidance tests is recommended as a screening tool for the risk assessment of pesticides.     

一种改进的多学习教与学优化算法

针对传统教与学算法在解决复杂多峰函数优化问题时,具有局部最优且搜索开发能力较差的缺点,提出了一种改进的多学习教与学优化算法,新算法为学员的每一维加入不同的教学因子,设计了基于学员均值比较的教师选择策略和向教师及学员学习的多学习策略。基于多个单峰和多峰函数的仿真结果表明,新算法跟传统的、改进的教与学算法相比,在稳定性、寻优精度和收敛速度方面更具优势。     

A life history continuum in the males of a Neotropical ant assemblage: refuting the sperm vessel hypothesis

Animal lifespans range from a few days to many decades, and this life history diversity is especially pronounced in ants. Queens can live for decades. Males, in contrast, are often assumed to act as ephemeral sperm delivery vessels that die after a brief mating flight—a view developed from studies of lekking species in temperate habitats. In a tropical ant assemblage, we found that males can live days to months outside the nest, a trait hypothesized to be associated with female calling, another common mating system. We combined feeding experiments with respirometry to show that lifespan can be enhanced over 3 months by feeding outside the nest. In one focal female calling species, Ectatomma ruidum, feeding enhanced male lifespan, but not sperm content. Extended lifespans outside the nest suggest stronger than expected selection on premating traits of male ants, although the ways these traits shape male mating success remain poorly understood.     

Adaptation Scenarios of Agriculture in Poland to Future Climate Changes

This paper demonstrates the ability of Polish agriculture to adapt to predicted climate change according to GISS and GFDL scenarios. Both climate-change scenarios will significantly affect farming conditions in Poland through water deficit, shifts in planting and harvesting seasons, changes in crop yields and crop structure. Neither scenario seems to endanger the self-sufficiency of Poland as long as preventative measures are taken. Moreover, the realization of GISS creates the possibility of a surplus in production. It must be emphasized that regardless of the scenario, the adaptation of agriculture to an expected climate change cannot be handled by the farming community itself.     

The use of multiple tracers for tracking wastewater discharges in freshwater systems

The assessment of potential impacts of wastewater effluent discharges in freshwater systems requires an understanding of the likely degrees of dilution and potential zones of influence. In this study, four tracers commonly present in wastewater effluents were monitored to compare their relative effectiveness in determining areas in freshwater systems that are likely to be impacted by effluent discharges. The four tracers selected were the human pharmaceutical carbamazepine, anthropogenic gadolinium, fluorescent-dissolved organic matter (fDOM), and electrical conductivity (EC). The four tracers were monitored longitudinally in two distinct freshwater systems receiving wastewater effluents, where one site had a high level of effluent dilution (effluent <1 % of total flow) and the other site had a low level of effluent dilution (effluent ～50 % of total flow). At both sites, the selected tracers exhibited a similar pattern of response intensity downstream of discharge points relative to undiluted wastewater effluent, although a number of anomalies were noted between the tracers. Both EC and fDOM are non-specific to human influences, and both had a high background response, relative to the highly sensitive carbamazepine and anthropogenic gadolinium responses, although the ease of measuring EC and fDOM would make them more adaptable in highly variable systems. However, the greater sensitivity and selectivity of carbamazepine and gadolinium would make their combination with EC and fDOM as tracers of wastewater effluent discharges highly desirable to overcome potential limitations of individual tracers.     

Remobilization of polychlorinated biphenyls from sediment and its consequences for their transport in river waters

A laboratory experiment was performed to examine the remobilization of indicator polychlorinated biphenyls (iPCBs) from sediments and its results were applied to the real-world data for explaining the transport of PCBs in river. Seven PCB concentrations were determined in three series of model water–sediment systems (3 g of river sediment, three different volumes of distilled water (0.5, 0.25, and 0.15 ml), and 5 mg of biocide) after 11 days of incubation. Solid-phase extraction was used for separation of analytes from the aqueous phase and solvent extraction for isolation of analytes from the sediments, respectively. The extracts were analyzed for individual iPCB congeners using gas chromatography–mass spectrometry method. For each series of the experiment, the concentrations of PCBs in aqueous phase were similar. The average sediment/water partition coefficient value was 10⁴?l/kg. The solubility of individual PCB congeners in water did not influence the desorption of PCBs from the sediment. Although the dominant form of PCBs in a water–sediment system occurs as suspended and colloidal fractions, these compounds are transported mostly in a dissolved form. Suspended and colloidal matter is a major sink for PCBs in low-energy aquatic environments. In contrast, the dissolved PCBs are readily transported in running waters. The mobilization of PCBs from sediments to aqueous phase, with respect to their solubility in water, seems to be limited, thus reducing the risk of secondary pollution.     

Habitat monitoring and genotoxicity in Ucides cordatus (Crustacea: Ucididae), as tools to manage a mangrove reserve in southeastern Brazil

In Brazil, the state of São Paulo contains both preserved areas (Juréia-Itatins Ecological Station) and extremely impacted ones (Cubatão Municipality). This study evaluated the concentrations of five metals (Cu, Cd, Cr, Pb, and Hg) in two mangroves with different levels of anthropogenic impact and the apparent genotoxicity to Ucides cordatus. Water and sediment samples were obtained, and metal concentrations were determined with an atomic absorption spectrophotometer. The genotoxic impact was quantified based on the number of micronucleated cells per 1,000 analyzed (MN‰), using hemolymph slides stained with Giemsa. Metal concentrations in water were below the detection limit, except for lead, although no significant difference was observed between the areas (P?>?0.05). Sediment from Cubatão had higher concentrations of Cd, Pb, Cr, and Cu than sediment from Juréia-Itatins (P?<?0.05), but no significant differences in metal concentrations were detected among depth strata of the sediment (P?>?0.05). Crabs from Cubatão had a 2.6 times higher mean frequency of micronucleated cells (5.2?±?1.8 MN‰) than those from Juréia-Itatins (2.0?±?1.0 MN‰; P?<?0.0001). The more-polluted conditions found in the mangrove sediments of Cubatão were reflected in the micronucleus assay, demonstrating their genotoxic effect; however, genetic damage should be attributed to a synergistic effect with other kinds of pollutants previously recorded in different environments of Cubatão. U. cordatus proved to be an excellent bioindicator of mangrove pollution. This study established, for the first time, the normal frequency of MN‰ in a population of this species within an ecological station.