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Jane C. Chuang Jeanette M. Van Emon Margaret E. Tefft Nancy K. Wilson 《Journal of environmental science and health. Part. B》2013,48(6):516-523
A low-cost, high throughput bioanalytical screening method was developed for monitoring cis/trans-permethrin in dust and soil samples. The method consisted of a simple sample preparation procedure [sonication with dichloromethane followed by a solvent exchange into methanol:water (1:1)] with bioanalytical detection using a magnetic particle enzyme-linked immunosorbent assay (ELISA). Quantitative recoveries (83–126 %) of cis/trans-permethrin were obtained for spiked soil and dust samples. The percent difference of duplicate ELISA analyses was within ± 20 % for standards and ± 35 % for samples. Similar sample preparation procedures were used for the conventional gas chromatography/mass spectrometry (GC/MS) analysis except that additional cleanup steps were required. Recoveries of cis/trans-permethrin ranged from 81 to 108 % for spiked soil and dust samples by GC/MS. The ELISA-derived permethrin concentrations were highly correlated with the GC/MS-derived sum of cis/trans-permethrin concentrations with a correlation coefficient (r) of 0.986. The ELISA method provided a rapid qualitative screen for cis/trans-permethrin in soil and dust while providing a higher sample throughput with a lower cost as compared to the GC/MS method. The ELISA can be applied as a complementary, low-cost screening tool to prioritize and rank samples prior to instrumental analysis for exposure studies. 相似文献
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An enzyme-linked immunosorbent assay for the determination of dioxins in contaminated sediment and soil samples 总被引:1,自引:1,他引:0
A 96-microwell enzyme-linked immunosorbent assay (ELISA) method was evaluated to determine PCDDs/PCDFs in sediment and soil samples from an EPA Superfund site. Samples were prepared and analyzed by both the ELISA and a gas chromatography/high resolution mass spectrometry (GC/HRMS) method. Comparable method precision, accuracy, and detection level (8 ng kg(-1)) were achieved by the ELISA method with respect to GC/HRMS. However, the extraction and cleanup method developed for the ELISA requires refinement for the soil type that yielded a waxy residue after sample processing. Four types of statistical analyses (Pearson correlation coefficient, paired t-test, nonparametric tests, and McNemar's test of association) were performed to determine whether the two methods produced statistically different results. The log-transformed ELISA-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin values and log-transformed GC/HRMS-derived TEQ values were significantly correlated (r=0.79) at the 0.05 level. The median difference in values between ELISA and GC/HRMS was not significant at the 0.05 level. Low false negative and false positive rates (<10%) were observed for the ELISA when compared to the GC/HRMS at 1,000 ng TEQ kg(-1). The findings suggest that immunochemical technology could be a complementary monitoring tool for determining concentrations at the 1,000 ng TEQ kg(-1) action level for contaminated sediment and soil. The ELISA could also be used in an analytical triage approach to screen and rank samples prior to instrumental analysis. 相似文献
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Increased risk of preterm delivery among people living near the three oil refineries in Taiwan 总被引:1,自引:0,他引:1
The petrochemical and petroleum industries are two of the main sources of industrial air pollution in Taiwan. Data used in this study concern outdoor air pollution and the health of individuals living in communities in close proximity to oil refinery plants. The prevalence of delivery of preterm birth infants was significantly higher in mothers living near the oil refinery plants than in control mothers in Taiwan. After controlling for several possible confounders (including maternal age, season, marital status, maternal education, and infant sex), the adjusted odds ratio was 1.14 (95% CI=1.01-1.28) for delivery of preterm infants for mothers living near oil refinery plants. These data provide further support for the hypothesis that air pollution can affect the outcome of pregnancies. 相似文献
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经历了十几年的讨论,燃油税改革方案终于在2009年出台并实施,改革后燃油税究竟会对我国宏观经济及各部门产生怎样的影响,倍受关注.基于此背景,采用环境CGE模型对改革前后的两种不同燃油税税率进行了量化分析,总体研究得出:从长期发展来看,燃油税改革有利于我国经济结构调整,减少能源消耗和降低碳排放;短期内会增加企业生产成本,抑制宏观经济增长,其中交通运输业和石油加工业受到的冲击最大,应引起有关部门重视. 相似文献
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鉴于组织安全文化评估过程中,各因素指标及其相互作用关系具有模糊性,引入模糊数学理论,提出一种基于模糊贴近度的组织安全文化评估(SCA)方法。首先,构建组织安全文化因素指标体系以及确定SCA特征状态模式;其次,依据模糊语言隶属度函数以及特征状态模式,确定指标与SCA等级的隶属度;第三,确定指标与各等级的非对称贴近度,依据模糊贴近度判断矩阵进行评估决策;最后,以核电组织安全文化为例,通过对比分析进行方法的验证。实例分析结果的一致性表明:该方法能有效量化系统状态与各评估等级之间的贴近程度,能解决安全文化以及评估状态的模糊性问题,且评估结果与实际状态相吻合。 相似文献