Genotoxicity of methyl parathion in short‐term bacterial test systems

Abstract

Genotoxicity of the insecticide methyl parathion was investigated in Salmonella typhimurium and Escherichia coli bacterial test systems for the detection of back mutations and DNA‐damage. Methyl parathion was mutagenic to S. typhimurium strain TA100 after activation with rat liver microsomal and cytosolic enzymes. In DNA repair tests, methyl parathion was effective in inducing damage to the S. typhimurium strain TA1538 which lack excision repair compared to the strain TA1978 which is proficient in excision repair mechanisms. Normal laboratory light conditions had no effect on the mutagenicity tests, however, exposure of methyl parathion in the petri dish containing the tester strain TA100 and rat liver microsomal and cytosolic enzymes reduced the mutagenic activity and increased the toxic effects of methyl parathion.     

Speciative distribution and bioavailability of metals in agricultural soils receiving industrial wastewater

In order to assess the metal pollution status of agricultural lands of Mandi Bahauuddin receiving industrial wastewater, 35 top soil samples were investigated for the determination of selected metal levels, i.e., Fe, Cu, Cd, Cr, Ca, Ni, and Pb by flame atomic absorption spectroscopy under optimum analytical conditions. The distribution of these metals in different operationally defined chemical fractions was also determined by using the sequential extraction technique. The highest mean total concentration was found for Fe while the least one was observed for Pb. All the studied metals were found to be present at levels much enhanced than national and international standards. Moreover, most of the metals were distributed principally in residual fraction with the exception of Ni which was found to be associated mainly with oxidizable fraction. The significant correlations were observed between Fe-Mn oxide-bound and residual fractions and exchangeable and oxidizable fractions for most of the metals. The highest mobility was exhibited by Ni that evidenced its enhanced bioavailability in the soil. The multivariate statistical analyses in terms of principal component analysis (PCA) and cluster analysis (CA) revealed multiple sources for various geochemical fractions of different metals. CA also revealed that the nonresidual fractions of most of the metals were very closely associated while PCA presented a distinctive behavior of Ca in the soil. It was therefore suggested that in order to avoid the metal contamination arising from industrial wastewater, appropriate remediation strategies must be adopted.     

Evaluation of Beta‐naphthoxyacetic Acid for Mutagenic Activity in the Salmonella/mammalian Microsome Assay

1,3‐β‐d‐Glucan derived from baker's yeast was chemically modified in two steps yielding crosslinked carboxymethyl glucan as the sodium salt (2). After cation exchange with hexadecyltrimethylammonium chloride, a hydrophobic adsorbent (3) was obtained which showed an excellent binding of the estrogenic mycotoxin zearalenone with a maximum adsorption of up to 183 mg/g. Compound 3 additionally showed a relatively high adsorption capacity for the trichothecene T‐2 toxin of at least 10 mg/g. Starting from 2, various derivatives were prepared by cation exchange using quaternary ammonium salts bearing substituents besides methyl from four to 18 carbon atoms. The adsorption of T‐2 toxin on these derivatives were compared with compound 3 leading to the conclusion that 3 is the best adsorbent of all investigated tetraalkylammonium‐modified derivatives of 2.     

Reactions between aqueous zinc,aluminium and iron with increasing solutionpH

Solutions of zinc, aluminium and/or iron, as chlorides in NaCl of ionic strength 0.13 mol/l atpH 3, were mixed to give initial concentrations of 0–5, 5–50 and 10–100 g/ml, respectively. Sodium hydroxide was then added to increase thepH in a range up to 7. Turbidity was measured and equilibrium concentrations were determined after filtration (< 0.1 m). Decrease inpH on mixing suggested an immediate reaction, with the release of protons. At allpH values Zn concentration decreased with increasing initiai concentrations of Fe and Al. Adsorption and/or nucleation of Zn with Fe and Al was highlypH dependent and increased markediy with increasingpH. The particle size of the products increased with increasingpH and initial concentration of the elements. Increasing Al concentration decreased the size of Fe nucleates at a given Fe concentration. The results suggest that co-precipitation may be involved as well as adsorption. This has important consequences for the availability of Zn to plants, especially in Zn-deficient soils, and is of relevance to the products of mixing acid drainage waters with streams and lakes.     

Evaluation of chlordimeform and degradation products for mutagenic and DNA-damaging activity in Salmonella typhimurium and Escherichia coli

The mutagenic activity of chlordimeform and two of its breakdown products, 4-chloro-o-toludine and 4-chloro-N-formyl-o-toluidine were determined with five histidine dependent strains of Salmonella typhimurium (TA1535, TA1537, TA1538, TA98, TA100) and five tryptophan dependent strains of E. coli WP2 (WP2, WP2uvrA, WP67, CM611, CM571) with and without rat liver microsomal enzymes. 4-chloro-o-toluidine increased the number of the reversions of the S. typhimurium strain TA1535 more than two fold over spontaneous at the concentration of 400 micrograms/plate. The results of the DNA repair tests in the Salmonella TA1538/TA1978 and E. coli multirepair deficient systems showed that both breakdown products were active in inducing damage not repaired in at least one repair deficient strain while chlordimeform itself was inactive.     

Impact of anthropogenic activities on water quality of Lidder River in Kashmir Himalayas

The pristine waters of Kashmir Himalaya are showing signs of deterioration due to multiple reasons. This study researches the causes of deteriorating water quality in the Lidder River, one of the main tributaries of Jhelum River in Kashmir Himalaya. The land use and land cover of the Lidder catchment were generated using multi-spectral, bi-seasonal IRS LISS III (October 2005 and May 2006) satellite data to identify the extent of agriculture and horticulture lands that are the main non-point sources of pollution at the catchment scale. A total of 12 water quality parameters were analyzed over a period of 1 year. Water sampling was done at eight different sampling sites, each with a varied topography and distinct land use/land cover, along the length of Lidder River. It was observed that water quality deteriorated during the months of June–August that coincides with the peak tourist flow and maximal agricultural/horticultural activity. Total phosphorus, orthophosphate phosphorus, nitrate nitrogen, and ammoniacal nitrogen showed higher concentration in the months of July and August, while the concentration of dissolved oxygen decreased in the same period, resulting in deterioration in water quality. Moreover, tourism influx in the Lidder Valley shows a drastic increase through the years, and particularly, the number of tourists visiting the valley has increased in the summer months from June to September, which is also responsible for deteriorating the water quality of Lidder River. In addition to this, the extensive use of fertilizers and pesticides in the agriculture and horticulture lands during the growing season (June–August) is also responsible for the deteriorating water quality of Lidder River.     

OPTIMAL MANAGEMENT OF A REGIONAL AQUIFER IN EASTERN SAUDI ARABIA1

ABSTRACT: This paper describes the formulation and application of a ground-water hydraulic management model to determine the optimal development and operating policies of a regional aquifer in the Eastern Province of Saudi Arabia. The hydraulic response of the aquifer system is represented by a simulation model that is linked to an optimization management model using response functions. Yearly optimal ground-water extraction rates over a planning horizon of 15 years are determined for four scenarios, each reflecting alternative ground-water development policies. The results are presented in the form of tradeoff curves, relating drawdowns to optimal pumpage, which may enhance the decisionmaker's ability to select the best development policy from a set of alternatives. The results illustrate how various optimal management schemes can be devised to increase the total withdrawal from the aquifer while preventing excessive de-watering.     

Field-scale application of oily food waste and nitrogen fertilizer requirements of corn at different landscape positions

Oily food waste (FOG; fat + oil + greases) containing high concentrations of fat, oil and grease is produced by the food service, production, and processing industries. It has a high C to N ratio (90:1) and can recycle soil available N through immobilization and remineralization during its decomposition. Experiments were conducted at a farm (Hillsburg fine sandy loam; Typic Hapludalf) having rolling topography (5 and 9% slope) during 1995 and 1996. Objectives of this study were to (i) examine the variability of available N and corn (Zea mays L.) grain yield at different landscape positions of FOG-amended fields and (ii) determine whether N fertilizer management could be improved by considering the spatial variability of soil NO(3)-N at different landscape positions in FOG-amended fields. A spatial and temporal variability in soil NO(3)-N was observed during both years. Corn grain yields at all N fertilizer application rates were affected by slope position and followed the pattern: lower > upper > or = middle. Nitrogen fertilizer requirements for corn production in conjunction with FOG management were also affected by slope position. Essentially no additional fertilizer N was required for corn production at the lower landscape position. It was estimated that site-specific fertilizer N management on FOG-amended fields could result in an average savings of 51 and 63 kg N ha(-1) (with a potential economical savings of US 42 dollars and US 52 dollars ha(-1)) during 1995 and 1996, respectively.     

Needs-based sewerage prioritization: alternative to conventional cost-benefit analysis

This paper presents an empirical approach to select and prioritize sewerage projects within set budgetary limitations. The methodology includes a model which quantifies benefits of a sewerage project as an index or dimensionless number. The index considers need and urgency of sewerage and other project goals. Benefit is defined as the difference in anticipated impact between the current condition (without the project) and the expected condition with the project. Anticipated benefits primarily include reduction in environmental pollution, reduction of human diseases and morbidity, and other tangible and intangible improvement. This approach is a powerful decision tool for sewerage prioritization and an effective alternative to conventional cost-benefit analysis. Unlike conventional analysis, this approach makes no attempt to convert project benefits and other impacts into a monetary measure. This work recognizes that the decision to provide sewerage based solely on net benefits is not practical. Instead, benefit-cost ratios (B/C) are calculated utilizing cost-effectiveness approach. Using these ratios, 16 unserviced areas of Ensenada, Mexico are ranked. The prioritization rankings produced by this method must be further scrutinized and carefully reviewed for logic, accuracy of input data, and practicality of implementation. A similar framework may also be useful for prioritizing other public works projects.