The use of gas chromatography with ion-trap MS/MS detection for the determination of planar PCBs in biota and sediment

Combining capillary GC with ion-trap detection in the MS/MS mode allows the determination of trace levels of the highly toxic PCB congeners, CB 77, 126 and 169 in complex biological tissues, suspended matter and sediment. The high selectivity and sensitivity of MS/MS detection in the resonant and non-resonant modes yield detection limits of 60 fg, 300 fg and 200 fg for CB 77, 126, and 169, respectively.     

Bioavailability to rats of bound [14C] pirimiphos-methyl in stored wheat.

Stored wheat treated with radiolabelled pirimiphos-methyl (0-2-diethyl-amino-6-methyl-pyrimidin-4-yl 0,0-dimethyl phosphorothioate) formed bound (nonextractable) 14C residues. Supercritical fluid extraction, gas chromatography and mass spectrometric techniques were used to identify and quantitate the 14C bound residues in wheat grains. The amount of bound 14C residues present after 28 weeks of storage was about 9.9% of the applied radioactivity. Pirimiphos-methyl accounted for 80% of the bound residue. Grain-bound residues were fed to rats for 5 days. After a total period of 8 days a substantially large percentage of the administered bound 14C residues (72.9%) was eliminated in urine while feces contained only 17.9%. Bound pirimiphos-methyl in wheat grain was metabolized in rats by processes involving hydrolysis, N-dealkylation and 0-demethylation. The results indicate that wheat-bound residues of pirimiphos-methyl are highly bioavailable to the rat and may possess a toxicological potential as manifested by a significant reduction in body weight gain.