Bioproduction strategies for rare hexose sugars

A new strategy for the bioproduction of all ketohexoses was developed using hexitols as intermediates. Biocatalysts used to employ the strategy were D-tagatose 3-epimerase, which epimerizes ketohexoses at the C-3 position, and oxidoreductases, which catalyze oxidation-reduction reactions between ketohexoses and the corresponding hexitols. Arranging all the ketohexoses and hexitols in a symmetric ring and connecting them with 20 biochemical reactions, I was able to construct a design for the bioproduction of all the rare ketohexoses. Various aldose isomerases transform ketohexoses into the corresponding aldohexoses, so the strategy is useful for the bioproduction of all the rare hexose sugars. Furthermore, the design revealed that there are four routes to the L-hexose world from the D-hexose one.     

Climatic and stream-flow controls on tree growth in a Western montane riparian forest

Humans have severely impacted riparian ecosystems through water diversions, impoundments, and consumptive uses. Effective management of these important areas is becoming an increasingly high priority of land managers, particularly as municipal, industrial, and recreational demands for water increase. We examined radial tree growth of four riparian tree species (Pinus jeffreyi, Populus trichocarpa, Betula occidentalis, and Pinus monophylla) along Bishop Creek, California, and developed models relating basal area increment (BAI) and relative basal area increment (RBAI) to climatic and stream flow variables. Between years 1995–1999, univariate regression analysis with stream flow explained 29 to 61% of the variation in BAI and RBAI among all species except P. trichocarpa; growth by P. trichocarpa was not significantly related to stream flows over this period. Stepwise linear regression indicated that species responded differently to climatic variables, and models based on these variables explained between 33 to 86% of variation in BAI and RBAI during the decade of the 1990s. We examined branch growth of P. trichocarpa for sensitivity to differences in stream flow regimes and found that annual branch growth did not vary between a high- and low-flow site, but that annual branch growth was significantly higher in wet years with greater stream flows. Our results support the establishment of site-specific management goals by land managers that take into account all of the important tree species present in riparian ecosystems and their differential responses to altered hydrologic condition. Instream flow requirements for maintaining tree growth and vigor are only one of the species-specific responses that need to be evaluated, and these assessments should attempt to separate experimentally stream-flow (managed) controls from climatic (unmanaged) controls on growth.     

Land-cover change in upper Barataria Basin estuary,Louisiana, 1972-1992: increases in Wetland area

The Barataria Basin, Louisiana, USA, is an extensive wetland and coastal estuary system of great economic and intrinsic value. Although high rates of wetland loss along the coastal margin of the Barataria Basin have been well documented, little information exists on whether freshwater wetlands in the upper basin have changed. Our objectives were to quantify land-cover change in the upper basin over 20 years from 1972–1992 and to determine land-cover transition rates among land-cover types. Using 80-m resolution Landsat MSS data from the North American Landscape Characterization (NALC) data archive, we classified images from three time steps (1972, 1985, 1992) into six land-cover types: agriculture, urban, bottomland hardwood forest, swamp forest, freshwater marsh, and open water. Significant changes in land cover occurred within the upper Barataria Basin over the study period. Urban land increased from 8% to 17% of the total upper basin area, primarily due to conversions from agricultural land, and to a lesser degree, bottomland forest. Swamp forest increased from 30% to 41%, associated with conversions from bottomland hardwood forest and freshwater marsh. Overall, bottomland forest decreased 38% and total wetland area increased 21%. Within the upper Barataria, increases in total wetland area may be due to land subsidence. Based on our results, if present trends in the reduction of bottomland forest land cover were to continue, the upper Barataria Basin may have no bottomland hardwood forests left by the year 2025, as it is subjected to multiple stressors both in the higher elevations (from urbanization) and lower elevations (most likely from land subsidence). These results suggest that changes in the upper freshwater portions of coastal estuaries can be large and quite different from patterns observed in the more saline coastal margins.     

Characterization of humic-rich hydrocolloids and their metal species by means of competing ligand and metal exchange--an on-site approach

An improved on-site characterization of humic-rich hydrocolloids and their metal species in aquatic environments was the goal of the present approach. Both ligand exchange with extreme chelators (diethylenetetraaminepentaacetic acid (DTPA), ethylendiaminetetraacetic acid (EDTA)) and metal exchange with strongly competitive cations (Cu(II) were used on-site to characterize the conditional stability and availability of colloidal metal species in a humic-rich German bogwater lake (Venner Moor, Münsterland). A mobile time-controlled tangential-flow ultrafiltration technique (cut-off: 1 kDa) was applied to differentiate operationally between colloidal metal species and free metal ions, respectively. DOC (dissolved organic carbon) and metal determinations were carried out off-site using a home-built carbon analyzer and conventional ICP-OES (inductively-coupled plasma-optical emission spectrometry), respectively. From the metal exchange equilibria obtained on-site the kinetic and thermodynamic stability of the original metal species (Fe, Mn, Zn) could be characterized. Conditional exchange constants Kex obtained from aquatic metal species and competitive Cu(II) ions follow the order Mn > Zn > > Fe. Obviously, Mn and Zn bound to humic-rich hydrocolloids are very strongly competed by Cu(II) ions, in contrast to Fe which is scarcely exchangeable. The exchange of aquatic metal species (e.g. Fe) by DTPA/EDTA exhibited relatively slow kinetics but rather high metal availabilities, in contrast to their Cu(II) exchange.     

Concentration and size distribution of ultrafine particles near a major highway

Motor vehicle emissions usually constitute the most significant source of ultrafine particles (diameter <0.1 microm) in an urban environment, yet little is known about the concentration and size distribution of ultrafine particles in the vicinity of major highways. In the present study, particle number concentration and size distribution in the size range from 6 to 220 nm were measured by a condensation particle counter (CPC) and a scanning mobility particle sizer (SMPS), respectively. Measurements were taken 30, 60, 90, 150, and 300 m downwind, and 300 m upwind, from Interstate 405 at the Los Angeles National Cemetery. At each sampling location, concentrations of CO, black carbon (BC), and particle mass were also measured by a Dasibi CO monitor, an aethalometer, and a DataRam, respectively. The range of average concentration of CO, BC, total particle number, and mass concentration at 30 m was 1.7-2.2 ppm, 3.4-10.0 microg/m3, 1.3-2.0 x 10(5)/cm3, and 30.2-64.6 microg/m3, respectively. For the conditions of these measurements, relative concentrations of CO, BC, and particle number tracked each other well as distance from the freeway increased. Particle number concentration (6-220 nm) decreased exponentially with downwind distance from the freeway. Data showed that both atmospheric dispersion and coagulation contributed to the rapid decrease in particle number concentration and change in particle size distribution with increasing distance from the freeway. Average traffic flow during the sampling periods was 13,900 vehicles/hr. Ninety-three percent of vehicles were gasoline-powered cars or light trucks. The measured number concentration tracked traffic flow well. Thirty meters downwind from the freeway, three distinct ultrafine modes were observed with geometric mean diameters of 13, 27, and 65 nm. The smallest mode, with a peak concentration of 1.6 x 10(5)/cm3, disappeared at distances greater than 90 m from the freeway. Ultrafine particle number concentration measured 300 m downwind from the freeway was indistinguishable from upwind background concentration. These data may be used to estimate exposure to ultrafine particles in the vicinity of major highways.     

Measuring agricultural sustainability in terms of efficiency: the case of Dutch sugar beet growers

Sustainability embraces socio-economic and bio-ecological dimensions or attributes. This paper presents a conceptual framework for quantifying sustainability on the basis of efficiency theory commonly used in economics. The conceptual model is implemented using Data Envelopment Analysis (DEA). Sustainability is measured for a sample of Dutch sugar beet growers. The average technical efficiency was only 50%. A positive correlation was found between technical efficiency and sustainable efficiency. Differences in efficiency among farmers were persistent within and between years. We conclude that there is considerable scope for improving the sustainability of arable farming by better management.     

Evaluating Sediment Stability at Sites with Historic Contamination

The stability of cohesive sediment deposits during a rare storm is a critical component in the evaluation of remedial options at a contaminated sediment site. Estimating scour depths during a rare storm, and the resulting contaminant concentrations in the surficial layer of the bed, is necessary for comparing the efficacy of various remedial alternatives. Evaluation of sediment stability is accomplished using sediment transport analyses that employ quantitative procedures. Qualitative analyses or conceptual models can be useful for developing and validating quantitative analysis tools; however, qualitative techniques alone generally are insufficient for conducting defensible remedial alternative evaluations. The level of analysis used for a specific site depends on data availability, required level of accuracy, and time and budget constraints. A tier 1 analysis involves the use of approximate equations to produce order-of-magnitude estimates of scour depths during a rare storm. The second tier of this analysis scheme employs the development and application of a sediment transport model to evaluate bed stability. State-of-the-science sediment transport models have been effectively used as management tools for evaluating remedial options at several contaminated sediment sites. It should not be presumed that rare storm events cause catastrophic impacts at the site under review. Two case studies demonstrate that a rare storm is not necessarily catastrophic; significant increases in surficial bed concentrations caused by reexposure of elevated concentrations buried at depth in the bed will not necessarily occur during a rare storm. However, it is important to note that sediment stability is site-specific.     

Patterns of Streamwater Acidity in Lye Brook Wilderness,Vermont, USA

Under the United States Clean Air Act Amendments of 1977, a class I designation safeguards wilderness areas from the negative effects of new sources of air pollution. We monitored streamwater chemistry in the class I Lye Brook Wilderness in southwestern Vermont from May 1994 through August 1995. Stream samples were collected biweekly at nine sampling locations throughout the wilderness and were analyzed for major cations and anions, dissolved organic carbon, pH, and acid-neutralizing capacity. Eight of nine sites sampled had mean annual acid neutralizing capacity values below zero. During the study period, decreases in streamwater acid neutralizing capacity values were caused primarily by SO₄ ²⁻. At some sites, however, NO₃ ⁻ and naturally occurring, weak organic acids were seasonally important. During high discharge, the low pH and high concentrations of inorganic monomeric Al were at levels that are toxic to acid-sensitive aquatic species. Watershed mass balances were calculated to determine annual gains or losses for measured ions. These budgets indicate that S inputs and outputs were nearly equal, there was a net loss of base cations, and a net gain in N. How long these watersheds can continue to assimilate additional N inputs is unknown.     

Fertilizer Management in Watersheds of Two Ramsar Wetlands and Effects on Quality of Inflowing Water

Two field experiments were carried out in the watersheds of two Ramsar wetland areas, Lakes Koronia and Volvi (area A) and Lakes Mikri and Megali Prespa (area B), to study the effect of application of N fertilizer on wheat yields, the quality of runoff water, and the quality of stream water. The treatments were a combination of two methods of fertilizer application (total amount in fall, and 2/3 in fall + 1/3 in spring) at three rates (0, 100, and 200 kg N/ha) with four replications. Concentrations of NH₄ ⁺, NO₃ ⁻, NO₂ ⁻, P, and Cl⁻ and pH were determined in all water samples. Runoff water quality was not influenced by fertilizer application in either area. Chemical parameters for water did not differ along the selected watercourses in area B, while in area A they were higher in the samples taken near Lake Koronia than in the samples taken upstream, indicating that the watercourses are polluted downstream by nonagricultural sources. The differences in wheat yields between the 100 and 200 kg N/ha application rates were not high. These results call for better fertilizer management in order to achieve better yields and to diminish the possibility to have negative effects to the environment.     

Integrated economic-ecological analysis and evaluation of management strategies on nutrient abatement in the Rhine basin

Management of river basins involves the making of informed choices about the desired levels of economic activities and ecosystem functioning in the catchment. Information on the economic and ecological effects of measures as well as their spatial distribution is therefore needed. This paper proposes the following instruments to support decision-making in river basins: (1) the linking of models and indicators to describe the economic and ecological effects of management actions and their spatial distribution and (2) an extended evaluation framework that aims to evaluate management actions on three objectives for sustainable river management. These are cost-effectiveness, spatial equity, and environmental quality. This paper illustrates the potential of these instruments for river basin management by a case-study on nutrient management in the Rhine basin. In this case-study four nutrient abatement strategies are formulated, based on policies of the International Commission for the Protection of the Rhine and the North Sea Commission. These strategies are analysed and evaluated on their contribution to the three management objectives. Results show that none of these strategies score highest on cost-effectiveness, spatial equity and environmental quality simultaneously. It appears that cost-effectiveness is in conflict with environmental quality, whereas spatial equity and cost-effectiveness show quite close correspondence. This means that a trade-off has to be made between costs and spatial equity on the one hand, and environmental standards on the other hand. This paper offers a framework to make these trade-offs more explicit and provides quantitative information on cause-effect relationships, economic and environmental effects and the spatial distribution of these effects for various management strategies. This information can be particularly useful in the development of compromises required to establish international agreement and co-operation.