Limited scope for reproductive senescence in wild populations of a short-lived fish

Senescence in wild populations was long considered negligible but current evidence suggests that it is widespread in natural populations of mammals and birds, affecting the survival and reproductive output of older individuals. In contrast, little is known about reproductive senescence in species with asymptotic growth that can keep increasing their reproductive output as they grow older and larger. Using a cross-sectional study, we tested age-related decline in fecundity and relative allocation to reproduction in five wild populations of an annual killifish, Nothobranchius furzeri (Cyprinodontiformes). We did not detect any decline in absolute female egg production over their short lifespan in the wild. Relative fecundity (egg production controlled for female body mass) tended to decrease with age. This effect was driven primarily by a single population that survived 17 weeks, almost twice as long as the median persistence of the other four study populations. There was no decrease in relative ovary mass while in males, relative testes mass actually increased with age. Intra-population variation in relative ovary mass increased in older females suggesting heterogeneity in individual trajectories of female reproductive allocation. Overall, we demonstrate that annual killifish do not experience significant age-related decline in reproductive functions during their very short lifespan in the wild despite the marked deterioration of gonad tissue detected in captivity.     

Development and pre-validation of a testosterone enzyme immunoassay (EIA) for predicting the sex ratio of immature loggerhead sea turtles (Caretta caretta) recovered along the western coast of the central Adriatic Sea

Plasma testosterone levels were evaluated, by validating and using a commercially available testosterone enzyme immunoassay (EIA) as an indicator of the sex of immature loggerhead turtles (Caretta caretta) recovered along the western coast of the central Adriatic Sea between November 2011 and February 2012. Testosterone levels were measured in blood samples collected from 28 immature turtles kept in short-term maintenance at the Fondazione Cetacea Rescue Centre (Italy). Overall, plasma testosterone ranged between 229.3 and 2628.6 pg ml^?1, suggesting that the EIA procedure is effective for determining androgen titers in immature loggerhead sea turtles. Analysis of the obtained data indicates an unbiased sex ratio supporting previous studies of juvenile loggerhead turtle sex ratios in the Mediterranean Sea. The present work can be considered a starting point for augmenting knowledge on the dynamics of juvenile loggerhead aggregations increasingly found in the northern-central Adriatic Sea and for promoting local management for conservation actions.     

Quantitative assessment of post‐disaster housing recovery: a case study of Punta Gorda,Florida, after Hurricane Charley

Quantitative assessment of post‐disaster housing recovery is critical to enhancing understanding of the process and improving the decisions that shape it. Nevertheless, few comprehensive empirical evaluations of post‐disaster housing recovery have been conducted, and no standard measurement methods exist. This paper presents a quantitative assessment of housing recovery in Punta Gorda, Florida, United States, following Hurricane Charley of August 2004, including an overview of the phases of housing recovery, progression of recovery over time, alternative trajectories of recovery, differential recovery, incorporation of mitigation, and effect on property sales. The assessment is grounded in a conceptual framework that considers the recovery of both people and place, and that emphasises recovery as a process, not as an endpoint. Several data sources are integrated into the assessment—including building permits, remotely sensed imagery, and property appraiser data—and their strengths and limitations are discussed with a view to developing a standardised method for measuring and monitoring housing recovery.     

Effects of flow regimes altered by dams on survival, population declines, and range-wide losses of California river-breeding frogs

Widespread alteration of natural hydrologic patterns by large dams combined with peak demands for power and water delivery during summer months have resulted in frequent aseasonal flow pulses in rivers of western North America. Native species in these ecosystems have evolved with predictable annual flood-drought cycles; thus, their likelihood of persistence may decrease in response to disruption of the seasonal synchrony between stable low-flow conditions and reproduction. We evaluated whether altered flow regimes affected 2 native frogs in California and Oregon (U.S.A.) at 4 spatial and temporal extents. We examined changes in species distribution over approximately 50 years, current population density in 11 regulated and 16 unregulated rivers, temporal trends in abundance among populations occupying rivers with different hydrologic histories, and within-year patterns of survival relative to seasonal hydrology. The foothill yellow-legged frog (Rana boylii), which breeds only in flowing water, is more likely to be absent downstream of large dams than in free-flowing rivers, and breeding populations are on average 5 times smaller in regulated rivers than in unregulated rivers. Time series data (range = 8 - 19 years) from 5 populations of yellow-legged frogs and 2 populations of California red-legged frogs (R. draytonii) across a gradient of natural to highly artificial timing and magnitude of flooding indicate that variability of flows in spring and summer is strongly correlated with high mortality of early life stages and subsequent decreases in densities of adult females. Flow management that better mimics natural flow timing is likely to promote persistence of these species and others with similar phenology.     

Geochemical caper fingerprints as a tool for geographical origin identification

The identification of geographical origin of food products is important for both consumers and producers to ensure quality and avoid label falsifications. The caper plant (Capparis spinosa L., Brassicales Capparidaceae), a xerophytic shrub common in the Mediterranean area, produces buds and fruits that are commercialized in brine at high price. Those grown in Italy in the Aeolian Islands are renowned for their high quality. This study is aimed to establish a correlation between the geological and geochemical features of soil and the chemical composition of caper buds grown in two Aeolian Islands, Lipari and Salina. Major and trace elements were investigated by X-ray fluorescence and inductively coupled plasma-mass spectrometry in soil and caper samples from three localities in Lipari and Salina, and data from the three sites were compared by a nonparametric test, a correlation test and multivariate statistics (principal component analysis). The results allowed to discriminate soils according to geolithological characteristics of each area and detect a statistically significant correspondence between soil and caper samples for the elements Co, Fe, Mg and Rb, identifying thus possible geochemical caper fingerprints of origin. These results may also be useful to protect the high quality of Aeolian caper products by a suitable “Made in Italy” trademark and avoid falsifications and frauds.     

Influence of oceanic factors on long-distance movements of loggerhead sea turtles displaced in the southwest Indian Ocean

The routes of five satellite-tracked loggerhead turtles (Caretta caretta), subjected to an experimental translocation away from their usual migratory routes, have been analysed in relation to the concurrent oceanographic conditions. Remote sensing data on sea surface temperature and height anomalies, as well as trajectories of surface drifters were used, to get simultaneous information on the currents encountered by the turtles during their long-range oceanic movements. Turtles mostly turned out to move in the same direction as the main currents, and their routes were often influenced by circulation features they encountered. A comparison between turtle ground speeds with that of drifters shows that in several instances, the turtles did not drift passively with the currents but contributed actively to the overall movement. Two turtles embarked on an oceanic crossing, probably induced by seasonal changes in surface temperatures, a crossing that was largely determined by the main currents existing in the area.     

Conservation state of two paintings in the Santa Margherita cliff cave: role of the environment and of the microbial community

Environmental Science and Pollution Research - The conservation of ancient paintings sited in humid environments is an actual challenge for restorers, because it needs the knowledge of the...     

The molecular composition of humic substances extracted from green composts and their potential for soil remediation

Humic substances play empirically several essential functions in biogeochemical cycles such as storage of carbon, pollutants, nutrients and water, yet the underlying mechanisms remain poorly known because their precise molecular structure is largely unknown so far. Here, we extracted humic substances from biomass waste of bell pepper, fennel, artichoke, coffee ground, coffee husks, and nursery residues. We analyzed humic extracts by ultra-high resolution Orbitrap Fusion Lumos Tribrid 1 M mass spectrometry, using both positive photoionization and negative electrospray ionization modes, and by ¹³C cross polarization/magic angle spinning nuclear magnetic resonance spectroscopy. We identified 5000–7000 unique organic compounds in humic substances by integrating photoionization with electrospray ionization. The chemical distribution of all components was depicted by nuclear magnetic resonance. Humic substances from green composts are composed by a wide variety of hydrophilic and hydrophobic moieties, thus providing the required biosurfactant properties for effective soil washing capacities, with carboxyl-rich alicyclic molecules, fatty acids, and phenolic acids as major constituents. Overall, our findings provide a major insight in the molecular structure of humic substances, thus opening research on mechanisms ruling the origin, fate and behavior of humic substances.

     

Polycyclic aromatic hydrocarbons in Mullus surmuletus from the Catania Gulf (Sicily,Italy): distribution and potential health risks

Environmental Science and Pollution Research - Different specimens of Mullus surmuletus from the Catania Gulf (Sicily) were sampled and analysed for the quantification of 16 priority polycyclic...     

Results of the monitoring program of pesticide residues in organic food of plant origin in Lombardy (Italy)

Organic agriculture, with its restrictions on the use of synthetic chemical inputs, seems to offer a low-residue alternative to conventional methods. In Europe, the Council Regulation n. 2092/91/EEC regulates the production and trade of organic products and foodstuffs; national and regional legislation in Italy gives specific guidance on the surveillance of organic agriculture. However, monitoring of specific chemical residues in organic foodstuffs is part of the regular controls on food, aiming to safeguard consumer's health. Monitoring programs are coordinated at the national level by the Ministry of Health and at local level by Regional authorities. In Lombardy, in accordance with the provisions of the General Directorate of Health of the Region and under the supervision of the 15 Local Health Units, a monitoring program of pesticide residues in food of plant origin is undertaken every year. The International Centre for Pesticides and Health Risk Prevention (ICPS), on behalf of the General Directorate of Health of the Region of Lombardy, has been collecting and elaborating the data resulting from the analysis of food samples, carried out by the local laboratories. During the period 2002-2005, a total of 3508 samples food of plant origin were analyzed for pesticide residues, among which were 266 samples of organic farming products. Commodities were classified into groups (citrus fruits, legumes, vegetables, potatoes, processed products, cereals, and fruit other than citrus) and the outcomes of the analyses were reported by year, origin of the sample and presence/absence of pesticide residues. Results showed that the vast majority of organic farming products were in conformity with the relevant legislation and did not contain detectable pesticide residues. A limited amount of samples had residues at concentration below the Maximum Residue Limit (MRL). Only in one sample the residue level was above the MRL, however it did not pose a concern for public health, as demonstrated by the outcomes of dietary risk assessment. Organic fruits and vegetables can be expected to contain fewer agrochemical residues than conventionally grown alternatives. There is a widespread belief that organic agriculture products are safer and healthier than conventional foods. It is difficult to come to conclusions, but what should be made clear to the consumer is that "organic" does not automatically equal "safe". In the absence of adequate comparative data, additional studies in this area of research are required.