Automated interpulse-duration assessment (AIDA): a new technique for detecting disturbances in cardiac activity in selected macroinvertebrates

An Automated Interpulse Duration Assessment system (AIDA) is described which permits detection of irregularities in cardiac rhythms in selected invertebrates. The sensitivity of AIDA was demonstrated by its ability to detect handling stress in mussels (Mytilus edulis) that was not evident when measuring heart rate alone. Changes in cardiac activity patterns of crabs (Carcinus maenas) held in the laboratory for up to 10 wk was also examined using the new technique. The frequency distribution of interpulse duration changed significantly as the nutritional state changed. Potential applications of the AIDA system are discussed.     

景观规划导向的绿色基础设施研究进展——基于“格局—过程—服务—可持续性”研究范式

中国目前处于新型城镇化与生态文明建设的重要时期,引入绿色基础设施（Green Infrastructure,GI）规划框架对协调城市发展与生态保护具有重要意义。基于景观生态学中“格局—过程—服务—可持续性”研究范式,对近年来中国内外GI规划进行针对性综述,利用该范式对相关研究的理论来源与进展进行梳理。研究表明：GI格局研究中,景观格局指数是应用最为广泛的方法。针对于景观连接度,形态学空间格局分析等结构连接度评价方法具有计算简单、适用性广的优势,但缺乏明确的生态学含义;图论等功能连接度则针对特定的生态过程,是GI格局与生态过程的重要联系,将是下一步研究重点。GI生态过程研究借鉴生态网络的理论与方法,其规划步骤可分为核心区选取、阻力面构建、潜在廊道识别三部分。GI生态系统服务研究中,生物物理量模型与经济计量模型广泛应用于调节、供给与支持服务评价中,而文化服务评价则将问卷调查作为基础数据获取的重要途径。另外,生态系统服务需求研究涉及到生态系统服务的实际需求与使用偏好,相关定量评价方法以及成果的实际应用有待进一步探索。GI景观可持续性研究中,在明晰GI提供的生态系统服务总量的基础上,服务间权衡与协同效应已成为GI规划中的重要考量,而情景规划可以有效模拟GI生态系统服务的动态变化。最后,GI规划应将提升景观可持续性作为规划目标,据此研究提出了重视格局与过程相互影响机制的基础研究、加深GI生态系统服务需求端研究、明晰生态系统服务间协同与权衡效应、将GI与实际规划结合四项研究展望。     

Fate of citalopram during water treatment with O3, ClO2, UV and Fenton oxidation

In the present study we investigate the fate of citalopram (CIT) at neutral pH using advanced water treatment technologies that include O₃, ClO₂ oxidation, UV irradiation and Fenton oxidation. The ozonation resulted in 80% reduction after 30 min treatment. Oxidation with ClO₂ removed >90% CIT at a dosage of 0.1 mg L⁻¹. During UV irradiation 85% reduction was achieved after 5 min, while Fenton with addition of 14 mg L⁻¹ (Fe²⁺) resulted in 90% reduction of CIT. During these treatment experiments transformation products (TPs) were formed from CIT, where five compounds were identified by using high resolution and tandem mass spectrometry. Among these desmethyl-citalopram and citalopram N-oxide have been previously identified as human metabolites, while three are novel and published here for the first time. The three TPs are a hydroxylated dimethylamino-side chain derivative, a butyrolactone derivative and a defluorinated derivative of CIT.     

Is sustainable resource utilisation a relevant concept in Avanersuaq? The walrus case

This article addresses the role of Atlantic walrus (Odobenus rosmarus rosmarus) in present-day Avanersuaq from anthropological and biological perspectives, and asks whether or not sustainable resource utilisation is a useful concept in northwest Greenland. We describe the relations that unfold around walrus and walrus hunting, in the communities living adjacent to the North Water polynya on the eastern side of Smith Sound. We examine the interplay of walrus population abundance, hunting practices, uses, and formal (governmental) and informal (traditional) ways of regulating the hunt, and we analyse how walruses acquire multiple values as they circulate in different networks. Sustainable resource utilisation, we conclude, is a concept that is relevant in Avanersuaq and beyond, because it works as a biological standard, and hence organises laws, norms, and practices of formal management. Simultaneously, the term is problematic, because it ignores manifold levels of human and societal values connected to walrus.     

Prey-predator dynamics in rotifers: density-dependent consequences of spatial heterogeneity due to surface attachment

Classical models of prey-predator interactions assume that per capita prey consumption is dependent on prey density alone and that prey consumption (functional response) and consumer proliferation (numerical response) operate on the same timescales and without time lags. Several modifications have been proposed for resolving this timescale discrepancy, including variants where the functional response depends on both prey and predator densities. A microcosm system with the rotifer Brachionus 'Nevada' feeding on the prasinophyte Tetraselmis sp. showed significant (P < 0.0005) increases in steady-state biomasses of both prey and predators with increasing carrying capacity (represented by total phosphorus of the growth medium), which is inconsistent with predictions based on the traditional prey-only-dependent functional response. We provide data indicating that surfaces where the predator can attach provide a high-quality habitat for rotifers, which can result in a predator-dependent functional response. We also show that partitioning between the attached and free-swimming habitats was fast compared to the timescale of the numerical response. When attached to surfaces, rotifers maximized net energy gain by avoiding the high cost of swimming and by increased food capture due to reduced viscous drag. A mathematical model with prey-dependent functional response and wall-attached and free-swimming fractions of the population describes our data adequately. We discuss the implications of this finding for extrapolating microcosm experiments to systems with other surface-to-volume ratios, and to what extent our findings may apply to other popular model organisms for prey-predator interaction.     

Non-consumptive effects of predator presence on copepod reproduction: insights from a mesocosm experiment

Reproduction in planktonic animals depends on numerous biotic and abiotic factors. One of them is predation pressure, which can have both direct consumptive effects on population density and sex ratio, and non-consumptive effects, for example on mating and migration behaviour. In copepods, predator vulnerability depends on their sex, motility pattern and mating behaviour. Therefore, copepods can be affected at multiple stages during the mating process. We investigated the reproductive dynamics of the estuarine copepod Eurytemora affinis in the presence and absence of its predator the mysid Neomysis integer in a mesocosm experiment. We found that the proportion of ovigerous females decreased in the presence of predators. This shift was not caused by differential predation as the absolute number of females was unaffected by mysid presence. Presence of predators reduced the ratio of males to non-ovigerous females, but not by predation of males. Our combined results suggest that the shift from ovigerous to non-ovigerous females under the presence of predators was caused by either actively delayed egg production or by shedding of egg sacs. Nauplii production was initially suppressed in the predation treatment, but increased towards the end of the experiment. The proportion of fertilized females was similar in both treatments, but constantly fell behind model predictions using a random mating model. Our results highlight the importance of non-consumptive effects of predators on copepod reproduction and hence on population dynamics.     

De Novo terpenoid biosynthesis by the dendronotid nudibranch Melibe leonina

Summary. Stable isotope feeding studies using [1,2-¹³C₂]-sodium acetate have demonstrated that 2,6-dimethyl-5-heptenal (1), a putative defensive allomone, is made by the Dendronotid nudibranch Melibe leonina via de novo biosynthesis.     

Algal plating as a tool for investigating allelopathy among marine microalgae

Chemical interactions among marine microalgae were studied in cultures of pennate diatoms on agar plates. Nine marine and freshwater pennate diatoms were surveyed as potential bioassay organisms; Cylindrotheca fusiformis was most favourable for assays because of its rapid and even growth on agar. Diatom and bacterial bioassays were used to screen cell and filtrate extracts of 14 microalgal cultures. A number of these algal species, which were grown axenically, produced extractable, intracellular and/or extracellular substances that inhibited the growth of C. fusiformis. Our results suggest that the culturing of pennate diatoms on a solid medium can provide a simple bioassay for screening algal extracts which potentially contain growth inhibitors involved in microalgal allelopathy.     

Phosphorus losses from agricultural areas in river basins: effects and uncertainties of targeted mitigation measures

In this paper we show the quantitative and relative importance of phosphorus (P) losses from agricultural areas within European river basins and demonstrate the importance of P pathways, linking agricultural source areas to surface water at different scales. Agricultural P losses are increasingly important for the P concentration in most European rivers, lakes, and estuaries, even though the quantity of P lost from agricultural areas in European catchments varies at least one order of magnitude (<0.2 kg P ha(-1) to >2.1 kg P ha(-1)). We focus on the importance of P for the implementation of the EU Water Framework Directive and discuss the benefits, uncertainties, and side effects of the different targeted mitigation measures that can be adopted to combat P losses from agricultural areas in river basins. Experimental evidence of the effects of some of the main targeted mitigation measures hitherto implemented is demonstrated, including: (i) soil tillage changes, (ii) treatment of soils near ditches and streams with iron to reduce P transport from source areas to surface waters, (iii) establishment of buffer zones for retaining P from surface runoff, (iv) restoration of river-floodplain systems to allow natural inundation of riparian areas and deposition of P, and (v) inundation of riparian areas with tile drainage water for P retention. Furthermore, we show how river basin managers can map and analyze the extent and importance of P risk areas, exemplified by four catchments differing in size in Norway, Denmark, and the Netherlands. Finally, we discuss the factors and mechanisms that may delay and/or counteract the responses of mitigation measures for combating P losses from agricultural areas when monitored at the catchment scale.