Extinction debt of forest plants persists for more than a century following habitat fragmentation

Following habitat fragmentation individual habitat patches may lose species over time as they pay off their "extinction debt." Species with relatively low rates of population extinction and colonization ("slow" species) may maintain extinction debts for particularly prolonged periods, but few data are available to test this prediction. We analyzed two unusually detailed data sets on forest plant distributions and land-use history from Lincolnshire, United Kingdom, and Vlaams-Brabant, Belgium, to test for an extinction debt in relation to species-specific extinction and colonization rates. Logistic regression models predicting the presence-absence of 36 plant species were first parameterized using data from Lincolnshire, where forest cover has been relatively low (approximately 5-8%) for the past 1000 years. Consistent with extinction debt theory, for relatively slow species (but not fast species) these models systematically underpredicted levels of patch occupancy in Vlaams-Brabant, where forest cover was reduced from approximately 25% to <10% between 1775 and 1900 (it is presently 6.5%). As a consequence, the ability of the Lincolnshire models to predict patch occupancy in Vlaams-Brabant was worse for slow than for fast species. Thus, more than a century after forest fragmentation reached its current level an extinction debt persists for species with low rates of population turnover.     

Interplay between Senecio jacobaea and plant, soil, and aboveground insect community composition

To elucidate the factors that affect the performance of plants in their natural environment, it is essential to study interactions with other neighboring plants, as well as with above- and belowground higher trophic organisms. We used a long-term field experiment to study how local plant community diversity influenced colonization by the biennial composite Senecio jacobaea in its native range in The Netherlands in Europe. We tested the effect of sowing later-succession plant species (0, 4, or 15 species) on plant succession and S. jacobaea performance. Over a period of eight years, the percent cover of S. jacobaea was relatively low in communities sown with 15 or 4 later-succession plant species compared to plots that were not sown, but that were colonized naturally. However, after four years of high abundance, the density of S. jacobaea in unsown plots started to decline, and the size of the individual plants was smaller than in the plots sown with 15 or 4 plant species. In the unsown plots, densities of aboveground leaf-mining, flower-feeding, and stem-boring insects on S. jacobaea plants were lower than on plants in sown plots, and there was a strong positive relationship between plant size and levels of herbivory. In a greenhouse experiment, we grew S. jacobaea in sterilized soil inoculated with soil from the different sowing treatments of the field experiment. Biomass production was lower when S. jacobaea test plants were grown in soil from the unsown plots than in soil from the sown plots (4 or 15 species). Molecular analysis of the fungal and bacterial communities revealed that the composition of fungal communities in unsown plots differed significantly from those in sown plots, suggesting that soil fungi could have been involved in the relative growth reduction of S. jacobaea in the greenhouse bioassay. Our results show that, in its native habitat, the abundance of S. jacobaea depends on the initial composition of the plant community and that, on a scale of almost a decade, its interactions with plant and soil communities and aboveground invertebrates may influence the dynamics of this colonizing species.     

Ecological gradients and relative abundance of native (Mytilus trossulus) and invasive (Mytilus galloprovincialis) blue mussels in the California hybrid zone

Marine communities are experiencing unprecedented rates of species homogenization due to the increasing success of invasive species, but little is known about the mechanisms that allow a species to invade and persist in a new habitat. In central California, native (Mytilus trossulus Gould 1850) and invasive (Mytilus galloprovincialis Lamarck 1819) blue mussels and their hybrids co-exist, providing an opportunity to analyze the mechanisms that determine the distributions of these taxa. Spatial and temporal variation in temperature and salinity and the relative frequencies of these mussel taxa were examined between 2000 and 2004 at four sites in San Francisco Bay and four in Monterey Bay, which were chosen for their different positions along inferred estuarine/oceanic gradients in the hybrid zone. Mussels were genetically identified as the parent species or hybrids by amplifying regions of two species-specific loci: the adhesive byssal thread protein (Glu-5′) and the internal transcribed spacer region of ribosomal DNA (ITS 1). The proportion of M. trossulus at the eight hybrid zone sites correlated negatively with average salinity (R ²=0.60) and positively with maximal temperature (R ²≥0.72), a somewhat unexpected result given what is known about the phylogeography of this species. The proportion of M. galloprovincialis showed the opposite pattern. The proportion of hybrids was correlated neither with habitat temperature nor salinity. Genotypes of mussel populations at an additional 13 sites from Coos Bay, Oregon (latitude 43.35°N) to Long Beach, California (latitude 33.72°N), sampled at various intervals between 2000 and 2004, were also determined. This survey confirmed previous reports that the hybrid zone lies between Monterey and the Cape Mendocino region (latitudes 36.63°N–40.5°N). Within Monterey and San Francisco Bays, however, the temporal comparisons (1990s vs. 2000s) revealed abrupt changes in the proportions of the two parent species and their hybrids on annual and decadal time scales. These changes indicate that the blue mussel populations are in a highly dynamic state. The survey also showed that, regardless of habitat, M. trossulus is consistently of smaller average size than either M. galloprovincialis or hybrids.

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Recovery by riffle macroinvertebrates in a river after a major accidental spillage of chlorpyrifos

The impact of a major spillage of the insecticide 'Dursban' on the riffle macroinvertebrates along 19 km of the River Roding, Essex, is described. Five affected riffles and an upstream control were kick-sampled at approximately 10-week intervals for two years following the spill, and the results are compared with species composition and relative abundance data collected from the same sites during the previous six years. Initial concentrations of the active ingredient, chlorpyrifos, in river water (up to 2.5 mg litre(-1)) exceeded the level lethal to all the aquatic arthropods present by at least 10-fold, and this group of macroinvertebrates was eliminated. Molluscs and annelids, which are relatively tolerant of chlorpyrifos, survived. Since these groups already dominated the lowermost urban reaches, the impact of the spill was greatest further upstream, where reaches with a better water quality previously supported a more diverse and abundant arthropod fauna. Chlorpyrifos residues in water declined below 1 microg litre(-1) within 11 weeks, but sediment within 5 km of the spillage site remained highly contaminated for considerably longer. Of ten arthropod taxa previously common to all sites, chironomid larvae were first to recolonise affected reaches, 13 weeks after the spill. The isopod Asellus aquaticus, was also quick to recover. Although other arthropods had recolonised most sites within 79 weeks, the coleopteran Oulimnius tuberculatus and the epheremeropteran Caenis moesta had failed to return to the lowermost reaches after 108 weeks. Downstream drift was probably the principal factor influencing the pattern of arthropod recolonisation, with the location and aquatic fauna of tributary streams entering the affected reaches being particularly important.     

Prenatal diagnosis of partial tetrasomy 14: a case study

Prenatal specimens were received from a fetus with abnormalities noted on ultrasound. A supernumerary marker chromosome (SMC) was detected: 47,XY,+mar. Fluorescence in situ hybridisation (FISH) further classified this to be partial tetrasomy for chromosome 14. We compare this finding with other cases of SMC (14) and further classify phenotype with karyotype. Copyright © 2002 John Wiley & Sons, Ltd.     

Stochastic mortality theory and the mortality potential: A biophysical model for certain competing risks

The theory of competing risks cannot be based entirely on the analysis of postmortem pathology data. It is necessary also to know the prevalence and residence times of the diseases in question, separately and jointly, in the living population at risk. It is also necessary to have a conceptual model of the transition process from health to disease, or from one disease state to another, within the organism, so that the formal transition probabilities estimated from epidemiological data can be interpreted in biophysical terms as arising from changes in the physico-chemical state of the organism. This paper offers such a model for transition processes arising from fluctuations of physiological state (for example, lapse into diabetic coma or insulin shock as a result of extreme high or low excursions of the blood sugar level). A Gaussian fluctuation process is postulated, and the transition probability, or incidence rate, is calculated as the frequency with which the state variable fluctuates beyond a specified distance, Λ, from the mean state. An explicit solution is given for the limiting, but biologically reasonable, case that such an excursion is a rare event. In this case, the transition probability varies exponentially for linear displacement of Λ or of the dispersion of fluctuations, σ. If Λ decreases, and/or σ increases, as a linear function of age, this model yields an exponential (Gompertzian) relation of disease incidence to age. Generalization to more than one variable is accomplished by introducing the concept of a mortality potential surface, in which the disease transitions are geometrized as saddle points, or “passes”, on the surface.     

Genetic and Demographic Threats to the Black Rhinoceros Population in the Ngorongoro Crater

A resident population of 13 black rhinoceros ( Diceros bicornis ) persist in Ngorongoro Crater, Tanzania. The effective population size ( N _e ) may be as few as 5 animals. Projected growth for this population suggests that the effective population size will remain small for the near future, threatening this Iocal population with extinction due to the stochastic factors associated with small population size. A summary of historic and recent demographic data for this population reveals a population crash during the period of heavy poaching that affected this species throughout its range. Although poaching of this species has been brought under control the population remains small. These data and models of projected population growth argue for consideration of more-intensive management within the framework of the small population paradigm. This case is an example of applied conservation resulting from this paradigm used in conjunction with rather than competing with the declining population paradigm. We identify additional monitoring, particularly of density-dependent behaviors, that will be necessary for designing a successful management program. Finally, the use of molecular markers for developing an accurate pedigree for this population is suggested in order to maintain a genetically healthy population. These strategies have broad applicability to black rhinoceros conservation throughout Africa.     

Contaminants evaluation of Marais des Cygnes National Wildlife Refuge in Kansas and Missouri,USA

At the new Marais des Cygnes National Wildlife Refuge in Linn County, Kansas, and Bates County, Missouri, USA, we evaluated long-lived contaminants before acquisition of the land for the refuge. We sampled sediments at 16 locations and fish at seven locations. The samples were analyzed for metals and for chlorinated hydrocarbon compounds. Selected sediment samples also were analyzed for aliphatic hydrocarbons. Arsenic concentrations in sediment samples from six locations were elevated compared to US norms, but arsenic was not detected in any fish composite. Mercury concentrations in largemouth bass from two locations were comparable to the 85th percentile concentrations in nationwide fish collections. Most sediment concentrations of other metals were unlikely to have detrimental effects on biota. No chlorinated hydrocarbons were detected in any sediment sample. Chlordane compound concentrations in fish composites from two sites at the eastern end of the sampling area were 0.127 and 0.228 μg/g wet weight, respectively, which are high enough to cause concern. Most aliphatic hydrocarbons detected were found at low concentrations and probably were natural in origin. We concluded that there are no serious contaminants concerns within the project area, but past use of arsenical pesticides may mean a legacy of elevated soil arsenic levels in parts of the area and some use of banned pesticides such as chlordane and DDT likely is still occurring near the refuge.