排序方式: 共有9条查询结果,搜索用时 15 毫秒
1
1.
2.
3.
C. Metaxotou A. Antsaklis P. Panagiotopoulou M. Benetou A. Mavrou N. Matsaniotis 《黑龙江环境通报》1987,7(7):461-469
Several methods for fetal chromosome analysis using chorionic biopsy samples were compared. A modified direct method for culturing villi was considered to be the method of choice and details are presented of 186 pregnancies tested prenatally. The success rate in obtaining a fetal karyotype with the direct method was 93 per cent. The fetal loss rate in the prenatal series was 4.3 per cent and congenital abnormalities in the babies already born did not differ from the expected incidence. 相似文献
4.
5.
6.
Dr. C. Metaxotou A. Antsaklis P. Panagiotopoulou Ch. Tsenghi M. Benetou A. Mavrou N. Matsaniotis 《黑龙江环境通报》1983,3(2):173-175
The fetal karyotype was determined in 42 out of 45 cases from fetal blood obtained by fetoscopy for prenatal diagnosis of β-thalassemia. The procedure described is quick and reliable and it is recommended for women over 35 years of age undergoing prenatal diagnosis for haemoglobinopathies. 相似文献
7.
8.
A. Kolialexi C. Vrettou J. Traeger-Synodinos R. Burgemeister N. Papantoniou E. Kanavakis A. Antsaklis A. Mavrou 《黑龙江环境通报》2007,27(13):1228-1232
Single nucleated red blood cells (NRBCs) isolated from maternal circulation were used for prenatal diagnosis of β-thalassaemia. The study included 22 pregnant women in the first trimester, 6 carriers at risk for β-thalassaemia and 16 noncarriers. Methodology involved enrichment of NRBCs by magnetic cell sorting (MACS) and microdissection of single NRBCs with a laser micromanipulation system. Single-cell genotyping based on nested real-time PCR for genotyping β-globin gene mutations was performed followed by a multiplexed minifingerprinting to confirm the origin of the isolated cells and possible contamination. Two polymorphic markers (D13S314 and GABRB3) facilitated the identification of fetal NRBCs through comparison of allele sizes found in the respective parents. In this study, 224 single NRBCs were detached and transferred into individual PCR tubes. Allele amplification in at least one microsatellite marker was achieved in 128/224 cells. Minifingerprinting analysis showed that 22 cells were fetal, 26 maternal and 80 were noninformative due to ADO or homozygosity. In 6 NRBCs the β-globin gene was amplified and in 2, coming from the same pregnancy, only the paternal mutation was detected. The low PCR success when genotyping isolated NRBCs was possibly due to the poor quality of fetal NRBCs and the relatively large size of the β-globin gene product. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献
9.
Tobias J. Legler Zhong Liu Ariadni Mavrou Kirstin Finning Ilona Hromadnikova Silvia Galbiati Cathy Meaney Maj A. Hultén Francesco Crea Martin L. Olsson Deborah G. Maddocks Dorothy Huang Sylvia Armstrong Fisher Markus Sprenger-Haussels Aicha Ait Soussan C. Ellen van der Schoot 《黑龙江环境通报》2007,27(9):824-829
Objective Cell free foetal DNA (cff DNA) extracted from maternal plasma is now recognized as a potential source for prenatal diagnosis but the methodology is currently not well standardized. To evaluate different manual and automated DNA extraction methods with a view to developing standards, an International Workshop was performed. Methods Three plasma pools from RhD-negative pregnant women, a DNA standard, real-time-PCR protocol, primers and probes for RHD were sent to 12 laboratories and also to one company (Qiagen, Hilden, Germany). In pre-tests, pool 3 showed a low cff DNA concentration, pool 1 showed a higher concentration and pool 2 an intermediate concentration. Results The QIAamp DSP Virus Kit, the High Pure PCR Template Preparation Kit, an in-house protocol using the QIAamp DNA Blood Mini Kit, the CST genomic DNA purification kit, the Magna Pure LC, the MDx, the M48, the EZ1 and an in-house protocol using magnetic beads for manual and automated extraction were the methods that were able to reliably detect foetal RHD. The best results were obtained with the QIAamp DSP Virus Kit. The QIAamp DNA Blood Mini Kit showed very comparable results in laboratories that followed the manufacturer's protocol and started with ≥ 500 µL plasma. One participant using the QIAamp DNA Blood Midi Kit failed to detect reliably RHD in pool 3. Conclusions This workshop initiated a standardization process for extraction of cff DNA in maternal plasma. The highest yield was obtained by the QIAamp DSP Virus Kit, a result that will be evaluated in more detail in future studies. Copyright © 2007 John Wiley & Sons, Ltd. 相似文献
1