Microbial degradation of pyridine using Pseudomonas sp. and isolation of plasmid responsible for degradation

Pseudomonas (PI2) capable of degrading pyridine was isolated from the mixed population of the activated sludge unit which was being used for treating complex effluents, the strain was characterized. Aerobic degradation of pyridine was studied with the isolated strain and the growth parameters were evaluated. Pyridine degradation was further conformed by chromatography (HPLC) analysis. The process parameters like biomass growth and dissolved oxygen consumption were monitored during pyridine degradation. In order to conform with the plasmid capability to degrade pyridine, the requisite plasmid was isolated and transferred to DH 5alpha Escherichia coli. The subsequent biodegradation studies revealed the ability of the transformed plasmid capability to degrade the pyridine.     

Kinetic parameters for the removal of lead and chromium from wastewater using activated carbon developed from fertilizer waste material

The waste slurry generated in fertilizer plants in India has been converted into a cheap carbonaceous adsorbent material. The prepared adsorbent has been characterised and used for the removal of lead and chromium metals. The kinetics of adsorption and the extent of adsorption at equilibrium are dependent on the physical and chemical characteristics of the adsorbate, adsorbent and experimental system. Results of laboratory scale studies conducted to delineate the effect of such parameters on the kinetics of adsorption of metal ions are reported. Parameters evaluated include: hydronium ion concentration, temperature, initial adsorbate concentration, size of adsorbent, and amount of adsorbent. On the basis of these studies the various physical parameters such as effective diffusion coefficient, activation energies and entropy of activation are evaluated, as these provide some information regarding the mechanistic aspects. Mass transfer coefficient values suggest a rapid transport of the adsorbate from bulk to solid phase.     

Effectiveness of the designed safety education programme modules by their implementation in selected industries

Various and diverse industrial safety areas were identified and grouped as under: general areas of safety, Special areas of safety and industry specific areas of safety.

General areas of safety are common to all industries and special areas of safety are relevant only if the selected industry has such nature of work or hazard and whereas the industry specific areas of safety refers to process safety of the industry. Three separate modules were prepared along with contents.

For conduction of the programme, the selected industry must be studied and the designed safety education programme modules have to be adapted by leaving irrelevant areas in special and industry specific areas of the designed safety modules. The implementation of safety education programme was carried out at Bharath Heavy Electricals Ltd, Trichy and Southern Railway workshop, Trichy, by adapting the designed modules to suit these industries as mentioned above.

Effectiveness is measured by the increase in safety awareness of the selected industrial workers (sample), who had already undergone such programmes with short falls conducted within the industry itself. Research Tool was prepared based on the contents of the adapted safety education modules and pre-test and post-test were conducted, data collected and analysed.

The results were statistically significant as confirmed by t-test analyses.     

Corrosion and polarization behavior of carbon steel in MEA-based CO2 capture process

This work reveals levels of corrosion rate and polarization behavior of carbon steel immersed in aqueous solutions of monoethanolamine (MEA) used in the absorption-based carbon dioxide (CO₂) capture process for greenhouse gas reduction from industrial flue gas streams. Such information was obtained from electrochemical-based corrosion experiments under a wide range of the CO₂ capture process conditions. The corrosion of carbon steel was evaluated in respect to process parameters including partial pressure of oxygen (O₂), CO₂ loading in solution, solution velocity, solution temperature, MEA concentration and metal surface condition. Results show that the aqueous MEA solution containing CO₂ provides a favorable condition for the corrosion of carbon steel to proceed. Corrosion rate is increased by all tested process parameters. These parametric effects were explained by the electrochemical kinetic data obtained from polarization curves and by the thermodynamic data obtained from Pourbaix diagram.     

Characteristics of worker accidents on NYSDOT construction projects

INTRODUCTION: This paper aims at providing cost-effective safety measures to protect construction workers in highway work zones, based on real data. Two types of accidents that occur in work zones were: (a) construction work area accidents, and (b) traffic accidents involving construction worker(s). METHODOLOGY/RESULTS: A detailed analysis of work zone accidents involving 36 fatalities and 3,055 severe injuries to construction workers on New York State Department of Transportation (NYSDOT) construction projects from 1990 to 2001 established that five accident types: (a) Struck/Pinned by Large Equipment, (b) Trip or Fall (elevated), (c) Contact w/Electrical or Gas Utility, (d) Struck-by Moving/Falling Load, and (e) Crane/Lift Device Failure accounted for nearly 96% of the fatal accidents, nearly 63% of the hospital-level injury accidents, and nearly 91% of the total costs. These construction work area accidents had a total cost of $133.8 million. Traffic accidents that involve contractors' employees were also examined. Statistical analyses of the traffic accidents established that five traffic accident types: (a) Work Space Intrusion, (b) Worker Struck-by Vehicle Inside Work Space, (c) Flagger Struck-by Vehicle, (d) Worker Struck-by Vehicle Entering/Exiting Work Space, and (e) Construction Equipment Struck-by Vehicle Inside Work Space accounted for nearly 86% of the fatal, nearly 70% of the hospital-level injury and minor injury traffic accidents, and $45.4 million (79.4%) of the total traffic accident costs. CONCLUSIONS: The results of this paper provide real statistics on construction worker related accidents reported on construction work zones. Potential preventions based on real statistics have also been suggested. IMPACT ON INDUSTRY: The ranking of accident types, both within the work area as well as in traffic, will guide the heavy highway contractor and owner agencies in identifying the most cost effective safety preventions.     

Total and methyl mercury in the water,sediment, and fishes of Vembanad,a tropical backwater system in India

Mercury contamination in the water bodies of developing countries is a serious concern due to its toxicity, persistence, and bioaccumulation. Vembanad, a tropical backwater lake situated at the southwest coast of India, is the largest Ramsar site in southern India. The lake supports thousands of people directly and indirectly through its resources and ecosystem services. It is highly polluted with toxic pollutants such as heavy metals, as it receives effluent discharges from Kerala’s major industrial zone. In the present study, water, pore water, sediment, and fish samples collected from Vembanad Lake were analysed for total mercury (THg) and methyl mercury (MHg) contents. The maximum concentrations of THg and MHg in surface water samples were31.8 and 0.21 ng/L, respectively, and those in bottom water samples were 206 and 1.22 ng/L, respectively. Maximum concentration of THg in surface sediment was observed during monsoon season (2850 ng/g) followed by that in the pre-monsoon season (2730 ng/g) and the post-monsoon season (2140 ng/g). The highest sediment concentration of MHg (202.02 ng/g) was obtained during monsoon season. The spatial variation in the mercury contamination clearly indicates that the industrial discharge into the Periyar River is a major reason for pollution in the lake. The mercury pollution was found to be much higher in Vembanad Lake than in other wetlands in India. The bioaccumulation was high in carnivorous fishes, followed by benthic carnivores. The THg limit in fish for human consumption (0.5 mg/kg dry wt.) was exceeded for all fish species, except for Glossogobius guiris and Synaptura orientalis. The concentration of THg was five times higher in Megalops cyprinoides and four times higher in Gazza minuta. Significant variation was observed among species with different habits and habitats. Overall, risk assessment factors showed that the mercury levels in the edible fishes of Vembanad Lake can pose serious health impacts to the human population.     

Outdoor 220Rn, 222Rn and terrestrial gamma radiation levels: investigation study in the thorium rich Fen Complex, Norway

The present study was done in the Fen Complex, a Norwegian area rich in naturally occurring radionuclides, especially in thorium ((232)Th). Measurement of radioactivity levels was conducted at the decommissioned iron (Fe) and niobium (Nb) mining sites (TENORM) as well as at the undisturbed wooded sites (NORM), all open for free public access. The soil activity concentrations of (232)Th (3280-8395 Bq kg(-1)) were significantly higher than the world and the Norwegian average values and exceeded the Norwegian screening level (1000 Bq kg(-1)) for radioactive waste, while radium ((226)Ra) was present at slightly elevated levels (89-171 Bq kg(-1)). Terrestrial gamma dose rates were also elevated, ranging 2.6-4.4 μGy h(-1). Based on long-term surveys, the air concentrations of thoron ((220)Rn) and radon ((222)Rn) reached 1786 and 82 Bq m(-3), respectively. Seasonal variation in the outdoor gamma dose rates and Rn concentrations was confirmed. Correlation analyses showed a linear relationship between air radiation levels and the abundance of (232)Th in soil. The annual outdoor effective radiation doses for humans (occupancy 5 h day(-1)) were estimated to be in the range of 3.0-7.7 mSv, comparable or higher than the total average (summarized indoor and outdoor) exposure dose for the Norwegian population (2.9 mSv year(-1)). On the basis of all obtained results, this Norwegian area should be considered as enhanced natural radiation area (ENRA).     

Bio-mediated silver nanoparticle synthesis: mechanism and microbial inactivation

Even though plenty of literature is available on the biosynthesis of metal nanoparticles, there are serious lacunae on the mechanisms of their formation. In the present study, the mechanism of formation of mono-crystalline silver nanoparticles using a fruit extract of the ornamental tree Thevetia peruviana is emphasized, i.e. how the pH of the reaction mixture affected reaction kinetics and size of the nanoparticles. The facilitation of formation of Ag₂O at higher pH resulted in a faster rate of particle formation. The diameter of the bare particles at neutral pH determined by field emission scanning electron microscopy and the hydrodynamic diameter determined by dynamic light scattering were 53 and 104 nm, respectively. The silver nanoparticles exhibited good inactivation of Escherichia coli due to participation of free radicals as evidenced by electron spin resonance spectroscopy.     

Photocatalytic degradation of p-nitrophenol using biologically synthesized ZnO nanoparticles

Environmental Science and Pollution Research - The present work deals with the photocatalytic degradation of p-nitrophenol as it is a United States Environmental Protection Agency-listed priority...     

Viral Interference as a Factor of False-Negative in the Infectious Adenovirus Detection Using Integrated Cell Culture-PCR with a BGM Cell Line

Food and Environmental Virology - This study investigated the influence of viral interference on the detection of enteric viruses using the integrated cell culture (ICC)-PCR with a BGM cell line....