Tolerance of transgenic canola plants (Brassica napus) amended with plant growth-promoting bacteria to flooding stress at a metal-contaminated field site

The growth of transgenic canola (Brassica napus) expressing a gene for the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase was compared to non-transformed canola exposed to flooding and elevated soil Ni concentration, in situ. In addition, the ability of the plant growth-promoting bacterium Pseudomonas putida UW4, which also expresses ACC deaminase, to facilitate the growth of non-transformed and transgenic canola under the above mentioned conditions was examined. Transgenic canola and/or canola treated with P. putida UW4 had greater shoot biomass compared to non-transformed canola under low flood-stress conditions. Under high flood-stress conditions, shoot biomass was reduced and Ni accumulation was increased in all instances relative to low flood-stress conditions. This is the first field study to document the increase in plant tolerance utilizing transgenic plants and plant growth-promoting bacteria exposed to multiple stressors.     

Sampling and Analysis of Quaternary Ammonium Compounds (QACs) Traces in Indoor Atmosphere

Quaternary Ammonium Compounds (QACs) are widely found in disinfectants used in hospitals. Benzalkonium chloride (BAC) and didecyldimethylammonium chloride (DDAC) predominate in the disinfecting formulations. These compounds are strong irritants and can play a role in the induction of Occupational Asthma among the professionals of health and cleaning. In order to evaluate the potential health effect of these quaternary ammonium compounds to hospital employers, the development of an analytical method for their quantification in indoor air was developed. DDAC aerosols are trapped by adsorption on XAD-2 resin SKC tube. The air in hospital buildings was sampled using a constant debit Gillian pump at a flow of 1.0 l/min (+/-5%). Ion Chromatography (IC) was chosen for the analysis of DDAC especially for its high sensitivity and specificity. The Limit of Detection (LOD) by IC for DDAC is 0.56 mug/ml. Therefore the LOD of atmospheric DDAC is 28 microg/m(3) with an air volume of 100 l and a desorption volume of 5 ml. All DDAC air samples were lower than the LOD of the analytical method by IC. Under the standard conditions of use of the disinfecting solutions (Surfanios, Ampholysine Plus and Amphospray 41), the insignificant volatility of DDAC would not seem to be able to contaminate the indoor hospital atmosphere during the disinfection process. However, the DDAC can contaminate working atmospheres if it is put in suspension by aerosolisation.     

Estimating Evapotranspiration and Seepage for a Sinkhole Wetland From Diurnal Surface‐Water Cycles1

Abstract: This study used measured diurnal surface‐water cycles to estimate daily evapotranspiration (ET) and seepage for a seasonally flooded sinkhole wetland. Diurnal surface‐water cycles were classified into five categories based on the relationship between the surface‐water body and the surrounding ground‐water system (i.e., recharge/discharge). Only one class of diurnal cycles was found to be suitable for application of this method. This subset of diurnal cycles was used to estimate ET and seepage and the relative importance of each transfer process to the overall water budget. The method has limited utility for wetlands with erratic hydrologic regimes (e.g., wetlands in urban environments). This is due to violation of the critical assumption that the inflow/outflow rate remains constant throughout the day. For application to surface‐water systems, the method is typically applied with an assumed specific yield of 1.0. This assumption was found to be invalid for application to surface‐water systems with a noncylindrical pond geometry. An overestimation of ET by as much as 60% was found to occur under conditions of low pond stage and high water loss. The results demonstrate the high ET rates that can occur in isolated wetlands due to contrasting roughness and moisture conditions (oasis and clothesline effects). Estimated ET rates ranged from 4.1 to 18.7 mm/day during the growing season. Despite these large ET rates, seepage (recharge) was found to be the dominant water loss mechanism for the wetland.     

Effect of indigenous bacterial activity on arsenic mobilization under anaerobic conditions

Batch biochemical leaching tests were carried out to investigate the mobility of arsenic from a contaminated soil collected from a French gold mining site. The specific objective of this research was to examine the effect of indigenous bacterial activity on arsenic mobilization under anaerobic conditions. In a first step, physical and chemical characterizations were performed to provide data concerning the liquid-solid partitioning and mobility of arsenic and other inorganic constituents. In a second step, batch bioleaching tests were conducted in shaker flasks to determine the effect of indigenous bacterial activity under different anaerobic conditions (i.e., addition of mineral nutrients and carbon sources) on arsenic mobilization. Results indicated that arsenic release during contact with deionized water was limited by its very low solubility in the interstitial solution and by the stability of the different arsenic compounds formed with the amorphous solid phases of the soil (mainly iron (oxy)hydroxides). However, an increased mobilization potential was observed over the long term under anaerobic conditions with indigenous bacterial activity enhanced by the addition of carbon sources.     

Development of a limit state approach for design against gas explosions

The design of topsides against explosions requires the definition of a design over-pressure, however, these values are often treated as deterministic and there is a wide variation within the industry in the treatment and interpretation of the loads.

This paper advocates the adoption of a number of limit state for explosion loading. Events of different magnitudes are differentiated on the basis of frequency and linked to appropriate degree of reliability thus avoiding disproportionate effects from minor events.

The two principal limit states considered are a limit state for all the safety critical systems for relatively high frequency events and a survival condition for low probability events. Parallels are drawn from other branches of engineering where extreme loads have to be designed for.     

Oxidation of SO2 in Clouds at Whiteface Mountain

In-cloud oxidation of SO₂ byH₂O₂ was investigated using a tracertechnique based on SO^2– ₄/Se ratios atWhiteface Mountain, New York during summer months from1990 to 1998. Cloud water samples collected at themountain's summit (1.5 km above mean sea level) andaerosols at a below cloud site (Lodge) located at 0.6km amsl and in cloud interstitial air at the summitwere analyzed for SO^2– ₄ and selectedtrace elements. Gaseous SO₂ andH₂O₂were measured in realtime. Cloud water pH wasgenerally below 5.0 with a mean value of 3.6. Theresults show that significant in cloud oxidation occursin clouds during summer months varying from belowdetection to 62% with on average approximately 24% ofthe cloud water SO^2– ₄ produced from in-situ SO₂ oxidation. During summer the clouds wereoxidant limited for approximately one third of thetime.     

Do ovarian scars persist with age in all Cetaceans: new insight from the short-beaked common dolphin (<Emphasis Type="Italic">Delphinus delphis</Emphasis> Linnaeus<Emphasis Type="Italic">,</Emphasis> 1758)

The determination of reproductive status and the reconstruction of individual reproductive histories are central to many ecological studies. In cetaceans, it has been assumed that ovarian scars accumulate with age and provide a lifetime record of female reproductive history. If ovarian scars persist, the number of scars should increase with age after puberty. To test this, we examined age, reproductive status and ovarian scars from 187 short-beaked common dolphins (Delphinus delphis) from the eastern North Atlantic. The number of Corpus Albicans (CA) present in ovaries did not increase with age after age at sexual maturity (ASM), suggesting that ovarian scars are not persistent and that their number at any one time would be a function of rates of ovulation and healing, the latter being defined here as the resorption or disintegration of CA tissue. Since female mammals stop ovulating when pregnant, inferences about healing rates could be made by using pregnant females. Pregnant females had ca. 40% fewer scars than non-pregnant females. This suggests that most CAs would heal quickly, with a half-life of less than 1 year, although larger scars may persist longer. Therefore, counting CAs would have limited potential for reconstructing individual reproductive lifetime histories in the common dolphin. A reassessment of the use of ovarian scars to reconstruct individual life reproductive histories in cetaceans is suggested.     

Selecting parameters for calibration via sensitivity analysis: An individual-based model of mosquitofish population dynamics

A stochastic individual-based model (IBM) of mosquitofish population dynamics in experimental ponds was constructed in order to increase, virtually, the number of replicates of control populations in an ecotoxicology trial, and thus to increase the statistical power of the experiments. In this context, great importance had to be paid to model calibration as this conditions the use of the model as a reference for statistical comparisons. Accordingly, model calibration required that both mean behaviour and variability behaviour of the model were in accordance with real data. Currently, identifying parameter values from observed data is still an open issue for IBMs, especially when the parameter space is large. Our model included 41 parameters: 30 driving the model expectancy and 11 driving the model variability. Under these conditions, the use of “Latin hypercube” sampling would most probably have “missed” some important combinations of parameter values. Therefore, complete factorial design was preferred. Unfortunately, due to the constraints of the computational capacity, cost-acceptable “complete designs” were limited to no more than nine parameters, the calibration question becoming a parameter selection question. In this study, successive “complete designs” were conducted with different sets of parameters and different parameter values, in order to progressively narrow the parameter space. For each “complete design”, the selection of a maximum of nine parameters and their respective n values was carefully guided by sensitivity analysis. Sensitivity analysis was decisive in selecting parameters that were both influential and likely to have strong interactions. According to this strategy, the model of mosquitofish population dynamics was calibrated on real data from two different years of experiments, and validated on real data from another independent year. This model includes two categories of agents; fish and their living environment. Fish agents have four main processes: growth, survival, puberty and reproduction. The outputs of the model are the length frequency distribution of the population and the 16 scalar variables describing the fish populations. In this study, the length frequency distribution was parameterized by 10 scalars in order to be able to perform calibration. The recently suggested notion of “probabilistic distribution of the distributions” was also applied to our case study, and was shown to be very promising for comparing length frequency distributions (as such).