Re-evaluation of the yield response to phosphorus fertilization based on meta-analyses of long-term field experiments

Phosphorus (P) fertilizer recommendations in most European countries are based on plant-available soil P contents and long-term field experiments. Site-specific conditions are often neglected, resulting in excessive P fertilizer applications. P fertilization experiments including relevant site and soil parameters were evaluated in order to analyze the yield response. The database comprises about 2000 datasets from 30 field experiments from Germany and Austria. Statistical evaluations using a classification and regression tree approach, and multiple linear regression analysis indicate that besides plant-available soil P content, soil texture and soil organic matter content have a large influence on the effectiveness of P fertilization. This study methodology can be a basis for modification and specification of existing P fertilization recommendations and thus contribute to mitigate environmental impacts of P fertilization.     

Seasonal dynamics of freshwater pathogens as measured by microarray at Lake Sapanca,a drinking water source in the north-eastern part of Turkey

Monitoring drinking water quality is an important public health issue. Two objectives from the 4 years, six nations, EU Project μAqua were to develop hierarchically specific probes to detect and quantify pathogens in drinking water using a PCR-free microarray platform and to design a standardised water sampling program from different sources in Europe to obtain sufficient material for downstream analysis. Our phylochip contains barcodes (probes) that specifically identify freshwater pathogens that are human health risks in a taxonomic hierarchical fashion such that if species is present, the entire taxonomic hierarchy (genus, family, order, phylum, kingdom) leading to it must also be present, which avoids false positives. Molecular tools are more rapid, accurate and reliable than traditional methods, which means faster mitigation strategies with less harm to humans and the community. We present microarray results for the presence of freshwater pathogens from a Turkish lake used drinking water and inferred cyanobacterial cell equivalents from samples concentrated from 40 into 1 L in 45 min using hollow fibre filters. In two companion studies from the same samples, cyanobacterial toxins were analysed using chemical methods and those dates with highest toxin values also had highest cell equivalents as inferred from this microarray study.     

Typologies of collaborative governance for scaling nature-based solutions in two strategic South African river systems

Scaled up planning and implementation of nature-based solutions requires better understanding of broad characteristics (typologies) of the current governance and financing landscape, collaborative approaches amidst local complexities, and factors of scalability. An inventory was compiled of water-related ecological infrastructure intervention projects in two river systems in South Africa, incorporating actor, environmental, social, and financial dimensions and benefits. Qualitative participatory analysis revealed eight typologies. Post-hoc classification analysis determined similarities and/or unique characteristics of seven quantitative typologies. Key characterising factors included the complexity/size of financial flows, complexity of partnership/governance arrangements, mandates/goals of actors, type of ecological infrastructure, trade-offs in investment in ecological/built infrastructure, and the model used for social benefits. Identified scalable typologies offer structures suited to increased investment, with other typologies offering specialised local value. A range of ecological infrastructure intervention typologies with differing biophysical and socioeconomic outcomes provide choices for investors with specific goals, and benefits to landscape actors.Supplementary InformationThe online version contains supplementary material available at 10.1007/s13280-021-01531-z.     

A Food Handler-Associated, Foodborne Norovirus GII.4 Sydney 2012-Outbreak Following a Wedding Dinner, Austria, October 2012

On October 12, 2012, the provincial public health directorate of Salzburg reported a suspected norovirus (NV) outbreak among guests of a wedding–reception. The investigation aimed to confirm the causative agent, to identify the mode of transmission and to implement appropriate preventive measures. A probable outbreak case was defined as a wedding guest with diarrhoea or vomiting with disease onset from 7 to 10 October 2012 and who consumed food at the wedding dinner prepared by a hotel in the province Salzburg on 6 October 2012. A confirmed outbreak case fulfilled the criteria of a probable outbreak case and had a laboratory-confirmed NV infection. We conducted a cohort-investigation among the wedding guests. The case definitions were fulfilled in 26 wedding guests (25 %) including 2 confirmed cases. Females were 3.2 times more likely to develop disease (95 % CI 1.4–7.2) as compared to males. A mushroom dish was found to be associated with disease risk among females (risk ratio 2.3, 95 % CI 1.2–4.3). Two of 2 tested case-patients and 6 of 14 kitchen workers tested were positive for NV GII.4 Sydney. One kitchen staff-member worked during the wedding dinner despite diarrhoea. No food safety training was documented for the employees and the kitchen staff’s restroom was lacking operational facilities for hand hygiene. We report the first investigated outbreak due to GII.4 Sydney, which was likely due to a symptomatic kitchen worker. Gender-specific eating behaviour may have posed female guests at higher risk of NV infection.     

Evaluation of amniotic fluid cell filtration: An experimental approach to early amniocentesis

Prior to a prospective application of amniotic fluid (AF) cell filtration to early amniocentesis, we tested the technique on a surplus from mid-trimester samples. By using the same sample size of 5 ml in experiments with a filter and in routine diagnostic procedures (control), we evaluated an optimal filter system. The prolonged culture time of filtered cells and the reduced number of clones are most probably due to mechanical stress (filtration pressure), whereas loss of the cells by adhesion to the filter system, and an AF-free culture medium (growth factors) are suggested to be less important. The AF cells are very sensitive to mechanical stress. Slow filtration (⩽3 ml AF/min) through filters with a high porosity and the largest possible pore size should be preferred. A mixed cellulose ester filter membrane with a pore size of 5·0 μm proved to be the most efficient, allowing harvest of the filtered cells after only a slight prolongation of the culture time (+2·4 days) compared with unfiltered aliquots. A filter set with a bypass connected by three-way taps allows cell filtration during either aspiration or reinjection of the AF. Cell filtration after amniocentesis and consecutive reverse flushing of the membrane with the appropriate amount of culture medium proved to be the best with regard to easy handling and reducing the risk of bacterial contamination.     

Waste disposal technology transfer matching requirement clusters for waste disposal facilities in China

Even though technology transfer has been part of development aid programmes for many decades, it has more often than not failed to come to fruition. One reason is the absence of simple guidelines or decision making tools that help operators or plant owners to decide on the most suitable technology to adopt. Practical suggestions for choosing the most suitable technology to combat a specific problem are hard to get and technology drawbacks are not sufficiently highlighted. Western counterparts in technology transfer or development projects often underestimate or don’t sufficiently account for the high investment costs for the imported incineration plant; the differing nature of Chinese MSW; the need for trained manpower; and the need to treat flue gas, bunker leakage water, and ash, all of which contain highly toxic elements.This article sets out requirements for municipal solid waste disposal plant owner/operators in China as well as giving an attribute assessment for the prevalent waste disposal plant types in order to assist individual decision makers in their evaluation process for what plant type might be most suitable in a given situation. There is no ‘best’ plant for all needs and purposes, and requirement constellations rely on generalisations meaning they cannot be blindly applied, but an alignment of a type of plant to a type of owner or operator can realistically be achieved. To this end, a four-step approach is suggested and a technology matrix is set out to ease the choice of technology to transfer and avoid past errors. The four steps are (1) Identification of plant owner/operator requirement clusters; (2) Determination of different municipal solid waste (MSW) treatment plant attributes; (3) Development of a matrix matching requirement clusters to plant attributes; (4) Application of Quality Function Deployment Method to aid in technology localisation.The technology transfer matrices thus derived show significant performance differences between the various technologies available. It is hoped that the resulting research can build a bridge between technology transfer research and waste disposal research in order to enhance the exchange of more sustainable solutions in future.     

Blue space: The importance of water for preference,affect, and restorativeness ratings of natural and built scenes

Although theorists have suggested that aquatic environments or “blue space” might have particular restorative potential, to date there is little systematic empirical research on this issue. Indeed the presence of water has, unintentionally, been a confounding factor in research comparing people’s reactions to built and natural environments. Whereas aquatic features (rivers, lakes, coasts) are frequently present in visual stimuli representing natural environments they are rarely incorporated in stimuli portraying built environments. As many towns are, for good reason, located near water this is a potentially significant oversight. The current research collated a set of 120 photographs of natural and built scenes, half of which contained “aquatic” elements. Proportions of “aquatic”/“green”/“built” environments in each scene (e.g. 1/3rd, 2/3rds) were also standardised. Two studies investigated preferences (attractiveness, willingness to visit and willingness to pay for a hotel room with the view), affect and perceived restorativeness ratings for these photographs. As predicted, both natural and built scenes containing water were associated with higher preferences, greater positive affect and higher perceived restorativeness than those without water. Effect sizes were consistently large. Intriguingly, images of “built” environments containing water were generally rated just as positively as natural “green” space. We propose a number of avenues for further research including exploration of the mechanisms underlying these effects.     

Reproductive functions of wild fish as bioindicators of reproductive toxicants in the aquatic environment

Background, aim, and scope  

Impacts on the reproductive health of wild fish are thought to be suitable early-warning tools indicating contamination of surface waters with endocrine-disrupting compounds. Ecotoxicological assessment of these field observations depends on the availability of reliable biomarkers to enable a discrimination of natural variations of reproductive functions from anthropogenic impacts.