Effects of two organophosphorus insecticides on some biochemical constituents in the nervous system and liver of rabbits

Male Baladi rabbits were acutely and sub-chronically intoxicated with cyanofenphos and profenophos. The levels of cholesterol, triglycerides, B-lipoproteins and total proteins were determined in the serum, brain, spinal cord and sciatic nerve of rabbits. Moreover, the activities of alkaline phosphatase, acid phosphatase and glutamic-pyruvic transaminase were determined in the liver of the animals. The whole studies revealed that the biochemical constituents were highly affected by the tested insecticides. Also, the liver function suffered from adverse effects of the tested insecticides.     

Municipal anaerobic digesters for codigestion, energy recovery, and greenhouse gas reductions.

Codigestion of five wastes and municipal wastewater sludge was evaluated using full-scale testing. Synergistic, antagonistic, and neutral outcomes were observed depending on codigestate identity and concentration, highlighting the value of careful blending. Yeast waste resulted in notable synergism, increasing biogas production by over 50%, whereas aircraft deicing waste resulted in antagonism at high loadings and neutral outcomes at lower loadings. Restaurant waste codigestion resulted in neutral outcomes. The synergisim with yeast codigestates may have resulted from trace nutrients (i.e., iron, nickel, and cobalt) in the wastes that increased microbiological activity. Antagonist outcomes with deicing waste may have been the result of organic overload or inhibitory deicer constituents. Codigestion of wastes at the feed rates investigated was estimated to produce 0.50 ML/d of methane having an energy equivalent of 17 500 MJ/d. This was estimated to reduce net carbon dioxide emissions by 560 tonnes/y.     

Lateral migration and offsite surface emission of landfill gas at City of Montreal Landfill Site

An evaluation of lateral landfill gas migration was carried out at the Saint-Michel Environmental Complex in Montreal, City of Montreal Landfill Site, Canada, between 2003 and 2005. Biogas concentration measurements and gas-pumping tests were conducted in multilevel wells installed in the backfilled overburden beside the landfill site. A migration event recorded in autumn 2004 during the maintenance shutdown of the extraction system was simulated using TOUGH-LGM software. Eleven high-density instantaneous surface monitoring (ISM) surveys of methane were conducted on the test site. Gas fluxes were calculated by geostatistical analyses of ISM data correlated to dynamic flux chamber measurements. Variograms using normal transformed data showed good structure, and kriged estimates were much better than inverse distance weighting, due to highly skewed data. Measurement-based estimates of yearly off-site surface emissions were two orders of magnitude higher than modelled advective lateral methane flux. Nucleodensimeter measurements of the porosity were abnormally high, indicating that the backfill was poorly compacted. Kriged porosity maps correlated well with emission maps and areas with vegetation damage. Pumping tests analysis revealed that vertical permeability was higher than radial permeability. All results suggest that most of the lateral migration and consequent emissions to the atmosphere were due to the existence of preferential flow paths through macropores. In December 2006, two passively vented trenches were constructed on the test site. They were successful in countering lateral migration.     

RESEARCH: Shrub Propagation Techniques for Biological Control of Invading Tree Species

Cornus stolonifera, Salix petiolaris, and Spiraea alba clones already located within the corridor of an electrical power line. To establish the efficiency of treatments, we examined the statistical differences of growth traits between species and treatments. An analysis of the effects of layering shows, after the first growth season, differences for all growth traits in only one species, Spiraea alba. After the second growth season, we observed the development of new aerial stems. Layering favors horizontal expansion of shrubs over height development. The third year after treatment, the effect of layering is reduced except for Cornus stolonifera, which continuously increases, as shown by the significant progression of the clone issued from the layer even five years after treatments. With the cutting back technique, we expected a distinct vertical growth of the shrubs at the expense of increasing the crown diameter. This technique would be best associated with the rejuvenation of clones, followed by a layering of new shoots to allow a horizontal expansion of the shrubs. Therefore, the formation of a dense shrub community by layering should be considered a valuable approach for the biological control of undesirable trees in powerline rights-of-way.     

Mikrobielle Sanierung von 2,4,6-Trinitrotoluol (TNT) kontaminierten Böden

Within the limits of a feasability study abouton-site bioremediation methods for TNT-contaminated soils, composting was chosen as a very promising and cheap method. This method was critically compared with those described in the literature and was primarily rated under ecotoxicological aspects. The investigated location is the former munition plant «Tanne» in the aerea of Clausthal-Zellerfeld in Lower Saxony, Germany. To estimate the autochtonic microflora, we assessed the number of aerobic heterotrophic bacteria and determined their respiration activity in soils. In addition, we isolated bacteria and examined their capacity to metabolize TNTin vitro. Both the amount of autochtonic microrganisms (4.7×10⁸ to 1.2×10¹⁰ colony forming units (cfu)/kg dryweight) as well as their respiration activity did not correlate with the concentrations of nitrotoluenes in the soils. With high contaminated soil (20 g TNT/kg dry weight) we carried out a small compost in the range of 10 liters. During 28 days of composting TNT-concentration decrease over 90% and only minor amounts of monoaminodinitrotoluenes were generated. However, an acidic pretreatment of the compost material at the end of the reaction showed that TNT could be partially resolved under these extreme conditions and that an ecotoxicological risk may still exist. Possible changes in the realization of the composting process in order to make sure that the contaminants are savely bound to the humin matrix are discussed.     

The influence of lindane and lindane metabolites on microsomal and mitochondrial ATPase in vitro

In vitro investigations of the influence of lindane and its metabolites were performed on microsomal and mitochondrial ATPases from liver, kidney and brain of rat and mouse. The microsomal Na+-K+-ATPases in rat liver were inhibited by the tested substances. An increase of activity was observed only with 2.5 X 10(-5) M gamma-HCH. Effects on the microsomal Na+-K+-ATPase from kidney and brain of rat were also indicated. The mitochondrial enzyme in rat liver was stimulated by all the compounds tested at concentrations of 10(-4) M - 10(-2) M. The effects on mitochondrial enzymes from kidney and brain varied in dependence on the tested substances. In the microsomes and mitochondria of mouse an influence on the Na+-K+-ATPases similar to the effects on the preparations from organs of rat was evident.