2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes migration ability of primary cultured rat astrocytes via aryl hydrocarbon receptor

Emerging evidence showed that 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) could induce expression of certain reactivation-associated genes in astrocytes, however, the consequent cellular effects and molecular mechanisms are still unclear. During the process of astrocyte reactivation, migration is a critical cellular event. In the present study, we employed wound-healing assay and Transwell® motility assay to explore the effects of TCDD on cell migration in primary cultured rat cortical astrocytes. We found that upon TCDD treatments at relative low concentrations (10^? 10 and/or 10^? 9?mol/L), the ability of primary astrocytes to migrate horizontally and vertically was promoted. In line with this cellular effect, the mRNA expression of two pro-migratory genes, including cell division cycle 42 (CDC42) and matrix metalloproteinase 2 (MMP2) was induced by TCDD treatment. Dioxin exerts its toxic effects mainly through aryl hydrocarbon receptor (AhR) pathway. So the role of AhR pathway in the pro-migratory effects of TCDD was examined using an AhR antagonist, CH223191. We found that application of CH223191 significantly reversed the pro-migratory effects of TCDD. Interestingly, the basal ability of horizontal migration as well as basal levels of CDC42 and MMP2 expression were dramatically reduced suggesting a possible physiological role of AhR in maintaining the endogenous migration ability of the primary astrocytes. These findings support the notion that dioxin promotes astrocyte reactivation at molecular and cellular levels.     

Enzyme-electrolytic degradation of dichloromethane: Efficiency, kinetics and mechanism

Enzymatic electrolysis cell(EEC) has advantages over microbial electrolysis cell(MEC) due to the needless of microbe inoculation and high-efficiency of enzymatic reaction. In this study, an EEC was first applied to achieve the effective degradation of halogenated organic pollutants and dichloromethane(CH_2Cl_2) was utilized as a model pollutant. The results indicate that the degradation efficiency of CH_2Cl_2 after 2 hr reaction in the EEC was almost100%, which was significantly higher than that with enzyme(51.1%) or current(19.0%). The current induced the continuous regeneration of reduced glutathione(GSH), thus CH_2Cl_2 was degraded under the catalysis of GSH-dependent dehalogenase through stepwise dechlorination, and successively formed monochloromethane(CH_3Cl) and methane(CH_4). The kinetic result shows that with a current of 15 mA, the maximum specific degradation rate of CH_2Cl_2(3.77 × 10~(-3) hr~(-1)) was increased by 5.7 times. The optimum condition for CH_2Cl_2 dechlorination was also obtained with pH, current and temperature of 7.0, 15 mA and 35°C,respectively. Importantly, this study helps to understand the behavior of enzymes and the fate of halogenated organic pollutants with EEC, providing a possible treatment technology for halogenated organic pollutants.     

重庆境内江河水域渔业环境特点及对船体网箱养鱼的影响

重庆境内河流属长江水系，以长江，嘉陵江，涪江等河流为骨干和主体，水域面积８．２５万ｈａ，占水域总面积的７６．４％，船体网箱养鱼是近年来在该地区兴起并迅速发展的鱼类集约化养殖方式。本文根据船体网箱养鲤试验结果和有关资料，分析了重庆境内江河水温，含氧量，流速，含沙量，浮渣，工业污染特点及对船体网箱养鱼的影响，拟合了养殖舱内，外流速关系及含沙量与透明度关系，并就船体网箱养鱼的进一步发展提出了措施。     

以可持续发展理论指导社会发展综合实验区的实践

社会发展综合实验区中中国可持续发展战略的实验基地，本文对实验区如何按可持续发展原则，结合当地的人口、资源、环境状况，制宁长远的可持续发展的生态规划做了较系统的论述。     

非枝晶半固态金属成形技术的发展概况

此文叙述了非枝晶半固态金属成形技术的发展概况。主要介绍了压铸成形、模锻成形、连续流变铸造成形、注射成形以及半固态金属锭料制造等技术的特点和应用现状。     

危险品道路运输过程风险管理体系探讨

危险品道路运输是一个复杂的系统,风险管理涉及危险品运输规划和事故应急响应。本文介绍了现有的危险品运输风险管理模式和基本管理原则,基于运输风险评估、运输路径优化、应急单位优化选址和选线、人员疏散管理以及事件决策管理等构建了危险品道路运输过程风险管理体系,阐述了系统基本要素之间的相互关系。危险品道路运输过程风险管理是一个持续改进的结构化过程风险管理体系,有助于减少危险品运输事故概率和降低运输沿线影响人员风险,为政府监管部门和危险品生产经营单位的运输安全管理、优化选线以及应急救援等工作提供技术依据,合理规划危险品运输系统。     

强化风险意识 更新管理理念 全面创建HSE管理体系

介绍了胜利采油厂在HSE管理体系建立过程中重点开展的几项工作,总结了实践中的几点体会,并对下一步体系的运行和持续改进做出了规划。     

工程系统的安全分析与风险管理

安全性是许多工程系统设计、研制和运行时要考虑的一项重要内容,其中安全分析和风险管理是两项主要的工作。在基于事故场景的安全性模型的基础上。研究了工程系统的安全分析和风险管理技术方法,提出了进行安全分析和风险管理的工作流程。     

夏季持续高温天气对北京市大气细粒子(PM2.5)的影响

在1999-06-23北京市出现长达13d的持续高温期间对细粒子(PM_2.5)质量浓度进行了观测.数据表明,持续高温期间细粒子质量浓度比非高温期间要高出2～3倍.但是通过对持续高温期间的气象数据进行分析,发现还是很利于污染物扩散.进一步分析同步监测的O₃浓度、颗粒物中SO₄^2-的粒径范围及其含量等数据,发现夏季持续高温期间活跃的光化学反应应该是北京市细粒子的主要来源.