Role of amniotic fluid interphase fluorescence in situ hybridization (FISH) analysis in patient management

We retrospectively reviewed 309 amniotic fluid interphase fluorescence in situ hybridization (FISH) analyses performed from October 1995 to June 1999 to assess the role of interphase FISH in the management of patients at increased risk for fetal aneuploidies. Gestational age and indications for amniocentesis, clinical interventions after FISH results, as well as interventions after final culture reports were analyzed. There were 244 (79%) normal, 50 (16%) abnormal and 15 (5%) inconclusive FISH results. There were no false-positive or false-negative results, but there were nine (3%) clinically significant chromosomal abnormalities not detectable by FISH. Of the 50 women with abnormal FISH results, 26 (52%) elected to terminate the pregnancy prior to the availability of the standard chromosome analysis. In two of the fetuses with trisomy 21 no abnormalities were reported by ultrasound examination. Our experience indicates that interphase FISH results played an important role in decision making, especially for pregnancies close to 24 weeks' gestation. Standard karyotype analysis is still required for detection of chromosome abnormalities not detectable by interphase FISH techniques and for clarification of unusual or inconclusive FISH results. Copyright © 2001 John Wiley & Sons, Ltd.     

Multi-scale foraging variability in Northern gannet (Morus bassanus) fuels potential foraging plasticity

The survival of marine predators depends on behavioural plasticity to cope with changes in prey distribution. Variability in behaviour might predict plasticity and is easier to assess than plasticity. Using miniaturized GPS loggers over several breeding seasons in two Norwegian Northern gannet (Morus bassanus) colonies, we investigated if and how the variability within and between individuals, but also between colonies and years, affected foraging strategies. Results revealed strong individual variability (foraging trip durations, foraging effort and different foraging areas). Individuals from both colonies showed preferred commuting routes, flight bearings and feeding hotspots. Individuals from the largest colony used larger and more foraging areas than individuals from the small colony. Feeding hotspots and foraging ranges varied amongst years in the largest colony only. Our study demonstrated that gannets show flexibility by changing prey fields that are driven by shifting oceanographic conditions.     

Biosecurity threats: the design of surveillance systems,based on power and risk

We consider the problem of designing a surveillance system to detect a broad range of invasive species across a heterogeneous sampling frame. We present a model to detect a range of invertebrate invasives whilst addressing the challenges of multiple data sources, stratifying for differential risk, managing labour costs and providing sufficient power of detection. We determine the number of detection devices required and their allocation across the landscape within limiting resource constraints. The resulting plan will lead to reduced financial and ecological costs and an optimal surveillance system.     

Public participation in NGO-oriented communities for disaster prevention and mitigation (N-CDPM) in the Longmen Shan fault area during the Wenchuan and Lushan earthquake periods

ABSTRACT

The Longmen Shan fault area in southwest China is one of the world’s most active earthquake zones. The epicenters of the two most recent earthquakes, the 2008 Wenchuan earthquake (8.0?Ms) and the 2013 Lushan earthquake (7.0?Ms), both of which caused serious losses, were only 85?km apart. Community-based disaster risk reduction is the foundation of the disaster management system pyramid and is critical to the success of ‘sustainable hazard mitigation’. Based on multiple collaborative stakeholder perspectives, this paper examines public participation in an NGO-oriented Community for Disaster Prevention and Mitigation (N-CDPM) in the period between the two earthquakes as a multi-stage problem; N-CDPM establishment, normal operations, disaster testing, and continuous improvement. Multi-stage field research was conducted in the affected areas in the Longmen Shan fault area to examine the collaboration in each stage, after which the differences were compared across the four stages based on eight key indices; scales, core stakeholders, core network stability, mean number of lines, mean collaborative level, governments, and individual and public organization participation. The government participation, individual participation, and public organization participation are then discussed. This paper provides a novel research approach to CDPM in multiple earthquake regions and gives rich insights into the collaboration between the government and the public for N-CDPM.     

The role of affective experience in work motivation: Test of a conceptual model

The purpose of this paper was to contribute to understanding of the crucial role of emotion in work motivation by testing a conceptual model developed by Seo, Barrett, and Bartunek (2004) that predicted the impacts of core affect on three behavioral outcomes of work motivation, generative-defensive orientation, effort, and persistence. We tested the model using an Internet-based investment simulation combined with an experience sampling procedure. Consistent with the predictions of the model, pleasantness was positively related to all three of the predicted indices. For the most part, these effects occurred indirectly via its relationships with expectancy, valence, and progress judgment components. Also as predicted by the model, activation was directly and positively related to effort.     

Performance comparison of structural stormwater best management practices.

This paper describes a method for comparing the pollutant removal of a number of structural stormwater treatment devices, commonly referred to as best management practices (BMPs). Historically, the pollutant removal ability of a BMP has been expressed as a percent reduction in concentration or load. Unfortunately, the calculated percent reduction in pollutant concentration is strongly affected by the influent concentration, with the calculated reduction generally being much lower when the event mean concentrations (EMCs) in the untreated runoff from the test watershed are low. The objective of the proposed methodology is to eliminate this problem by predicting BMP performance for an arbitrary influent concentration, so that BMPs evaluated in different watersheds can be compared as if the influent quality at all sites were the same. This method allows BMPs to be compared based on the quality of effluent produced and the mass reduction. The proposed method uses linear regression as the primary tool to compute the expected effluent concentration from a BMP, given a specific influent concentration of interest and was developed using data collected in the California Department of Transportation BMP Retrofit Pilot Program. This technique reveals that for media filters, the concentration of sediment and other particle-associated pollutants in treated runoff is generally unrelated to influent quality and is relatively constant. Wet basins with large permanent pool volumes also have effluent concentrations that are constant for most constituents and unrelated to influent concentrations. In these situations, the "percent reduction" in a pollutant EMC is not an inherent characteristic of the BMP, but a function of the influent EMC, because the quality of effluent produced is constant. Predicting the effluent quality of several types of conventional BMPs based on a common influent concentration allows an objective comparison of their performance and the selection of a BMP that addresses specific constituents of concern.     

Stormwater quality benefits of a porous friction course and its effect on pollutant removal by roadside shoulders.

This project has two main objectives; the first is to documents the effect of a porous friction course overlay on the quality of highway stormwater runoff, and the second is to assess pollutant removal of a vegetated area along the side of a road from both conventional and porous pavements. The quantity and quality of stormwater runoff from a four-lane divided highway in the Austin, Texas, area was monitored before and after the installation of a porous friction course (PFC). Observed concentrations of suspended solids and pollutants associated with particulate material were much lower in the runoff from the PFC compared with runoff from the conventional asphalt surface. The vegetated highway shoulder reduced the concentrations of many stormwater constituents from the conventional asphalt surface; however, the concentrations of these constituents in runoff produced by the PFC were so low that the vegetated area produced no further reduction in concentration.     

The net ecosystem carbon exchange of human-modified environments in the Australian Capital Region

As part of a larger study to quantify and map ecosystem services in southeast Australia, we estimated carbon stored in biomass and soils and the net ecosystem carbon exchange between the land surface and the atmosphere in the Australian Capital Region (ACR). Our aim was to understand and quantify how different human-modified landscapes provide an essential ecosystem service: the exchange and storage of carbon in the landscape. Using a remote sensing based modelling methodology, we obtained values of Net Primary Productivity (NPP), total carbon in soil and biomass and carbon turnover from meteorological and terrain inputs and vegetation attributes. We obtained a set of maps of NPP, total carbon (C) storage and C-turnover for the ACR. We superimposed a land use/cover map to assign the uptake, storage and release of carbon to different land use/cover types. Our results support the hypothesis that human-intensive land uses significantly affect the ability of terrestrial ecosystems to provide an important ecosystem service in the form of carbon storage.