全文获取类型
收费全文 | 1071篇 |
免费 | 96篇 |
国内免费 | 297篇 |
专业分类
安全科学 | 129篇 |
废物处理 | 19篇 |
环保管理 | 89篇 |
综合类 | 673篇 |
基础理论 | 340篇 |
环境理论 | 1篇 |
污染及防治 | 123篇 |
评价与监测 | 45篇 |
社会与环境 | 19篇 |
灾害及防治 | 26篇 |
出版年
2024年 | 1篇 |
2023年 | 16篇 |
2022年 | 23篇 |
2021年 | 39篇 |
2020年 | 18篇 |
2019年 | 17篇 |
2018年 | 30篇 |
2017年 | 36篇 |
2016年 | 42篇 |
2015年 | 44篇 |
2014年 | 34篇 |
2013年 | 104篇 |
2012年 | 85篇 |
2011年 | 90篇 |
2010年 | 68篇 |
2009年 | 66篇 |
2008年 | 78篇 |
2007年 | 91篇 |
2006年 | 78篇 |
2005年 | 65篇 |
2004年 | 55篇 |
2003年 | 58篇 |
2002年 | 46篇 |
2001年 | 40篇 |
2000年 | 35篇 |
1999年 | 28篇 |
1998年 | 27篇 |
1997年 | 22篇 |
1996年 | 25篇 |
1995年 | 22篇 |
1994年 | 22篇 |
1993年 | 13篇 |
1992年 | 9篇 |
1991年 | 8篇 |
1990年 | 4篇 |
1989年 | 6篇 |
1988年 | 9篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1978年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有1464条查询结果,搜索用时 46 毫秒
31.
Thirty-six pregnancies in 25 families at risk of β-thalassaemia major received prenatal diagnosis. Chorionic villus sampling or amniocentesis was done in 35 pregnancies to obtain fetal cells for DNA linkage study, for which Southern blotting and DNA hybridization were used to detect seven restriction fragment length polymorphisms (RFLPs) within the β-globin gene cluster: ϵ-HincII, Gγ-HindIII, Aγ-HindIII, Φβ-HincII, 3′Φβ-HincII, β-AvaII, and 3′β BarnHI. β-Thalassaemia major was diagnosed in seven and excluded in 22 pregnancies. In the remaining six cases, β-thalassaemia major could not be excluded. In these six pregnancies and another one with late booking, ultrasound-guided cordocentesis was performed at the 22nd to 27th week of gestation. Globin chain composition was determined with urea-acetic acid-Triton X-100-12 per cent polyacrylamide gel electrophoresis. β-Thalassaemia major was diagnosed in two fetuses and excluded in the other five. Eleven fetuses (in which β-thalassaemia major was excluded) have been delivered and are healthy at more than 5 months old DNA linkage analysis coupled with globin chain electrophoresis provides an effective way for prenatal diagnosis of β-thalassaemia major, although these methods are being replaced by more direct detection techniques using oligonucleotide probes. 相似文献
32.
A syncytiotrophoblast-associated antigen identified by the monoclonal antibody (McAb) H315 is detectable on the surface of a low proportion of peripheral blood cells in pregnant women, raising the possibility of a new approach to prenatal diagnosis of genetic disorders. We aimed at verifying the trophoblastic origin of H315+ cells and their use for prenatal diagnosis of β-thalassaemia. H315 + cells were separated from the peripheral blood of pregnant women: the DNA obtained from these cells in two selected cases was shown to have genetic markers indistinguishable from those of the mother and definitely different from the fetus. Our results suggest that H315 antigen is expressed by maternal cells and that prenatal diagnosis on peripheral blood of the mother using H315 McAb is not feasible. 相似文献
33.
Wang Liming Zhang Junwu Wu Guanyun Wang Shenwu Fan Yu Huang Youwen Zhao Letian Wang Rongxin Su Jidong Zhang Nijia Long Guifang Li Qi Zou Peng Liang Rong Liang Xu 《黑龙江环境通报》1986,6(2):89-95
By means of chorion biopsy together with restriction endonuclease analysis of fetal DNA, first trimester diagnoses were successfully made in 33 fetuses at risk for Bart's hydrops fetalis. Seven pregnancies with Hb H or hydrops fetalis were therapeutically terminated before 4 months of gestation. Of the 26 pregnancies intended to continue, 18 have come to term with normal deliveries; one with threatened abortion was terminated at the end of the first trimester and, seven are progresssing normally. 相似文献
34.
应用液相色谱结合电化学检测技术,研究了标准石英粉尘和青石棉体外诱导DNA分子氧化损伤产生加合物8-羟基脱氧鸟苷的形成与机制。结果表明,石英粉尘和青石棉均可致DNA分子氧化损伤并形成8-羧基脱氧鸟苷,但青石棉作用强于石英粉尘;石英粉尘的青石棉对H2O2氧化DNA的催化能力有较大差异。标准石英粉尘和青石棉,具有直接的遗传毒性;两者诱导DNA分子形成强致突变的8-羧基鸟苷可能是其潜在致癌机制之一。 相似文献
35.
重大环境污染事故的防范及紧急处置 总被引:2,自引:0,他引:2
重大环境污染事故危害重大,为及时防范和处置,应对其隐患者监护和事故性风险评估,建立紧急救助的快速反应系统和紧急处置询系统,开发专家系统,强化专业人员培训并建立国家的安全信息数据库。 相似文献
36.
37.
E. Keller Dipl.-Biol. A. Andreas S. Scholz H. C. Dörr D. Knorr E. D. Albert 《黑龙江环境通报》1991,11(11):827-840
In 19 pregnancies at risk for 21-hydroxylase deficiency (21OHD) in 18 families with at lea one affected child, prenatal diagnosis was performed by RFLP analysis using the enzymi Taq I and EcoRI and the DNA probes specific for the 21 OH genes, the closely linke complement C4 genes and the highly polymorphic HLA class II genes DRB, DQB, and DPI For fetal DNA analysis either chorionic villi or cultivated amniotic cells were used. In all 1 cases, a clear prenatal diagnosis was possible either with the 21OH probe alone or in mo cases, by combining the results of the different closely linked loci. 相似文献
38.
39.
天然水体中有机物检测方法的选择与优化 总被引:2,自引:0,他引:2
本文选取并优化了一种准确、易行、适合于天然水样中微量有机物的分析技术.天然水样品中有机物通过混合大孔径吸附树脂XAD-2与XAD-7(4:1,V/V)富集并用二氯甲烷洗脱.所有富集的有机物样品中均加入衍生化试剂N,O-双(三甲基硅基)三氟乙酰胺(缩写为BSTFA)硅烷化,最后采用气相色谱-质谱联用仪分析.为了实验的简便,我们的样品为模拟水样(4L添加定量5β-胆烷(挪威Chiron公司)的去离子水). 相似文献
40.
M. Schwartz M. Super J. Schmidtke C. Buys M. Farrall D. Halley M. Krawczak J. E. Poncin D. Loukopoulos M. Devoto 《黑龙江环境通报》1988,8(8):619-624
This paper presents data collected in Europe on 107 prenatal diagnoses of cystic fibrosis (CF) using linked DNA markers. To date, 38 children have been born without CF, as predicted, demonstrating the present rapid move from research to clinical genetic service. 相似文献