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101.
E. Bitoun C. Bodemer J. Amiel Y. de Prost C. Stoll P. Calvas A. Hovnanian 《黑龙江环境通报》2002,22(2):121-126
Netherton syndrome (NS) is a severe autosomal recessive ichthyosis with no specific treatment or prenatal diagnosis available at present. The recent identification of SPINK5, which encodes a serine protease inhibitor, as the defective gene enables DNA-based prenatal diagnosis to be carried out. Here we report the first direct molecular prenatal diagnosis of a lethal form due to a recurrent SPINK5 mutation in three consanguineous Turkish families. XmnI restriction enzyme digestion and DNA sequencing demonstrated that each deceased affected child was homozygous for mutation 153delT inherited from each parent. Analysis of fetal DNA from amniotic fluid cells in Family 1 and from a chorionic villus sampling in Family 3 showed that the fetus was heterozygous for 153delT in both cases. The pregnancies were carried to term and the newborns were unaffected. In Family 2, fetal DNA analysis from chorionic villus biopsy showed in a first pregnancy that the fetus was homozygous for 153delT. The pregnancy was terminated at 13 weeks and DNA analysis of fetal keratinocytes confirmed the prenatal prediction. In a second pregnancy in Family 2, fetal DNA analysis showed heterozygosity for 153delT, and the pregnancy was continued. Direct SPINK5 mutation analysis in families at risk for NS represents the first early, rapid and reliable method for prenatal diagnosis of this life-threatening form of ichthyosis. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
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大豆皂甙复合物抑制猴免疫缺陷病毒活性的观察 总被引:9,自引:0,他引:9
以猴免疫缺陷病毒(SIV)为靶病毒和相应的CEMx174细胞系统为模型,以AZT为阳性对照药物,观察了从大豆提取的皂甙复合物及大豆胰蛋白酶抑制剂对细胞病变的影响.结果表明,大豆皂甙复合物具有明显的抗SIV作用,而大豆胰蛋白酶抑制剂则无抗SIV作用. 相似文献
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有色冶炼重金属废水回用研究 总被引:6,自引:0,他引:6
通过对株洲冶炼厂重金属废水回用结垢问题的研究,针对其垢样主要属碳酸钙垢型的特点,提出以投加高效低剂量的阻垢剂作为防止结垢的主要方法,运行证明,该防止结垢方法具有明显的经济效益和环境效益,应用前景广阔。 相似文献
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将磷霉素生产盐渣经水解得到右旋磷霉素二钠,再通过亲核取代反应得到可用作循环冷却水阻垢剂的氨基二乙基(1-甲基-2-羟基)膦酸四钠(ADMHP·Na4),并用FTIR表征了产物结构.实验结果表明:在取代反应温度为25℃、取代反应时间为2h、n(氨水)∶n(右旋磷霉素二钠)=8的条件下,由右旋磷霉素二钠制备ADMHP·Na4,收率可达83.87%.处理1t盐渣可创造利润3 080元.当ADMHP·Na4质量浓度为30 mg/L,水样温度为60℃时,ADMHP·Na4对CaCO3的阻垢效果较好,阻垢率可达91.47%. 相似文献
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Zhao-xia Song Hong-wu He Yao-ting Fan Hong-wei Hou 《International Journal of Green Energy》2017,14(3):330-335
The redox state of carbon sources directly affected the ratio of NADH/NAD+ which was coupled to the hydrogen production by Bacillus sp. FS2011. The addition of the inhibitor of pyruvate dehydrogenase multi-enzyme complex (PDHc)E1 could regulate hydrogen production by FS2011 or pretreated compost in batch cultivation. With the addition of appropriate amount of inhibitor, hydrogen production via the NADH pathway was increased, leading to the higher overall hydrogen production. The maximum hydrogen yields of 307.6 ± 13.21 mL/g by FS2011 with the inhibitor of 80 ppm and 362.1 ± 10.1 mL/g by pretreated compost with the inhibitor of 60 ppm were observed, which were increased by 8.7% and 17.8% compared with the controls, respectively. Meanwhile the production of soluble metabolic byproducts such as butyrate, acetate and so on were decreased, resulting in reducing the difficulty of wastewater treatment. 相似文献
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