基于浮游生物群落变化的生态学基准值计算方法初探

基于浮游生物群落对环境压力变化的响应,参照美国国家环境保护局(US EPA)颁布的生物学基准计算方法,结合理化因子,采用综合指数法计算了我国太湖流域和辽河流域的生态学基准值.计算框架为:①确定流域水环境生态学基准参照点和生态学基准指标;②作出参照点各基准指标box分布图并进行评分;③将各生态学基准指标得分等权重相加,并把90分位数值作为该流域的基准值.结果表明:我国太湖流域夏、冬季的生态学基准值分别为94.7和86.7,辽河流域夏、冬季的生态学基准值分别为100.0和96.4.通过该基准值可较好地区分2009年辽河流域和太湖流域夏、冬季采样点位的生境优劣,这说明基于浮游生物群落变化来计算生态学基准值的方法是可行的.      

气井完整性概念初探及评价指标研究

针对目前国内外尚无明确的气井完整性概念和生产气井完整性评价指标体系的现状,分析了现有的关于气井完整性解释的不足,初步提出了气井完整性的概念,强调了气井完整性是气井处于地层流体发生无控制流动的风险在可接受范围之内的状态。并根据此概念,从完整性管理、屏障设备、监测状态三个方面,建立了气井完整性评价指标体系,分析了各个评价指标与气井完整性的关系。屏障设备完整性是气井完整性的核心,完整性管理是提高气井完整性的关键,监测状态是气井完整性最直接的判断依据。     

Population structure and community characteristics of Acer catalpifolium Rehd北大核心CSCD

Acer catalpifolium Rehd., a critically endangered tree species with an extremely limited range of distribution, is one of the 120 plant species with extremely small populations, as approved by the state forestry administration of the People's Republic of China and requires urgent rescue action. In order to comprehensively understand the population status and the future developmental trend of A. catalpifolium, the plant communities were investigated from 5 sites, including Caishenmiao (CSM), Banruosi (BRS), Zhangshancun (ZSC), Fuhusi (FHS), and Baoguosi (BGS). The population structure of A. catalpifolium as well as the species composition and community characteristics of its habitat were investigated. The results showed that A. catalpifolium is mainly distributed in the evergreen broad-leaved and deciduous broad-leaved mixed forests, in different community layers, namely, the tree layer, shrub layer, and herb layer, and is accompanied by 52, 74, and 52 plant species, respectively. Analyses of the distribution of population abundance revealed that BRS had the largest distribution of A. catalpifolium, accounting for 26.04% of the total population, followed by FHS, ZSC, BGS, and CSM, in that order. Analyses of the community characteristics revealed that the species diversity indices in FHS, BRS, BGS, and CSM were greater than that in ZSC. Analyses of the population age structure of A. catalpifolium revealed the gap in the distribution of the levels of seedlings and young trees. There were serious obstacles to the regeneration of the natural population. We concluded that the obstacle to the regeneration of the population of A. catalpifolium might be caused by the high competitive pressure from the dominant species and the micro-environment in the forest. Understanding the community characteristics and the population structure of A. catalpifolium could provide a theoretical foundation for its reintroduction and recovery. © 2018 Science Press. All rights reserved.     

Effects of water velocity on bulk water quality and biofilm population structure in drinking water distribution systems北大核心CSCD

To study the effect of flow velocity on drinking water distribution systems, bulk water quality was monitored over 28 days, biomass was measured, and 16S rDNA was sequenced on the 28th day using a water distribution simulation system. The relationship between bulk water quality and biofilm was statistically analyzed. Flow velocity of 0.5 m/s yielded the most total organic carbon (TOC) (5.26 ± 0.17 mg/L) in the bulk water, the most bulk water bacteria (lg (n+1/mL-1) = 4.79 ± 0.02), the worst bulk water quality, and the most biofilm bacteria (lg (n+1/cm-2) = 5.48 ± 0.06). A Pearson correlation analysis showed the total number of biofilm bacteria was positively correlated with conductivity (R = 0.73, P < 0.01), turbidity (R = 0.87, P < 0.001), TOC (R = 0.94, P < 0.001), and total bacteria (R = 0.92, P < 0.001), and was negatively correlated with residual chlorine (R = -0.68, P < 0.05). Biofilm diversity was high under the low (0.1 m/s) and high (2.5 m/s) flow rates, but the bacterial diversity of biofilm was the lowest at the 0.5 m/s flow rate, in which Proteobacteria dominated the biofilm community structure. These results suggest that flow velocity affects bulk water quality and biofilm population structure, and water quality and biofilm population structure are interrelated, which provides the theoretical basis for research on biofilms in drinking water distribution systems. © 2018 Science Press. All rights reserved.     

Community structure and biotic integrity of aquatic plants along the North Canal in Beijing北大核心CSCD

Aquatic plants along the North Canal in Beijing were studied to identify the community structure of aquatic plants and vegetation index of biotic integrity (VIBI), and to provide scientific basis for the management and protection of urban rivers. Aquatic plants from 49 sampling sites along the North Canal were investigated during June 2015. Based on the field data, distributing range analysis, discriminatory power analysis, and correlation analysis were used stepwise to select core metrics from candidate metrics to establish the VIBI assessment system. The VIBI value of each sampling site was calculated as the average of the scaled values of all core metrics. Thirty-six aquatic plant species, including 14 hygrophytes, 13 emergent species, 6 submergent species, 2 floating-leaved species, and 1 floating species were collected. Species diversity was low in the North Canal, and no aquatic plants were recorded in 28 sampling sites, of which 9 sampling sites were dried up. Five sites were in excellent condition (VIBI > 0.60), 5 were good (0.60 > VIBI > 0.38), 7 were fair (0.38 > VIBI > 0.23), and 4 were poor (VIBI < 0.23). Based on the distribution of VIBI, Shahe River and Wenyu River upstream, and Fenghe River located in suburbs had a higher VIBI. Downstream tributaries, such as Qinghe River, Bahe River, and Liangshui River, had a lower VIBI. Correlation analysis showed that habitat quality, habitat complexity, and vegetation diversity along riparian zones were the important factors affecting VIBI along the North Canal, Beijing. Aquatic plants along the North Canal showed low species diversity owing to human disturbance. VIBI along tributaries with limited disturbance from human activities was higher; however, VIBI along tributaries disturbed by frequent human activities was lower. © 2018 Science Press. All rights reserved.     

Mitigating the Davos dilemma: towards a global self-sustainability index

The ‘Davos dilemma’ posits a sustainability crisis, provoked by rising human population and intense competitive behaviours, in terms of control and access to depleting natural resources. More broadly understood as an ecological problem, rather than just socio-economic behavioural deficiencies, the call is for better integrated social, natural and business-indexed reporting within planetary boundaries. This poses challenges for nationally governed societies to equitably account for self-sustainability performance, in enabling their successive government agendas to re-orientate policies and industry investments as innovation towards achieving this in the longer term. We propose and test a global self-sustainability index for countries across four metrics: economic, environmental, social and innovation. Our tentative findings from a cross-country analysis of 27 countries during 2007–2010 illustrate the approach for wider systematic analysis and as a basis for future large-scale assessments on self-sustainability within and between countries.     

基于着生硅藻指数的梧桐河水生态健康评价

在黑龙江省梧桐河,调查了13个点位的着生硅藻群落结构和水环境特征,应用8种硅藻指数对该河流水生生态系统进行健康评价,并在此基础上应用主成分分析、相关分析、冗余分析、箱型图分析等统计分析方法研究了影响梧桐河着生藻类群落的主要环境因子和8种硅藻指数在梧桐河的适用情况。结果显示:梧桐河存在一定程度的环境污染,优势种以小型异极藻(Gomphonema parvulum)等耐污种为主。8个硅藻指数中,硅藻属指数(Generic Index of Diatom,GI)、澳大利亚河流硅藻生物评价指数(Diatom Index for Australian Rivers,DIAR;Diatom Species Index for Bioassessment of Australian Rivers,DSIAR)评价结果偏差较大,在梧桐河不适用;相似度指数(Jaccard Index,JI)与富营养化硅藻指数(Trophic Diatom Index,TDI)评价结果较为严格,评分明显偏低;硅藻污染耐受指数(Pollution Tolerance Index,PTI)、硅藻生物评价指数(Diatom Bioassessment Index,DBI)和生物硅藻指数(Biological Diatom Index,BDI)在梧桐河流域适用性最好。根据冗余分析(RDA),将梧桐河流域点位分为3组,第一组点位为中-重度污染,主要影响因子为流速和总磷;第二组点位为轻-中度污染,主要影响因子为磷酸盐、氨氮和溶解氧;第三组点位为轻污染-清洁,主要影响因子为p H和电导率。     

应用鱼类生物完整性指数评价荔浦河河流健康

生物完整性指数是河流生态系统健康评价主要方法之一。为评价荔浦河河流健康状况,选择鱼类作为指示生物,构建了基于鱼类生物完整性指标体系。2017年1月、4月、7月和10月对荔浦河4次采样共采集到鱼类21 192尾,经鉴定共计94种,隶属于6目17科62属。以S1(修仁镇)、S10(马岭镇)、S11(双江镇)作为参考点,经过分布范围分析、箱体图判别能力分析及相关性分析等指标筛选过程从25个候选指标筛选出5个指标,即鱼类总物种数、Shannon-Wiener多样性指数、肉食性鱼类数量百分比、敏感性鱼类数量百分比、产漂浮型卵鱼类数量百分比。将荔浦河河流健康等级分为"健康"、"一般"、"较差"、"极差"和"无鱼"5个等级。结果表明,荔浦河青山镇、荔浦县及蒲芦乡河段健康状态为"一般"水平,东昌镇、龙怀乡、杜莫镇及新坪镇河段健康状态为"较差"水平,茶城乡河段健康状态为"极差"水平。筑坝工程、架桥工程和修路工程等人类活动导致荔浦河支流上的健康状况比干流更差。上述研究结果可为荔浦河的河流管理和保护提供理论依据和技术支持。     

环境DNA(eDNA)宏条形码技术对枝角类浮游动物物种鉴定及其生物量监测研究

环境DNA(eDNA)宏条形码(Metabarcoding)技术越来越多地被应用于环境中物种定性识别,但如何定量监测物种在环境中的丰度尚未得到解决。本研究以太湖流域常见的5种浮游动物拟同形溞、大型溞、蚤状溞、多刺裸腹溞、老年低额溞为研究对象,建立了一种基于eDNA宏条形码技术的物种定量方法,并与实时荧光定量PCR(qPCR)相比较,研究了eDNA宏条形码技术多物种定量的准确性。结果表明,PCR引物对eDNA宏条形码的物种检测和定量影响显著。313 bp COI313引物对浮游动物物种覆盖度高,但是物种间DNA扩增的偏好性大,不适用于eDNA宏条形码定量检测。基于COI序列重新设计的短COI116引物能够同时检测出所有5个物种。荧光定量PCR(qPCR)物种拷贝数与物种相对占比呈正相关。eDNA宏条形码所检出每个物种的序列数与qPCR定量拷贝数高度一致。综上,eDNA宏条形码技术可实现对浮游动物物种的半定量检测,在生物多样性监测和生物完整性评价有显著的应用价值。     

氟离子慢性暴露对中国林蛙蝌蚪生长发育的毒性效应研究

氟元素以不同的结合形式广泛存在于自然界中,适量氟的摄取有益于龋齿预防和骨骼发育,然而过量氟的摄取会对动物及人体健康造成危害。近年来,大量的人类活动导致水环境中氟含量持续升高。为探究水域氟污染对中国林蛙的毒性影响,本研究以中国林蛙(Rana chensinensis)胚胎为试验材料,对卵黄栓期(G12期)胚胎进行了0、0.7、4.2、19.4、42.8 mg·L~(-1)F-慢性水体暴露直至胚胎发育到变态高峰期(G42期)的研究。分别于暴露25 d和40 d后取样测定了蝌蚪全长、体长、体重和发育分期;此外,分析了F-慢性暴露对变态率、G42期蝌蚪的全长、体长、体重和后肢长以及G42期蝌蚪骨骼发育的影响。结果表明:暴露25 d时,4.2 mg·L~(-1)F-处理组促进了林蛙蝌蚪的生长发育,而42.8 mg·L~(-1)F-处理组显著抑制了蝌蚪的生长发育;暴露40 d时,19.4 mg·L~(-1)F-和42.8 mg·L~(-1)F-处理组蝌蚪的生长发育均受到显著抑制。持续进行慢性暴露78 d后,4.2 mg·L~(-1)F-处理组蝌蚪的变态率显著升高,而42.8 mg·L~(-1)F-处理组蝌蚪的变态率受到了显著抑制。此外,42.8 mg·L~(-1)F-处理组G42期蝌蚪形态指标(全长、体长和后肢长)以及骨骼发育均受到抑制。依据G42期中国林蛙蝌蚪的生长发育指标和变态率为观察指标,氟离子慢性暴露对中国林蛙蝌蚪的最低可观察效应浓度(LOEC)为0.7 mg·L~(-1)。研究表明,水环境中高浓度的氟污染会对中国林蛙蝌蚪的生长发育、变态和骨骼发育等造成潜在的不利影响,水体氟污染的生态毒性效应理应引起高度重视。