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141.
A. Minelli M. Piantanida G. Simoni F. Rossella L. Romitti B. Brambati C. Danesino 《黑龙江环境通报》1992,12(11):959-963
Nine pregnancies at risk for various metabolic disorders were monitored by prenatal diagnosis on chorionic villi obtained between the sixth and ninth weeks of pregnancy. A diagnosis of an affected fetus was made in five cases (Sandhoff, Tay—Sachs (2), Pompe's, GMI), while metachromatic leukodystrophy, GM1 (2), and Pompe's were excluded in four cases. It is concluded that chorionic villi are a reliable tissue for prenatal diagnosis of metabolic disorders also when obtained before the ninth week. 相似文献
142.
Livia Poenaru Christine Mezard Said Akli Jean-François Oury Yves Dumez Joëlle Boue 《黑龙江环境通报》1990,10(4):231-235
We investigated the possibility of prenatal diagnosis of mucolipidosis type II (ML II) by lysosomal enzyme determination on amniotic fluid obtained at 11 weeks of gestation in three pregnancies at risk. Diagnosis of ML II was made in one case on the basis of increased levels of five lysosomal enzymes tested. The diagnosis was confirmed on cultured chorionic cells, their cultured medium, 17–week amniotic fluid, and fetal plasma obtained for confirmation prior to the termination of pregnancy. 相似文献
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144.
在实验生态条件下,研究浓度范围从0.1-50μg/L的苯并(a)芘和芘的等浓度混合物暴露对梭鱼(Mugil so-iuy)肝脏抗氧化酶活性的影响。结果显示,对肝脏谷胱甘肽过氧化物酶(GPx)和超氧化物歧化酶(SOD)活性的影响主要表现出抑制效应。在暴露过程中,抗氧化酶活性也有出现短暂的诱导,高浓度组出现诱导的时间比低浓度组早。SOD和GPx活性的变化有一定的同步性。CAT活性在暴露早期出现诱导。抗氧化酶活性的变化间接反映了环境中氧化胁迫的存在。 相似文献
145.
Low levels (ng/g) of musk ketone (MK),used as a fragrance additive in the formulation of personal care products,are frequently detected in the water and other environment.Thus,aquatic organisms can be continuously exposed to MK.In this study,kinetics and dose-response assessments of 2-amino-MK (AMK) metabolite,bound to cysteine-hemoglobin (Hb) in rainbow trout,formed by enzymatic nitro-reduction of MK have been demonstrated.Trout were exposed to a single exposure of 0.010,0.030,0.10,and 0.30 mg MK/g fish... 相似文献
146.
在纯培养条件下,研究了外生菌根真菌红绒盖牛肝菌(Xerocomus chrysenteron)对不同浓度DDT的生长效应、耐受性和氧化酶活性,测定了在DDT浓度为80.0 mg·L-1液体培养条件下菌种生物量积累和漆酶活性随培养时间的变化. 结果表明,不同浓度DDT处理并不会改变被试菌种的生长模式,所有处理组均为典型的Logistic增长;Xerocomus chrysenteron对DDT胁迫有很好的耐受性,其半抑制浓度可达139.75 mg·L-1;在80.0 mg·L-1液体培养条件下,Xerocomus chrysenteron生长正常,且36 d后培养液中DDT残留率仅为初始添加量的3.5%;在高浓度DDT胁迫下,被试菌种的漆酶和过氧化物酶活性显著增强,但液体培养条件下漆酶从第16 d开始出现,36 d后培养液中漆酶活性和比活力分别达到107.24 U·L-1和61.77 U·g-1.外生菌根真菌Xerocomus chrysenteron通过不同方式来响应DDT胁迫,显示出生物降解甚至矿化DDT的巨大潜力. 相似文献
147.
Mi Ni Qiong Wu Junli Wang Jian H. Ren Dian P. Zhang 《Journal of environmental science and health. Part. B》2013,48(12):777-785
AbstractBacillus spp. have long been used as biocontrol agents because of their efficient broad-spectrum antimicrobial activity. We identified a novel strain of Bacillus atrophaeus, named JZB120050, from soil. B. atrophaeus JZB120050 had a strong inhibitory effect against Botrytis cinerea and many other phytopathogens. Gas chromatography-mass spectrometry showed that B. atrophaeus JZB120050 produced many secondary metabolites, such as alkanes, alkenes and acids; some of which were related to pathogen inhibition. Enzyme activity analysis showed that B. atrophaeus JZB120050 secreted cell-wall-degrading enzymes, including chitinase, glucanase and protease, which degraded fungal cell walls. Both the novel glucanase gene bglu and chitinase gene chit1 were cloned and heterologously expressed in Escherichia coli and the products showed strong enzyme activity. In addition, B. atrophaeus JZB120050 secreted siderophores and formed a significant biofilm. Future studies should focus on these antimicrobial factors to facilitate widespread application in the field of agricultural biocontrol. 相似文献
148.
149.
镉敏感水稻突变体在镉胁迫下活性氧代谢的变化 总被引:11,自引:2,他引:9
从粳稻中花11为受体构建的经农杆菌介导的T-DNA插入水稻突变体库中获得了1个对镉敏感的突变体,野生型和突变体经Cd2+胁迫表型上有显著差异.结果表明:镉敏感水稻突变体地上部对Cd2+吸收与转运速率要快于野生型,膜脂过氧化程度更高;随Cd2+胁迫浓度的增加,O2·-和H2O2在叶片中的积累增加,诱导抗氧化代谢;敏感突变体叶片SOD的抗氧化胁迫能力是有限的,与野生型相比突变体叶片CAT受到更显著的抑制,可能是其对Cd2+胁迫更加敏感的生理原因.而叶片G-POD活性整体差异不显著. 相似文献
150.