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941.
Azotobacter vinelandii UWD, ATCC 53799, an engineered strain derived from Azotobacter vinelandii UW was used in the poly(ethylene glycol) (PEG)-modulated synthesis of poly(-hydroxybutyrate) (PHB). To the best of our knowledge, this is the first report on modulating the production of PHB by amending the fermentation broth with PEG using A. vinelandii UWD. It was determined that A. vinelandii UWD is prone to back-mutation to the parent strain; hence fermentation experiments require the use of the antibiotic rifampicin. Diethylene glycol (DEG) and PEGs with molecular weights of 400, 2000, and 3400 Da and pentaerythritol ethoxylate (PEE) were used in the modulated fermentation experiments in a concentration of 2% (w/v). The molecular weight of the resulting polymers was reduced by up to 78%. No impact on the productivity of the strain was observed. Spectroscopic evidence showed that PEG-modulated synthesis resulted in the covalent attachment of the ethylene glycol moiety only when a small molecule, DEG, was used. PEGs had the same effects on the polymer formation in terms of molecular weight reduction as DEG, but no spectroscopic evidence was found for the formation of a covalent linkage between PHB and higher molecular weight PEGs. 相似文献
942.
Caroline Michel Marie-Thérèse Giudici-Orticoni Frauke Baymann Mireille Bruschi 《Water, Air, & Soil Pollution: Focus》2003,3(3):161-169
The treatment of soils and ground waters polluted by heavy metals is of economical and environmental interest. Reduction of Cr(VI) to the less toxic Cr(III) associated to its precipitation is a potentially useful process for bioremediation. In order to develop ecological processes using micro-organisms, we have compared various sulfate-reducing bacteria for enzymatic reduction of chromate. The best Cr(VI) reductase activity was obtained with Desulfomicrobium norvegicum. Despite morphological changes induced by the presence of chromate, this strain can grow in the presence of up to 500 M Cr(VI) and can decontaminate waters polluted by Cr(VI) when seeded in bioreactors. We have demonstrated the ability of several metalloenzymes (cytochromes c
3 and hydrogenases) to reduce chromate. Biophysical investigations of the chromate/protein interaction in order to get further informations on the mechanism of metal reduction by cytochromes c
3 are under the way. 相似文献
943.
Emergency seed aid in Kenya: some case study insights on lessons learned during the 1990s 总被引:1,自引:0,他引:1
Sperling L 《Disasters》2002,26(4):329-342
This article reviews the effectiveness of seed-aid distributions in Kenya during the 1990s. It analyses the internal process and effects, i.e. the performance of the aid itself as well as the external process and effects, i.e. how seed-aid intervention affected farmers' broader agricultural management strategies. During the drought emergency of 1997, Kenyan farmers favourably judged many of the immediate seed-aid features such as crop and variety appropriateness and seed quality--even through the overarching goals of the seed assistance were muddled, ranging from assistance to the poor, to generalised gift-giving to stimulating progressive farming practice. However, the longer term analyses, drawn from recollections of a decade of relief activity, showed no concrete evidence that seed aid, per se, had strengthened their farming systems, nor that those who have received it once were less likely to receive it again. Thus, while seed aid has been promoted to lessen the effects of an 'acute' stress, drought, Kenyan farmers, in practice, have been experiencing much wider, 'chronic' seed system problems. This article ends by exploring this distinction between acute and chronic seed system stress and suggests a range of interventions appropriate to each. 相似文献
944.
Anderson AB 《Environmental management》2002,29(3):428-436
The Land Condition Trend Analysis (LCTA) program is the US Army's standard for land inventory and monitoring, employing standardized
methods of natural resources data collection, analyses, and reporting designed to meet multiple goals and objectives. Critical
to using LCTA data in natural resources management decisions is the ability of the LCTA protocols to detect changes in natural
resources. To quantify the ability of LCTA protocols to detect resource changes, power analysis techniques were used to estimate
minimum detectable effect sizes (MDES) for selected primary and secondary management variables for three Army installations.
MDES for a subset of primary variables were estimated using data from 27 installation LCTA programs. MDES for primary and
secondary variables varied widely. However, LCTA programs implemented at larger installations with lower sampling intensities
detected changes in installation resources as well as programs implemented at smaller more intensively sampled installations.
As a national monitoring program that is implemented at individual installations, LCTA protocols provide relatively consistent
monitoring data to detect changes in resources despite diverse resource characteristics and implementation constraints. 相似文献
945.
Methods for life cycle assessment of products (LCA) are most often based on the general prevention principle, as opposed to the risk minimization principle. Here, the desirability and feasibility of a combined approach are discussed, along with the conditions for elaboration in the framework of LCA methodology, and the consequences for LCA practice. A combined approach provides a separate assessment of above and below threshold pollution, offering the possibility to combat above threshold impacts with priority. Spatial differentiation in fate, exposure, and effect modelling is identified to play a central role in the implementation. The collection of region-specific data turns out to be the most elaborate requirement for the implementation in both methodology and practice. A methodological framework for the construction of characterization factors is provided. Along with spatial differentiation of existing parameters, two newly introduced spatial parameters play a key role: the sensitivity factor and the threshold factor. The practicability of the proposed procedure is illustrated by an example of its application. Providing a reasonable data availability, the development of separate LCA characterization factors for the respective assessment of pollution levels above and below environmental threshold values seems to be a feasible task that may add to LCA credibility. 相似文献
946.
应用核磁共振技术找水是核磁共振技术应用的新领域,核磁共振找水是一种直接探测地下水的地球物理方法。本文介绍了在贫水地区一个指定范围内探测地下水例子。比较所做的三个僚共振测点的工作结果,确定出含中水情况较好部位,配合做了率对四极测探,其工作结果指示出一低阻区域,是地下水富集带。在设计的钻井位置钻探探打出了优质 下水,验证了核磁共振找水方法的推断结果。在对该磁共振找水方法研究和实践的基础上,指出:核磁共 相似文献
947.
超临界水氧化中设备腐蚀的研究现状 总被引:2,自引:0,他引:2
超临界水氧化(SCWO)技术是近年发展起来的处理有机难溶废物的安全高效的新技术。在阐述超临界水的物理、化学性质的基础上,介绍了SCWO反应的工作原理和流程,分析总结了世界范围内开展的SCWO环境中设备腐蚀及监测技术等方面的研究动态,提出了我国SCWO技术研究的未来发展方向。 相似文献
948.
949.
950.
Status of recycling end-of-life vehicles and efforts to reduce automobile shredder residues in Korea
Hyun-Tae Joung Sung-Jin Cho Yong-Chil Seo Woo-Hyun Kim 《Journal of Material Cycles and Waste Management》2007,9(2):159-166
The present end-of-life vehicle (ELV) recycle rate and management status during the dismantling stage were investigated to
aid the establishment of policies for the management of ELVs by surveying information and using the results gained from questionnaires
given to dismantlers. The average recycle rate at the dismantling stage was 44% of the mass of a new vehicle and the rest
of the ELV was then compressed and transported to shredding companies to recover mainly the iron content, which averaged 38.7%
of the mass of a new vehicle. Nonferrous metals such as copper, antimony, zinc, and aluminum accounted for only 1.5%. The
automobile shredder residues (ASRs) were composed of light and heavy fluffs and soil/dust and amounted to 15.8% based on the
mass of a new vehicle. The dumping of fluff and inorganic residues in landfill sites, however, will be restricted when new
regulations are implemented to reduce the disposal amount to less than 5% of a new car, as has been done in European countries
and in Japan. The detailed characteristics of ASR were investigated to suggest appropriate means of treatment such as volume
reduction or the utilization of thermal technologies to meet future expected enforcement. Also, some concerns on hazardous
pollutant release such as that of dioxins while utilizing such thermal treatment methods were considered. The present on-going
research and development projects to meet such future management targets are also introduced. 相似文献