Heteroduplex formation: A potential source of genotyping error from PCR products

The formation of heteroduplexes from polymerase chain reaction (PCR) products has recently become a diagnostic tool that is routinely used for the prenatal detection of small deletions or insertions in a number of disease-causing alleles. We present evidence illustrating that heterozygous PCR products can manifest ‘invisible’ heteroduplexes that can ultimately lead to genotyping errors. Justifications for these ‘invisible’ heteroduplexes and requisite parameters to optimize their detection are presented.     

Ranked set sampling: an annotated bibliography

The paper provides an up-to-date annotated bibliography of the literature on ranked set sampling. The bibliography includes all pertinent papers known to the authors, and is intended to cover applications as well as theoretical developments. The annotations are arranged in chronological order and are intended to be sufficiently complete and detailed that a reading from beginning to end would provide a statistically mature reader with a state-of-the-art survey of ranked set sampling, including historical development, current status, and future research directions and applications. A final section of the paper gives a listing of all annotated papers, arranged in alphabetical order by author.This paper was prepared with partial support from the United States Environmental Protection Agency under a Cooperative Agreement Number CR-821531. The contents have not been subject to Agency review and therefore do not necessarily reflect the views or policies of the Agency and no official endorsement should be inferred.     

观测误差对非线性环境模型参数识别的影响

采用SCE-UA方法和RSA方法，比较了在不同的观测误差条件下，优化方法和不确定性分析方法对于非线性模型参数识别的影响。分析表明，RSA方法是在具有观测误差的数据条件下进行参数识别的一种有效手段。同时，通过比较分析，发现RSA方法预测的浓度平均值和概率最大值与“真实值”并不完全一致。因此，在使用RSA方法时，应该充分考虑预测区间，以降低决策风险。     

人员可靠性与系统安全

论述了人员可靠性对系统安全的影响，分析了它的特点和应采用的研究方法，介绍了笔者近年来在核电站人员可靠性基础研究方面的情况，并对研究的发展趋势进行了展望。     

施工过程中人因差错控制效率分析

以一个钢筋砼单筋梁受弯构件正截面承载力失效概率为例,通过数值模拟发现:检查抽样率为100%的首次检查对控制人因差错非常有效,提高取样率可以有效减少和降低人因差错的发生及其影响程度,但随检查次数增加检查效率直线下降。当对各种工序进行比较细致的检查时,适当提高结构参数设计值可有效防止由较难检查出来的微小人因差错引起的结构失效。显然,质检方法、检查次数、取样率和提高结构参数设计值对人因差错控制效率的提高是十分重要的;人因差错控制效率与检查人力、物力投入成正比。上述结论对合理控制施工过程中人因差错的方案选择具有一定的现实意义。     

防洪预报调度方式下水库下游防洪风险分析--以葠窝水库为例

水库实施的防洪预报调度方式是解决我国防洪和兴利之间矛盾的一条有效途径,其对水库上下游的防洪风险率的大小是决定其能否实施的关键.目前对此缺少一种定量计算方法.本文在分析防洪预报调度方式风险指标的前提下,根据水库洪水径流深与产流预报误差的分布规律,采用数值分析方法计算水库实施防洪预报调度方式时,相对于常规调度方式,产流预报误差对水库下游防洪风险率,并以实例论述了具体分析思路和计算过程.     

Using interview surveys and multispecies occupancy models to inform vertebrate conservation

Species distribution data are an essential biodiversity variable requiring robust monitoring to inform wildlife conservation. Yet, such data remain inherently sparse because of the logistical challenges of monitoring biodiversity across broad geographic extents. Surveys of people knowledgeable about the occurrence of wildlife provide an opportunity to evaluate species distributions and the ecology of wildlife communities across large spatial scales. We analyzed detection histories of 30 vertebrate species across the Western Ghats biodiversity hotspot in India, obtained from a large-scale interview survey of 2318 people who live and work in the forests of this region. We developed a multispecies occupancy model that simultaneously corrected for false-negative (non-detection) and false-positive (misidentification) errors that interview surveys can be prone to. Using this model, we integrated data across species in composite analyses of the responses of functional species groups (based on disturbance tolerance, diet, and body mass traits) to spatial variation in environmental variables, protection, and anthropogenic pressures. We observed a positive association between forest cover and the occurrence of species with low tolerance of human disturbance. Protected areas were associated with higher occurrence for species across different functional groups compared with unprotected lands. We also observed the occurrence of species with low disturbance tolerance, herbivores, and large-bodied species was negatively associated with developmental pressures, such as human settlements, energy production and mining, and demographic pressures, such as biological resource extraction. For the conservation of threatened vertebrates, our work underscores the importance of maintaining forest cover and reducing deforestation within and outside protected areas, respectively. In addition, mitigating a suite of pervasive human pressures is also crucial for wildlife conservation in one of the world's most densely populated biodiversity hotspots.     

基于TEM模型的民航不安全事件关联规则挖掘与分析*

为了预防民航不安全事件的发生,应用机组威胁与差错管理（TEM）模型分析2014—2020年民航事故/征候的航空安全报告资料,提取事件里存在于民航运行风险中潜在的情况、威胁、机组差错等因素,通过改进的关联规则方法挖掘其中的关联关系,包括挖掘与事件严重程度有关的因素,找到TEM模型中的关键因素和影响航空器结束状态的致因因素,并进行关联网络图分析。研究结果表明:手动操纵/飞行控制差错、缺少/不足的飞行培训和安全管理、飞行员之间沟通差错与程序执行错误是造成事故/征候的显著因素;关联规则能够有效利用航空安全报告信息,通过定量的方法挖掘事故/征候的特征,找到影响民航不安全事件的强关联因素,为民航安全管理人员提供决策依据。     

民航安全中人的因素研究方法综述

分析民航业中人的因素研究方法,指出其存在的不足。在此基础上,对核工业中成功应用的人的可靠性分析技术进行系统的研究。结合其优势,提出在民航安全工作中进行人的可靠性研究的观点。并将民航业与核工业中的多项内容进行对比,论证研究其可行性。为民航安全中人的因素研究提供新的理论指导和可靠的数据支持,最终使人因分析结果全面、深入和准确。