镧、铈对厌氧颗粒污泥产甲烷的Hormesis效应及其动力学研究

间歇培养条件下研究了单一稀土元素镧(La3 )和铈(Ce3 )在不同浓度水平下对厌氧颗粒污泥活性及其动力学行为的影响.结果表明,La3 和Ce3 对厌氧颗粒污泥比产甲烷活性有促进作用的浓度范围均为0.01～0.1 mg·L-1,促进作用浓度峰值均为0.05mg·L-1,最大促进百分比分别为10.35%和20.79%;La3 和Ce3 对SMA(比甲烷活性)的半抑制浓度分别为5mg·L-1和1000mg·L-1,La3 较易对污泥产生抑制作用.米-门方程可以用来描述厌氧消化过程中甲烷发酵阶段的动力学行为,稀土元素La3 和Ce3 投加至反应系统后,提高了甲烷发酵过程的动力学常数Vmax和半饱和常数Ks.     

臭氧室内空气质量评价标准的研究制订

通过分析O3毒理学研究和流行病学研究结果，参考国际权威机构制订的O3健康基准和国外相关标准，在考虑O3室内污染特点和我国目前污染水平的基础上，提出我国室内空气质量标准推荐值。     

应用δ~(13)C值探讨土壤中有机碳的迁移规律

C3 植物与C4 植物具明显的δ13C值差异 ,利用这种特性 ,选取荔波茂兰喀斯特森林边缘处这个C3植物—C4 植物转换生长的地点 ,分别采集森林与农田各三个剖面的土壤样品 ,根据测定其土壤总有机碳、不同粒径及不同比重组分的δ13C值间的相互关系来推导土壤有机碳的若干迁移规律     

银柱吸附,离子色谱法测定海水中的NO3--N

利用银柱吸附海水中的Cl-离子,然后通过电导法离子色谱测定海水中的NO3-_N。研究结果表明,使用该方法测定海水中的NO3-_N,分析方法简单,测试结果准确,同一样品多次测定结果的相对标准偏差低于2.0%,检测结果不受干扰,检出限为0.008mgL,是监控海水中NO3-_N的有效方法。     

一个研究街道峡谷流场及浓度场特征的三维数值模式

目前，研究街道峡谷内流场及机动车排放污染物的扩散行为特征所采用的主要方法为：采用野外测试法和物理模拟法，而采用三维数值模拟方法研究此问题的工作很少。本文创建了一个研究微尺度街道峡谷内流场及机动车排放污染物扩散特征的三维数值模式，即首次采用伪不定常方法，利用K——E闭合方案，建立了一个模拟城市街道峡谷内流场及污染物扩散特征与街道峡谷风场、街道几何结构及两侧建筑物高度对称性之间的复杂关系的三维数值模式。经过与实际监测资料及风洞实验对比，结果表明此三维模式具有较好的模拟精度，能够很好模拟峡谷内的风场及街谷几何结构对街道峡谷内流场及浓度场特征的影响，有很强的实用性。     

CUMULATIVE IMPACTS OF LANDUSE ON WATER QUALITY IN A SOUTHERN APPALACHIAN WATERSHED1

ABSTRACT: Water quality variables were sampled over 109 weeks along Coweeta Creek, a fifth-order stream located in the Appalachian mountains of western North Carolina. The purpose of this study was to observe any changes in water quality, over a range of flow conditions, with concomitant downstream changes in the mix of landuses. Variables sampled include pH, HCO₃^2?, conductivity, NO₃^?_?-N, NH₄⁺-N, PO₄^3?-P, C1^?-, Na, K, Ca²⁺, Mg²⁺, SO₄^2?, 5iO₂, turbidity, temperature, dissolved oxygen, total and fecal coliform, and focal streptococcus. Landcover/landuse was interpreted from 1:20,000 aerial photographs and entered in a GIS, along with information on total and paved road length, building location and density, catchment boundaries, hydrography, and slope. Linear regressions were performed to relate basin and near-stream landscape variables to water quality. Consistent, cumulative, downstream changes in water quality variables were observed along Coweeta Creek, concomitant with downstream, human-caused changes in landuse. Furthermore, larger downstream changes in water quality variables were observed during stormflow when compared to baseflow, suggesting cumulative impacts due to landscape alteration under study conditions were much greater during storm events. Although most water quality regulations, legislation, and sampling are promulgated for baseflow conditions, this work indicates they should also consider the cumulative impacts of physical, chemical, and biological water quality during stormflow.     

Enhanced production of poly(3-hydroxybutyrate) by filamentation-suppressed recombinantEscherichia coli in a defined medium

Fed-batch cultures of recombinantEscherichia coli strains were carried out for the production of poly(3-hydroxybutyric acid) (PHB) in a chemically defined medium. TheE. coli strains used were XL1-Blue, harboring pSYL105, a stable high-copy number plasmid containing theAlcaligenes eutrophus polyhydroxyalkanoate (PHA) genes, and XL1-Blue, harboring pSYL107, which is pSYL105 containing theE. coli ftsZ gene to suppress filamentation. With XL1-Blue(pSYL105) the final cell mass and PHB concentration obtained in 62 h were 102 and 22.5 g/L, respectively. Fed-batch culture of XL1-Blue(pSYL107) under identical conditions resulted in a final cell mass and PHB concentration of 127.5 and 48.2 g/L, respectively. The PHB contents obtained with XL1-Blue(pSYL105) and XL1-Blue(pSYL107) were 22.1 and 37.8%, respectively. Therefore, PHB was more efficiently produced in a defined medium by employing filamentation-suppressed recombinantE. coli.     

Synthesis of Short-chain-length/Medium-chain-length Polyhydroxyalkanoate (PHA) Copolymers in Peroxisome of the Transgenic Arabidopsis Thaliana Harboring the PHA Synthase Gene from Pseudomonas sp. 61-3

In this paper, the photosynthetic production of short-chain-length/medium-chain-length polyhydroxyalkanoate (PHA) copolymers is reported. The wild-type and highly active doubly mutated PHA synthase 1 (S325T/Q481K, abbreviated ST/QK) genes from Pseudomonas sp. 61-3 were introduced into Arabidopsis thaliana. Peroxisome targeting signal 1 (PTS1) was used to target PHA synthases into the peroxisome to synthesize PHA from the intermediates of the β-oxidation pathway. The transgenic Arabidopsis produced PHA copolymers consisting of 40–57 mol% 3-hydroxybutyrate, 21–49 mol% 3-hydroxyvalerate, 8–18 mol% 3-hydroxyhexanoate, and 2–8 mol% 3-hydroxyoctanoate. The maximum PHA contents were 220μ g/g cell dry weight (cdw) in leaves, and 36μ g/g cdw in stems, respectively. The expression of the ST/QK mutated PHA synthase in leaves gene did not lead to significant difference in PHA content and monomer composition of PHAs, compared to the wild-type PHA synthase gene, suggesting that the supply of monomers may be a rate-determining step of PHA biosynthesis in the peroxisome. However, in stems, there were significant differences dependent on whether the wild-type or ST/QK mutated PHA synthase was expressed. These results suggest that tissue-specific monomer availability is important in determining the final mol% composition of PHA copolymers produced by the peroxisome in plants.     

青藏铁路救援指挥系统的研究

通过对青藏铁路特点及运营模式的分析,提出构建青藏铁路事故救援指挥系统的重要性。结合青藏铁路信息化总体规划,在充分利用青藏铁路各信息系统及通讯网络基础上,利用地理信息系统(GIS)技术和无线通讯技术,建设具有指挥、控制、通信及情报(C3I)功能的青藏铁路事故救援指挥系统。提出了系统应达到的目标功能,结合青藏铁路生产作业实际情况,给出了系统实现的信息流程、体系结构,并开发了原型系统。系统将青藏铁路多源信息整合并结合GIS技术以直观的电子地图、图像等方式展示出来,实现了救援预案管理和智能化查询、匹配;并通过应急无线通讯设备实现事故现场视频、图像实时播放及指挥调度与事故现场间的语音双向通讯;同时可建立事故数据库,为统计报告和事故分析服务。     

UV/O3复合降解水中2,4-二氯酚的试验研究

以紫外光源为主要依托,变换反应器中的不同工艺条件,分别对紫外光、臭氧和紫外/臭氧三种不同于工艺条件对水中污染物2,4-二氯酚(2 ,4-DCP)的降解规律进行研究.结果表明,UV/O3复合对2,4-DCP的降解较UV、O3单独作用效果好;溶液的酸碱度影响UV/O3对2 ,4-DCP的降解;此外,溶液中含有细菌时,E. coli的杀灭效果仍表现为UV/O3＞O3＞UV.细菌的杀灭和2,4-DCP的降解均消耗羟基自由基,形成竞争反应,从而影响到2,4-DCP的降解效果.