In vitro toxicity of fungicides with different modes of action to alfalfa anthracnose fungus,Colletotrichum destructivum

Abstract

Sensitivity of 24 isolates of Colletotrichum destructivum O’Gara, collected from alfalfa plants in Serbia, to eight selected fungicides, was investigated in this study. Molecular identification and pathogenicity test of isolates tested were also performed. Fungicide sensitivity was evaluated in vitro, using mycelial growth assay method. All isolates exhibited significant pathogenicity, causing necrosis at the alfalfa seedling root tips two days after inoculation. Using the primer pair GSF1-SR1 and by comparing the amplified fragments of the tested isolates with the marker (M), the presence of the amplicon of the expected size of about 900?bp was determined for all isolates. The isolates tested in this study showed different sensitivity towards fungicides in vitro. Mycelial growth was highly inhibited by QoI (quinone outside inhibitors) fungicide pyraclostrobin (mean EC₅₀=0.39?µg mL^?1) and by DMI (demethylation-inhibiting) fungicide tebuconazole (mean EC₅₀=0.61?µg mL^?1), followed by azoxystrobin (mean EC₅₀=2.83?µg mL^?1) and flutriafol (mean EC₅₀=2.11?µg mL^?1). Multi-site fungicide chlorothalonil and MBC (methyl benzimidazole carbamate) fungicide thiophanate-methyl evinced moderate inhibition with mean EC₅₀=35.31 and 62.83?µg mL^?1, respectively. Thirteen isolates were sensitive to SDHI (succinate dehydrogenase inhibitors) fungicide boscalid and fluxapyroxad, (mean EC₅₀=0.49 and 0.19?µg mL^?1, respectively), while the rest of isolates were highly resistant.     

社会产品价格对水价的敏感性——基于虚拟水贸易框架的分析

虚拟水贸易是缺水地区从社会经济系统层面解决水资源短缺问题的一种策略选择,但由于长期的低水价,致使很多国家和地区的现实经济模式与虚拟水贸易战略的预期相悖.文章基于水资源投入产出分析方法,构建虚拟水贸易理论框架下的水价敏感性模型,分析水价上涨对各产业产品价格带来的影响.模型结果显示,区域水价提升对各产业产品价格的影响不仅在于该产业直接用水成本的上升,同时也不得不承受其他产业成本上升带来的间接影响,并且这两种影响作用是累加的.宁夏数据的实证得出,水价上涨对农业产品价格的影响最为强烈,批发和零售贸易餐饮业次之,对服务业产品价格的影响最弱.因此,可通过制定合理的水价调整策略,激励水资源极度缺乏的地区实施虚拟水贸易战略,避免过分强调传统的重农发展模式,促进产业结构向先进制造业和服务业转变,实现有限水资源更为合理的利用模式,达到经济发展与水生态的和谐局面.     

珠江三角洲潮土和水稻土酸缓冲特性试验研究

珠江三角洲潮土和水稻土对不同种类的酸具有一定的缓冲性,不同种类的酸对不同类型土壤的酸化趋势不同。大旺水稻土对酸的敏感性强于龙山潮土,龙山潮土属于稍易受害的稍敏感土壤,大旺水稻土属于易受害的敏感土壤;同一土壤对酸的缓冲能力比对碱的缓冲能力强;不同种类的酸对土壤的敏感性不同,土壤对硝酸的敏感性强于对硫酸的敏感性;土壤加酸后酸缓冲曲线和土壤中铝释放曲线的交点可以作为衡量土壤对酸敏感性的指标,交点越低,敏感性越强;加酸后土壤中铝的释放可以用模型y=nx+k来预测。     

核电厂核事故应急疏散研究

根据我国法规、标准与美国核管委对核电厂核事故应急疏散的要求,考虑我国核电厂特殊的厂址条件与社会环境,分析核事故应急疏散的可行性。通过应急疏散行为研究、厂区的交通需求估计和路网分析,对不同情境,考虑核事故应急疏散的特殊问题,分别采用人工容量分析与宏观仿真模拟得到应急疏散时间。针对某核电厂,给出核事故应急疏散分析的一般步骤、方法与计算结果。对宏观仿真模型的主要参数进行灵敏度分析,找出影响应急疏散时间的主要因素,提出缩短应急疏散时间的针对性措施。     

Effect of Planning for Connectivity on Linear Reserve Networks

Although the concept of connectivity is decades old, it remains poorly understood and defined, and some argue that habitat quality and area should take precedence in conservation planning instead. However, fragmented landscapes are often characterized by linear features that are inherently connected, such as streams and hedgerows. For these, both representation and connectivity targets may be met with little effect on the cost, area, or quality of the reserve network. We assessed how connectivity approaches affect planning outcomes for linear habitat networks by using the stock‐route network of Australia as a case study. With the objective of representing vegetation communities across the network at a minimal cost, we ran scenarios with a range of representation targets (10%, 30%, 50%, and 70%) and used 3 approaches to account for connectivity (boundary length modifier, Euclidean distance, and landscape‐value [LV]). We found that decisions regarding the target and connectivity approach used affected the spatial allocation of reserve systems. At targets ≥50%, networks designed with the Euclidean distance and LV approaches consisted of a greater number of small reserves. Hence, by maximizing both representation and connectivity, these networks compromised on larger contiguous areas. However, targets this high are rarely used in real‐world conservation planning. Approaches for incorporating connectivity into the planning of linear reserve networks that account for both the spatial arrangement of reserves and the characteristics of the intervening matrix highlight important sections that link the landscape and that may otherwise be overlooked. El Efecto de la Planeación para la Conectividad en Redes de Reservas Lineales     

复杂社会技术系统安全事故的成因结构敏感性及预防对策研究

简要回顾现有复杂社会技术系统安全事故的成因理论存在的局限性,根据大量统计资料和经验总结构建事故成因理论的缺陷。该研究试图从分析和推理入手,根据复杂社会技术系统运行机制及事故成因结构敏感性特征,探索由于新技术快速普及应用而不断涌现的复杂社会技术系统的失效机制及事故的成因理论;解释复杂社会技术系统安全事故的严重程度差异性、时间方向性及情境依赖性;为任何因新技术普及应用而产生的人造系统的安全分析及事故预防提供理论和方法支撑。     

Improving lake riparian source area management using surface and subsurface runoff indices

Sensitivity indices, which rank factors pertinent to surface and subsurface runoff pathways, were used to identify phosphorus source areas in riparian zones of 15 northern Minnesota lakes. Watershed models were first developed using a geographic information system (GIS). Empirical models were then developed correlating water quality with land use, lake morphometry, and riparian sensitivity. Base models of forested, cultivated, pasture/open, wetland and residential land use within 100, 200, 400, and 2000 m of the study lakes were regressed on total phosphorus and chlorophyll-a. Area-weighted groundwater and surface runoff sensitivity indices were then incorporated into each model and tested for significance. Within the 200-m buffer, the total phosphorus base model was improved by including the groundwater index alone. The chlorophyll-a base model at 200 m was improved by including: (1) the groundwater index alone, and (2) both the groundwater and surface runoff sensitivity indices. Results suggest that surface and subsurface runoff analysis of potential source areas can improve decision making for lake riparian management.     

COMPARATIVE WATER CHEMISTRY OF FOUR LAKES IN ROCKY MOUNTAIN NATIONAL PARK1

ABSTRACT: The inorganic chemistry of two pairs of lakes in Rocky Mountain National Park was studied to determine reasons for their similarities and differences. The pairs were located on differing geologic units. It was expected that weathering from the different types of parent material would cause differing cation concentrations between the pairs. This was verified by dissimilar concentrations of those cations which are products of primary weathering. Unexpected was a significant difference in anion concentrations between members of one pair having the same bedrock geology. This difference has been attributed to the presence of a wet sedge meadow above one of the lakes which serves as a biological filter for anions, particularly nitrate and sulfate. It is shown that small scale drainage characteristics which can alter regional atmospheric contributions are important contributors to lake chemistry.     

ACCURACY AND RELIABILITY OF NUMERICAL RIVER MODELS1

ABSTRACT: Over the last 20 years, our possibilities to model river flows numerically have increased enormously. In this paper, the question is addressed whether a more sophisticated model is always better than a simpler one. Increased detail, both in finer resolution and in physical processes taken into account, has its price in more computer time, higher data need, and perhaps more unknown coefficients to be calibrated. Moreover, uncertainty in actual physical conditions (e.g., bottom roughness), inflow and parameters remains, which may dominate the uncertainty of the results. Also, the questions asked by a decision maker may not always be very precise. For a schematic but relevant example, we show that a better model does not necessarily give more reliable results because some of the basic uncertainties remain. It is concluded that we should use the simplest model that will answer the question as to the accuracy needed, taking into account uncertainties in the data     

Sensitivity of Ozone Production to the Nmhc Composition, Aerosol Back-Scattering and High Chimney Emission

In this paper, three sensitivity studies are designed to analyze the effect of the NMHC (Non-Methane HydroCarbon) composition, the aerosol back-scattering and the high chimney NO_x emission to the photochemical prodution of ozone by using a one-dimensional photochemistry-diffusion model under a favourable meteorological condition. Measurements of the NMHC composition in Taipei indicated that the percentage of iso-butene, cis-2-butene, trans-2-butene and benzene in a unit volume was much higher than of those observed in other major cities. the high ratio of benzene was directly linked to its high percentage in gasoline. As to the unusually high amount of iso-butene, cis-2-butene and trans-2-butene, more researches are needed to identify their source. Concerns are raised as to how productive is NMHC composition is to the photochemical production of the surface ozone. A rough estimate shows that the total reactivity of the Taipei NMHC composition is about 1.21 × 10^-9 cm³ s^-1 which is 1.6 times that of the Los Angeles (LA) NMHC composition, while the simulated noon peak will be different by 28 ppbv, i.e. 18% more than that simulated with a LA composition.

Meanwhile, high aerosol loading is a serious problem in Taipei. the attenuation of the UV radiation by aerosols cannot be ignored. A numerical simulation shows that the noon ozone level will decrease from 178 to 141ppbv, i. e. about 21% reduction, with deterioration of the visual range from 85 to 5 km.

In the southern Taiwan, industry parks are mixed with the populated Kaohsiung city, hence the large emission of NO_x from high chimneys cannot be ignored. in this study, NO_x is assumed to be emitted in the layer between 235-460 m high with an emission rate of 0.05 or 0.145 ppbv/sec. the results show that the NO_x emitted from the elevated stack lead to a considerable reduction of surface ozone. Such conclusion is obtained due to the fact that a one-dimensional model is used in this paper. Whereas, if a three-dimensional regional model was used, then a higher productivity of ozone downstream would be simulated.