Uptake of cadmium into cells in culture∗

Cadmium has been recognized as pollutant of the environment for many years and numerous studies on its toxic effects have been carried out. Little, however, is known about its metabolic behaviour e.g. why the metal is accumulated so extremely rapidly into the organs of men and animals. Since the study of the individual metabolic steps is very difficult in vivo cell cultures may be used to obtain first indications of what happens in the whole animal.

We used CHO cells in monolayer culture to study the conditions under which the uptake of cadmium occurs. From serumfree medium the metal is accumulated rapidly in the cells. The uptake is inhibited very strongly by the presence of serum or albumin. Accumulation occurs against a concentration gradient and is dependent on the incubation temperature. Below 10°C no cadmium uptake is seen. Several substances which are known to affect cell metabolism have been used to influence cadmium accumulation. Neither inhibitors of energy production nor microtubule or microfilament disruptors showed any substantial effect. In contrast SH‐group blocking agents markedly reduced cadmium uptake.

The results show that cadmium uptake does not occur by passive diffusion but by some active mechanism.     

Interacting Effects of Translocation,Artificial Propagation,and Environmental Conditions on the Marine Survival of Chinook Salmon from the Columbia River,Washington, U.S.A.

Abstract: Captive rearing and translocation are often used concurrently for species conservation, yet the effects of these practices can interact and lead to unintended outcomes that may undermine species’ recovery efforts. Controls in translocation or artificial‐propagation programs are uncommon; thus, there have been few studies on the interacting effects of these actions and environmental conditions on survival. The Columbia River basin, which drains 668,000 km² of the western United States and Canada, has an extensive network of hydroelectric and other dams, which impede and slow migration of anadromous Pacific salmon (Oncorhynchus spp.) and can increase mortality rates. To mitigate for hydrosystem‐induced mortality during juvenile downriver migration, tens of millions of hatchery fish are released each year and a subset of wild‐ and hatchery‐origin juveniles are translocated downstream beyond the hydropower system. We considered how the results of these practices interact with marine environmental conditions to affect the marine survival of Chinook salmon (O. tshawytscha). We analyzed data from more than 1 million individually tagged fish from 1998 through 2006 to evaluate the probability of an individual fish returning as an adult relative to its rearing (hatchery vs. wild) and translocation histories (translocated vs. in‐river migrating fish that traveled downriver through the hydropower system) and a suite of environmental variables. Except during select periods of very low river flow, marine survival of wild translocated fish was approximately two‐thirds less than survival of wild in‐river migrating fish. For hatchery fish, however, survival was roughly two times higher for translocated fish than for in‐river migrants. Competition and predator aggregation negatively affected marine survival, and the magnitude of survival depended on rearing and translocation histories and biological and physical conditions encountered during their first few weeks of residence in the ocean. Our results highlight the importance of considering the interacting effects of translocation, artificial propagation, and environmental variables on the long‐term viability of species.     

APPLICATION OF ENHANCED ANNEALING TO GROUND WATER REMEDIATION DESIGN1

ABSTRACT: A methodology for ground water remediation design has been developed that interfaces ground water simulation models with an enhanced annealing optimizer. The ground water flow and transport simulators provide the ability to consider site‐specific contamination and geohydrologic conditions directly in the assessment of alternative remediation system designs. The optimizer facilitates analysis of tradeoffs between technical, environmental, regulatory, and financial risks for alternative design and operation scenarios. A ground water management model using an optimization method referred to as “enhanced annealing” (simulated annealing enhanced to include “directional search” and “memory” mechanisms) has been developed and successfully applied to an actual restoration problem. The demonstration site is the contaminated unconfined aquifer referred to as N‐Springs located at Han‐ford, Washington. Results of the demonstration show the potential for improving groundwater restoration system performance while reducing overall system cost.     

Conversion of a Missouri River Dam and Reservoir to a Sustainable System: Sediment Management1

Abstract: A present and future challenge for water resources engineers is to extend the useful life of our dams and reservoirs. Ongoing reservoir sedimentation in impoundments must be addressed; sedimentation in many reservoirs already limits project benefits and effective project life. Sustainability requires that incoming sediment be moved downstream past the impounding dam. We use Lewis and Clark Lake, the most downstream of the six Missouri River main stem reservoirs, to demonstrate how a reservoir in advanced stages of its project life could be converted to a sustainable system with local benefits exceeding costs by a factor of 1.5. Full consideration of benefits would further enhance project justification. The proposed strategy involves four phases that will take about 50 years to complete. Cost estimates for this potential project range from the quantitative to the plausible, but it is clear that the results justify a full engineering, environmental, and economic study of this model project. If implemented, the project will create scientific knowledge and develop technologies useful for achieving sustainability at many other reservoirs in the Mississippi River basin and beyond.     

Sorption and desorption kinetics of diuron,fluometuron, prometryn and pyrithiobac sodium in soils

Abstract

The sorption and desorption characteristics of four herbicides (diuron, fluometuron, prometryn and pyrithiobac‐sodium) in three different cotton growing soils of Australia was investigated. Kinetics and equilibrium sorption and desorption isotherms were determined using the batch equilibrium technique. Sorption was rapid (> 80% in 2 h) and sorption equilibrium was achieved within a short period of time (ca 4 h) for all herbicides. Sorption isotherms of the four herbicides were described by Freundlich equation with an r² value > 0.98. The herbicide sorption as measured by the distribution coefficient (K_d) values ranged from 3.24 to 5.71 L/kg for diuron, 0.44 to 1.13 L/kg for fluometuron, 1.78 to 6.04 L/kg for prometryn and 0.22 to 0.59 L/kg for pyrithiobac‐sodium. Sorption of herbicides was higher in the Moree soil than in Narrabri and Wee Waa soils. When the K_d values were normalised to organic carbon content of the soils (K_oC), it suggested that the affinity of the herbicides to the organic carbon increased in the order: pyrithiobac‐sodium < fluometuron < prometryn < diuron. The desorption isotherms were also adequately described by the Freundlich equation. For desorption, all herbicides exhibited hysteresis and the hysteresis was stronger for highly sorbed herbicides (diuron and prometryn) than the weakly sorbed herbicides (fluometuron and pyrithiobac‐sodium). Hysteresis was also quantified as the percentage of sorbed herbicides which is not released during the desorption step ω = [n_ad / n_de ‐1] x 100). Soil type and initial concentration had significant effect on ω. The effect of sorption and desorption properties of these four herbicides on the off‐site transport to contaminate surface and groundwater are also discussed in this paper.     

EXPOSURE ASSESSMENT OF POPULATIONS USING ENVIRONMENTAL MODELING,DEMOGRAPHIC ANALYSIS,AND GIS1

ABSTRACT: Simulation of ground-water flow and fate of contaminants in the subsurface environment constitutes a major phase of most environmental assessment and site remediation studies. These simulation studies yield information on spatial and temporal distributions of contaminants in the subsurface media. An important use of this information is to conduct exposure assessment studies. Spatial and temporal distributions of both chemical concentrations and exposed populations render this integrated exposure analysis task rather difficult. Geographic Information Systems (GIS), on the other hand, provide a platform in which layered, spatially distributed databases can be manipulated with ease, thereby simplifying exposure analysis tasks significantly. In this paper, we describe procedures that combine the simulation models and demographic databases under a GIS platform to automate the exposure assessment phase of a typical health assessment study. Procedures developed herein significantly simplify the post-processing phase of the analysis, and render the overall task more ‘user friendly.’ A site-specific application is included as a demonstration of the proposed process.     

Morphologie effects of channelization: the case of the Neebing-McIntyre floodway,Thunder Bay,Ontario, Canada

The Neebing-McIntyre floodway in Thunder Bay, Ontario, Canada, has been constructed with a relatively straight and uniform trapezoidal channel, compared with the prechannelized sinuous reaches of the Neebing and the McIntyre rivers. The flow regime of the floodway also contrasts significantly with the prechannelized regime, because of the combination of discharges from these rivers into a new channel and the regulation of flows by a diversion structure. The maximum channel capacity of the floodway is about 284 m³ s^–1 (175-year regional flood), compared with about 40 m³ s^–1 and 60 m³ s^–1, respectively, for the Neebing and the McIntyre. According to regime theories, the construction of a straight and trapezoidal channel has upset the equilibrium of the stream system and therefore should lead to some accelerated erosion and sedimentation processes in the new channel immediately after construction. Erosion potential is particularly high during higher discharge events, when flow velocities are expected to be greater than the prechannelized velocities of the Neebing and the McIntyre. The overall sediment yield of the watershed is low (71t km^–2 yr^–1), compared with other documented watersheds of North America, but the rates of deposition in the floodway are relatively high, mainly due to the backwater effect of Lake Superior. Unless maintained by constant channel work, the floodway will tend to fill up with sediment, until a postconstructional equilibrium is reestablished.     

VARIABILITY IN MEASURED BEDLO AD-TRANSPORT RATES1

ABSTRACT: Variability in bedload-transport rates during constant water discharge is an inherent part of the bedload-transport process. Although this variability has been measured extensively in the laboratory, similar information generally is not available from field measurements. During a four-day period of nearly constant water discharge, four sets of consecutively collected bedload samples, ranging from 43 to 120 samples, were obtained at the same cross channel location using a standard 65-pound Helley-Smith bedload sampler. When the measured transport rates are converted to dimensionless rates and plotted as cumulative frequency distributions, they show good agreement with a theoretical probability distribution function of rates derived for the case of ripples on dunes. The distributions show that during constant water discharge individual measured rates at a fixed point vary from near zero to four times the mean rate, and 60 percent of the sampled rates will be less than the mean. Because of the large variation in transport rates that occurs at every location in the cross section, many observations are required to establish an accurate estimate of the mean rate at any given location.     

BEDLOAD TRANSPORT IN A POOL-RIFFLE SEQUENCE OF A COASTAL ALASKA STREAM1

ABSTRACT: A Helley-Smith pressure differential bedload sampler was used to measure bedload transport at consecutive riffle sections of a riffle-pool-riffle sequence on Bambi Creek, a small (154 ha), second-order stream on Chichagof Island, Alaska, during four storms over a 2-year period. Maximum bedload transport rate measured was 4920 kg/h at a streamflow of 2.35 m³/s corresponding to a storm having a 5-year return interval. Transport of larger sediment (> 8 mm) varied systematically with streamflow at the two sampling locations. At flows up to approximately bankfull, transport of large sediment was greatest at the upstream site; at flows above bankfull, transport of large sediment was greatest at the downstream site. The net import of large sediment to the pool during moderate stormflows and net export of large sediment from the pool during flows above bankfull may be related to a “convergence” or “reversal” of competence between the upstream riffle and subsequent pool at flows approximating bankfull stage. Cross-sections monitored within the study reach indicate that stormflows resulted in net filling of the riffle sections and net scour of the pool; periods of low streamflow resulted in net scour of the riffles and net filling of the pooL     

IDENTIFICATION OF AQUIFER DISPERSIVITIES: METHODS OF ANALYSIS AND PARAMETER UNCERTAINTY1

ABSTRACT: This paper presents a method for estimating aquifer dispersivities in solute transport models. Sensitivity equations are derived for the calculation of sensitivity coefficients. A modified Gauss-Newton algorithm is used to perform the least-squares minimization. A statistical procedure is outlined to assess reliability of the estimated parameters. The solute transport model is solved by the upstream weighted, multiple cell balance method which combines the concepts of local mass balance and finite element approximations. A one-dimensional solute transport problem in a vertical column system is first used to illustrate the inverse technique. A second example considers the parameter identification problem for three-dimensional solute transport with a unidirectional steady and uniform flow field. The third example solves the parameter identification problem in a three-dimensional, stream-aquifer, solute transport system with steady state flow. Numerical experiments are conducted to study data requirements for parameter identification.